##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552655_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	469093
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.676759192740032	31.0	31.0	34.0	30.0	34.0
2	31.832133500180134	33.0	31.0	34.0	30.0	34.0
3	31.879844721622366	33.0	31.0	34.0	30.0	34.0
4	35.52225891241182	37.0	35.0	37.0	33.0	37.0
5	35.32079566312011	37.0	35.0	37.0	33.0	37.0
6	35.31712048570326	37.0	35.0	37.0	32.0	37.0
7	35.67132956577907	37.0	35.0	37.0	35.0	37.0
8	35.44308484671483	37.0	35.0	37.0	33.0	37.0
9	37.11146403804789	39.0	37.0	39.0	33.0	39.0
10	36.740799798760584	39.0	37.0	39.0	32.0	39.0
11	36.70190559228128	39.0	35.0	39.0	32.0	39.0
12	36.576416616747636	39.0	35.0	39.0	32.0	39.0
13	36.383077556049656	39.0	35.0	39.0	32.0	39.0
14	37.48056355562756	39.0	36.0	41.0	32.0	41.0
15	37.601795379594236	39.0	36.0	41.0	33.0	41.0
16	37.654458710746056	39.0	36.0	41.0	33.0	41.0
17	37.63442430392268	39.0	36.0	41.0	33.0	41.0
18	37.602460492908655	39.0	36.0	41.0	33.0	41.0
19	37.52906992856427	39.0	36.0	41.0	32.0	41.0
20	37.450865819784134	39.0	35.0	41.0	32.0	41.0
21	37.35998618610809	39.0	35.0	41.0	32.0	41.0
22	37.39467440358308	39.0	35.0	41.0	32.0	41.0
23	37.295726007422836	39.0	35.0	41.0	32.0	41.0
24	37.27622454396037	39.0	35.0	41.0	32.0	41.0
25	37.09829820525994	39.0	35.0	41.0	32.0	41.0
26	37.07210510495786	39.0	35.0	41.0	32.0	41.0
27	36.85283088854449	39.0	35.0	41.0	31.0	41.0
28	36.860577753238694	39.0	35.0	41.0	31.0	41.0
29	36.82668468725818	39.0	35.0	41.0	31.0	41.0
30	36.68677639615172	39.0	35.0	40.0	31.0	41.0
31	36.66715555337641	39.0	35.0	40.0	31.0	41.0
32	36.50177896493872	38.0	35.0	40.0	30.0	41.0
33	36.397475553888036	38.0	35.0	40.0	30.0	41.0
34	36.25688296350617	38.0	35.0	40.0	30.0	41.0
35	36.10553131255423	38.0	35.0	40.0	30.0	41.0
36	36.02331520615315	38.0	35.0	40.0	30.0	41.0
37	35.98094407718725	38.0	35.0	40.0	30.0	41.0
38	35.83513290541534	38.0	35.0	40.0	29.0	41.0
39	35.83368329947366	38.0	35.0	40.0	29.0	41.0
40	35.67305630226842	38.0	35.0	40.0	29.0	41.0
41	35.57309744549588	38.0	34.0	40.0	29.0	41.0
42	35.509602573476904	38.0	34.0	40.0	29.0	41.0
43	35.504716548744064	38.0	34.0	40.0	29.0	41.0
44	35.44971253035112	37.0	34.0	40.0	29.0	41.0
45	35.424694037216504	37.0	34.0	40.0	29.0	41.0
46	35.32563692061063	37.0	34.0	40.0	28.0	41.0
47	35.21043588371602	37.0	34.0	40.0	28.0	41.0
48	35.11564231399744	37.0	34.0	40.0	28.0	41.0
49	35.0924251694227	37.0	34.0	40.0	28.0	41.0
50	35.002197858420395	37.0	34.0	40.0	27.0	41.0
51	34.2860584148559	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	19.0
10	26.0
11	24.0
12	22.0
13	24.0
14	33.0
15	49.0
16	71.0
17	120.0
18	214.0
19	391.0
20	689.0
21	1070.0
22	1622.0
23	2208.0
24	2925.0
25	3761.0
26	4301.0
27	5494.0
28	6264.0
29	7596.0
30	9760.0
31	12231.0
32	15706.0
33	21850.0
34	35651.0
35	46164.0
36	44790.0
37	61290.0
38	87313.0
39	97349.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.35297904679882	20.320277642173302	23.905707397040672	15.421035913987206
2	28.89704173799225	27.270498600490733	25.589808417520622	18.242651243996395
3	30.33897329527407	27.117650444581354	25.82131901350052	16.722057246644056
4	28.921557132594177	27.05391041861635	25.427793635803564	18.59673881298591
5	24.63690568821108	30.925850524309677	24.344639549087283	20.09260423839196
6	24.682738817249458	36.06449041021717	24.085629075684352	15.167141696849026
7	78.43028994250606	7.2354522450772025	9.150424329503958	5.183833482912769
8	78.91825288375652	6.9495814262843405	8.014828616073997	6.117337073885136
9	73.68325683819627	9.121858565359107	9.943444050540084	7.2514405459045435
10	41.16369248741721	25.71323809990769	16.95697868013379	16.166090732541306
11	31.6822037421151	26.636935533039292	21.773294421362074	19.90756630348353
12	26.901914972084427	22.1231184434643	29.493725124868632	21.48124145958264
13	24.41115088052902	25.00655520333921	30.099148782863956	20.483145133267815
14	20.72659366053213	29.78663079602552	27.549974098952664	21.936801444489685
15	18.482475756406515	25.753528617992593	33.552408584225304	22.21158704137559
16	21.504264612774012	24.130609495345272	31.10406678419844	23.26105910768227
17	21.574613136414314	23.758615029429134	26.731799451281518	27.93497238287504
18	23.414546795624748	23.84559138592987	28.169893816364773	24.56996800208061
19	24.113981662484836	26.631606099430176	26.120406827643983	23.134005410441
20	25.627540807473142	24.80510261291471	25.69319942953743	23.87415715007472
21	23.944718851059385	27.385401189103227	25.97075633190007	22.69912362793732
22	21.358238131884296	24.634347560078705	26.271549564798452	27.735864743238547
23	21.068530120892873	26.983775072320416	26.29116188047999	25.656532926306724
24	21.89565821702733	25.17667072414212	28.33595896762476	24.591712091205796
25	22.325636920610627	26.422692301952917	26.240425672521226	25.01124510491523
26	20.85471324449523	27.697705998597293	26.128933921418568	25.318646835488913
27	22.26168371730126	26.734570756758252	25.951570370907262	25.052175155033225
28	20.04250756246629	26.951372115977	28.2106106891384	24.795509632418305
29	21.76689910103114	26.40904895191359	26.116569635445426	25.70748231160985
30	22.930847401261584	26.90532580959426	25.494304967245302	24.669521821898858
31	24.373844845265225	27.115945025826438	22.646895178568002	25.86331495034034
32	23.990551980097763	28.50991168062623	23.722588058231523	23.776948281044483
33	24.705122438407738	25.42587503970428	23.36402376501035	26.504978756877634
34	23.62601872123438	26.83092691641103	24.74988968072429	24.793164681630294
35	23.952606412800872	24.81128475590128	25.763547953177728	25.472560878120117
36	26.210794021654554	26.94604268236789	23.770979315402275	23.072183980575282
37	24.857331062284025	26.528428264757736	26.04089167819601	22.57334899476223
38	23.81468067099701	27.639082228897045	25.913411626266008	22.632825473839944
39	26.865674823542452	23.201582628604562	26.46404870675964	23.468693841093344
40	25.570622456527808	23.06621501493307	28.785763164233956	22.57739936430516
41	22.768619442200162	24.379813810907432	26.52352518583735	26.32804156105506
42	23.26191181705973	24.56527810050459	27.10677840001876	25.066031682416916
43	23.906133751729403	23.91785850566945	27.502861905848093	24.67314583675305
44	23.809777592076625	24.041288188056527	27.21549884564468	24.933435374222167
45	23.625805543890017	23.671638672928395	26.426742671495845	26.275813111685743
46	25.260662597821753	24.477875389315127	26.52437789521481	23.737084117648312
47	23.98905973868721	24.863513205270596	27.748868561244784	23.398558494797406
48	22.220114135150173	23.768421187269904	28.230009827475577	25.781454850104353
49	23.13997437608321	23.31627203987269	29.302505046973625	24.241248537070476
50	22.33949344799432	22.656274981720042	29.149017359031156	25.855214211254484
51	21.877964497445067	23.28749309838348	26.438254248091532	28.396288156079923
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1649.0
1	1788.5
2	1928.0
3	2041.0
4	2154.0
5	1615.5
6	1077.0
7	1093.0
8	1109.0
9	1226.0
10	1343.0
11	1312.5
12	1282.0
13	1254.0
14	1226.0
15	1273.0
16	1320.0
17	1283.0
18	1246.0
19	1275.0
20	1304.0
21	1323.5
22	1343.0
23	1344.5
24	1346.0
25	1647.0
26	2450.0
27	2952.0
28	3577.0
29	4202.0
30	4460.0
31	4718.0
32	5577.5
33	6437.0
34	7797.5
35	9158.0
36	9655.0
37	10152.0
38	11160.5
39	12169.0
40	14171.5
41	16174.0
42	18988.0
43	21802.0
44	25911.5
45	30021.0
46	35338.5
47	40656.0
48	43708.5
49	46761.0
50	45605.5
51	44450.0
52	38017.0
53	31584.0
54	29360.5
55	27137.0
56	26484.0
57	25831.0
58	23544.5
59	21258.0
60	21047.5
61	20837.0
62	19705.5
63	18574.0
64	17042.5
65	15511.0
66	13664.5
67	11818.0
68	10300.5
69	8783.0
70	7367.5
71	5952.0
72	5126.0
73	4300.0
74	3920.5
75	2658.5
76	1776.0
77	1352.5
78	929.0
79	796.0
80	663.0
81	571.5
82	480.0
83	300.5
84	121.0
85	76.0
86	31.0
87	30.5
88	30.0
89	18.5
90	7.0
91	4.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	469093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.21621413813769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.06666088334003	22.515960672789024
2	9.098388533103147	5.316409353902361
3	3.3479909222497355	2.934468591509681
4	1.637920800106979	1.914153793489413
5	0.9612950190035058	1.4042700562565784
6	0.663194077612244	1.162561211199842
7	0.487249202099991	0.9964903919033047
8	0.37682388955381463	0.8807493959656156
9	0.33151169012314824	0.871696487514046
>10	4.9454027264913725	37.763213473473925
>50	1.0090856052754549	18.371640060022656
>100	0.06425436560364692	3.191571144194136
>500	0.00657146920946389	1.4292845843259614
>1k	0.0036508162274799384	1.247530783453466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	1348	0.2873630602034138	TruSeq Adapter, Index 14 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1270	0.2707352273429789	No Hit
CCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	1155	0.24621983274105563	TruSeq Adapter, Index 14 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1063	0.22660751705951698	No Hit
CTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGCT	1012	0.21573547249692493	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCG	986	0.21019286154344663	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGC	931	0.19846810760339634	No Hit
GAATCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC	921	0.19633633415975082	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	908	0.19356502868301168	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	775	0.16521244188252648	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCC	581	0.12385603707580373	No Hit
TCTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTCTGC	572	0.12193744097652279	TruSeq Adapter, Index 14 (95% over 21bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	517	0.11021268703647251	No Hit
AAAACTGTCTCTTATACACATCTGACGCACATGGTGTCGTATGCCGTCTTC	509	0.10850726828155612	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2635468872910065E-4	0.0	0.0	0.22831293581443338	0.0
2	4.2635468872910065E-4	0.0	0.0	1.0328442334462462	0.0
3	4.2635468872910065E-4	0.0	0.0	1.3724357430189749	0.0
4	4.2635468872910065E-4	0.0	0.0	1.7962323036157009	0.0
5	4.2635468872910065E-4	0.0	0.0	3.184229992773288	0.0
6	4.2635468872910065E-4	0.0	0.0	4.213450211365337	0.0
7	4.2635468872910065E-4	0.0	0.0	5.151856881258087	0.0
8	4.2635468872910065E-4	0.0	0.0	6.563730432984504	0.0
9	4.2635468872910065E-4	0.0	0.0	7.272758280340999	0.0
10	4.2635468872910065E-4	0.0	0.0	8.696356586007466	0.0
11	4.2635468872910065E-4	0.0	0.0	10.110575088521893	0.0
12	4.2635468872910065E-4	0.0	0.0	11.511576595685717	0.0
13	4.2635468872910065E-4	0.0	0.0	12.007000743988932	0.0
14	4.2635468872910065E-4	0.0	0.0	12.218899024287294	0.0
15	4.2635468872910065E-4	0.0	0.0	12.573412947965542	0.0
16	4.2635468872910065E-4	0.0	0.0	13.252382789766635	0.0
17	4.2635468872910065E-4	0.0	0.0	14.081642659344736	0.0
18	4.2635468872910065E-4	0.0	0.0	15.187606721908022	0.0
19	4.2635468872910065E-4	0.0	0.0	15.714581117177191	0.0
20	4.2635468872910065E-4	0.0	0.0	16.20744713734803	0.0
21	4.2635468872910065E-4	0.0	0.0	16.718859586478587	0.0
22	4.2635468872910065E-4	0.0	0.0	17.238799129383725	0.0
23	4.2635468872910065E-4	0.0	0.0	17.760657268388144	0.0
24	4.2635468872910065E-4	0.0	0.0	18.165907400025155	0.0
25	4.2635468872910065E-4	0.0	0.0	18.526603466689973	0.0
26	4.2635468872910065E-4	0.0	0.0	18.847861724647352	0.0
27	4.2635468872910065E-4	0.0	0.0	19.198325278782672	0.0
28	4.2635468872910065E-4	0.0	0.0	19.554331443871472	0.0
29	4.2635468872910065E-4	0.0	0.0	19.914174801158833	0.0
30	4.2635468872910065E-4	0.0	0.0	20.315800917941644	0.0
31	4.2635468872910065E-4	0.0	0.0	20.6660512947326	0.0
32	4.2635468872910065E-4	0.0	0.0	21.034421745794543	0.0
33	4.2635468872910065E-4	0.0	0.0	21.386164363996052	0.0
34	4.2635468872910065E-4	0.0	0.0	21.733003903277176	0.0
35	4.2635468872910065E-4	0.0	0.0	22.115657236411543	0.0
36	4.2635468872910065E-4	0.0	0.0	22.456954164739187	0.0
37	4.2635468872910065E-4	0.0	0.0	22.78268914692822	0.0
38	4.2635468872910065E-4	0.0	0.0	23.08433508920406	0.0
39	4.2635468872910065E-4	0.0	0.0	23.40751194326072	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.0290104E-4	45.000004	1
TTAGGCG	20	7.0290104E-4	45.000004	1
TCGTTGC	40	6.7993824E-9	45.000004	16
TCGTTGA	20	7.0290104E-4	45.000004	24
GCCCATA	20	7.0290104E-4	45.000004	18
ATTAGTA	20	7.0290104E-4	45.000004	12
CTCAACT	20	7.0290104E-4	45.000004	22
TTTCGTA	20	7.0290104E-4	45.000004	24
GCGCAAT	20	7.0290104E-4	45.000004	34
ATCGTTG	20	7.0290104E-4	45.000004	23
TGTTGCG	20	7.0290104E-4	45.000004	1
CGTAATA	20	7.0290104E-4	45.000004	32
CGTCCGA	20	7.0290104E-4	45.000004	16
TGTCGTT	20	7.0290104E-4	45.000004	26
TTCGACA	20	7.0290104E-4	45.000004	15
ATATTAC	20	7.0290104E-4	45.000004	41
GACTATA	20	7.0290104E-4	45.000004	16
TAGTAGA	20	7.0290104E-4	45.000004	35
AGGTATA	25	3.8872626E-5	45.0	39
ACTATCC	25	3.8872626E-5	45.0	40
>>END_MODULE