FastQCFastQC Report
Sat 18 Jun 2016
SRR3552666_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552666_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences910892
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC24320.26699103735678875No Hit
ACCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTC18790.206281315457815No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.19563241306323909No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17290.18981394062084198No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC12190.1338248661751338No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG11960.1312998687001313No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT11660.1280063937327367Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC11460.12581074375447365No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT11380.1249324837631684No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC10690.11735749133816084No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9780.10736728393706389No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.032516E-445.016
ATAGGCG207.032516E-445.01
CCGATCG253.8901657E-545.040
TCTAGCG207.032516E-445.01
ATCGTGA207.032516E-445.030
TATTACG406.8121153E-945.01
TTACGAG453.8380676E-1045.01
CGGTCTA207.032516E-445.031
CGATCGA351.2115561E-745.041
CAATTCG302.1650048E-644.99999626
CGTTACA302.1650048E-644.99999632
TATCGGG1050.042.8571433
CGAATAT1800.042.514
GCTACGA1800.042.510
CGTTAGG1700.042.352942
GTATCGG650.041.5384642
TACGAAT1850.041.35135312
AGGGTAC2800.040.982146
ATACCCC3750.040.829
ACGTAAG1050.040.7142871