##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552679_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458466
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705952022614547	31.0	31.0	34.0	30.0	34.0
2	31.817698586154698	31.0	31.0	34.0	30.0	34.0
3	31.838862641940732	33.0	31.0	34.0	30.0	34.0
4	35.53336561489838	37.0	35.0	37.0	33.0	37.0
5	35.43534307887608	37.0	35.0	37.0	33.0	37.0
6	35.38443854069877	37.0	35.0	37.0	33.0	37.0
7	35.78600157917926	37.0	35.0	37.0	35.0	37.0
8	35.616734937814364	37.0	35.0	37.0	35.0	37.0
9	37.43548921839351	39.0	37.0	39.0	35.0	39.0
10	36.823875707249826	39.0	37.0	39.0	32.0	39.0
11	36.72504831328823	39.0	35.0	39.0	32.0	39.0
12	36.700970191900815	39.0	35.0	39.0	32.0	39.0
13	36.55812862894958	39.0	35.0	39.0	32.0	39.0
14	37.66652925189654	39.0	36.0	41.0	33.0	41.0
15	37.83591585853695	40.0	37.0	41.0	33.0	41.0
16	37.91127150104915	40.0	37.0	41.0	33.0	41.0
17	37.887516195312195	40.0	36.0	41.0	33.0	41.0
18	37.80525055293086	39.0	36.0	41.0	33.0	41.0
19	37.76516688260416	39.0	36.0	41.0	33.0	41.0
20	37.65578690677171	39.0	36.0	41.0	33.0	41.0
21	37.510173055362884	39.0	35.0	41.0	33.0	41.0
22	37.59468095780276	39.0	36.0	41.0	33.0	41.0
23	33.47929399344771	39.0	34.0	41.0	0.0	41.0
24	35.210763720755736	39.0	35.0	41.0	18.0	41.0
25	36.28898762394594	39.0	34.0	41.0	29.0	41.0
26	36.90917538050804	39.0	35.0	40.0	31.0	41.0
27	30.604936898265084	38.0	31.0	40.0	0.0	41.0
28	33.2621503012219	38.0	31.0	40.0	16.0	41.0
29	35.24123271954736	38.0	32.0	40.0	25.0	41.0
30	36.263803640837054	38.0	35.0	40.0	30.0	41.0
31	36.62003071111053	38.0	35.0	40.0	30.0	41.0
32	35.065902378802356	38.0	35.0	40.0	26.0	41.0
33	35.73683326571654	39.0	35.0	40.0	26.0	41.0
34	36.15273760758704	39.0	35.0	40.0	30.0	41.0
35	36.36017719961786	39.0	35.0	40.0	30.0	41.0
36	36.396020642752134	39.0	35.0	40.0	30.0	41.0
37	36.42365191748134	39.0	35.0	40.0	30.0	41.0
38	36.40887873909952	39.0	35.0	40.0	30.0	41.0
39	36.405257968966076	39.0	35.0	40.0	30.0	41.0
40	36.35445158419599	39.0	35.0	40.0	30.0	41.0
41	36.31748919221927	39.0	35.0	40.0	30.0	41.0
42	36.27400723281552	39.0	35.0	40.0	30.0	41.0
43	36.244687719481924	39.0	35.0	40.0	30.0	41.0
44	36.17433790073855	39.0	35.0	40.0	30.0	41.0
45	36.129052099828556	39.0	35.0	40.0	30.0	41.0
46	36.04177627130474	38.0	35.0	40.0	30.0	41.0
47	35.94129990010164	38.0	35.0	40.0	29.0	41.0
48	35.93775329032033	38.0	35.0	40.0	29.0	41.0
49	35.95815611190361	38.0	35.0	40.0	29.0	41.0
50	35.85580828240262	38.0	35.0	40.0	29.0	41.0
51	35.13577669881736	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	15.0
10	36.0
11	27.0
12	21.0
13	23.0
14	20.0
15	24.0
16	46.0
17	80.0
18	168.0
19	383.0
20	582.0
21	923.0
22	1356.0
23	1923.0
24	2711.0
25	3571.0
26	4737.0
27	5634.0
28	6847.0
29	8407.0
30	11198.0
31	14162.0
32	18693.0
33	25188.0
34	36346.0
35	43260.0
36	41263.0
37	52803.0
38	76510.0
39	101448.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.94422705282398	18.372136646992363	21.766935825121163	14.916700475062491
2	32.62750127599429	25.50592628460998	25.298931654691952	16.567640784703773
3	31.334929962090975	25.051366949784715	28.0040395580043	15.609663530120008
4	27.928788612459808	26.973210663386162	26.77908503574965	18.318915688404374
5	24.569542779617244	31.139931859723514	25.415843268639332	18.87468209201991
6	24.186744491412668	36.780699114001905	24.937072760030187	14.095483634555233
7	80.65876204560425	6.749682637316616	8.587986895429541	4.003568421649588
8	82.24906536144448	5.630733794872466	7.89807750193035	4.222123341752714
9	75.87956358813958	8.65407685629905	10.072502650141995	5.393856905419376
10	36.27270070190592	30.30540977956926	19.201641997443648	14.22024752108117
11	27.643271256756226	27.99291550518468	27.185658260372637	17.178154977686457
12	24.74730950604843	24.62472680634987	31.78643563535791	18.841528052243785
13	23.11011067341962	26.939402267561825	32.93155871973058	17.01892833928797
14	20.70338912809238	30.830639567601526	30.71852656467437	17.747444739631728
15	20.120576007817373	26.559657640915574	35.69076005636187	17.629006294905185
16	21.925508107471437	25.959831263387034	32.60023644065208	19.51442418848944
17	22.908132773204557	25.880217944187788	29.19976617677211	22.011883105835548
18	22.929944641478322	25.47538966902671	31.870411328211905	19.724254361283062
19	23.719534272988618	26.738078723394977	29.9487857332932	19.59360127032321
20	23.601095828262075	27.881674976988478	29.290067311425496	19.227161883323955
21	22.105892258095476	28.53341360100858	30.507169561101588	18.85352457979436
22	21.33178905305955	26.789554732521058	29.582782583659423	22.295873630759967
23	17.2763083849184	35.6944680739684	27.53050389778086	19.49871964333233
24	19.748247416384203	27.36800547914131	31.722744979998517	21.16100212447597
25	19.303939659647607	29.139783539019255	30.028399052492443	21.527877748840698
26	19.220836441524565	29.826203033594638	30.360593806301882	20.592366718578916
27	17.24402681987323	41.68357086457883	24.864657357361285	16.207744958186648
28	18.49493746537366	28.200782609833663	33.193301139015766	20.11097878577692
29	20.856072206008733	27.06874664642525	30.736194177976124	21.338986969589893
30	22.030641312550987	28.971395915945784	29.44165979592816	19.556302975575072
31	22.101093647075245	28.328163920552452	28.967469779656508	20.603272652715795
32	20.553977830417086	32.64604136402699	26.936348606003495	19.86363219955242
33	22.99690707707878	28.229138038589557	27.29166394018313	21.48229094414853
34	20.106398293439426	28.171118468981344	30.587000998983566	21.135482238595664
35	20.853018544450403	28.835507976600226	28.065112789170843	22.24636068977852
36	23.34218895185248	27.58525168714801	28.71183468348798	20.360724677511527
37	20.634899861712753	28.838779756841294	30.275527520034203	20.25079286141175
38	20.716258130373898	29.043375081249213	28.30155344125846	21.938813347118437
39	21.329607866232177	28.192712218572368	28.93562445197681	21.542055463218645
40	22.319430448495638	26.80351432821627	28.918829313406007	21.958225909882085
41	19.836367364210215	26.881382697953608	28.376586268120207	24.905663669715967
42	21.02075181147566	26.71408566829383	28.740190112243873	23.52497240798663
43	20.983017279362045	27.362116274707393	29.05166359119324	22.60320285473732
44	20.977564312293605	28.23742654853359	28.42871663329451	22.356292505878297
45	20.941574729641893	28.217795867087197	29.0073854985975	21.83324390467341
46	22.685433598129414	27.537701814311205	28.662757979872005	21.114106607687376
47	20.213058329298136	27.26396286747545	30.194605488738535	22.32837331448788
48	20.800670060593372	26.734152587105697	29.92173901663373	22.543438335667204
49	21.575863859042983	24.690816767219378	30.594853271562123	23.138466102175514
50	20.765552952672607	26.387780118918307	29.745935358347186	23.100731570061903
51	20.56859178216051	25.67889440002094	28.119424341172518	25.63308947664603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1540.0
1	1638.0
2	1736.0
3	2411.0
4	3086.0
5	2163.5
6	1241.0
7	1305.0
8	1369.0
9	1393.5
10	1418.0
11	1451.5
12	1485.0
13	1511.0
14	1537.0
15	1508.0
16	1479.0
17	1481.0
18	1483.0
19	1455.0
20	1427.0
21	1464.0
22	1501.0
23	1840.5
24	2180.0
25	2860.5
26	4241.0
27	4941.0
28	6339.5
29	7738.0
30	8074.0
31	8410.0
32	10133.0
33	11856.0
34	12578.0
35	13300.0
36	15155.0
37	17010.0
38	18821.0
39	20632.0
40	23354.0
41	26076.0
42	28857.0
43	31638.0
44	35029.5
45	38421.0
46	41484.0
47	44547.0
48	45809.5
49	47072.0
50	45738.0
51	44404.0
52	39458.5
53	34513.0
54	29646.0
55	24779.0
56	21589.0
57	18399.0
58	15836.5
59	13274.0
60	11519.0
61	9764.0
62	8111.0
63	6458.0
64	5403.5
65	4349.0
66	3345.5
67	2342.0
68	1821.5
69	1301.0
70	1050.5
71	800.0
72	708.0
73	616.0
74	459.5
75	269.5
76	236.0
77	207.0
78	178.0
79	106.5
80	35.0
81	23.0
82	11.0
83	14.5
84	18.0
85	14.0
86	10.0
87	9.0
88	8.0
89	4.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458466.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.81540281355646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.65868797821076	27.85405074263011
2	10.036622349837986	7.3900458939365805
3	3.9589337166233745	4.372492184689792
4	1.9928993804741617	2.9347757383617354
5	1.2141616337800067	2.234992481418839
6	0.8434015881479187	1.8630101520755324
7	0.599176645439936	1.5441250690842723
8	0.4802833947419396	1.4145461313669483
9	0.4037735065339666	1.3378575859641137
>10	4.431473177831523	37.86468148863593
>50	0.3487728854489372	7.912131356274589
>100	0.028278882603967884	2.043372611844154
>500	0.0023565735503306567	0.6801843233991367
>1k	0.0011782867751653284	0.5537342403182386
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1324	0.2887891359446502	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1229	0.26806786108457337	No Hit
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	835	0.18212910008593877	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	833	0.18169286272046345	TruSeq Adapter, Index 22 (95% over 22bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	792	0.17274999672821978	No Hit
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	676	0.1474482295306522	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	485	0.10578756112776085	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17994791325856224	0.0
2	0.0	0.0	0.0	0.7669052885055817	0.0
3	0.0	0.0	0.0	1.001819109814032	0.0
4	0.0	0.0	0.0	1.2858096347384538	0.0
5	0.0	0.0	0.0	2.122294783037346	0.0
6	0.0	0.0	0.0	2.760291930044976	0.0
7	0.0	0.0	0.0	3.3684068175175477	0.0
8	0.0	0.0	0.0	4.42213817382314	0.0
9	0.0	0.0	0.0	4.892402053805517	0.0
10	0.0	0.0	0.0	6.040360681053775	0.0
11	0.0	0.0	0.0	7.399458193192079	0.0
12	0.0	0.0	0.0	8.634882412218136	0.0
13	0.0	0.0	0.0	9.042546230254807	0.0
14	0.0	0.0	0.0	9.245178486518084	0.0
15	0.0	0.0	0.0	9.547709099475206	0.0
16	0.0	0.0	0.0	10.22714879620299	0.0
17	0.0	0.0	0.0	10.950866585526517	0.0
18	0.0	0.0	0.0	11.86892812116929	0.0
19	0.0	0.0	0.0	12.30538360532733	0.0
20	0.0	0.0	0.0	12.734641172955028	0.0
21	0.0	0.0	0.0	13.2703406577587	0.0
22	0.0	0.0	0.0	13.773976696199936	0.0
23	0.0	0.0	0.0	14.298115890818512	0.0
24	0.0	0.0	0.0	14.71145079460636	0.0
25	0.0	0.0	0.0	15.057387025428277	0.0
26	0.0	0.0	0.0	15.439094720219165	0.0
27	0.0	0.0	0.0	15.77957798397264	0.0
28	2.1811868273765122E-4	0.0	0.0	16.209053670283076	0.0
29	2.1811868273765122E-4	0.0	0.0	16.63198579611138	0.0
30	2.1811868273765122E-4	0.0	0.0	17.13278629167703	0.0
31	2.1811868273765122E-4	0.0	0.0	17.593889186984423	0.0
32	2.1811868273765122E-4	0.0	0.0	18.041904961327557	0.0
33	2.1811868273765122E-4	0.0	0.0	18.49951795771115	0.0
34	2.1811868273765122E-4	0.0	0.0	18.966946294817937	0.0
35	2.1811868273765122E-4	0.0	0.0	19.450951651812783	0.0
36	2.1811868273765122E-4	0.0	0.0	19.892205746991053	0.0
37	2.1811868273765122E-4	0.0	0.0	20.33934904660324	0.0
38	2.1811868273765122E-4	0.0	0.0	20.786056108849948	0.0
39	2.1811868273765122E-4	0.0	0.0	21.274423839499548	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAAACG	35	1.2097917E-7	45.000004	1
TATTTCG	25	3.8871243E-5	45.0	28
TCGTTCG	20	7.0288463E-4	45.0	27
CTCCGTA	20	7.0288463E-4	45.0	14
CGAGTTA	25	3.8871243E-5	45.0	11
GCGAAAT	20	7.0288463E-4	45.0	7
TTCACCG	50	2.1827873E-11	45.0	23
CGATTAC	20	7.0288463E-4	45.0	10
TACGGGC	80	0.0	45.0	4
TAACGCA	85	0.0	45.0	26
TGCGGAT	20	7.0288463E-4	45.0	4
CGTTCGA	20	7.0288463E-4	45.0	28
TCGATAC	20	7.0288463E-4	45.0	11
AACGGAC	20	7.0288463E-4	45.0	41
GCCGATA	20	7.0288463E-4	45.0	9
TAACCGG	20	7.0288463E-4	45.0	26
CGCTCGT	50	2.1827873E-11	45.0	13
ACTGTCG	20	7.0288463E-4	45.0	15
CTCGAGG	45	3.8380676E-10	45.0	2
TATAGCG	25	3.8871243E-5	45.0	1
>>END_MODULE