##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552653_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	716724
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63597005262835	31.0	31.0	34.0	30.0	34.0
2	31.76709863210943	31.0	31.0	34.0	30.0	34.0
3	31.82737008946261	33.0	31.0	34.0	30.0	34.0
4	35.48760889826488	37.0	35.0	37.0	33.0	37.0
5	35.293982062830324	37.0	35.0	37.0	33.0	37.0
6	35.287629547775715	37.0	35.0	37.0	32.0	37.0
7	35.647320307398665	37.0	35.0	37.0	35.0	37.0
8	35.388422879658	37.0	35.0	37.0	33.0	37.0
9	37.07112779814824	39.0	37.0	39.0	33.0	39.0
10	36.69244227903628	39.0	37.0	39.0	32.0	39.0
11	36.65058237201489	39.0	35.0	39.0	32.0	39.0
12	36.518839888157785	39.0	35.0	39.0	32.0	39.0
13	36.30230883854873	39.0	35.0	39.0	32.0	39.0
14	37.394242414095245	39.0	36.0	41.0	32.0	41.0
15	37.55683219761024	39.0	36.0	41.0	32.0	41.0
16	37.62044803857552	39.0	36.0	41.0	33.0	41.0
17	37.55391615182413	39.0	36.0	41.0	32.0	41.0
18	37.53503719702424	39.0	36.0	41.0	32.0	41.0
19	37.49149184344322	39.0	36.0	41.0	32.0	41.0
20	37.365054051489835	39.0	35.0	41.0	32.0	41.0
21	37.29845519335198	39.0	35.0	41.0	32.0	41.0
22	37.329022050328994	39.0	35.0	41.0	32.0	41.0
23	37.17463207594555	39.0	35.0	41.0	32.0	41.0
24	37.21862530067362	39.0	35.0	41.0	32.0	41.0
25	37.060678866620904	39.0	35.0	41.0	31.0	41.0
26	37.05294367148303	39.0	35.0	41.0	32.0	41.0
27	36.776042102678296	39.0	35.0	41.0	31.0	41.0
28	36.74503156026588	39.0	35.0	41.0	31.0	41.0
29	36.69396587807859	39.0	35.0	40.0	31.0	41.0
30	36.53891037554205	39.0	35.0	40.0	30.0	41.0
31	36.499870242938705	39.0	35.0	40.0	30.0	41.0
32	36.365613541614344	38.0	35.0	40.0	30.0	41.0
33	36.20886980204374	38.0	35.0	40.0	30.0	41.0
34	36.071422193201286	38.0	35.0	40.0	30.0	41.0
35	35.98873066898834	38.0	35.0	40.0	29.0	41.0
36	35.896347827057554	38.0	35.0	40.0	29.0	41.0
37	35.84802936695297	38.0	35.0	40.0	29.0	41.0
38	35.7220826985004	38.0	35.0	40.0	29.0	41.0
39	35.662783721488324	38.0	34.0	40.0	28.0	41.0
40	35.53647708183345	38.0	34.0	40.0	28.0	41.0
41	35.45713133646983	38.0	34.0	40.0	28.0	41.0
42	35.4201129025957	38.0	34.0	40.0	28.0	41.0
43	35.33637215999464	38.0	34.0	40.0	27.0	41.0
44	35.25012696658686	37.0	34.0	40.0	27.0	41.0
45	35.186843192079515	37.0	34.0	40.0	27.0	41.0
46	35.02697412114008	37.0	34.0	40.0	27.0	41.0
47	34.914317087191165	37.0	34.0	40.0	26.0	41.0
48	34.8327333813295	36.0	34.0	40.0	26.0	41.0
49	34.76313476317244	36.0	34.0	40.0	26.0	41.0
50	34.61928999168438	36.0	34.0	40.0	26.0	41.0
51	33.874284940925655	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	25.0
10	36.0
11	51.0
12	32.0
13	37.0
14	33.0
15	58.0
16	93.0
17	194.0
18	360.0
19	570.0
20	1135.0
21	1973.0
22	2942.0
23	3954.0
24	5148.0
25	6246.0
26	7459.0
27	8646.0
28	10455.0
29	12635.0
30	15667.0
31	19937.0
32	25286.0
33	34029.0
34	54981.0
35	68439.0
36	66787.0
37	91282.0
38	129200.0
39	148962.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.387624803969175	20.058209296744632	20.754711716085968	13.799454183200227
2	28.278946986566655	28.07384711548658	25.122641351482578	18.52456454646419
3	31.375536468710408	26.905335945217406	25.470194942544133	16.248932643528054
4	28.722492898242557	26.18092878151143	26.776974121140075	18.31960419910593
5	26.10684168522332	29.965928307130778	24.131325307928854	19.795904699717045
6	24.870103414982616	36.39713474084864	23.26488299540688	15.467878848761865
7	78.23555510907964	7.130917898661131	9.646391079411321	4.9871359128479025
8	79.08274314799002	7.598601414212444	7.503446235929033	5.815209201868502
9	73.2897182178914	8.324124767693004	10.146304574703791	8.239852439711798
10	41.20358743393552	26.809064577159408	16.377433991327205	15.609913997577868
11	31.56473063550265	26.90017356750995	22.03023758099352	19.504858215993885
12	27.24423906552592	22.19194557458659	29.288680161401043	21.275135198486446
13	26.30873251070147	24.292056635469162	29.914862624943495	19.484348228885874
14	19.927475569396307	29.875656459111177	27.272422857334206	22.92444511415831
15	18.786869143491778	25.514284438640257	33.863244428817794	21.83560198905018
16	21.693427316512352	24.32833280314319	30.206048632388477	23.77219124795598
17	21.852623883112607	23.724753182536094	27.364648037459325	27.057974896891967
18	23.708149859639136	23.5262109263817	28.44093402760337	24.324705186375787
19	24.656632120593144	26.08535503206255	25.52488824149882	23.733124605845486
20	26.18818401504624	25.158359424269317	25.42331497201154	23.230141588672907
21	25.626461510986097	27.6587640430626	25.7991918786032	20.9155825673481
22	22.315842639565577	25.920437992867544	24.846802953438143	26.91691641412873
23	22.05911899141092	29.185432607251887	25.282675060413773	23.472773340923425
24	22.38058164649154	25.672085768022278	26.51941333065448	25.427919254831703
25	21.287692333450533	27.630719774976143	26.271898248140147	24.809689643433178
26	21.691194936963182	26.07879741713686	26.960866386503035	25.26914125939692
27	24.05612202186616	25.888487060570036	25.76765951747116	24.287731400092643
28	21.088312934965202	27.862189629480806	27.464826069728375	23.584671365825617
29	23.905157354853472	26.26338730110894	25.603579620607096	24.227875723430497
30	24.63151785066497	27.216194797439456	24.946422890819896	23.205864461075674
31	25.049251873803584	28.03073428544321	21.726773486028094	25.19324035472511
32	26.091633599544593	27.335487579598283	22.800548049179323	23.7723307716778
33	25.5530720333071	26.293245377579094	22.49415395605561	25.659528633058194
34	24.10718770405344	26.796088871029855	24.583940261523267	24.51278316339344
35	24.680769724468554	24.73057969315943	24.92563385626824	25.66301672610377
36	25.172590843895282	28.668078646731516	23.15577544494115	23.003555064432053
37	23.253442050217377	28.040779993414482	24.81596821091522	23.889809745452922
38	22.564613435576316	29.760828436050694	23.690569870689416	23.98398825768357
39	23.393244819484206	27.32167473113779	23.431892890429232	25.853187558948772
40	24.20038955023133	24.97823429939558	27.970739085059243	22.850637065313844
41	21.824021520138853	25.548607274208763	25.34253073707592	27.284840468576466
42	23.644108471322294	24.728905408497553	26.570618536563583	25.056367583616566
43	23.58788041142755	23.830791211121717	26.325614881042075	26.25571349640866
44	23.326133909287257	24.894799113745318	26.26882872626004	25.510238250707385
45	22.776829016469378	25.1769160792718	25.764729519312873	26.281525384945947
46	23.864834999246572	27.054905375011863	25.179148458820972	23.9011111669206
47	22.303704075766962	26.01377936276726	27.53319269342174	24.149323868044046
48	22.304262170654255	24.7676930031644	27.14740960258063	25.78063522360072
49	22.3045412180979	23.858416908042706	28.545437295248938	25.291604578610453
50	21.79011725573582	23.992220157271138	28.299038402509197	25.918624184483846
51	22.159157499958145	23.86748594996121	25.425965922726178	28.547390627354464
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1734.0
1	1863.0
2	1992.0
3	2466.0
4	2940.0
5	2168.5
6	1397.0
7	1456.0
8	1515.0
9	1541.0
10	1567.0
11	1610.5
12	1654.0
13	1692.5
14	1731.0
15	1758.0
16	1785.0
17	1875.5
18	1966.0
19	2064.5
20	2163.0
21	2072.5
22	1982.0
23	2210.0
24	2438.0
25	3135.0
26	4673.5
27	5515.0
28	6147.0
29	6779.0
30	8231.5
31	9684.0
32	10430.5
33	11177.0
34	13024.0
35	14871.0
36	14922.5
37	14974.0
38	16329.0
39	17684.0
40	20524.0
41	23364.0
42	27403.5
43	31443.0
44	37156.5
45	42870.0
46	54130.0
47	65390.0
48	66470.0
49	67550.0
50	67373.5
51	67197.0
52	59150.0
53	51103.0
54	46735.0
55	42367.0
56	40296.0
57	38225.0
58	37132.5
59	36040.0
60	34761.5
61	33483.0
62	30724.0
63	27965.0
64	25284.5
65	22604.0
66	19761.5
67	16919.0
68	14413.0
69	11907.0
70	10766.5
71	9626.0
72	8680.0
73	7734.0
74	6224.0
75	3894.0
76	3074.0
77	2342.0
78	1610.0
79	1337.0
80	1064.0
81	856.0
82	648.0
83	450.5
84	253.0
85	207.0
86	161.0
87	92.0
88	23.0
89	14.5
90	6.0
91	3.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	716724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.250249811040877
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37448929620757	20.812239125123238
2	9.723818909297718	5.2995298879137165
3	3.518935605101328	2.8767562292393247
4	1.7130069695200885	1.8671947138990599
5	0.9968810709974062	1.3582629108288649
6	0.696148245247738	1.1382128153111168
7	0.5027996722751863	0.9590991672085818
8	0.38359168470200233	0.8362375386854072
9	0.30036707849788996	0.7366570131672023
>10	4.187191733672538	29.218349333028627
>50	1.4844097637627587	26.951860333139777
>100	0.10853971113863486	4.662938456248274
>500	0.005168557673268327	0.8640809324035147
>1k	0.004651701905941494	2.418581543803285
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGC	2786	0.38871308899939166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCG	2782	0.38815499411209897	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTC	2483	0.3464374012869668	RNA PCR Primer, Index 36 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	2260	0.31532361132039666	RNA PCR Primer, Index 15 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGC	1914	0.26704840356957493	RNA PCR Primer, Index 36 (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1352	0.18863607190494527	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1329	0.18542702630301203	No Hit
CTGTCTCTTATACACATCTGACGCGGACAATCTCGTATGCCGTCTTCTGCT	1155	0.16114989870577795	RNA PCR Primer, Index 36 (96% over 26bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1111	0.15501085494555783	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	757	0.10561945742015057	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.7904744364636876E-4	0.0	0.0	0.17454417600080366	0.0
2	2.7904744364636876E-4	0.0	0.0	0.9578303503161607	0.0
3	2.7904744364636876E-4	0.0	0.0	1.3034306092721886	0.0
4	2.7904744364636876E-4	0.0	0.0	1.694236554098928	0.0
5	2.7904744364636876E-4	0.0	0.0	3.092124723045412	0.0
6	2.7904744364636876E-4	0.0	0.0	3.9715427416969433	0.0
7	2.7904744364636876E-4	0.0	0.0	4.8541698059504075	0.0
8	2.7904744364636876E-4	0.0	0.0	6.30103080125683	0.0
9	2.7904744364636876E-4	0.0	0.0	6.985673704243196	0.0
10	2.7904744364636876E-4	0.0	0.0	8.65354027491754	0.0
11	2.7904744364636876E-4	0.0	0.0	9.978597061072323	0.0
12	2.7904744364636876E-4	0.0	0.0	11.536100367784531	0.0
13	2.7904744364636876E-4	0.0	0.0	12.050245282702965	0.0
14	2.7904744364636876E-4	0.0	0.0	12.263018958483322	0.0
15	2.7904744364636876E-4	0.0	0.0	12.633733487367522	0.0
16	2.7904744364636876E-4	0.0	0.0	13.311818775428199	0.0
17	2.7904744364636876E-4	0.0	0.0	14.13124159369576	0.0
18	2.7904744364636876E-4	0.0	0.0	15.224689001624055	0.0
19	2.7904744364636876E-4	0.0	0.0	15.759622951094146	0.0
20	2.7904744364636876E-4	0.0	0.0	16.277674530223628	0.0
21	2.7904744364636876E-4	0.0	0.0	16.758194228182674	0.0
22	2.7904744364636876E-4	0.0	0.0	17.263130577460778	0.0
23	8.371423309391062E-4	0.0	0.0	17.76262550158778	0.0
24	8.371423309391062E-4	0.0	0.0	18.146315736601537	0.0
25	8.371423309391062E-4	0.0	0.0	18.50210122725066	0.0
26	8.371423309391062E-4	0.0	0.0	18.827191499098678	0.0
27	8.371423309391062E-4	0.0	0.0	19.21478839832348	0.0
28	9.766660527622906E-4	0.0	0.0	19.54727342742813	0.0
29	9.766660527622906E-4	0.0	0.0	19.874596078825324	0.0
30	9.766660527622906E-4	0.0	0.0	20.28981867497112	0.0
31	9.766660527622906E-4	0.0	0.0	20.64699940283847	0.0
32	9.766660527622906E-4	0.0	0.0	21.005435844202232	0.0
33	9.766660527622906E-4	0.0	0.0	21.37684799169555	0.0
34	9.766660527622906E-4	0.0	0.0	21.744632522421462	0.0
35	9.766660527622906E-4	0.0	0.0	22.13655465702279	0.0
36	9.766660527622906E-4	0.0	0.0	22.464714450750918	0.0
37	9.766660527622906E-4	0.0	0.0	22.811709946925177	0.0
38	9.766660527622906E-4	0.0	0.0	23.149636401180928	0.0
39	9.766660527622906E-4	0.0	0.0	23.49453904152784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	35	1.211065E-7	45.000004	1
GTCGATC	20	7.0315064E-4	45.000004	8
AGTCAAC	20	7.0315064E-4	45.000004	37
CGAAATA	20	7.0315064E-4	45.000004	28
CGATTGT	40	6.8084773E-9	45.000004	10
TCGATGA	20	7.0315064E-4	45.000004	20
GCTTGAC	20	7.0315064E-4	45.000004	26
CGTTAGC	20	7.0315064E-4	45.000004	31
GCCGATA	30	2.1643536E-6	45.000004	9
CTAACGG	20	7.0315064E-4	45.000004	2
TCGGAAC	20	7.0315064E-4	45.000004	34
GTTAACG	20	7.0315064E-4	45.000004	1
TGTTACG	35	1.211065E-7	45.000004	26
TTACGAA	20	7.0315064E-4	45.000004	24
CGGTCGA	20	7.0315064E-4	45.000004	6
CGTCCAT	20	7.0315064E-4	45.000004	20
GACTAGT	20	7.0315064E-4	45.000004	2
TGCGTAT	30	2.1643536E-6	45.000004	15
ATTAGCG	45	3.8380676E-10	45.0	1
AACCGCT	25	3.889332E-5	44.999996	45
>>END_MODULE