##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552669_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	372434
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.628071013924615	31.0	31.0	34.0	30.0	34.0
2	31.768850319788204	31.0	31.0	34.0	30.0	34.0
3	31.770950020674803	33.0	31.0	34.0	30.0	34.0
4	35.49598049587309	37.0	35.0	37.0	33.0	37.0
5	35.379828372275355	37.0	35.0	37.0	33.0	37.0
6	35.31317763684304	37.0	35.0	37.0	32.0	37.0
7	35.659357094142855	37.0	35.0	37.0	35.0	37.0
8	35.42373682316867	37.0	35.0	37.0	33.0	37.0
9	37.32031715686538	39.0	37.0	39.0	34.0	39.0
10	36.86880628514045	39.0	37.0	39.0	32.0	39.0
11	36.755621130186825	39.0	37.0	39.0	32.0	39.0
12	36.554283443509455	39.0	35.0	39.0	32.0	39.0
13	36.30948033745577	39.0	35.0	39.0	32.0	39.0
14	37.38805801833345	39.0	36.0	41.0	32.0	41.0
15	37.554573427775125	39.0	36.0	41.0	33.0	41.0
16	37.6160098165044	39.0	36.0	41.0	33.0	41.0
17	37.57428698776159	39.0	36.0	41.0	33.0	41.0
18	37.51730776459722	39.0	36.0	41.0	33.0	41.0
19	37.332426684996534	39.0	35.0	41.0	32.0	41.0
20	37.17410601609949	39.0	35.0	41.0	32.0	41.0
21	37.00365702379482	39.0	35.0	40.0	32.0	41.0
22	37.08096736602995	39.0	35.0	40.0	32.0	41.0
23	33.33246696058899	38.0	34.0	40.0	8.0	41.0
24	34.80030555749475	38.0	34.0	40.0	18.0	41.0
25	35.78642121825612	38.0	34.0	40.0	27.0	41.0
26	36.35055875671931	38.0	35.0	40.0	30.0	41.0
27	30.608172186212858	37.0	31.0	40.0	0.0	41.0
28	32.94744303688707	37.0	31.0	40.0	16.0	41.0
29	34.76214040608537	37.0	32.0	40.0	25.0	41.0
30	35.661856865914494	37.0	34.0	40.0	30.0	41.0
31	36.00231987412535	38.0	35.0	40.0	30.0	41.0
32	34.51514362276269	38.0	34.0	40.0	24.0	41.0
33	35.02419489090685	38.0	34.0	40.0	24.0	41.0
34	35.34613649666787	38.0	34.0	40.0	26.0	41.0
35	35.455817675078	38.0	34.0	40.0	28.0	41.0
36	35.44619986359999	38.0	34.0	40.0	28.0	41.0
37	35.41466138966904	38.0	34.0	40.0	27.0	41.0
38	35.33880902388074	38.0	34.0	40.0	27.0	41.0
39	35.30938904611287	38.0	34.0	40.0	27.0	41.0
40	35.172803771943485	38.0	34.0	40.0	27.0	41.0
41	35.075822830353836	38.0	34.0	40.0	26.0	41.0
42	34.959837179204904	37.0	34.0	40.0	26.0	41.0
43	34.88983014440142	37.0	34.0	40.0	26.0	41.0
44	34.785298871746406	37.0	34.0	40.0	25.0	41.0
45	34.72589237287681	37.0	34.0	40.0	25.0	41.0
46	34.594712083214745	37.0	34.0	40.0	24.0	41.0
47	34.50826455157156	37.0	34.0	40.0	24.0	41.0
48	34.467481486652666	36.0	34.0	40.0	24.0	41.0
49	34.46106155721551	36.0	34.0	40.0	24.0	41.0
50	34.31185122733155	36.0	34.0	40.0	24.0	41.0
51	33.57742311389401	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	26.0
11	31.0
12	22.0
13	20.0
14	33.0
15	52.0
16	79.0
17	163.0
18	269.0
19	476.0
20	784.0
21	1141.0
22	1759.0
23	2412.0
24	3173.0
25	4254.0
26	5416.0
27	6242.0
28	7332.0
29	8778.0
30	10916.0
31	13200.0
32	16610.0
33	21748.0
34	32502.0
35	39791.0
36	33224.0
37	41808.0
38	55955.0
39	64170.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.50955068549059	19.048744207027283	22.45122625753825	18.99047884994388
2	31.350521166166356	27.10037214647427	23.989485385330017	17.559621302029353
3	29.54080454523486	24.901056294538094	29.44548564309381	16.11265351713324
4	27.013108362824017	27.112186320260772	26.912419381689105	18.962285935226106
5	23.001390850459412	30.69429751311642	27.07566978310251	19.228641853321662
6	22.44961523384009	35.50427726791861	26.900605207902608	15.145502290338692
7	75.27830434385689	8.678584661980377	11.167079267736028	4.876031726426696
8	77.44459420998083	7.2071830176621905	10.08957291761762	5.258649854739363
9	72.40772861768797	9.280570517192308	12.926585650074912	5.385115215044813
10	39.85699479639345	25.80161854180875	19.06136389266286	15.280022769134934
11	31.426776287879193	26.47556345553843	23.887453884446643	18.210206372135733
12	27.491582401177123	22.709795561092704	30.476809313865004	19.32181272386517
13	22.477808148557866	27.247512310906092	31.481282589666893	18.79339695086915
14	18.86481900148751	29.780847076260493	30.2475069408271	21.1068269814249
15	17.41973074423925	25.59379648474629	36.708517482292166	20.277955288722296
16	18.48864496796748	25.652867353678772	34.88242212042939	20.976065557924358
17	19.016523733064115	24.671753921500187	29.628873840734197	26.682848504701507
18	21.66853724418286	24.26792398116176	31.744416460366132	22.319122314289245
19	23.156586133382024	26.596390232900326	29.515296670014017	20.731726963703636
20	24.727334239086655	25.083101972430015	27.95448321044803	22.2350805780353
21	22.039609702658726	28.338980866408548	28.192646213825807	21.428763217106926
22	21.298270297556076	26.012931150217224	27.51816429219674	25.170634260029967
23	17.776303989431685	35.36760875752482	25.026716142994466	21.829371110049028
24	20.1960615840659	26.516107551942085	31.542501490196923	21.745329373795087
25	19.979647400613263	27.78854776953769	28.833565141743232	23.39823968810581
26	19.574206436576684	28.725358050016915	28.883775380335848	22.816660133070556
27	17.000059070868932	39.24614831084165	25.10592480815393	18.647867810135487
28	18.360031576064483	27.897023365213702	32.06742671184693	21.675518346874885
29	20.48711986553322	26.500802826809583	29.138317124644903	23.87376018301229
30	22.260856957205842	26.109055564207353	28.920023413544417	22.710064065042395
31	23.542157805141315	27.380958773903565	26.033337450393894	23.043545970561226
32	23.403609767099674	30.608107745264935	25.95815634447983	20.030126143155567
33	23.76958065053137	26.176181551630624	26.46240676200347	23.591831035834538
34	21.57724590128721	25.525059473624857	30.06734079058303	22.830353834504905
35	22.676769575280453	24.934082280350346	28.64588088090776	23.743267263461444
36	24.034594048878457	26.486303613526157	28.73582970405495	20.74327263354044
37	22.497677440835155	27.188709945923307	30.536954198596263	19.77665841464528
38	22.396451451800857	28.52451709564648	27.513599725051957	21.565431727500712
39	24.089905862515238	24.984561022892645	27.854599741162193	23.070933373429924
40	23.28385700553655	25.58842640575243	28.67031474032983	22.45740184838119
41	20.396365530536954	26.50670991370283	27.983750140964574	25.11317441479564
42	23.23686881434026	25.824709881482356	28.065106837721583	22.873314466455803
43	23.315271967650645	24.216371222820687	29.251625791415393	23.216731018113276
44	22.188092386839013	25.336838204889993	28.320722597829413	24.15434681044158
45	23.060461719391892	25.867939017382945	26.88852253016642	24.18307673305874
46	22.33952861446592	26.335404393798633	28.313204487238007	23.01186250449744
47	21.158648243715668	26.511005976897923	30.211795915517918	22.1185498638685
48	22.290929399571468	25.02886417459201	29.307474612951555	23.372731812884968
49	22.72429477437613	23.399313703904586	29.922348657748753	23.95404286397053
50	21.854878985269874	24.429026350977622	29.916978578754893	23.79911608499761
51	21.002915952893666	24.966839762212903	27.540181616071575	26.490062668821857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3012.0
1	3102.5
2	3193.0
3	4665.5
4	6138.0
5	4089.5
6	2041.0
7	2033.5
8	2026.0
9	1986.5
10	1947.0
11	2002.5
12	2058.0
13	1979.0
14	1900.0
15	1839.5
16	1779.0
17	1694.5
18	1610.0
19	1687.5
20	1765.0
21	1730.5
22	1696.0
23	1729.0
24	1762.0
25	2076.0
26	2926.5
27	3463.0
28	3870.0
29	4277.0
30	4588.5
31	4900.0
32	5816.5
33	6733.0
34	6944.0
35	7155.0
36	8042.0
37	8929.0
38	9111.5
39	9294.0
40	11310.5
41	13327.0
42	16004.5
43	18682.0
44	22107.5
45	25533.0
46	28347.5
47	31162.0
48	32647.5
49	34133.0
50	34485.0
51	34837.0
52	30811.0
53	26785.0
54	24344.0
55	21903.0
56	20327.0
57	18751.0
58	17131.5
59	15512.0
60	14376.5
61	13241.0
62	12141.0
63	11041.0
64	9646.5
65	8252.0
66	6959.5
67	5667.0
68	5258.0
69	4849.0
70	4351.0
71	3853.0
72	3280.0
73	2707.0
74	2199.5
75	1392.5
76	1093.0
77	851.0
78	609.0
79	521.5
80	434.0
81	303.0
82	172.0
83	113.5
84	55.0
85	55.0
86	55.0
87	33.5
88	12.0
89	9.5
90	7.0
91	4.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	372434.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.0174800832289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.52057888834058	27.560784260679522
2	9.65430463846843	6.954474500669222
3	3.808062129632124	4.114704057291697
4	1.9952039071879102	2.874488679564841
5	1.1770255378041357	2.1196746932656123
6	0.7486254472382472	1.6178161281421137
7	0.5618134826336394	1.4164574144872597
8	0.4335558384716402	1.2492471021695923
9	0.3046589194955444	0.9875741908597928
>10	4.369365229829337	37.030244391250896
>50	0.3754104962876139	8.587928910301162
>100	0.04543658784433422	2.6806593255983633
>500	0.0037243104790437884	0.9384453884050314
>1k	0.0022345862874262735	1.8675009573148726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2912	0.7818835015063073	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2644	0.7099244429885564	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1405	0.3772480493188055	No Hit
CCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	780	0.209433080760618	No Hit
GCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGC	757	0.2032574899176767	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCC	686	0.1841937094894666	No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	653	0.1753330791495943	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	622	0.1670094567091082	No Hit
CTGTCTCTTATACACATCTGACGCGTTGCTAGTCGTATGCCGTCTTCTGCT	495	0.1329094550980845	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1602968579667807	0.0
2	0.0	0.0	0.0	0.8267236611050549	0.0
3	0.0	0.0	0.0	1.1140228872766718	0.0
4	0.0	0.0	0.0	1.485095345752536	0.0
5	0.0	0.0	0.0	2.44687649355322	0.0
6	0.0	0.0	0.0	3.2674245638153336	0.0
7	0.0	0.0	0.0	4.0750844444921785	0.0
8	0.0	0.0	0.0	5.460833328858268	0.0
9	0.0	0.0	0.0	6.085373515844418	0.0
10	0.0	0.0	0.0	7.412588539177412	0.0
11	0.0	0.0	0.0	9.050731136255015	0.0
12	0.0	0.0	0.0	10.328272928894783	0.0
13	0.0	0.0	0.0	10.788488698668758	0.0
14	0.0	0.0	0.0	10.984765085894413	0.0
15	0.0	0.0	0.0	11.246287932895493	0.0
16	0.0	0.0	0.0	11.85230134735282	0.0
17	0.0	0.0	0.0	12.522218701837105	0.0
18	0.0	0.0	0.0	13.424660476755614	0.0
19	0.0	0.0	0.0	13.823388842049866	0.0
20	0.0	0.0	0.0	14.209497521708544	0.0
21	0.0	0.0	0.0	14.62487313188377	0.0
22	0.0	0.0	0.0	15.024675512976795	0.0
23	0.0	0.0	0.0	15.467170022071025	0.0
24	0.0	0.0	0.0	15.78803224195428	0.0
25	0.0	0.0	0.0	16.05814721534554	0.0
26	0.0013425197484655	0.0	0.0	16.327456676887717	0.0
27	0.0013425197484655	0.0	0.0	16.62818110054399	0.0
28	0.0013425197484655	0.0	0.0	16.946358280930312	0.0
29	0.0013425197484655	0.0	0.0	17.285210265443006	0.0
30	0.0013425197484655	0.0	0.0	17.680448079391248	0.0
31	0.0013425197484655	0.0	0.0	18.035678804835218	0.0
32	0.0013425197484655	0.0	0.0	18.380169372291466	0.0
33	0.0013425197484655	0.0	0.0	18.745871751773468	0.0
34	0.0013425197484655	0.0	0.0	19.10969460360762	0.0
35	0.0016110236981586	0.0	0.0	19.51352454394604	0.0
36	0.0016110236981586	0.0	0.0	19.86660723779247	0.0
37	0.0016110236981586	0.0	0.0	20.211097805248716	0.0
38	0.0016110236981586	0.0	0.0	20.565254514893915	0.0
39	0.0016110236981586	0.0	0.0	20.92504980748267	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	30	2.1615342E-6	45.000004	21
GTTACTC	30	2.1615342E-6	45.000004	37
ACATGCG	45	3.8380676E-10	45.000004	1
AACGTTC	25	3.88571E-5	45.0	1
TCGCAAT	25	3.88571E-5	45.0	22
TTGTGCG	40	6.7939254E-9	45.0	1
ATAATCG	25	3.88571E-5	45.0	28
GAACTCG	20	7.027139E-4	45.0	11
TTGTCGA	20	7.027139E-4	45.0	11
TAAACCG	20	7.027139E-4	45.0	34
GATCACG	20	7.027139E-4	45.0	1
ATAGACG	20	7.027139E-4	45.0	1
CTACGTA	35	1.2089731E-7	45.0	31
CTACGAA	35	1.2089731E-7	45.0	11
TATTCGG	20	7.027139E-4	45.0	3
TTTGGAC	20	7.027139E-4	45.0	23
CGAATAT	35	1.2089731E-7	45.0	14
AGTTATC	35	1.2089731E-7	45.0	23
ATAACGG	35	1.2089731E-7	45.0	2
CACGTTA	20	7.027139E-4	45.0	26
>>END_MODULE