SRR3552653_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716724
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	2786	0.38871308899939166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	2782	0.38815499411209897	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	2483	0.3464374012869668	RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2260	0.31532361132039666	RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1914	0.26704840356957493	RNA PCR Primer, Index 36 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1352	0.18863607190494527	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1329	0.18542702630301203	No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	1155	0.16114989870577795	RNA PCR Primer, Index 36 (96% over 26bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1111	0.15501085494555783	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	757	0.10561945742015057	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.211065E-7	45.000004	1
GTCGATC	20	7.0315064E-4	45.000004	8
AGTCAAC	20	7.0315064E-4	45.000004	37
CGAAATA	20	7.0315064E-4	45.000004	28
CGATTGT	40	6.8084773E-9	45.000004	10
TCGATGA	20	7.0315064E-4	45.000004	20
GCTTGAC	20	7.0315064E-4	45.000004	26
CGTTAGC	20	7.0315064E-4	45.000004	31
GCCGATA	30	2.1643536E-6	45.000004	9
CTAACGG	20	7.0315064E-4	45.000004	2
TCGGAAC	20	7.0315064E-4	45.000004	34
GTTAACG	20	7.0315064E-4	45.000004	1
TGTTACG	35	1.211065E-7	45.000004	26
TTACGAA	20	7.0315064E-4	45.000004	24
CGGTCGA	20	7.0315064E-4	45.000004	6
CGTCCAT	20	7.0315064E-4	45.000004	20
GACTAGT	20	7.0315064E-4	45.000004	2
TGCGTAT	30	2.1643536E-6	45.000004	15
ATTAGCG	45	3.8380676E-10	45.0	1
AACCGCT	25	3.889332E-5	44.999996	45