##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552657_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	728661
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.701752941354073	31.0	31.0	34.0	30.0	34.0
2	31.846028537275906	33.0	31.0	34.0	30.0	34.0
3	31.895117208139315	33.0	31.0	34.0	30.0	34.0
4	35.540318474571855	37.0	35.0	37.0	33.0	37.0
5	35.34466507745028	37.0	35.0	37.0	33.0	37.0
6	35.35147620086707	37.0	35.0	37.0	33.0	37.0
7	35.71311350545727	37.0	35.0	37.0	35.0	37.0
8	35.50555059211348	37.0	35.0	37.0	35.0	37.0
9	37.17517473832139	39.0	37.0	39.0	34.0	39.0
10	36.755308710086034	39.0	37.0	39.0	32.0	39.0
11	36.706003203135616	39.0	35.0	39.0	32.0	39.0
12	36.59413225079975	39.0	35.0	39.0	32.0	39.0
13	36.40399307771378	39.0	35.0	39.0	32.0	39.0
14	37.526375090748644	39.0	36.0	41.0	32.0	41.0
15	37.665873430854674	40.0	36.0	41.0	33.0	41.0
16	37.72284779890786	40.0	36.0	41.0	33.0	41.0
17	37.69278443610952	40.0	36.0	41.0	33.0	41.0
18	37.65619814975688	39.0	36.0	41.0	33.0	41.0
19	37.62754147676354	39.0	36.0	41.0	33.0	41.0
20	37.53477405816971	39.0	36.0	41.0	32.0	41.0
21	37.43814338903825	39.0	35.0	41.0	32.0	41.0
22	37.454319635605586	39.0	35.0	41.0	32.0	41.0
23	37.354548960353306	39.0	35.0	41.0	32.0	41.0
24	37.371727044537856	39.0	35.0	41.0	32.0	41.0
25	37.203394994380105	39.0	35.0	41.0	32.0	41.0
26	37.18375897708262	39.0	35.0	41.0	32.0	41.0
27	36.94359379739001	39.0	35.0	41.0	31.0	41.0
28	36.95775264491993	39.0	35.0	41.0	31.0	41.0
29	36.92066406737838	39.0	35.0	41.0	31.0	41.0
30	36.76284856744083	39.0	35.0	41.0	31.0	41.0
31	36.74393442217986	39.0	35.0	41.0	31.0	41.0
32	36.62626104594592	39.0	35.0	40.0	31.0	41.0
33	36.49605097569377	39.0	35.0	40.0	30.0	41.0
34	36.424162950947014	39.0	35.0	40.0	30.0	41.0
35	36.2993298667007	38.0	35.0	40.0	30.0	41.0
36	36.20810363118103	38.0	35.0	40.0	30.0	41.0
37	36.14918185548561	38.0	35.0	40.0	30.0	41.0
38	36.046772092921124	38.0	35.0	40.0	30.0	41.0
39	36.00582438198284	38.0	35.0	40.0	30.0	41.0
40	35.89791549156604	38.0	35.0	40.0	29.0	41.0
41	35.810781694093684	38.0	35.0	40.0	29.0	41.0
42	35.75706123972602	38.0	35.0	40.0	29.0	41.0
43	35.67107475218243	38.0	34.0	40.0	29.0	41.0
44	35.58827355930947	38.0	34.0	40.0	29.0	41.0
45	35.529649590138625	37.0	34.0	40.0	28.0	41.0
46	35.42026539090194	37.0	34.0	40.0	28.0	41.0
47	35.33552227990794	37.0	34.0	40.0	28.0	41.0
48	35.24160343424445	37.0	34.0	40.0	28.0	41.0
49	35.16943818867759	37.0	34.0	40.0	28.0	41.0
50	35.072048593241576	36.0	34.0	40.0	28.0	41.0
51	34.33125417718253	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	26.0
10	42.0
11	52.0
12	32.0
13	25.0
14	51.0
15	69.0
16	100.0
17	205.0
18	336.0
19	521.0
20	896.0
21	1393.0
22	2099.0
23	2983.0
24	4170.0
25	5332.0
26	6737.0
27	8225.0
28	9805.0
29	11865.0
30	14864.0
31	19040.0
32	24672.0
33	33600.0
34	53615.0
35	68291.0
36	68378.0
37	94368.0
38	136512.0
39	160272.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51817923561162	19.65207414696272	24.047396525956515	12.782350091469146
2	30.44461004500035	25.634142625994805	26.093203835528456	17.82804349347639
3	31.65408880123953	24.974027702868685	26.54650104781236	16.825382448079424
4	28.895192689055676	26.087714314338218	26.012370636002203	19.0047223606039
5	25.16986637133043	30.59282711713678	23.92758772597957	20.309718785553226
6	24.662634613352438	35.742684183728784	23.657777759479373	15.936903443439403
7	79.90313739859826	6.995022376660752	8.188169807358978	4.913670417382019
8	81.27071436511629	6.193963996975274	6.948910398662752	5.5864112392456855
9	74.87638284469733	8.572161814616125	9.764760293195327	6.78669504749122
10	41.071774117182066	26.244302906289757	16.68279213516299	16.00113084136519
11	30.7560031345166	26.670426988681978	22.534210009867415	20.039359866934007
12	26.972350654145067	23.157545141018936	29.427676244508767	20.44242796032723
13	24.295111169666004	25.422384346081373	29.729874386031362	20.552630098221258
14	20.473032040962806	28.561567038718966	28.731193243497323	22.23420767682091
15	19.892103461005874	25.693154978789863	32.81155434420121	21.603187216003054
16	21.900582026484194	24.168440468201265	29.9590618957238	23.971915609590745
17	22.550815811467885	24.259292043899702	26.566537800156727	26.623354344475693
18	23.947624478323938	23.59245245731554	28.959969039100486	23.49995402526003
19	25.005180735623288	25.35280466499511	25.82490348735557	23.817111112026033
20	25.438990147681846	25.111951922773418	25.404817878272613	24.04424005127213
21	24.841593004154195	26.56708675227575	26.098830594748453	22.492489648821607
22	23.329641630332898	24.066335374062835	25.160808661366534	27.44321433423773
23	21.556663523915784	26.884518315101264	25.653630426220147	25.9051877347628
24	21.828669298892077	25.6674914672255	28.161929896069637	24.341909337812783
25	22.645647290029245	26.413105682889576	26.484743934422177	24.456503092659002
26	22.194408648191683	26.56557713394843	25.81886501404631	25.42114920381357
27	23.46482108964251	26.560499326847463	26.02829024745389	23.946389336056136
28	21.20355007335373	26.162783516614724	28.146421998707215	24.487244411324333
29	23.411709972126957	25.436657101175992	25.403719974034566	25.74791295266249
30	23.610979591332594	27.159268850672674	25.14777104853972	24.081980509455015
31	25.132674865266562	27.10821630360346	22.571813230020542	25.187295601109433
32	25.168219514973355	27.13003715033466	23.16221123403064	24.539532100661347
33	23.860341091399157	27.03259814920793	22.926161822850407	26.18089893654251
34	23.113903447556545	25.628104152685545	25.97147370313493	25.286518696622984
35	23.104708499562896	25.043744621984708	25.83780386215263	26.013743016299763
36	25.133910007534364	27.302408115708126	24.69406212216655	22.869619754590957
37	23.881338509951817	26.407616161699337	25.904089830524757	23.80695549782409
38	23.111844877110208	27.361420468503184	25.89036602754916	23.636368626837445
39	24.34094867160449	25.01190539908133	26.193113121190787	24.454032808123394
40	24.29085679074357	24.007048545208264	29.127536673432502	22.574557990615663
41	22.87250175321583	24.058650044396504	27.165856276100957	25.90299192628671
42	23.22767377422423	24.87260879887904	26.93762943261681	24.96208799427992
43	23.512717162027336	23.603568737725773	27.633151767419967	25.250562332826927
44	22.93755257932015	23.686597745728125	27.400396068953874	25.97545360599785
45	23.11980468283605	24.466246992771673	26.726831818911673	25.6871165054806
46	23.356265808105555	25.437755005414044	27.254374805293548	23.95160438118686
47	23.233163295414467	24.787383982400595	28.150950853689167	23.828501868495774
48	22.356761237392973	24.858473281814177	27.30007506920228	25.484690411590577
49	22.589379697829308	24.115192112655954	28.7515044719012	24.54392371761354
50	22.253558239016495	23.537282769353652	29.033665861079434	25.175493130550418
51	21.935852200131475	23.95874075873417	27.191382549635563	26.91402449149879
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1965.0
1	2114.5
2	2264.0
3	2642.0
4	3020.0
5	2233.0
6	1446.0
7	1425.5
8	1405.0
9	1553.5
10	1702.0
11	1798.5
12	1895.0
13	1897.0
14	1899.0
15	2079.5
16	2260.0
17	2099.0
18	1938.0
19	1944.0
20	1950.0
21	2021.5
22	2093.0
23	2340.5
24	2588.0
25	3363.5
26	5226.5
27	6314.0
28	6690.5
29	7067.0
30	7714.0
31	8361.0
32	9211.0
33	10061.0
34	12161.5
35	14262.0
36	15722.0
37	17182.0
38	18533.0
39	19884.0
40	22557.0
41	25230.0
42	30398.0
43	35566.0
44	40291.5
45	45017.0
46	51374.0
47	57731.0
48	63010.0
49	68289.0
50	65700.5
51	63112.0
52	57453.5
53	51795.0
54	47682.0
55	43569.0
56	41661.5
57	39754.0
58	37799.0
59	35844.0
60	34808.0
61	33772.0
62	31370.0
63	28968.0
64	26473.0
65	23978.0
66	20548.0
67	17118.0
68	15949.5
69	14781.0
70	12418.0
71	10055.0
72	8837.0
73	7619.0
74	6727.0
75	4543.0
76	3251.0
77	2636.0
78	2021.0
79	1423.5
80	826.0
81	684.0
82	542.0
83	374.0
84	206.0
85	134.0
86	62.0
87	38.5
88	15.0
89	10.0
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	728661.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.852009767442972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89278695043191	19.619810695210347
2	9.672273901448799	5.000954387472762
3	3.5147994722741984	2.72593890863508
4	1.7198401905403837	1.7784530161716396
5	0.9733401122535904	1.2581399044511932
6	0.6757200073677216	1.0481232138316188
7	0.5278018760848556	0.9551317479074293
8	0.3872811246843659	0.8009596334469222
9	0.3241502069904543	0.7541940885512299
>10	4.499848287398507	30.22046544537077
>50	1.6828712965771604	28.980807480695823
>100	0.11963031531294288	4.647901984552802
>500	0.007510423382875338	1.344554805163198
>1k	0.0021458352522500963	0.8645646885391826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1975	0.2710451087680005	TruSeq Adapter, Index 27 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1535	0.21066037567538265	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1425	0.1955641924022282	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1299	0.17827220065297855	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	888	0.12186737042328326	No Hit
CTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGCT	833	0.11431927878670603	TruSeq Adapter, Index 20 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	807	0.11075109001305135	No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	735	0.10086995187062298	TruSeq Adapter, Index 27 (95% over 21bp)
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	735	0.10086995187062298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.372380297559496E-4	0.0	0.0	0.1236514648101106	0.0
2	1.372380297559496E-4	0.0	0.0	0.6960712869221765	0.0
3	1.372380297559496E-4	0.0	0.0	0.966841919630665	0.0
4	1.372380297559496E-4	0.0	0.0	1.301702712235182	0.0
5	1.372380297559496E-4	0.0	0.0	2.3577493512072145	0.0
6	1.372380297559496E-4	0.0	0.0	3.1858436227546143	0.0
7	1.372380297559496E-4	0.0	0.0	3.919243653770409	0.0
8	1.372380297559496E-4	0.0	0.0	5.193773236113913	0.0
9	1.372380297559496E-4	0.0	0.0	5.8256171251103055	0.0
10	1.372380297559496E-4	0.0	0.0	7.040036450420703	0.0
11	1.372380297559496E-4	0.0	0.0	8.209029987881882	0.0
12	1.372380297559496E-4	0.0	0.0	9.273997098788051	0.0
13	1.372380297559496E-4	0.0	0.0	9.672536337199329	0.0
14	1.372380297559496E-4	0.0	0.0	9.832006927775742	0.0
15	1.372380297559496E-4	0.0	0.0	10.126245263572498	0.0
16	1.372380297559496E-4	0.0	0.0	10.725700977546486	0.0
17	2.744760595118992E-4	0.0	0.0	11.432476830789627	0.0
18	2.744760595118992E-4	0.0	0.0	12.322739929816471	0.0
19	2.744760595118992E-4	0.0	0.0	12.731023068340422	0.0
20	2.744760595118992E-4	0.0	0.0	13.154402390137527	0.0
21	2.744760595118992E-4	0.0	0.0	13.580663710559506	0.0
22	2.744760595118992E-4	0.0	0.0	13.990868181500039	0.0
23	2.744760595118992E-4	0.0	0.0	14.436891778206876	0.0
24	2.744760595118992E-4	0.0	0.0	14.810316457172814	0.0
25	2.744760595118992E-4	0.0	0.0	15.129532114385153	0.0
26	2.744760595118992E-4	0.0	0.0	15.44641472509164	0.0
27	2.744760595118992E-4	0.0	0.0	15.749024580703509	0.0
28	2.744760595118992E-4	0.0	0.0	16.06041767021976	0.0
29	2.744760595118992E-4	0.0	0.0	16.373869330182348	0.0
30	2.744760595118992E-4	0.0	0.0	16.788739894134583	0.0
31	2.744760595118992E-4	0.0	0.0	17.128129541721048	0.0
32	2.744760595118992E-4	0.0	0.0	17.462578620236297	0.0
33	2.744760595118992E-4	0.0	0.0	17.79798836495984	0.0
34	2.744760595118992E-4	0.0	0.0	18.13573115618923	0.0
35	2.744760595118992E-4	0.0	0.0	18.524114780398566	0.0
36	2.744760595118992E-4	0.0	0.0	18.86624918858015	0.0
37	2.744760595118992E-4	0.0	0.0	19.18752341623883	0.0
38	2.744760595118992E-4	0.0	0.0	19.527050301855045	0.0
39	2.744760595118992E-4	0.0	0.0	19.847912815424458	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAC	40	6.8084773E-9	45.000004	29
CTGCGTA	20	7.031584E-4	45.000004	40
CCGTATC	40	6.8084773E-9	45.000004	41
CTAACGA	20	7.031584E-4	45.000004	36
CGTAGAC	20	7.031584E-4	45.000004	43
CGCTAAC	20	7.031584E-4	45.000004	36
TTACGAG	20	7.031584E-4	45.000004	1
CCGAACC	20	7.031584E-4	45.000004	25
CGCGTGA	20	7.031584E-4	45.000004	41
CCAAACG	20	7.031584E-4	45.000004	1
AATCGGC	20	7.031584E-4	45.000004	19
AATCGCG	20	7.031584E-4	45.000004	1
TCGTTAG	35	1.2111013E-7	45.0	1
TCACCGA	25	3.889395E-5	45.0	21
GTATGCG	35	1.2111013E-7	45.0	1
CGGTCTA	35	1.2111013E-7	45.0	31
ATGTTCG	25	3.889395E-5	45.0	1
TGCGTAT	30	2.1644046E-6	44.999996	12
ACGCATT	185	0.0	43.783783	17
TACGAGG	105	0.0	40.714287	2
>>END_MODULE