FastQCFastQC Report
Sat 18 Jun 2016
SRR3552668_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552668_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403266
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.42707512331945613No Hit
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC25860.18428437659004068No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC25790.18378554030383407No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT19190.13675240474721115Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18110.12905607347430922No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCC14470.10311658659156568No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG253.8912473E-545.00000432
ACGCCGT207.0338213E-445.018
CGTACAT207.0338213E-445.035
CGTTTTT40400.040.655941
CTAAACG501.0822987E-940.5000041
TTAACGG800.039.3752
AATTGCG356.2492763E-638.5714261
TTGCGAG1500.037.5000041
ACTAACG301.1400506E-437.4999961
CGAACGG1950.036.923082
TACGGGA5150.036.6990284
TAACGGG3600.036.253
CGCATCG504.8803486E-836.00000421
TCGATAG250.002107266636.0000041
CGTTAAT250.002107266636.00000430
GTCGTAC250.002107266636.00000411
AACGACA250.002107266636.00000437
CGGTCTA504.8803486E-836.00000431
TATCGCG250.002107266636.0000041
ACGACTC250.002107266636.00000414
Produced by FastQC (version 0.11.5)