##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552675_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	704467
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.753923178800427	31.0	31.0	34.0	30.0	34.0
2	31.87452925403177	33.0	31.0	34.0	30.0	34.0
3	31.908506715005814	33.0	31.0	34.0	30.0	34.0
4	35.588957325183436	37.0	35.0	37.0	33.0	37.0
5	35.475018702082565	37.0	35.0	37.0	33.0	37.0
6	35.413863246965434	37.0	35.0	37.0	33.0	37.0
7	35.741207182167514	37.0	35.0	37.0	35.0	37.0
8	35.509265870509196	37.0	35.0	37.0	33.0	37.0
9	37.35956403919559	39.0	37.0	39.0	34.0	39.0
10	36.88434660530586	39.0	37.0	39.0	33.0	39.0
11	36.772948910310916	39.0	37.0	39.0	32.0	39.0
12	36.609927789378354	39.0	35.0	39.0	32.0	39.0
13	36.41077438687689	39.0	35.0	39.0	32.0	39.0
14	37.52339144346009	40.0	36.0	41.0	32.0	41.0
15	37.67428850464252	40.0	36.0	41.0	33.0	41.0
16	37.76708348297365	40.0	36.0	41.0	33.0	41.0
17	37.68640830585393	40.0	36.0	41.0	33.0	41.0
18	37.67988280501429	39.0	36.0	41.0	33.0	41.0
19	37.595684396856065	39.0	36.0	41.0	33.0	41.0
20	37.45583540463925	39.0	35.0	41.0	32.0	41.0
21	37.31317577686393	39.0	35.0	41.0	32.0	41.0
22	37.375984964519276	39.0	35.0	41.0	32.0	41.0
23	33.27301917619988	38.0	34.0	41.0	0.0	41.0
24	34.93036295525553	38.0	34.0	41.0	17.0	41.0
25	36.00427273385411	38.0	34.0	40.0	27.0	41.0
26	36.60058455541566	38.0	35.0	40.0	30.0	41.0
27	30.42326326144447	38.0	31.0	40.0	0.0	41.0
28	32.979550497042446	38.0	31.0	40.0	16.0	41.0
29	34.90705029476185	38.0	31.0	40.0	25.0	41.0
30	35.927163373160134	38.0	34.0	40.0	30.0	41.0
31	36.326276461495006	38.0	35.0	40.0	30.0	41.0
32	34.771803363393886	38.0	34.0	40.0	25.0	41.0
33	35.44171267071417	38.0	35.0	40.0	25.0	41.0
34	35.824042857933726	38.0	35.0	40.0	28.0	41.0
35	35.99470805587771	38.0	35.0	40.0	30.0	41.0
36	36.04002742498939	38.0	35.0	40.0	30.0	41.0
37	36.053470212231375	38.0	35.0	40.0	30.0	41.0
38	35.96821426695644	38.0	35.0	40.0	30.0	41.0
39	35.93886867660231	38.0	35.0	40.0	30.0	41.0
40	35.89746290457892	38.0	35.0	40.0	29.0	41.0
41	35.7792600647014	38.0	35.0	40.0	29.0	41.0
42	35.70975077611868	38.0	35.0	40.0	29.0	41.0
43	35.65701729108674	38.0	35.0	40.0	29.0	41.0
44	35.58790972465708	38.0	35.0	40.0	29.0	41.0
45	35.497049542420015	38.0	34.0	40.0	28.0	41.0
46	35.398810732085394	38.0	34.0	40.0	28.0	41.0
47	35.29033581416873	37.0	34.0	40.0	28.0	41.0
48	35.269923218546786	37.0	34.0	40.0	28.0	41.0
49	35.2292144273614	37.0	34.0	40.0	28.0	41.0
50	35.11280017374838	37.0	34.0	40.0	27.0	41.0
51	34.42765097584415	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	32.0
10	46.0
11	54.0
12	39.0
13	40.0
14	55.0
15	65.0
16	95.0
17	198.0
18	390.0
19	673.0
20	1258.0
21	1903.0
22	2896.0
23	3887.0
24	5127.0
25	6403.0
26	7916.0
27	9792.0
28	11817.0
29	14578.0
30	18837.0
31	23431.0
32	29910.0
33	39898.0
34	59887.0
35	69967.0
36	64151.0
37	79592.0
38	108653.0
39	142799.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.59806775902917	17.494360984971618	22.44065371408455	16.466917541914665
2	29.596702187611346	26.74135197248416	25.356901032979547	18.305044806924954
3	31.325810861261065	25.9451471821959	26.85448715127891	15.874554805264122
4	28.629871945740536	26.418128883254997	26.456739634361863	18.495259536642596
5	24.873840790271228	30.55799632913962	24.69086557638612	19.877297304203037
6	23.646813832301582	36.376437789137036	24.694556309947803	15.282192068613575
7	79.45141504144267	7.147389444785916	9.576601884829241	3.8245936289421647
8	81.35980819541582	6.797053659007449	7.683681421557006	4.15945672401972
9	75.81064833413063	7.98845084297774	9.299371013830314	6.901529809061318
10	41.239263159239535	26.045790647397254	16.925278260017855	15.789667933345353
11	32.50414852647462	26.301302970898565	22.25200044856608	18.94254805406073
12	27.06116823073331	24.1619550667384	28.91845892000619	19.858417782522107
13	24.90606373329056	25.975524758434393	29.786207160874817	19.332204347400232
14	20.816588995652033	29.83589011266674	28.47869382100226	20.868827070678968
15	19.552654702065535	26.200659505697217	33.513138301723146	20.733547490514106
16	22.600490867563707	24.05407208570451	31.399199678622274	21.946237368109507
17	22.51645570338994	24.43137861674145	28.46918308451638	24.58298259535223
18	24.199856061391095	23.777834873741423	29.27191763418301	22.750391430684473
19	23.861728086624357	26.46341134503107	26.970745258472007	22.70411530987257
20	25.45569913140005	25.54952893464137	27.97788966693969	21.016882267018897
21	24.62698749551079	27.373035216695747	27.717267096968346	20.28271019082512
22	22.157886742743095	24.512148901226034	27.922102809641903	25.407861546388972
23	19.537749816527956	34.90454485447864	24.120647241105686	21.437058087887724
24	21.736859214129264	26.345307871057127	29.21201418945103	22.70581872536258
25	22.42049663078611	27.710311483717476	27.520522607872334	22.348669277624076
26	20.550856179210665	27.842326184193155	27.932465253872785	23.67435238272339
27	17.661295702992476	40.16341432600817	23.14018967531481	19.03510029568454
28	20.274477015956744	28.137442917837173	29.813745711296626	21.774334354909456
29	21.094103769232625	26.690249507783896	28.629020237995533	23.586626484987942
30	21.934313459679444	27.328036657501343	27.818052513460533	22.91959736935868
31	24.344788329332673	27.474530389642098	24.754885608552282	23.425795672472947
32	22.8075978008906	31.021751196294506	24.683626060553582	21.487024942261314
33	23.340482946681675	26.385338135072335	24.955462782500813	25.318716135745177
34	20.765344579660937	27.632380225049573	27.176148776308896	24.42612641898059
35	21.617194275956148	26.323021518396178	26.951439882918578	25.1083443227291
36	24.045129154381968	28.599210466920383	25.037368677312067	22.318291701385586
37	22.137019902990488	27.863193023945765	27.081751167904244	22.918035905159503
38	21.07635985787837	28.76543542848707	25.96686572969351	24.191338983941048
39	21.94595346552784	26.469941104409433	26.57668847511665	25.00741695494608
40	22.78233047112214	24.712300221302065	28.625187553143018	23.880181754432783
41	20.24083456002907	25.812138822684382	28.94401015235632	25.003016464930223
42	22.135174536209647	25.942875961542555	27.584684591329335	24.337264910918467
43	22.29117900483628	25.096136511717372	28.072855080507676	24.539829402938675
44	22.2037370096825	25.600063594178295	27.115109721250253	25.081089674888958
45	22.220771164582583	25.740453420813182	27.318525921015464	24.72024949358877
46	23.05090231338019	26.44893231336599	26.847673489318876	23.65249188393495
47	20.653628913774526	26.669950473194625	29.24054639890868	23.435874214122165
48	21.504910804906405	26.38292496312815	28.315165933961424	23.796998298004024
49	21.596753290076045	26.055727237755637	28.709364668607613	23.638154803560706
50	21.207948704481545	25.833005662436992	28.525679698268334	24.43336593481313
51	20.7264499259724	26.263543927536702	26.805939809813662	26.204066336677233
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2090.0
1	2662.5
2	3235.0
3	4619.5
4	6004.0
5	4182.5
6	2361.0
7	2169.5
8	1978.0
9	2074.5
10	2171.0
11	2151.0
12	2131.0
13	2111.0
14	2091.0
15	2183.5
16	2276.0
17	2306.5
18	2337.0
19	2334.0
20	2331.0
21	2300.0
22	2269.0
23	2602.0
24	2935.0
25	3770.5
26	5540.0
27	6474.0
28	7632.5
29	8791.0
30	10404.5
31	12018.0
32	13283.5
33	14549.0
34	16755.0
35	18961.0
36	19873.0
37	20785.0
38	21887.5
39	22990.0
40	26390.0
41	29790.0
42	33475.0
43	37160.0
44	41371.5
45	45583.0
46	51651.0
47	57719.0
48	59469.0
49	61219.0
50	59810.5
51	58402.0
52	53741.5
53	49081.0
54	45493.5
55	41906.0
56	38255.0
57	34604.0
58	33201.5
59	31799.0
60	29896.0
61	27993.0
62	25432.0
63	22871.0
64	20664.5
65	18458.0
66	15875.0
67	13292.0
68	11474.5
69	9657.0
70	8645.5
71	7634.0
72	6491.5
73	5349.0
74	4437.5
75	2863.0
76	2200.0
77	1935.5
78	1671.0
79	1088.5
80	506.0
81	424.0
82	342.0
83	273.5
84	205.0
85	127.5
86	50.0
87	52.0
88	54.0
89	30.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	704467.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.12841579478746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.21486060274478	21.617613177889407
2	9.82295233994269	5.722540801804621
3	4.126609543417498	3.606047958102087
4	2.1698876168939343	2.5282155493138796
5	1.4210284525229575	2.0696153810656046
6	0.8816800528525017	1.540916590647471
7	0.6640740632618253	1.3540397803257105
8	0.4966291433832845	1.1572816147421945
9	0.3892383993264259	1.0204108144989887
>10	4.739955579722593	34.545591994311984
>50	0.9736704102869417	18.695578736410884
>100	0.09356592531256129	4.200848534796756
>500	0.00243661263834795	0.4079623805165195
>1k	0.00341125769368713	1.533336685573919
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2693	0.38227482621613224	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1747	0.2479889050871084	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGC	1522	0.2160498646494442	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCG	1334	0.18936302197264032	No Hit
GAATCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTC	1320	0.18737570390096342	No Hit
GCTGTCTCTTATACACATCTGACGCACACCTTGTCGTATGCCGTCTTCTGC	1148	0.16296008187750455	TruSeq Adapter, Index 20 (95% over 23bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1038	0.1473454398857576	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.839025816681264E-4	0.0	0.0	0.09638492647632892	0.0
2	2.839025816681264E-4	0.0	0.0	0.4955519563017146	0.0
3	2.839025816681264E-4	0.0	0.0	0.6450266655499832	0.0
4	2.839025816681264E-4	0.0	0.0	0.8541209169485583	0.0
5	2.839025816681264E-4	0.0	0.0	1.5712588382422457	0.0
6	2.839025816681264E-4	0.0	0.0	2.022805894385401	0.0
7	2.839025816681264E-4	0.0	0.0	2.4759144147277303	0.0
8	2.839025816681264E-4	0.0	0.0	3.15784841589457	0.0
9	2.839025816681264E-4	0.0	0.0	3.436924653674338	0.0
10	2.839025816681264E-4	0.0	0.0	4.322700708478893	0.0
11	2.839025816681264E-4	0.0	0.0	5.135797702376407	0.0
12	2.839025816681264E-4	0.0	0.0	6.014334241348424	0.0
13	2.839025816681264E-4	0.0	0.0	6.2680011980688946	0.0
14	2.839025816681264E-4	0.0	0.0	6.38695637978784	0.0
15	2.839025816681264E-4	0.0	0.0	6.599883316038935	0.0
16	2.839025816681264E-4	0.0	0.0	6.966827402844988	0.0
17	2.839025816681264E-4	0.0	0.0	7.394242739546352	0.0
18	2.839025816681264E-4	0.0	0.0	7.935786914078303	0.0
19	2.839025816681264E-4	0.0	0.0	8.258016344271626	0.0
20	4.258538725021896E-4	0.0	0.0	8.517929157788796	0.0
21	4.258538725021896E-4	0.0	0.0	8.801121983002753	0.0
22	4.258538725021896E-4	0.0	0.0	9.101065060535127	0.0
23	4.258538725021896E-4	0.0	0.0	9.414635461987574	0.0
24	5.678051633362528E-4	0.0	0.0	9.66574729547303	0.0
25	5.678051633362528E-4	0.0	0.0	9.889746432409183	0.0
26	5.678051633362528E-4	0.0	0.0	10.099124586389426	0.0
27	5.678051633362528E-4	0.0	0.0	10.3336281188473	0.0
28	5.678051633362528E-4	0.0	0.0	10.596947763344486	0.0
29	5.678051633362528E-4	0.0	0.0	10.871055705945063	0.0
30	5.678051633362528E-4	0.0	0.0	11.18263878932583	0.0
31	5.678051633362528E-4	0.0	0.0	11.462140880978101	0.0
32	5.678051633362528E-4	0.0	0.0	11.746611267809564	0.0
33	5.678051633362528E-4	0.0	0.0	12.061033377007014	0.0
34	5.678051633362528E-4	0.0	0.0	12.353594987416018	0.0
35	5.678051633362528E-4	0.0	0.0	12.684199543768552	0.0
36	5.678051633362528E-4	0.0	0.0	12.971650907707529	0.0
37	5.678051633362528E-4	0.0	0.0	13.275710572674093	0.0
38	5.678051633362528E-4	0.0	0.0	13.592261951234054	0.0
39	5.678051633362528E-4	0.0	0.0	13.922156751132416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAATC	20	7.0314243E-4	45.000004	15
CGTTCCC	20	7.0314243E-4	45.000004	22
ACTCGGA	20	7.0314243E-4	45.000004	10
TCGGTTA	20	7.0314243E-4	45.000004	31
CTAACGG	45	3.8380676E-10	45.0	2
CTAACCG	25	3.8892627E-5	45.0	42
TGCGTAG	25	3.8892627E-5	45.0	1
CTTACGA	25	3.8892627E-5	45.0	29
CTATGCG	30	2.164301E-6	44.999996	1
CCCGTTA	30	2.164301E-6	44.999996	34
ACGATAG	30	2.164301E-6	44.999996	1
TTAACGG	90	0.0	42.5	2
GTTAACG	70	0.0	41.785713	1
CATACGA	130	0.0	41.53846	18
TACGAAT	60	3.6379788E-12	41.249996	12
GTCGAAT	55	6.002665E-11	40.909092	43
GCGATAC	45	1.9264917E-8	40.0	9
GCTAAGT	90	0.0	40.0	24
ATAGGGA	550	0.0	39.68182	4
ATTCGTG	40	3.4556797E-7	39.375004	3
>>END_MODULE