FastQCFastQC Report
Sat 18 Jun 2016
SRR3552659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142723
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22280.1949728849423701No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16360.14316680420364342No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC16300.14264174257453469Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC16150.1413290885017629Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT12330.10790016478184127Illumina Single End Adapter 1 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC11480.10046179170280112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA253.8907907E-545.00000417
TCAATCG253.8907907E-545.00000419
AGCGTAA207.033269E-445.044
CACGGAT207.033269E-445.045
ATTACGA207.033269E-445.019
ACCGAAT207.033269E-445.023
GTATCGT207.033269E-445.029
ACGCATT1600.042.187517
ACCGCTA1600.042.187526
TACGCAT1600.042.187516
GCAAACG650.041.5384641
AAATGCG650.041.5384641
CGATCTA501.0822987E-940.50000410
GCTACGA1350.040.010
TCTAGCG403.45819E-739.3751
CTATGCG356.2482577E-638.5714261
TAACGAG356.2482577E-638.5714261
CGGATAC356.2482577E-638.57142623
TATTACG356.2482577E-638.5714261
TATAGCG356.2482577E-638.5714261