##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552666_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	910892
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614556939790887	31.0	31.0	34.0	30.0	34.0
2	31.758780404263074	31.0	31.0	34.0	30.0	34.0
3	31.79165148008765	33.0	31.0	34.0	30.0	34.0
4	35.46803682544144	37.0	35.0	37.0	33.0	37.0
5	35.263693171089436	37.0	35.0	37.0	33.0	37.0
6	35.279515024832804	37.0	35.0	37.0	32.0	37.0
7	35.665834149383244	37.0	35.0	37.0	35.0	37.0
8	35.44483209864616	37.0	35.0	37.0	33.0	37.0
9	37.0605757872503	39.0	37.0	39.0	33.0	39.0
10	36.69903786617952	39.0	37.0	39.0	32.0	39.0
11	36.64474054004207	39.0	35.0	39.0	32.0	39.0
12	36.54133640431577	39.0	35.0	39.0	32.0	39.0
13	36.35567992692877	39.0	35.0	39.0	32.0	39.0
14	37.456969651726	39.0	36.0	41.0	32.0	41.0
15	37.61734431743829	39.0	36.0	41.0	33.0	41.0
16	37.6546560953439	39.0	36.0	41.0	33.0	41.0
17	37.57942654013868	39.0	36.0	41.0	32.0	41.0
18	37.54155048018865	39.0	36.0	41.0	32.0	41.0
19	37.469274074204186	39.0	36.0	41.0	32.0	41.0
20	37.38212323744198	39.0	35.0	41.0	32.0	41.0
21	37.26944248055752	39.0	35.0	41.0	32.0	41.0
22	37.30929572331297	39.0	35.0	41.0	32.0	41.0
23	37.17866772350619	39.0	35.0	41.0	32.0	41.0
24	37.203790350557476	39.0	35.0	41.0	32.0	41.0
25	36.988866956785216	39.0	35.0	41.0	31.0	41.0
26	36.99115482406257	39.0	35.0	41.0	31.0	41.0
27	36.76366792111469	39.0	35.0	41.0	31.0	41.0
28	36.643240911106915	39.0	35.0	41.0	31.0	41.0
29	36.65299947743531	39.0	35.0	41.0	31.0	41.0
30	36.48752870812346	39.0	35.0	40.0	30.0	41.0
31	36.492372311975515	39.0	35.0	40.0	30.0	41.0
32	36.39104416330366	39.0	35.0	40.0	30.0	41.0
33	36.23723119755141	38.0	35.0	40.0	30.0	41.0
34	36.18548082538874	38.0	35.0	40.0	30.0	41.0
35	36.07680712971461	38.0	35.0	40.0	30.0	41.0
36	35.91956455869631	38.0	35.0	40.0	29.0	41.0
37	35.8880503945583	38.0	35.0	40.0	29.0	41.0
38	35.732379908924436	38.0	35.0	40.0	29.0	41.0
39	35.69647883612986	38.0	35.0	40.0	29.0	41.0
40	35.56822433394958	38.0	35.0	40.0	28.0	41.0
41	35.46513198052019	38.0	34.0	40.0	28.0	41.0
42	35.44261119869315	38.0	34.0	40.0	28.0	41.0
43	35.34897111842019	38.0	34.0	40.0	28.0	41.0
44	35.27368886761548	37.0	34.0	40.0	27.0	41.0
45	35.23904809790842	37.0	34.0	40.0	27.0	41.0
46	35.13371069237626	37.0	34.0	40.0	27.0	41.0
47	35.02947989443315	37.0	34.0	40.0	27.0	41.0
48	34.94025746191645	37.0	34.0	40.0	27.0	41.0
49	34.861206377924056	37.0	34.0	40.0	27.0	41.0
50	34.785531105773245	36.0	34.0	40.0	27.0	41.0
51	34.05465411925893	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	34.0
10	61.0
11	55.0
12	50.0
13	43.0
14	66.0
15	96.0
16	157.0
17	259.0
18	473.0
19	813.0
20	1495.0
21	2423.0
22	3918.0
23	5563.0
24	7148.0
25	7907.0
26	9293.0
27	11042.0
28	12799.0
29	15759.0
30	19250.0
31	24333.0
32	31607.0
33	42279.0
34	68093.0
35	83922.0
36	85920.0
37	118578.0
38	168280.0
39	189065.0
40	100.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23586111196497	20.00917781690914	22.98867483741212	13.766286233713767
2	29.125187179160644	25.040180394602213	25.78900681968883	20.045625606548306
3	31.044185260162564	25.050829296996792	25.707987335490923	18.196998107349717
4	27.991573095383426	26.718974367982153	24.670213373264886	20.61923916336953
5	24.55044066695064	30.698260606087217	23.56876556166922	21.18253316529292
6	24.247331187451422	37.47359730901139	22.951897700276213	15.32717380326098
7	80.72416927583073	6.603417309626168	7.90313231425899	4.769281100284117
8	81.33565779477698	6.209298138527949	7.008404948116791	5.446639118578273
9	75.71501341542137	8.054632162759141	9.452602503919236	6.777751917900256
10	41.07051110340194	27.82119065707021	15.265915168867439	15.842383070660407
11	31.09830803212675	27.369984586537154	21.266516776961485	20.265190604374613
12	26.749054772684357	21.64340009573034	29.599447574465472	22.008097557119836
13	25.82885786679431	23.946307575431554	29.19599689095963	21.028837666814507
14	20.685877140209815	28.73644735050917	27.68659731340269	22.891078195878325
15	20.042771261576565	24.940278320591243	33.24279936589629	21.774151051935906
16	21.962098690075223	24.584253676615887	29.925501596237535	23.528146037071355
17	22.273990769487494	23.439441777949526	27.245710797767465	27.04085665479552
18	24.522446129727786	24.16016388331438	27.717885325592935	23.599504661364904
19	25.575699424300574	26.234174852781667	25.187069378148013	23.003056344769742
20	26.079820659309778	24.22778990264488	24.873091431256395	24.81929800678895
21	26.312230209508918	26.113084756480458	24.9031718359586	22.671513198052022
22	22.734528352428168	25.611598301445177	24.18310842558723	27.470764920539427
23	22.02708992943181	26.990576270293293	24.98012936769672	26.002204432578175
24	22.780088089477125	24.781971957158476	26.782867782349605	25.655072171014787
25	23.420010275641896	27.060617504599886	25.420357188338468	24.09901503141975
26	22.026541016937244	25.506316884987463	26.031077229792338	26.436064868282962
27	22.35544938368105	25.24821823004264	26.476025697887344	25.920306688388962
28	22.181773470400444	26.199812930621853	27.349125911743656	24.26928768723405
29	23.382794008510338	26.068732626919548	24.553185229423466	25.995288135146648
30	23.989781445001164	25.124932483763168	24.94642614053038	25.938859930705288
31	24.779776307180214	27.76476245262885	22.21997778002222	25.235483460168712
32	25.845215459132366	26.51719413497978	22.01896602451224	25.61862438137562
33	23.547357974381157	26.83863729179749	21.93970306029694	27.674301673524415
34	23.370059238636415	25.482494082723306	25.039192352111993	26.10825432652828
35	23.66866763568019	23.74342951744005	24.87912946869662	27.708773378183142
36	24.790315427075875	27.66244516364179	24.176411693153526	23.370827716128804
37	23.492027594928928	26.51477892000369	26.089591301713043	23.903602183354337
38	24.126899786143692	27.698344040786395	24.351185431423264	23.82357074164665
39	24.753977419935623	24.326813716664546	25.747728600097485	25.171480263302342
40	25.72752862029747	23.780096872077042	27.89803840630942	22.594336101316074
41	22.33623744637125	24.46316358031468	27.410494328636105	25.790104644677964
42	23.96321408026418	24.274008334687316	26.818107964500733	24.94466962054777
43	23.61992420616275	23.382245096015776	27.115947884052115	25.88188281376936
44	22.915998823131613	25.209684572924125	26.123953223872864	25.750363380071402
45	23.49740693737567	23.16674205064925	26.342969309204605	26.99288170277047
46	24.398831035951574	25.37106484632646	26.139322773720703	24.090781344001265
47	22.95738682522187	24.17059322071113	28.112772974183546	24.759246979883454
48	23.57765794408119	23.92204564317175	26.838307944300748	25.66198846844631
49	23.365997286176626	23.35249403881031	28.131435998998782	25.15007267601428
50	22.781734826960825	23.07990409400895	28.307527127255483	25.830833951774746
51	23.001409607286043	22.81532827162825	26.30311826209913	27.880143858986578
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2068.0
1	2372.5
2	2677.0
3	3289.0
4	3901.0
5	2919.5
6	1938.0
7	1975.5
8	2013.0
9	2210.0
10	2407.0
11	2503.5
12	2600.0
13	2578.5
14	2557.0
15	2511.5
16	2466.0
17	2439.5
18	2413.0
19	2343.0
20	2273.0
21	2633.5
22	2994.0
23	3066.5
24	3139.0
25	3853.5
26	5103.0
27	5638.0
28	6403.5
29	7169.0
30	8895.5
31	10622.0
32	11887.0
33	13152.0
34	14816.5
35	16481.0
36	17835.5
37	19190.0
38	20750.5
39	22311.0
40	25031.0
41	27751.0
42	33127.5
43	38504.0
44	44775.5
45	51047.0
46	59356.0
47	67665.0
48	75704.5
49	83744.0
50	82099.0
51	80454.0
52	74065.5
53	67677.0
54	63414.5
55	59152.0
56	54725.0
57	50298.0
58	48701.5
59	47105.0
60	43903.5
61	40702.0
62	39761.5
63	38821.0
64	36271.5
65	33722.0
66	29730.5
67	25739.0
68	22119.5
69	18500.0
70	17053.5
71	15607.0
72	13523.5
73	11440.0
74	9517.0
75	6541.0
76	5488.0
77	4414.5
78	3341.0
79	2886.0
80	2431.0
81	1705.5
82	980.0
83	643.5
84	307.0
85	223.5
86	140.0
87	110.0
88	80.0
89	49.5
90	19.0
91	12.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	910892.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.171032747697904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37168241050543	19.987258013621215
2	9.59063321270338	5.019935517616387
3	3.4797434388658446	2.732054384764348
4	1.753232647794312	1.8353563615903217
5	1.0057437040327675	1.3160675707016272
6	0.6432208678192641	1.0100252637420375
7	0.4688028920967839	0.8588339088896292
8	0.38652331103814475	0.8092571384742326
9	0.3029600813205625	0.7135900388537095
>10	4.198918999305891	28.146943477875062
>50	1.641831586455283	29.09582741499503
>100	0.14530230655911458	5.546984200828417
>500	0.0071806372427469425	1.2966803777619589
>1k	0.004223904260439377	1.6311863302860583
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	2432	0.26699103735678875	No Hit
ACCCCAGGGAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTC	1879	0.206281315457815	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1782	0.19563241306323909	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1729	0.18981394062084198	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTC	1219	0.1338248661751338	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCG	1196	0.1312998687001313	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	1166	0.1280063937327367	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1146	0.12581074375447365	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1138	0.1249324837631684	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGC	1069	0.11735749133816084	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	978	0.10736728393706389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0978249891315326E-4	0.0	0.0	0.13777703613600734	0.0
2	1.0978249891315326E-4	0.0	0.0	0.7368601327050847	0.0
3	1.0978249891315326E-4	0.0	0.0	1.0219652823825438	0.0
4	1.0978249891315326E-4	0.0	0.0	1.3719518889176763	0.0
5	1.0978249891315326E-4	0.0	0.0	2.5103964026470758	0.0
6	1.0978249891315326E-4	0.0	0.0	3.388985741449041	0.0
7	1.0978249891315326E-4	0.0	0.0	4.152193673893283	0.0
8	1.0978249891315326E-4	0.0	0.0	5.5025184105250675	0.0
9	1.0978249891315326E-4	0.0	0.0	6.181303601305094	0.0
10	1.0978249891315326E-4	0.0	0.0	7.505719668193375	0.0
11	1.0978249891315326E-4	0.0	0.0	8.739126043482653	0.0
12	1.0978249891315326E-4	0.0	0.0	9.884925984639233	0.0
13	2.1956499782630653E-4	0.0	0.0	10.35237986501144	0.0
14	2.1956499782630653E-4	0.0	0.0	10.536155768192058	0.0
15	2.1956499782630653E-4	0.0	0.0	10.82521308783039	0.0
16	2.1956499782630653E-4	0.0	0.0	11.47139287643321	0.0
17	2.1956499782630653E-4	0.0	0.0	12.14293242228497	0.0
18	2.1956499782630653E-4	0.0	0.0	13.241306323911067	0.0
19	2.1956499782630653E-4	0.0	0.0	13.661114599754965	0.0
20	3.293474967394598E-4	0.0	0.0	14.189717332021798	0.0
21	8.782599913052261E-4	0.0	0.0	14.616332122798312	0.0
22	8.782599913052261E-4	0.0	0.0	15.081041440697689	0.0
23	8.782599913052261E-4	0.0	0.0	15.547836626076418	0.0
24	8.782599913052261E-4	0.0	0.0	15.926915594823535	0.0
25	9.880424902183793E-4	0.0	0.0	16.240125064222763	0.0
26	0.0017565199826104522	0.0	0.0	16.53148781633827	0.0
27	0.0017565199826104522	0.0	0.0	16.868300523003825	0.0
28	0.0017565199826104522	0.0	0.0	17.180631732411747	0.0
29	0.0018663024815236055	0.0	0.0	17.497464024275107	0.0
30	0.0018663024815236055	0.0	0.0	17.892900585360284	0.0
31	0.0018663024815236055	0.0	0.0	18.22027199711931	0.0
32	0.0018663024815236055	0.0	0.0	18.547204278882678	0.0
33	0.0018663024815236055	0.0	0.0	18.86689091571778	0.0
34	0.0018663024815236055	0.0	0.0	19.195360152465934	0.0
35	0.0018663024815236055	0.0	0.0	19.58684454359024	0.0
36	0.0019760849804367586	0.0	0.0	19.887319243115538	0.0
37	0.0019760849804367586	0.0	0.0	20.206017837460422	0.0
38	0.0019760849804367586	0.0	0.0	20.511432749436814	0.0
39	0.0019760849804367586	0.0	0.0	20.837706336206708	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.032516E-4	45.0	16
ATAGGCG	20	7.032516E-4	45.0	1
CCGATCG	25	3.8901657E-5	45.0	40
TCTAGCG	20	7.032516E-4	45.0	1
ATCGTGA	20	7.032516E-4	45.0	30
TATTACG	40	6.8121153E-9	45.0	1
TTACGAG	45	3.8380676E-10	45.0	1
CGGTCTA	20	7.032516E-4	45.0	31
CGATCGA	35	1.2115561E-7	45.0	41
CAATTCG	30	2.1650048E-6	44.999996	26
CGTTACA	30	2.1650048E-6	44.999996	32
TATCGGG	105	0.0	42.857143	3
CGAATAT	180	0.0	42.5	14
GCTACGA	180	0.0	42.5	10
CGTTAGG	170	0.0	42.35294	2
GTATCGG	65	0.0	41.538464	2
TACGAAT	185	0.0	41.351353	12
AGGGTAC	280	0.0	40.98214	6
ATACCCC	375	0.0	40.8	29
ACGTAAG	105	0.0	40.714287	1
>>END_MODULE