FastQCFastQC Report
Sat 18 Jun 2016
SRR3552675_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552675_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704467
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26930.38227482621613224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17470.2479889050871084No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC15220.2160498646494442No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG13340.18936302197264032No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC13200.18737570390096342No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC11480.16296008187750455TruSeq Adapter, Index 20 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10380.1473454398857576No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC207.0314243E-445.00000415
CGTTCCC207.0314243E-445.00000422
ACTCGGA207.0314243E-445.00000410
TCGGTTA207.0314243E-445.00000431
CTAACGG453.8380676E-1045.02
CTAACCG253.8892627E-545.042
TGCGTAG253.8892627E-545.01
CTTACGA253.8892627E-545.029
CTATGCG302.164301E-644.9999961
CCCGTTA302.164301E-644.99999634
ACGATAG302.164301E-644.9999961
TTAACGG900.042.52
GTTAACG700.041.7857131
CATACGA1300.041.5384618
TACGAAT603.6379788E-1241.24999612
GTCGAAT556.002665E-1140.90909243
GCGATAC451.9264917E-840.09
GCTAAGT900.040.024
ATAGGGA5500.039.681824
ATTCGTG403.4556797E-739.3750043