##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552662_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1103686
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.877711595508142	33.0	31.0	34.0	30.0	34.0
2	32.018412845682555	33.0	31.0	34.0	30.0	34.0
3	32.04905833724447	33.0	31.0	34.0	30.0	34.0
4	35.68101072225252	37.0	35.0	37.0	33.0	37.0
5	35.49974086832668	37.0	35.0	37.0	33.0	37.0
6	35.506564367039175	37.0	35.0	37.0	33.0	37.0
7	35.83567971325178	37.0	35.0	37.0	35.0	37.0
8	35.639889425071985	37.0	35.0	37.0	35.0	37.0
9	37.33779806937843	39.0	37.0	39.0	34.0	39.0
10	36.966307446139574	39.0	37.0	39.0	33.0	39.0
11	36.9171476307573	39.0	37.0	39.0	33.0	39.0
12	36.78321007967846	39.0	35.0	39.0	33.0	39.0
13	36.62407967483505	39.0	35.0	39.0	32.0	39.0
14	37.79556141873685	40.0	37.0	41.0	33.0	41.0
15	37.93610592143055	40.0	37.0	41.0	33.0	41.0
16	37.98184175571675	40.0	37.0	41.0	33.0	41.0
17	37.957914660510326	40.0	37.0	41.0	33.0	41.0
18	37.92573068789493	40.0	36.0	41.0	33.0	41.0
19	37.88314248799024	40.0	36.0	41.0	33.0	41.0
20	37.81511045714089	40.0	36.0	41.0	33.0	41.0
21	37.725341265541104	40.0	36.0	41.0	33.0	41.0
22	37.745425782333015	40.0	36.0	41.0	33.0	41.0
23	37.642520608216465	40.0	36.0	41.0	33.0	41.0
24	37.63093941573962	40.0	36.0	41.0	33.0	41.0
25	37.45775791302961	39.0	35.0	41.0	33.0	41.0
26	37.437329095413006	39.0	35.0	41.0	33.0	41.0
27	37.222343130201885	39.0	35.0	41.0	32.0	41.0
28	37.23060453788487	39.0	35.0	41.0	32.0	41.0
29	37.191308941129996	39.0	35.0	41.0	32.0	41.0
30	37.06888191025346	39.0	35.0	41.0	32.0	41.0
31	37.04228467154607	39.0	35.0	41.0	32.0	41.0
32	36.93836199788708	39.0	35.0	41.0	31.0	41.0
33	36.81818198291906	39.0	35.0	41.0	31.0	41.0
34	36.70931225004213	39.0	35.0	41.0	31.0	41.0
35	36.57752929728202	39.0	35.0	41.0	31.0	41.0
36	36.51746783052426	39.0	35.0	41.0	31.0	41.0
37	36.4481365170891	39.0	35.0	41.0	30.0	41.0
38	36.36302716533507	38.0	35.0	40.0	30.0	41.0
39	36.30574185049009	38.0	35.0	40.0	30.0	41.0
40	36.172969485886384	38.0	35.0	40.0	30.0	41.0
41	36.092236378825135	38.0	35.0	40.0	30.0	41.0
42	36.0465304443474	38.0	35.0	40.0	30.0	41.0
43	35.95645319411499	38.0	35.0	40.0	30.0	41.0
44	35.89740650873527	38.0	35.0	40.0	30.0	41.0
45	35.84233649788074	38.0	35.0	40.0	30.0	41.0
46	35.77544156580767	38.0	35.0	40.0	30.0	41.0
47	35.70504473192557	38.0	35.0	40.0	29.0	41.0
48	35.63110703587796	37.0	35.0	40.0	29.0	41.0
49	35.54557093231227	37.0	34.0	40.0	29.0	41.0
50	35.44268931562057	37.0	34.0	40.0	29.0	41.0
51	34.74242039855539	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	24.0
10	53.0
11	53.0
12	49.0
13	66.0
14	85.0
15	107.0
16	150.0
17	227.0
18	395.0
19	678.0
20	1142.0
21	1756.0
22	2665.0
23	3968.0
24	5390.0
25	6918.0
26	8676.0
27	10714.0
28	12876.0
29	15584.0
30	20143.0
31	25195.0
32	33054.0
33	45940.0
34	77384.0
35	100638.0
36	102952.0
37	140837.0
38	212987.0
39	272844.0
40	131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.908321750932785	18.831714817439018	23.243386252974123	14.016577178654074
2	30.845548462153182	24.56631686910951	26.336566740902757	18.251567927834547
3	32.7263370197683	24.718262259374495	25.940439581547654	16.61496113930955
4	29.521983607656527	26.876031769905573	24.482959827342196	19.119024795095708
5	25.086029903432678	30.411638817562242	24.162307032978582	20.3400242460265
6	25.823920934033772	35.807738795273295	22.88803155970086	15.480308710992075
7	81.3130727398916	6.548058052743262	7.339043894730929	4.799825312634209
8	82.80534499848689	5.536629077473123	6.626341187620392	5.031684736419598
9	77.27424285530486	8.320210639620326	8.583238348588276	5.822308156486537
10	41.55511621964943	27.19396639986373	15.722134737597468	15.528782642889372
11	32.44527882024416	26.78986595825262	21.67400873074407	19.090846490759148
12	28.876600772321115	22.93197521758906	27.705706151930894	20.48571785815893
13	25.447183347437587	24.232345069159162	29.212203470914734	21.108268112488517
14	21.288210596129698	28.176220410515306	27.818691185717675	22.716877807637317
15	20.866442085883122	26.09664342938118	31.769814965488376	21.267099519247324
16	23.144716885056077	24.2816344503781	30.351929806122392	22.221718858443435
17	23.436194714801132	23.14489809601644	27.47411854458605	25.94478864459638
18	24.959725864059163	23.90924592683064	27.28212553208068	23.848902677029518
19	26.150644295569574	26.22204141395288	25.390373711363555	22.236940579113988
20	26.85899793963138	24.910255271879862	25.506982964357615	22.723763824131137
21	26.073629637414992	26.279394682908002	25.70160353578826	21.94537214388875
22	24.026851840106698	25.629119151642765	24.697785420853393	25.646243587397137
23	23.126595789019703	27.585835101650286	25.13550049561198	24.152068613718033
24	24.450432459956907	25.119191509179238	26.285465250080186	24.144910780783665
25	23.791730619034762	25.484512805272512	26.115308158298646	24.608448417394076
26	22.47387390978956	26.8958743700654	26.33194586141348	24.29830585873156
27	23.594029461277934	26.163147851834673	26.446561793843536	23.796260893043854
28	22.9377739683207	26.421282864872797	27.52621669569062	23.11472647111588
29	23.903447176099	26.057139440021892	26.14221798591266	23.89719539796645
30	24.28525866958537	25.2747611186515	27.054343354903477	23.38563685685965
31	25.321151124504617	26.814873070782813	24.568038373232966	23.295937431479604
32	26.085045927917903	27.316102587148883	24.910617693800592	21.68823379113262
33	25.821655797029226	26.462327147395182	24.497275493210932	23.21874156236466
34	24.90898679515732	25.967077592721115	26.05424006465607	23.069695547465493
35	24.731309448520683	25.471465616126327	25.61906194334258	24.178162992010407
36	26.883914446681395	26.57884579490906	25.32133233546498	21.215907422944568
37	25.898851666144175	27.522320660042805	25.069720916999945	21.50910675681308
38	25.639176359942955	27.540079334158445	23.602999403815943	23.217744902082657
39	26.216786296102335	25.16793725751708	24.79201512024253	23.823261326138052
40	25.64669661479805	24.749792966477784	27.686044762731427	21.91746565599274
41	23.894930260961907	25.473458936690324	27.38469093564655	23.24691986670122
42	25.283187428308413	25.151718876564534	26.181450158831403	23.383643536295647
43	24.532792841442223	24.990350516360632	26.801010432314985	23.67584620988216
44	24.84918717823729	25.364098122110818	26.509532602569934	23.277182097081962
45	24.26704696806882	25.170293000001813	26.261092375911264	24.301567656018104
46	25.059573103219574	26.4202862045908	25.602390534989116	22.91775015720051
47	24.443637048943266	26.0800626265079	26.616990702065625	22.859309622483206
48	24.224734208823886	24.62992191619718	27.354609916226174	23.79073395875276
49	23.929269737950833	24.753145369244514	27.20402360816392	24.113561284640742
50	22.725032300853684	25.254646702051126	27.08605527296713	24.934265724128057
51	22.68253833064839	24.884704526468578	26.116304818580648	26.316452324302382
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2225.0
1	2815.5
2	3406.0
3	4189.5
4	4973.0
5	3705.0
6	2437.0
7	2563.0
8	2689.0
9	2729.0
10	2769.0
11	2957.0
12	3145.0
13	3004.5
14	2864.0
15	3050.5
16	3237.0
17	3110.5
18	2984.0
19	2901.5
20	2819.0
21	3067.5
22	3316.0
23	3856.0
24	4396.0
25	5001.0
26	6919.0
27	8232.0
28	9244.5
29	10257.0
30	11827.0
31	13397.0
32	15030.0
33	16663.0
34	19255.0
35	21847.0
36	23793.5
37	25740.0
38	28155.5
39	30571.0
40	34904.0
41	39237.0
42	46138.0
43	53039.0
44	58851.5
45	64664.0
46	71928.5
47	79193.0
48	85316.5
49	91440.0
50	92312.5
51	93185.0
52	85576.5
53	77968.0
54	72657.5
55	67347.0
56	64116.0
57	60885.0
58	59619.0
59	58353.0
60	58665.0
61	58977.0
62	54692.0
63	50407.0
64	45673.0
65	40939.0
66	33399.5
67	25860.0
68	23211.5
69	20563.0
70	18216.5
71	15870.0
72	13600.0
73	11330.0
74	9958.5
75	6767.0
76	4947.0
77	4212.5
78	3478.0
79	2868.5
80	2259.0
81	1635.5
82	1012.0
83	609.0
84	206.0
85	219.5
86	233.0
87	173.0
88	113.0
89	64.5
90	16.0
91	10.0
92	4.0
93	2.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1103686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.06676224072657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.46792309026084	18.40335324055473
2	9.276497125313895	4.465105014834261
3	3.2774856645898875	2.366354047112236
4	1.63339688267224	1.57242297680067
5	0.979621649224137	1.1788160658872873
6	0.6866371035554655	0.991507915015832
7	0.46999311657694054	0.791784881400472
8	0.3880879430270675	0.7472016202660058
9	0.28719368960710573	0.6220640020330115
>10	4.280410392020608	26.47548017452538
>50	2.029678409181061	33.86612550874564
>100	0.2173842468797382	7.044398765475685
>500	0.0026556539758432218	0.44054312915142846
>1k	0.0030350331152493963	1.0348426581973658
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2197	0.1990602399595537	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1906	0.17269404522663148	No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1611	0.1459654285729818	Illumina Single End Adapter 1 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	1440	0.1304718914618832	Illumina Single End Adapter 1 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	1143	0.10356206384786977	Illumina Single End Adapter 1 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.060548018186332E-5	0.0	0.0	0.11071989678223698	0.0
2	9.060548018186332E-5	0.0	0.0	0.5137330726311651	0.0
3	1.8121096036372665E-4	0.0	0.0	0.7030079207310775	0.0
4	1.8121096036372665E-4	0.0	0.0	0.9358640047984662	0.0
5	1.8121096036372665E-4	0.0	0.0	1.586954985385336	0.0
6	1.8121096036372665E-4	0.0	0.0	2.1997198478552775	0.0
7	1.8121096036372665E-4	0.0	0.0	2.7467957371933682	0.0
8	1.8121096036372665E-4	0.0	0.0	3.7040426353147544	0.0
9	1.8121096036372665E-4	0.0	0.0	4.227289283365015	0.0
10	1.8121096036372665E-4	0.0	0.0	5.157807564832751	0.0
11	1.8121096036372665E-4	0.0	0.0	6.163890816772161	0.0
12	1.8121096036372665E-4	0.0	0.0	7.00942115782931	0.0
13	1.8121096036372665E-4	0.0	0.0	7.361967081216941	0.0
14	1.8121096036372665E-4	0.0	0.0	7.516721241367563	0.0
15	1.8121096036372665E-4	0.0	0.0	7.74658734458895	0.0
16	1.8121096036372665E-4	0.0	0.0	8.217735841534639	0.0
17	1.8121096036372665E-4	0.0	0.0	8.754845128052725	0.0
18	1.8121096036372665E-4	0.0	0.0	9.44707099664216	0.0
19	1.8121096036372665E-4	0.0	0.0	9.773522541737414	0.0
20	1.8121096036372665E-4	0.0	0.0	10.135491435063958	0.0
21	1.8121096036372665E-4	0.0	0.0	10.510688728497055	0.0
22	1.8121096036372665E-4	0.0	0.0	10.891412956221243	0.0
23	1.8121096036372665E-4	0.0	0.0	11.29723490195581	0.0
24	1.8121096036372665E-4	0.0	0.0	11.600763260565053	0.0
25	1.8121096036372665E-4	0.0	0.0	11.885989312177557	0.0
26	1.8121096036372665E-4	0.0	0.0	12.149560654026597	0.0
27	1.8121096036372665E-4	0.0	0.0	12.435511549480559	0.0
28	1.8121096036372665E-4	0.0	0.0	12.717657014766882	0.0
29	1.8121096036372665E-4	0.0	0.0	13.00496699242357	0.0
30	1.8121096036372665E-4	0.0	0.0	13.349449028075014	0.0
31	1.8121096036372665E-4	0.0	0.0	13.663487622385352	0.0
32	1.8121096036372665E-4	0.0	0.0	13.960764202862046	0.0
33	1.8121096036372665E-4	0.0	0.0	14.254416564131464	0.0
34	1.8121096036372665E-4	0.0	0.0	14.544897733594519	0.0
35	2.7181644054558995E-4	0.0	0.0	14.863557207394132	0.0
36	2.7181644054558995E-4	0.0	0.0	15.169441308488103	0.0
37	3.624219207274533E-4	0.0	0.0	15.479765078110985	0.0
38	4.530274009093166E-4	0.0	0.0	15.777857107909314	0.0
39	4.530274009093166E-4	0.0	0.0	16.0736840007031	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.8907056E-5	45.0	18
ACGATAG	20	7.033164E-4	45.0	1
TCGGAAA	25	3.8907056E-5	45.0	33
ACCTAAC	20	7.033164E-4	45.0	36
ACCGTTC	20	7.033164E-4	45.0	23
ATCGATA	20	7.033164E-4	45.0	17
CGAATAT	60	3.6379788E-12	41.250004	14
TTAACGG	100	0.0	40.5	2
ACGTACG	45	1.9281288E-8	40.0	1
CGCTATT	40	3.4580626E-7	39.375	42
TCGTATA	40	3.4580626E-7	39.375	29
TTACGCG	35	6.248065E-6	38.571426	1
TCGACGT	35	6.248065E-6	38.571426	26
GTTAACG	65	9.094947E-12	38.07692	1
ATCTCCG	30	1.1398924E-4	37.500004	35
ACTTATC	30	1.1398924E-4	37.500004	40
GTCGTTA	30	1.1398924E-4	37.500004	31
GAACGTT	30	1.1398924E-4	37.500004	34
TCGTAAG	30	1.1398924E-4	37.500004	1
TAAATCG	30	1.1398924E-4	37.500004	29
>>END_MODULE