##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552654_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1392590
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85750149002937	31.0	31.0	34.0	30.0	34.0
2	31.999317099792474	33.0	31.0	34.0	30.0	34.0
3	32.02472802475962	33.0	31.0	34.0	30.0	34.0
4	35.66681148076606	37.0	35.0	37.0	33.0	37.0
5	35.484545343568456	37.0	35.0	37.0	33.0	37.0
6	35.49274589075033	37.0	35.0	37.0	33.0	37.0
7	35.85999971276542	37.0	35.0	37.0	35.0	37.0
8	35.68813290343891	37.0	35.0	37.0	35.0	37.0
9	37.37201904365247	39.0	37.0	39.0	34.0	39.0
10	36.930737690203145	39.0	37.0	39.0	33.0	39.0
11	36.889164075571415	39.0	37.0	39.0	33.0	39.0
12	36.84687309258288	39.0	37.0	39.0	33.0	39.0
13	36.71945080748821	39.0	37.0	39.0	33.0	39.0
14	37.86702331626681	40.0	37.0	41.0	33.0	41.0
15	37.98845676042482	40.0	37.0	41.0	33.0	41.0
16	38.01379587674764	40.0	37.0	41.0	33.0	41.0
17	37.98797636059429	40.0	37.0	41.0	33.0	41.0
18	37.94771468989437	40.0	37.0	41.0	33.0	41.0
19	37.9198177496607	40.0	37.0	41.0	33.0	41.0
20	37.871423749991024	40.0	36.0	41.0	33.0	41.0
21	37.78597936219562	40.0	36.0	41.0	33.0	41.0
22	37.81035696077094	40.0	36.0	41.0	33.0	41.0
23	37.73067090816392	40.0	36.0	41.0	33.0	41.0
24	37.73838818316949	40.0	36.0	41.0	33.0	41.0
25	37.59039846616736	39.0	36.0	41.0	33.0	41.0
26	37.61995993077647	40.0	36.0	41.0	33.0	41.0
27	37.41221680465895	39.0	36.0	41.0	32.0	41.0
28	37.42137815150188	39.0	36.0	41.0	32.0	41.0
29	37.387610854594676	39.0	36.0	41.0	32.0	41.0
30	37.26213817419341	39.0	35.0	41.0	32.0	41.0
31	37.25158876625568	39.0	35.0	41.0	32.0	41.0
32	37.11331475883067	39.0	35.0	41.0	31.0	41.0
33	37.02766068979384	39.0	35.0	41.0	31.0	41.0
34	36.962490754637045	39.0	35.0	41.0	31.0	41.0
35	36.82017104819078	39.0	35.0	41.0	31.0	41.0
36	36.79345895058847	39.0	35.0	41.0	31.0	41.0
37	36.749313150317036	39.0	35.0	41.0	31.0	41.0
38	36.61647003066229	39.0	35.0	41.0	30.0	41.0
39	36.60549336129083	39.0	35.0	41.0	31.0	41.0
40	36.5290099742207	39.0	35.0	41.0	30.0	41.0
41	36.453127625503555	39.0	35.0	41.0	30.0	41.0
42	36.41623378022246	39.0	35.0	40.0	30.0	41.0
43	36.38635779375121	39.0	35.0	40.0	30.0	41.0
44	36.33137894139696	38.0	35.0	40.0	30.0	41.0
45	36.2733295514114	38.0	35.0	40.0	30.0	41.0
46	36.160402559260085	38.0	35.0	40.0	30.0	41.0
47	36.078885386222794	38.0	35.0	40.0	30.0	41.0
48	35.99062322722409	38.0	35.0	40.0	30.0	41.0
49	35.952628555425505	38.0	35.0	40.0	30.0	41.0
50	35.87846674182638	38.0	35.0	40.0	29.0	41.0
51	35.15984029757502	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	37.0
10	73.0
11	62.0
12	56.0
13	55.0
14	65.0
15	95.0
16	146.0
17	220.0
18	375.0
19	716.0
20	1202.0
21	1883.0
22	2792.0
23	4240.0
24	5843.0
25	7817.0
26	10133.0
27	12644.0
28	15363.0
29	18853.0
30	24135.0
31	31003.0
32	40988.0
33	56245.0
34	90502.0
35	113989.0
36	123511.0
37	178290.0
38	280193.0
39	370886.0
40	167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95421480837863	19.351855176326126	23.306285410637734	13.387644604657508
2	30.96331296361456	25.268815660029155	25.958537688766974	17.809333687589312
3	31.33549716714898	25.55224437917837	26.710948664000174	16.40130978967248
4	28.58407715120746	26.414019919717934	26.0441335928019	18.9577693362727
5	25.34342484148242	30.347697455819734	24.787195082544038	19.52168262015381
6	25.464494215813698	35.05087642450398	24.017334606739958	15.46729475294236
7	82.6314277712751	5.949992460092346	7.186465506717699	4.23211426191485
8	83.98229198830956	5.008222089775167	6.492219533387429	4.517266388527851
9	77.44677184239438	7.820176792882327	9.075176469743427	5.657874894979858
10	36.93183205394266	31.401130268061667	16.593254295952146	15.07378338204353
11	28.368435792300676	27.55584917312346	25.12282868611723	18.952886348458627
12	25.548653946962137	24.88973782663957	29.48197243984231	20.079635786555986
13	24.28108775734423	25.888596069194808	30.166165202967132	19.66415097049383
14	21.102047264449695	27.895145017557216	29.510408662994852	21.49239905499824
15	20.766341852232173	26.90404210858903	31.524856562232962	20.804759476945836
16	23.563360357319816	26.007367566907703	29.767196375099637	20.662075700672847
17	23.36035731981416	25.355847736950576	27.373455216538968	23.9103397266963
18	25.100352580443637	24.551375494582036	28.871814389016148	21.47645753595818
19	23.80413474174021	27.228760798224886	27.11803186867635	21.84907259135855
20	25.47375753093157	26.355639491882034	26.949281554513533	21.22132142267286
21	24.415298113586914	28.09391134504772	27.44210428051329	20.04868626085208
22	22.782728584867044	25.737079829669895	27.174186228538193	24.306005356924867
23	21.37635628576968	28.028924521933952	27.630817397798346	22.96390179449802
24	21.812521991397325	26.397001271013004	29.025413079226475	22.765063658363193
25	21.619859398674414	28.129815667210018	27.578756130663006	22.671568803452562
26	21.669838215124337	27.643814762421098	27.365125413797315	23.32122160865725
27	22.017033010433796	26.310615471890507	28.48907431476601	23.183277202909686
28	20.623155415448913	27.54945820377857	29.06533868547096	22.76204769530156
29	21.978974428941754	26.10969488507027	28.34323095814274	23.568099727845237
30	23.279500786304656	25.97246856576595	28.382510286588296	22.365520361341098
31	24.243603645006786	27.47334104079449	25.672380241133432	22.610675073065295
32	23.55488693728951	27.051178020810145	27.484327763376154	21.909607278524188
33	23.59014498165289	26.55095900444496	25.886154575287772	23.97274143861438
34	22.606941023560417	25.852189086522237	28.883806432618357	22.65706345729899
35	21.865301344975908	26.03458304310673	28.930625668718	23.169489943199363
36	24.33523147516498	27.490862350009692	26.311979836132675	21.86192633869265
37	23.621668976511394	27.361965833447034	27.493878313071328	21.52248687697025
38	22.5240020393655	28.006232990327373	28.474497159968116	20.99526781033901
39	24.213659440323426	26.22394243819071	27.48569212761832	22.07670599386754
40	24.920328309121853	25.054036004854265	28.901040507256265	21.12459517876762
41	21.841532683704465	26.589951098313215	28.14862953202307	23.419886685959256
42	22.123525229967182	26.440732735406687	28.547311125313264	22.888430909312863
43	22.43553378955759	25.896351402781864	28.59204791072749	23.076066896933053
44	23.09818395938503	25.72200001436173	27.698461140752123	23.481354885501116
45	23.39547174688889	24.90000646277799	27.70700636942675	23.997515420906367
46	23.022784882844196	26.065388951521985	27.512835795173025	23.398990370460794
47	22.11052786534443	26.86002340961805	29.217429394150468	21.812019330887054
48	21.467553264061927	26.31657558936945	29.161275034288625	23.054596112279995
49	22.995928449866796	25.33847004502402	28.893716025535156	22.77188547957403
50	21.90917642665824	25.283177388894075	29.362339238397517	23.445306946050167
51	21.474805937138715	24.875160671841677	27.788150137513558	25.861883253506058
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2134.0
1	2522.5
2	2911.0
3	4100.5
4	5290.0
5	3942.5
6	2595.0
7	2565.0
8	2535.0
9	2673.5
10	2812.0
11	3103.5
12	3395.0
13	3326.0
14	3257.0
15	3302.0
16	3347.0
17	3408.5
18	3470.0
19	3390.5
20	3311.0
21	3636.5
22	3962.0
23	5110.0
24	6258.0
25	7986.5
26	10465.5
27	11216.0
28	14873.5
29	18531.0
30	21033.5
31	23536.0
32	27318.0
33	31100.0
34	35668.0
35	40236.0
36	43396.0
37	46556.0
38	50246.5
39	53937.0
40	58325.5
41	62714.0
42	70977.5
43	79241.0
44	85940.5
45	92640.0
46	102539.0
47	112438.0
48	120851.0
49	129264.0
50	129504.5
51	129745.0
52	117380.0
53	105015.0
54	94114.5
55	83214.0
56	76094.5
57	68975.0
58	62577.0
59	56179.0
60	53143.0
61	50107.0
62	44534.0
63	38961.0
64	35492.5
65	32024.0
66	26800.5
67	21577.0
68	17850.0
69	14123.0
70	13275.5
71	12428.0
72	10542.0
73	8656.0
74	7453.5
75	4959.0
76	3667.0
77	3272.0
78	2877.0
79	2143.0
80	1409.0
81	1018.0
82	627.0
83	430.0
84	233.0
85	169.0
86	105.0
87	58.0
88	11.0
89	5.5
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1392590.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.086800683685006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.66129758754339	18.98099892047678
2	9.615357605451937	4.824371195006549
3	3.415109745348249	2.5702253248338534
4	1.6870435027988504	1.6929009639768215
5	1.039385485946794	1.303742825973115
6	0.7097823114644827	1.0683700425908826
7	0.49304378107635266	0.865822374493502
8	0.4158758270259523	0.8346395185410184
9	0.3370286138798123	0.7609472694991337
>10	4.68382687517924	30.323913909793674
>50	1.8102880883876042	30.576263152902595
>100	0.12735059944738342	4.755943608165457
>500	0.0025931117531032095	0.44691326980326784
>1k	0.002016864696858052	0.9949476239433902
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	2721	0.19539132120724692	TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2371	0.1702582956936356	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGC	2316	0.1663088202557824	TruSeq Adapter, Index 21 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1930	0.13859068354648532	No Hit
CTGTCTCTTATACACATCTGACGCTCGTGGTATCGTATGCCGTCTTCTGCT	1750	0.12566512756805664	TruSeq Adapter, Index 15 (95% over 22bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1626	0.11676085567180579	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13535929455187815	0.0
2	0.0	0.0	0.0	0.6403894900868167	0.0
3	0.0	0.0	0.0	0.8827436646823544	0.0
4	0.0	0.0	0.0	1.1526005500542156	0.0
5	0.0	0.0	0.0	1.9909664725439649	0.0
6	0.0	0.0	0.0	2.7619758866572357	0.0
7	0.0	0.0	0.0	3.3745754313904306	0.0
8	7.180864432460379E-5	0.0	0.0	4.380399112445156	0.0
9	7.180864432460379E-5	0.0	0.0	4.9229852289618625	0.0
10	7.180864432460379E-5	0.0	0.0	5.951500441623162	0.0
11	7.180864432460379E-5	0.0	0.0	7.116595695789859	0.0
12	7.180864432460379E-5	0.0	0.0	8.089171974522293	0.0
13	7.180864432460379E-5	0.0	0.0	8.490079635786556	0.0
14	7.180864432460379E-5	0.0	0.0	8.659260801815323	0.0
15	7.180864432460379E-5	0.0	0.0	8.929333113120157	0.0
16	7.180864432460379E-5	0.0	0.0	9.462153254008717	0.0
17	7.180864432460379E-5	0.0	0.0	10.08968899676143	0.0
18	7.180864432460379E-5	0.0	0.0	10.817182372413992	0.0
19	7.180864432460379E-5	0.0	0.0	11.216007582992841	1.4361728864920758E-4
20	7.180864432460379E-5	0.0	0.0	11.616771626968454	1.4361728864920758E-4
21	7.180864432460379E-5	0.0	0.0	12.07088949367725	1.4361728864920758E-4
22	7.180864432460379E-5	0.0	0.0	12.551361132853172	1.4361728864920758E-4
23	7.180864432460379E-5	0.0	0.0	13.02860138303449	1.4361728864920758E-4
24	7.180864432460379E-5	0.0	0.0	13.406458469470556	1.4361728864920758E-4
25	7.180864432460379E-5	0.0	0.0	13.746328783058905	1.4361728864920758E-4
26	7.180864432460379E-5	0.0	0.0	14.053669780768208	1.4361728864920758E-4
27	7.180864432460379E-5	0.0	0.0	14.38592837805815	1.4361728864920758E-4
28	7.180864432460379E-5	0.0	0.0	14.721849216208648	1.4361728864920758E-4
29	7.180864432460379E-5	0.0	0.0	15.074501468486776	1.4361728864920758E-4
30	7.180864432460379E-5	0.0	0.0	15.518566124990127	1.4361728864920758E-4
31	7.180864432460379E-5	0.0	0.0	15.875311469994758	1.4361728864920758E-4
32	7.180864432460379E-5	0.0	0.0	16.240243000452395	1.4361728864920758E-4
33	7.180864432460379E-5	0.0	0.0	16.606682512440848	1.4361728864920758E-4
34	7.180864432460379E-5	0.0	0.0	16.955098054703825	1.4361728864920758E-4
35	7.180864432460379E-5	0.0	0.0	17.338197172175587	1.4361728864920758E-4
36	7.180864432460379E-5	0.0	0.0	17.655734997378985	1.4361728864920758E-4
37	7.180864432460379E-5	0.0	0.0	18.004294156930612	1.4361728864920758E-4
38	7.180864432460379E-5	0.0	0.0	18.347180433580593	1.4361728864920758E-4
39	1.4361728864920758E-4	0.0	0.0	18.72288326068692	1.4361728864920758E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAC	20	7.0338015E-4	45.0	13
GTCGATC	20	7.0338015E-4	45.0	24
ACGATTG	20	7.0338015E-4	45.0	1
TTAGTCG	20	7.0338015E-4	45.0	37
CGCCCTA	20	7.0338015E-4	45.0	16
TCGCGAT	20	7.0338015E-4	45.0	42
TCGTTAG	30	2.1658343E-6	44.999996	1
GCTCGAT	30	2.1658343E-6	44.999996	45
TCGAACG	60	3.6379788E-12	41.249996	1
GACCTAT	255	0.0	40.588234	33
TATAGCG	50	1.0822987E-9	40.500004	1
TCGACAG	90	0.0	40.000004	1
GCTCGTA	210	0.0	39.642857	32
CGAATAT	285	0.0	39.473686	14
CGTAAGG	240	0.0	39.374996	2
TACGAAT	285	0.0	38.68421	12
AATCCGT	30	1.1400459E-4	37.499996	42
CTATCGC	30	1.1400459E-4	37.499996	16
CCTACGA	30	1.1400459E-4	37.499996	32
ACTCGAA	30	1.1400459E-4	37.499996	43
>>END_MODULE