FastQCFastQC Report
Sat 18 Jun 2016
SRR3552669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372434
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29120.7818835015063073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26440.7099244429885564No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14050.3772480493188055No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC7800.209433080760618No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC7570.2032574899176767No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC6860.1841937094894666No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.1753330791495943No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC6220.1670094567091082No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT4950.1329094550980845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGAA302.1615342E-645.00000421
GTTACTC302.1615342E-645.00000437
ACATGCG453.8380676E-1045.0000041
AACGTTC253.88571E-545.01
TCGCAAT253.88571E-545.022
TTGTGCG406.7939254E-945.01
ATAATCG253.88571E-545.028
GAACTCG207.027139E-445.011
TTGTCGA207.027139E-445.011
TAAACCG207.027139E-445.034
GATCACG207.027139E-445.01
ATAGACG207.027139E-445.01
CTACGTA351.2089731E-745.031
CTACGAA351.2089731E-745.011
TATTCGG207.027139E-445.03
TTTGGAC207.027139E-445.023
CGAATAT351.2089731E-745.014
AGTTATC351.2089731E-745.023
ATAACGG351.2089731E-745.02
CACGTTA207.027139E-445.026