FastQCFastQC Report
Sat 18 Jun 2016
SRR3552660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562878
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102120.6534099270704431No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT55450.042.5653761
TCGTTAG800.042.1875041
CGTATGG2700.040.02
TAACGCG750.039.01
CTATACG356.249733E-638.5714261
GTATGCG1200.037.4999961
ACGGTAT301.1401098E-437.49999626
GCGTATG2050.037.3170741
CGAATAT1400.036.96428314
ATAACGC552.750312E-936.8181811
GCGTACG1350.036.6666641
GGCGATA6150.036.5853658
CTTAACG1000.036.01
CTATGCG1000.036.01
CTCGATT250.002107340136.010
TATCGCG458.702591E-735.01
AACGGGC2600.034.6153834
TACGAAT1500.034.512
TAGCGCG850.034.4117621
ACGGGTA1900.034.3421065
Produced by FastQC (version 0.11.5)