##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552659_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1142723
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780758766560226	31.0	31.0	34.0	30.0	34.0
2	31.918806219880057	33.0	31.0	34.0	30.0	34.0
3	31.961141939035095	33.0	31.0	34.0	30.0	34.0
4	35.595398009841404	37.0	35.0	37.0	33.0	37.0
5	35.409631205462745	37.0	35.0	37.0	33.0	37.0
6	35.41878302965811	37.0	35.0	37.0	33.0	37.0
7	35.77257130555699	37.0	35.0	37.0	35.0	37.0
8	35.57822061864511	37.0	35.0	37.0	35.0	37.0
9	37.25326085149244	39.0	37.0	39.0	34.0	39.0
10	36.865266560662555	39.0	37.0	39.0	32.0	39.0
11	36.8043279079882	39.0	37.0	39.0	32.0	39.0
12	36.65891121470383	39.0	35.0	39.0	32.0	39.0
13	36.50059113188411	39.0	35.0	39.0	32.0	39.0
14	37.636724735565835	40.0	36.0	41.0	33.0	41.0
15	37.785295299035724	40.0	36.0	41.0	33.0	41.0
16	37.81330471164053	40.0	36.0	41.0	33.0	41.0
17	37.79278005255867	40.0	36.0	41.0	33.0	41.0
18	37.76839531540015	40.0	36.0	41.0	33.0	41.0
19	37.739219390876	40.0	36.0	41.0	33.0	41.0
20	37.67461055741418	40.0	36.0	41.0	33.0	41.0
21	37.58659097611582	39.0	36.0	41.0	33.0	41.0
22	37.61542823588919	39.0	36.0	41.0	33.0	41.0
23	37.519702500080946	39.0	35.0	41.0	33.0	41.0
24	37.52951327662085	39.0	35.0	41.0	33.0	41.0
25	37.34897258565724	39.0	35.0	41.0	32.0	41.0
26	37.33823245003382	39.0	35.0	41.0	32.0	41.0
27	37.10587255179077	39.0	35.0	41.0	32.0	41.0
28	37.11013517711641	39.0	35.0	41.0	32.0	41.0
29	37.071637658470166	39.0	35.0	41.0	32.0	41.0
30	36.92521109665247	39.0	35.0	41.0	31.0	41.0
31	36.878595250117485	39.0	35.0	41.0	31.0	41.0
32	36.78238733271318	39.0	35.0	41.0	31.0	41.0
33	36.65160498213478	39.0	35.0	41.0	31.0	41.0
34	36.57128980514088	39.0	35.0	41.0	30.0	41.0
35	36.43395643563663	39.0	35.0	41.0	30.0	41.0
36	36.37918288159073	38.0	35.0	40.0	30.0	41.0
37	36.341809869933485	38.0	35.0	40.0	30.0	41.0
38	36.27075853028249	38.0	35.0	40.0	30.0	41.0
39	36.224903148006995	38.0	35.0	40.0	30.0	41.0
40	36.11335205469742	38.0	35.0	40.0	30.0	41.0
41	36.02316921948714	38.0	35.0	40.0	30.0	41.0
42	35.98937800324313	38.0	35.0	40.0	30.0	41.0
43	35.8841530274616	38.0	35.0	40.0	29.0	41.0
44	35.803087012338075	38.0	35.0	40.0	29.0	41.0
45	35.73612415257241	38.0	35.0	40.0	29.0	41.0
46	35.63323482593769	38.0	34.0	40.0	29.0	41.0
47	35.54586019534043	37.0	34.0	40.0	29.0	41.0
48	35.46332663296354	37.0	34.0	40.0	29.0	41.0
49	35.391669722233644	37.0	34.0	40.0	29.0	41.0
50	35.28004599539871	37.0	34.0	40.0	28.0	41.0
51	34.55035997350189	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	29.0
10	56.0
11	55.0
12	54.0
13	55.0
14	64.0
15	103.0
16	153.0
17	274.0
18	440.0
19	737.0
20	1320.0
21	1929.0
22	2886.0
23	4127.0
24	5726.0
25	7373.0
26	9660.0
27	11989.0
28	14786.0
29	18035.0
30	22329.0
31	28530.0
32	36382.0
33	50226.0
34	82941.0
35	104172.0
36	104923.0
37	146726.0
38	215192.0
39	271292.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.6688305039804	18.621135655797598	23.187071582527	13.522962257694997
2	30.32038385505499	24.875319740654557	26.638126650115556	18.166169754174895
3	33.016050258899135	24.522740856708054	25.60900585706247	16.852203027330333
4	28.427624192389583	27.268813176946644	25.26150256886402	19.042060061799752
5	25.513007089207097	30.751371942281725	23.885666080056147	19.84995488845503
6	25.5424980507087	35.52444468169451	23.133690316900946	15.799366950695838
7	81.41868151774314	6.712300356254315	7.278141771890476	4.590876354112064
8	82.76992762025442	5.6585016666331205	6.530804053125736	5.040766659986716
9	76.43383392125651	8.155257223316587	9.258061664987927	6.152847190438978
10	41.62802358926879	26.955088853554187	15.67597746785529	15.740910089321734
11	32.47751204797663	25.86646107586878	22.22699639370171	19.429030482452877
12	28.578141859400745	22.788637316305003	28.312635695614773	20.32058512867948
13	25.86637356559726	23.977814395964728	28.63913651864888	21.516675519789136
14	21.77894380352894	26.760028458340297	28.31062295936986	23.150404778760908
15	20.71280616562369	25.078343570576596	32.349134479659554	21.859715784140164
16	23.623397796316343	23.43507569200935	30.773074489618217	22.16845202205609
17	23.930383828801904	22.978884646585392	26.722399041587508	26.3683324830252
18	24.914174301208604	24.20114060887897	27.452934788220766	23.43175030169166
19	25.735370689134633	26.499947931388444	25.296856718557343	22.467824660919575
20	27.109106931426076	24.851866987887703	25.0556784102534	22.983347670432817
21	25.731520237187837	26.779368228345803	25.120348500905294	22.368763033561066
22	23.98875317990449	25.09252023456253	25.14843929806261	25.770287287470367
23	23.64667552854016	26.769479567664256	25.078343570576596	24.505501333218987
24	22.571874373754618	24.82412623181646	27.757820574189896	24.846178820239025
25	23.800606096140534	25.702991888672933	25.730295093386584	24.766106921799945
26	22.94081767847501	26.102126237067075	25.9037404515355	25.053315632922413
27	23.08013403073186	26.486558859846177	25.9530087344002	24.480298375021768
28	22.150512416394875	26.824523528449152	26.64539000265156	24.379574052504413
29	24.10706706699699	26.505898629851675	25.3444623062632	24.042571996888135
30	24.403726887443415	25.41368293103403	25.583715388593735	24.59887479292882
31	25.838282768439946	27.1106821163134	23.04688012755497	24.00415498769168
32	26.117265514039712	27.147086389264942	23.332338633247076	23.40330946344827
33	24.920650061300943	26.866353438234814	23.302672651202435	24.910323849261808
34	24.60027495727311	27.084866586215554	23.972826310488195	24.34203214602314
35	23.796405603107665	26.597696904674184	24.60298777569017	25.002909716527977
36	24.39768867870866	29.265710062718615	23.337151698180573	22.99944956039215
37	23.86151324511715	29.73310242289689	23.957336992429486	22.44804733955648
38	23.83421004040349	28.019301265486035	23.70635753371552	24.44013116039495
39	23.91576961345838	26.784706354908405	23.92688341794118	25.37264061369203
40	25.17162952001491	25.028462715811266	26.451904792325	23.34800297184882
41	22.4916274547725	26.797832895636127	25.7482346990478	24.962304950543572
42	24.279462301887683	26.57135631294723	24.759193610350014	24.38998777481507
43	23.822046112662473	25.952746203585647	25.607955733804253	24.617251949947626
44	22.866871499042198	25.781751133039236	25.692578166362278	25.65879920155628
45	23.27501940540271	25.136537901136148	25.878099941980693	25.710342751480454
46	24.13682055931315	26.454792631285095	25.229298788945353	24.1790880204564
47	23.06411965104404	26.324314816451583	26.179747847903645	24.43181768460073
48	22.910014062900633	25.54661103347005	26.67619361822594	24.867181285403376
49	23.27886985734951	25.08088136845062	26.717235935567935	24.923012838631934
50	22.129597461502044	24.690148006122218	27.16625113872741	26.014003393648327
51	21.940662785294425	25.026187448751795	25.73729591510804	27.29585385084574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2099.0
1	2589.0
2	3079.0
3	3924.5
4	4770.0
5	3554.0
6	2338.0
7	2272.0
8	2206.0
9	2303.0
10	2400.0
11	2417.5
12	2435.0
13	2556.0
14	2677.0
15	2739.5
16	2802.0
17	2602.5
18	2403.0
19	2555.0
20	2707.0
21	2642.0
22	2577.0
23	3191.5
24	3806.0
25	4693.0
26	6219.0
27	6858.0
28	8878.0
29	10898.0
30	13147.5
31	15397.0
32	17288.0
33	19179.0
34	21158.0
35	23137.0
36	26142.0
37	29147.0
38	30294.0
39	31441.0
40	34217.5
41	36994.0
42	44213.5
43	51433.0
44	58795.0
45	66157.0
46	75077.5
47	83998.0
48	90983.0
49	97968.0
50	97134.0
51	96300.0
52	88702.5
53	81105.0
54	75616.0
55	70127.0
56	67238.5
57	64350.0
58	63859.0
59	63368.0
60	60867.0
61	58366.0
62	56638.0
63	54910.0
64	47961.0
65	41012.0
66	36279.5
67	31547.0
68	25906.5
69	20266.0
70	17647.5
71	15029.0
72	13546.0
73	12063.0
74	10397.0
75	6694.5
76	4658.0
77	3945.0
78	3232.0
79	2485.5
80	1739.0
81	1215.5
82	692.0
83	561.0
84	430.0
85	349.0
86	268.0
87	146.5
88	25.0
89	17.0
90	9.0
91	8.0
92	7.0
93	4.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1142723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.181003768256794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20520390906557	18.42718322885892
2	9.404166570334594	4.5480437454915075
3	3.432513522036565	2.4900486723287574
4	1.6308639378426284	1.5774370810594684
5	0.9986263290363336	1.2073893512754015
6	0.6887759511058537	0.9993176321505185
7	0.5064095091226646	0.8571843173863357
8	0.3974356200817453	0.7688313781468927
9	0.29698588248891955	0.6463275069225269
>10	4.226318400685943	26.08403602304285
>50	1.9896414830863578	33.69828526530348
>100	0.2157574486115616	6.999388583380406
>500	0.005111005550866061	0.8587686931077115
>1k	0.002190430950371169	0.8377585215452491
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2228	0.1949728849423701	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1636	0.14316680420364342	No Hit
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1630	0.14264174257453469	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	1615	0.1413290885017629	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	1233	0.10790016478184127	Illumina Single End Adapter 1 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1148	0.10046179170280112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6253081455435834E-4	0.0	0.0	0.12041413360893234	0.0
2	2.6253081455435834E-4	0.0	0.0	0.5259367318238979	0.0
3	2.6253081455435834E-4	0.0	0.0	0.7078705863100682	0.0
4	2.6253081455435834E-4	0.0	0.0	0.935659823071733	0.0
5	2.6253081455435834E-4	0.0	0.0	1.638979875262859	0.0
6	2.6253081455435834E-4	0.0	0.0	2.2973196478936715	0.0
7	2.6253081455435834E-4	0.0	0.0	2.888451531998568	0.0
8	2.6253081455435834E-4	0.0	0.0	3.9628151354265206	0.0
9	2.6253081455435834E-4	0.0	0.0	4.516667643864698	0.0
10	2.6253081455435834E-4	0.0	0.0	5.496257623238527	0.0
11	2.6253081455435834E-4	0.0	0.0	6.4924745542007996	0.0
12	2.6253081455435834E-4	0.0	0.0	7.364776940693414	0.0
13	2.6253081455435834E-4	0.0	0.0	7.707292143415334	0.0
14	2.6253081455435834E-4	0.0	0.0	7.859384995313825	0.0
15	2.6253081455435834E-4	0.0	0.0	8.078598225466715	0.0
16	2.6253081455435834E-4	0.0	0.0	8.551503732750632	0.0
17	2.6253081455435834E-4	0.0	0.0	9.098880481096469	0.0
18	2.6253081455435834E-4	0.0	0.0	9.76850907875312	0.0
19	2.6253081455435834E-4	0.0	0.0	10.113912120435137	0.0
20	2.6253081455435834E-4	0.0	0.0	10.4692038227987	0.0
21	3.5004108607247775E-4	0.0	0.0	10.851886240147437	0.0
22	4.375513575905972E-4	0.0	0.0	11.258108920534548	0.0
23	4.375513575905972E-4	0.0	0.0	11.664594131736212	0.0
24	4.375513575905972E-4	0.0	0.0	11.99652059160444	0.0
25	4.375513575905972E-4	0.0	0.0	12.277691093992157	0.0
26	4.375513575905972E-4	0.0	0.0	12.556323798505849	0.0
27	4.375513575905972E-4	0.0	0.0	12.85088337243584	0.0
28	4.375513575905972E-4	0.0	0.0	13.143867761478504	0.0
29	5.250616291087167E-4	0.0	0.0	13.435539496448396	0.0
30	5.250616291087167E-4	0.0	0.0	13.788730952295525	0.0
31	5.250616291087167E-4	0.0	0.0	14.104905563290492	0.0
32	5.250616291087167E-4	0.0	0.0	14.409441308173546	0.0
33	5.250616291087167E-4	0.0	0.0	14.711876806540168	0.0
34	5.250616291087167E-4	0.0	0.0	15.017725205495996	0.0
35	5.250616291087167E-4	0.0	0.0	15.357527589800853	0.0
36	5.250616291087167E-4	0.0	0.0	15.670289300206612	0.0
37	5.250616291087167E-4	0.0	0.0	15.99468987672428	0.0
38	5.250616291087167E-4	0.0	0.0	16.30045076540859	0.0
39	5.250616291087167E-4	0.0	0.0	16.63824041346853	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCTA	25	3.8907907E-5	45.000004	17
TCAATCG	25	3.8907907E-5	45.000004	19
AGCGTAA	20	7.033269E-4	45.0	44
CACGGAT	20	7.033269E-4	45.0	45
ATTACGA	20	7.033269E-4	45.0	19
ACCGAAT	20	7.033269E-4	45.0	23
GTATCGT	20	7.033269E-4	45.0	29
ACGCATT	160	0.0	42.1875	17
ACCGCTA	160	0.0	42.1875	26
TACGCAT	160	0.0	42.1875	16
GCAAACG	65	0.0	41.538464	1
AAATGCG	65	0.0	41.538464	1
CGATCTA	50	1.0822987E-9	40.500004	10
GCTACGA	135	0.0	40.0	10
TCTAGCG	40	3.45819E-7	39.375	1
CTATGCG	35	6.2482577E-6	38.571426	1
TAACGAG	35	6.2482577E-6	38.571426	1
CGGATAC	35	6.2482577E-6	38.571426	23
TATTACG	35	6.2482577E-6	38.571426	1
TATAGCG	35	6.2482577E-6	38.571426	1
>>END_MODULE