SRR3552654_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1392590
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	2721	0.19539132120724692	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2371	0.1702582956936356	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	2316	0.1663088202557824	TruSeq Adapter, Index 21 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1930	0.13859068354648532	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1750	0.12566512756805664	TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1626	0.11676085567180579	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.0338015E-4	45.0	13
GTCGATC	20	7.0338015E-4	45.0	24
ACGATTG	20	7.0338015E-4	45.0	1
TTAGTCG	20	7.0338015E-4	45.0	37
CGCCCTA	20	7.0338015E-4	45.0	16
TCGCGAT	20	7.0338015E-4	45.0	42
TCGTTAG	30	2.1658343E-6	44.999996	1
GCTCGAT	30	2.1658343E-6	44.999996	45
TCGAACG	60	3.6379788E-12	41.249996	1
GACCTAT	255	0.0	40.588234	33
TATAGCG	50	1.0822987E-9	40.500004	1
TCGACAG	90	0.0	40.000004	1
GCTCGTA	210	0.0	39.642857	32
CGAATAT	285	0.0	39.473686	14
CGTAAGG	240	0.0	39.374996	2
TACGAAT	285	0.0	38.68421	12
AATCCGT	30	1.1400459E-4	37.499996	42
CTATCGC	30	1.1400459E-4	37.499996	16
CCTACGA	30	1.1400459E-4	37.499996	32
ACTCGAA	30	1.1400459E-4	37.499996	43