##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552660_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1562878
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.944451198366092	33.0	31.0	34.0	30.0	34.0
2	32.107045463561455	33.0	31.0	34.0	30.0	34.0
3	32.13573164380073	33.0	31.0	34.0	30.0	34.0
4	35.7546468758278	37.0	35.0	37.0	33.0	37.0
5	35.56529172462598	37.0	35.0	37.0	33.0	37.0
6	35.57220525210541	37.0	35.0	37.0	33.0	37.0
7	35.89069524300681	37.0	35.0	37.0	35.0	37.0
8	35.73267395151765	37.0	35.0	37.0	35.0	37.0
9	37.43131837545861	39.0	37.0	39.0	35.0	39.0
10	37.03435520878789	39.0	37.0	39.0	33.0	39.0
11	36.979802006298634	39.0	37.0	39.0	33.0	39.0
12	36.9302965426604	39.0	37.0	39.0	33.0	39.0
13	36.82665313607332	39.0	37.0	39.0	33.0	39.0
14	38.03111759203214	40.0	37.0	41.0	33.0	41.0
15	38.14815103930057	40.0	37.0	41.0	33.0	41.0
16	38.1595409238597	40.0	37.0	41.0	33.0	41.0
17	38.12046941603887	40.0	37.0	41.0	33.0	41.0
18	38.05282626027112	40.0	37.0	41.0	33.0	41.0
19	37.996985689222065	40.0	37.0	41.0	33.0	41.0
20	37.904754561776414	40.0	36.0	41.0	33.0	41.0
21	37.83194465594883	40.0	36.0	41.0	33.0	41.0
22	37.854565103610135	40.0	36.0	41.0	33.0	41.0
23	37.75942332031035	40.0	36.0	41.0	33.0	41.0
24	37.76076507571288	40.0	36.0	41.0	33.0	41.0
25	37.58955273540225	40.0	36.0	41.0	33.0	41.0
26	37.58823465427244	40.0	36.0	41.0	33.0	41.0
27	37.38160112305631	39.0	35.0	41.0	32.0	41.0
28	37.37775373381672	39.0	35.0	41.0	32.0	41.0
29	37.3501380146115	39.0	35.0	41.0	32.0	41.0
30	37.216009822903644	39.0	35.0	41.0	32.0	41.0
31	37.15914997843721	39.0	35.0	41.0	32.0	41.0
32	36.999802287830526	39.0	35.0	41.0	31.0	41.0
33	36.83522194310753	39.0	35.0	41.0	31.0	41.0
34	36.7135572962189	39.0	35.0	41.0	31.0	41.0
35	36.570136632545854	39.0	35.0	41.0	30.0	41.0
36	36.50524929009174	39.0	35.0	41.0	30.0	41.0
37	36.471770669239696	39.0	35.0	41.0	30.0	41.0
38	36.36869032643623	39.0	35.0	41.0	30.0	41.0
39	36.32102377792764	39.0	35.0	41.0	30.0	41.0
40	36.26102485286759	39.0	35.0	41.0	30.0	41.0
41	36.185516719795146	39.0	35.0	41.0	30.0	41.0
42	36.12885074842694	39.0	35.0	41.0	30.0	41.0
43	36.0726633812748	39.0	35.0	40.0	30.0	41.0
44	35.99973830330966	38.0	35.0	40.0	30.0	41.0
45	35.94892947498141	38.0	35.0	40.0	29.0	41.0
46	35.857469361012186	38.0	35.0	40.0	29.0	41.0
47	35.777094565282766	38.0	35.0	40.0	29.0	41.0
48	35.70335688390264	38.0	35.0	40.0	29.0	41.0
49	35.62478133289994	38.0	35.0	40.0	29.0	41.0
50	35.54758848739313	38.0	35.0	40.0	28.0	41.0
51	34.89210418215625	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	33.0
10	63.0
11	83.0
12	50.0
13	54.0
14	73.0
15	86.0
16	160.0
17	272.0
18	462.0
19	889.0
20	1452.0
21	2316.0
22	3636.0
23	5385.0
24	8329.0
25	11880.0
26	15418.0
27	18351.0
28	19852.0
29	22697.0
30	26884.0
31	33766.0
32	43673.0
33	59526.0
34	96238.0
35	123207.0
36	136793.0
37	192178.0
38	303404.0
39	435435.0
40	218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.298872976649484	18.614632748045594	23.124389747632254	16.96210452767267
2	30.406915958891222	24.1770630848985	27.11190508792113	18.304115868289145
3	32.630825950586036	23.669217942795278	28.011079559632933	15.688876546985753
4	28.364722006452197	26.060511441072176	26.561126332317684	19.01364022015794
5	25.157433913587624	30.016802335179072	25.88525783842373	18.940505912809574
6	25.252067019946534	34.66169464283201	24.68209290808368	15.404145429137783
7	82.37706334083659	5.499789490926355	8.330080786856044	3.793066381381016
8	84.59483081852838	4.417811243104068	7.464242250514755	3.5231156878527945
9	78.38372540914902	7.375495720075399	9.54943380097487	4.691345069800714
10	38.11372352800411	32.555452185007404	15.674160107186868	13.656664179801622
11	28.542151082810047	26.7942859263487	26.885015976934863	17.778547013906394
12	25.656833098936705	24.132209935772337	29.941876461246498	20.269080504044464
13	23.877359589168186	24.314437851195038	31.816047061894785	19.992155497741987
14	21.48971320858058	26.592606716583123	30.425791392546316	21.491888682289982
15	21.398151359223178	25.403070489187257	32.03045919131244	21.16831896027713
16	24.500568822390488	24.565833033672494	30.419904816626765	20.513693327310257
17	24.539919302722286	24.19984157432634	28.921259368933466	22.338979754017906
18	25.334031191174233	23.89828252749095	29.855113450953947	20.912572830380874
19	24.88102078345207	25.77642016843285	28.267145612133515	21.07541343598157
20	25.781027053935112	25.568150553018214	27.89616336015991	20.754659032886764
21	25.348811615493982	25.619402154230848	28.021828959138205	21.009957271136965
22	23.895275255010308	24.56320966831704	28.324091835703108	23.217423240969545
23	22.928789067348827	26.278442719137384	28.468952790940815	22.32381542257297
24	21.929030928837694	25.66073615470945	29.86656668018873	22.543666236264123
25	23.0737140071074	26.676426438915897	27.930715001426854	22.319144552549847
26	22.439179513692046	26.934348042521556	28.03590555372844	22.590566890057957
27	22.75833430376523	25.512164097261593	29.072198853653326	22.657302745319853
28	21.618258110997786	26.935051872251066	29.15537873077745	22.2913112859737
29	22.600036599145934	25.869325692728417	28.262794664714715	23.267843043410938
30	23.804417235382417	25.74935471610708	28.084661758627355	22.36156628988315
31	23.906920437807685	26.541739022495676	26.913745026803117	22.637595512893522
32	23.919141481292847	26.47020432816893	27.488070086084775	22.12258410445345
33	23.722581033196448	26.75832662562273	26.574627066220142	22.94446527496068
34	22.938514714520263	26.989054807860885	27.652638273748813	22.41979220387004
35	23.214991829176686	26.129742692647795	27.948374729185517	22.706890748990006
36	23.909607787684003	28.22875489961468	26.292647282769355	21.56899002993196
37	23.52397308043238	28.09790655444635	26.264430109068016	22.113690256053257
38	22.72205508043494	28.656363452553556	26.525039062549986	22.096542404461513
39	23.651430245994888	27.587182108904212	26.060383472030445	22.70100417307045
40	24.39032349294059	26.270892545675352	27.329324489819424	22.00945947156464
41	21.950913634973425	26.469052606793365	27.540857315798164	24.039176442435046
42	22.53438848073874	27.29515675567767	26.529070087364463	23.64138467621913
43	22.806834570580687	26.56215008465152	27.618022647961006	23.01299269680679
44	22.71648842711971	26.762357650437206	27.161557076112146	23.359596846330934
45	23.37770446573565	26.00030200693848	26.931916630728693	23.690076896597176
46	23.24698408960904	26.497653687619895	26.765172969355255	23.490189253415814
47	22.396629807317016	26.879513308140496	27.916894344920077	22.806962539622415
48	22.766524322435917	26.395470407798943	27.74016909829174	23.097836171473396
49	22.77509824823179	26.860125998318484	27.50944091605359	22.85533483739614
50	22.109851184801375	26.210299204416465	28.145511037969694	23.534338572812466
51	22.57284317777843	25.920257371336724	27.08867870684724	24.4182207440376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1411.0
1	1689.0
2	1967.0
3	9438.5
4	16910.0
5	11367.0
6	5824.0
7	5719.5
8	5615.0
9	5553.0
10	5491.0
11	5507.5
12	5524.0
13	5515.5
14	5507.0
15	5388.0
16	5269.0
17	5194.0
18	5119.0
19	5058.5
20	4998.0
21	5530.0
22	6062.0
23	6951.5
24	7841.0
25	8767.0
26	12125.5
27	14558.0
28	17085.0
29	19612.0
30	22840.5
31	26069.0
32	30106.5
33	34144.0
34	37906.5
35	41669.0
36	46549.0
37	51429.0
38	54122.5
39	56816.0
40	62777.5
41	68739.0
42	75067.0
43	81395.0
44	87363.0
45	93331.0
46	101156.5
47	108982.0
48	115298.0
49	121614.0
50	120548.0
51	119482.0
52	115187.5
53	110893.0
54	103063.0
55	95233.0
56	88634.0
57	82035.0
58	77276.0
59	72517.0
60	69640.5
61	66764.0
62	60773.0
63	54782.0
64	49312.0
65	43842.0
66	38339.5
67	32837.0
68	28248.0
69	23659.0
70	20758.5
71	17858.0
72	15924.5
73	13991.0
74	12230.0
75	8212.5
76	5956.0
77	4722.5
78	3489.0
79	2613.0
80	1737.0
81	1430.5
82	1124.0
83	762.5
84	401.0
85	271.5
86	142.0
87	100.5
88	59.0
89	33.5
90	8.0
91	6.5
92	5.0
93	4.5
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1562878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.610706502205804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12346850848189	19.49575809900636
2	7.592109112091102	3.888785564049751
3	2.758174998106091	2.1191643107465143
4	1.4727089400159177	1.5086846570368913
5	1.0112689325789241	1.294965591353889
6	0.7674223903538538	1.179253776154426
7	0.6284264360385236	1.1266111508126884
8	0.5075623821822696	1.0399224961304414
9	0.44144363365806955	1.0175115004995672
>10	7.765013169158527	50.268209128477615
>50	0.8862538337578583	13.789214946276346
>100	0.042869414294752904	1.9790904308226327
>500	0.0020173841736964826	0.3387393689968602
>1k	0.0010086920868482413	0.29456439694846126
>5k	0.0	0.0
>10k+	2.5217302171206033E-4	0.6595245826874998
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10212	0.6534099270704431	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2796904172942481E-4	0.0	0.0	0.07441399776566053	0.0
2	1.2796904172942481E-4	0.0	0.0	0.25900934046035584	0.0
3	1.2796904172942481E-4	0.0	0.0	0.3780845337895856	0.0
4	1.2796904172942481E-4	0.0	0.0	0.5052857612686339	0.0
5	1.2796904172942481E-4	0.0	0.0	0.8759480906379129	0.0
6	1.2796904172942481E-4	0.0	0.0	1.3370845325098952	0.0
7	1.2796904172942481E-4	0.0	0.0	1.7136334377987277	0.0
8	1.2796904172942481E-4	0.0	0.0	2.422006068291959	0.0
9	1.2796904172942481E-4	0.0	0.0	2.8173664227150166	0.0
10	1.2796904172942481E-4	0.0	0.0	3.4607307800097002	0.0
11	1.2796904172942481E-4	0.0	0.0	4.10499092059649	0.0
12	1.2796904172942481E-4	0.0	0.0	4.685074586755972	0.0
13	1.2796904172942481E-4	0.0	0.0	4.96014404195337	0.0
14	1.2796904172942481E-4	0.0	0.0	5.095791226186561	0.0
15	1.2796904172942481E-4	0.0	0.0	5.270980844314144	0.0
16	1.2796904172942481E-4	0.0	0.0	5.568956757981109	0.0
17	1.2796904172942481E-4	0.0	0.0	5.953247790294572	0.0
18	1.2796904172942481E-4	0.0	0.0	6.393141371239469	0.0
19	1.2796904172942481E-4	0.0	0.0	6.639865683693801	0.0
20	1.2796904172942481E-4	0.0	0.0	6.86809846961823	0.0
21	1.2796904172942481E-4	0.0	0.0	7.134721968061487	0.0
22	1.2796904172942481E-4	0.0	0.0	7.432697881728452	0.0
23	1.2796904172942481E-4	0.0	0.0	7.74590211136122	0.0
24	1.2796904172942481E-4	0.0	0.0	7.991154779835663	0.0
25	1.2796904172942481E-4	0.0	0.0	8.216700215883773	0.0
26	1.2796904172942481E-4	0.0	0.0	8.427209289528678	0.0
27	1.2796904172942481E-4	0.0	0.0	8.65806544080856	0.0
28	1.2796904172942481E-4	0.0	0.0	8.888857607567576	0.0
29	1.2796904172942481E-4	0.0	0.0	9.133086523708185	0.0
30	1.2796904172942481E-4	0.0	0.0	9.42767125776932	0.0
31	1.2796904172942481E-4	0.0	0.0	9.678490579558993	0.0
32	1.2796904172942481E-4	0.0	0.0	9.94293860429285	0.0
33	1.2796904172942481E-4	0.0	0.0	10.199324579397752	0.0
34	1.2796904172942481E-4	0.0	0.0	10.436259260159781	0.0
35	1.2796904172942481E-4	0.0	0.0	10.697252120766944	0.0
36	1.2796904172942481E-4	0.0	0.0	10.943592526096086	0.0
37	1.2796904172942481E-4	0.0	0.0	11.192748250343277	0.0
38	1.2796904172942481E-4	0.0	0.0	11.459691671390857	0.0
39	1.2796904172942481E-4	0.0	0.0	11.755236173264963	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	5545	0.0	42.565376	1
TCGTTAG	80	0.0	42.187504	1
CGTATGG	270	0.0	40.0	2
TAACGCG	75	0.0	39.0	1
CTATACG	35	6.249733E-6	38.571426	1
GTATGCG	120	0.0	37.499996	1
ACGGTAT	30	1.1401098E-4	37.499996	26
GCGTATG	205	0.0	37.317074	1
CGAATAT	140	0.0	36.964283	14
ATAACGC	55	2.750312E-9	36.81818	11
GCGTACG	135	0.0	36.666664	1
GGCGATA	615	0.0	36.585365	8
CTTAACG	100	0.0	36.0	1
CTATGCG	100	0.0	36.0	1
CTCGATT	25	0.0021073401	36.0	10
TATCGCG	45	8.702591E-7	35.0	1
AACGGGC	260	0.0	34.615383	4
TACGAAT	150	0.0	34.5	12
TAGCGCG	85	0.0	34.411762	1
ACGGGTA	190	0.0	34.342106	5
>>END_MODULE