##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552649_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574481
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82959575686576	31.0	31.0	34.0	30.0	34.0
2	31.97872862636014	33.0	31.0	34.0	30.0	34.0
3	32.01048598648171	33.0	31.0	34.0	30.0	34.0
4	35.6418750141432	37.0	35.0	37.0	33.0	37.0
5	35.44882076169621	37.0	35.0	37.0	33.0	37.0
6	35.447243686040096	37.0	35.0	37.0	33.0	37.0
7	35.762987809866644	37.0	35.0	37.0	35.0	37.0
8	35.54966482790554	37.0	35.0	37.0	35.0	37.0
9	37.24955220451155	39.0	37.0	39.0	34.0	39.0
10	36.86678062459855	39.0	37.0	39.0	32.0	39.0
11	36.81177097240814	39.0	37.0	39.0	32.0	39.0
12	36.69053110546737	39.0	35.0	39.0	33.0	39.0
13	36.498820674661125	39.0	35.0	39.0	32.0	39.0
14	37.63230811811009	40.0	36.0	41.0	33.0	41.0
15	37.77817543138938	40.0	36.0	41.0	33.0	41.0
16	37.85203862268726	40.0	36.0	41.0	33.0	41.0
17	37.82758350580785	40.0	36.0	41.0	33.0	41.0
18	37.78179609073233	40.0	36.0	41.0	33.0	41.0
19	37.74687761649211	40.0	36.0	41.0	33.0	41.0
20	37.681484679214805	39.0	36.0	41.0	33.0	41.0
21	37.56359914427109	39.0	35.0	41.0	33.0	41.0
22	37.56666451980135	39.0	35.0	41.0	33.0	41.0
23	37.500678003276	39.0	35.0	41.0	33.0	41.0
24	37.491673353862005	39.0	35.0	41.0	33.0	41.0
25	37.348023694430275	39.0	35.0	41.0	32.0	41.0
26	37.315422094029216	39.0	35.0	41.0	32.0	41.0
27	37.09840708395926	39.0	35.0	41.0	32.0	41.0
28	37.09796494575104	39.0	35.0	41.0	32.0	41.0
29	37.07527490030132	39.0	35.0	41.0	32.0	41.0
30	36.96799023814539	39.0	35.0	41.0	31.0	41.0
31	36.94379796720866	39.0	35.0	41.0	31.0	41.0
32	36.827647911767315	39.0	35.0	41.0	31.0	41.0
33	36.728499288923395	39.0	35.0	41.0	31.0	41.0
34	36.61149977109774	39.0	35.0	41.0	31.0	41.0
35	36.487123159860815	39.0	35.0	41.0	30.0	41.0
36	36.434252829945635	39.0	35.0	40.0	30.0	41.0
37	36.34832483580832	38.0	35.0	40.0	30.0	41.0
38	36.23710270661693	38.0	35.0	40.0	30.0	41.0
39	36.219681764932176	38.0	35.0	40.0	30.0	41.0
40	36.101005951458795	38.0	35.0	40.0	30.0	41.0
41	36.016740327356345	38.0	35.0	40.0	30.0	41.0
42	35.964242507585105	38.0	35.0	40.0	30.0	41.0
43	35.8983621738578	38.0	35.0	40.0	30.0	41.0
44	35.836020686497896	38.0	35.0	40.0	30.0	41.0
45	35.79255188596316	38.0	35.0	40.0	30.0	41.0
46	35.68975649325217	38.0	35.0	40.0	29.0	41.0
47	35.60150118106604	37.0	34.0	40.0	29.0	41.0
48	35.51967602061687	37.0	34.0	40.0	29.0	41.0
49	35.45262593540953	37.0	34.0	40.0	29.0	41.0
50	35.36080044422705	37.0	34.0	40.0	29.0	41.0
51	34.68543607186312	36.0	33.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	17.0
10	41.0
11	32.0
12	31.0
13	37.0
14	40.0
15	59.0
16	80.0
17	163.0
18	260.0
19	409.0
20	678.0
21	1033.0
22	1497.0
23	2148.0
24	2855.0
25	3745.0
26	4748.0
27	5729.0
28	7046.0
29	8536.0
30	10844.0
31	13764.0
32	17961.0
33	25000.0
34	42257.0
35	55151.0
36	51670.0
37	72430.0
38	107516.0
39	138605.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92392785836259	20.167420680579514	24.144749782847473	13.763901678210418
2	29.509243995884983	25.687533617299792	26.75997987748942	18.043242509325808
3	31.99809915384495	24.48331624544589	26.52811842341174	16.99046617729742
4	30.177499342885145	26.31000851203086	24.755561976810373	18.756930168273627
5	24.981853185745045	30.89240549295799	24.136394415132965	19.989346906164
6	25.115190928855785	36.56709273239672	23.345941815308077	14.971774523439418
7	81.34716378783634	7.16507595551463	6.6668871555369105	4.820873101112134
8	82.93799098664708	5.982443283589884	6.2708775399012335	4.808688189861806
9	77.25512244965455	8.25127375840106	8.636142883750725	5.857460908193657
10	42.0818094941347	26.778953525007786	15.885991007535496	15.253245973322008
11	32.18139503308203	26.955460667976833	21.08859997110435	19.774544327836775
12	28.231743086368393	23.604261933815042	28.077865064292812	20.086129915523752
13	24.9585277842087	25.79110536292758	28.53741028859092	20.712956564272798
14	20.79233255756065	28.601119967414068	28.181959020402765	22.424588454622523
15	19.686464826512974	26.159960033491096	32.1692101218317	21.98436501816422
16	23.2058153359293	23.225485264090544	30.395609254266027	23.17309014571413
17	22.76733260107819	23.336542026629253	27.05520287006881	26.840922502223748
18	24.876192598188627	24.115157855525247	27.652959801977783	23.355689744308343
19	26.134893930347562	26.820034082937465	24.697422543130234	22.34764944358473
20	27.202118085715625	25.038251917817995	24.67096387870095	23.088666117765428
21	26.203477573670842	26.51245210894703	25.07184745883676	22.212222858545367
22	23.487460855972607	24.948431714887	24.992993676031062	26.571113753109326
23	22.700663729522823	26.834307836116427	25.217021972876385	25.24800646148437
24	22.505531079356846	26.19094452209908	27.986826370236788	23.31669802830729
25	23.29093564452088	26.948671931708795	25.426950586703477	24.33344183706685
26	22.23537418992099	27.761405512105707	25.2419140058592	24.7613062921141
27	22.94296939324364	26.084065443417625	26.392169627890215	24.58079553544852
28	21.861471484696622	27.022303609692923	28.025295875755685	23.090929029854774
29	23.339153079040038	26.597746487699332	26.29277556611968	23.77032486714095
30	23.636987124030213	27.509351919384628	25.558373558046306	23.295287398538854
31	26.245428482404115	27.363829265023558	22.98039447779822	23.410347774774102
32	25.210407306769067	28.04444359343477	22.6463538393785	24.098795260417663
33	25.880751495697858	27.229621171109226	22.48899441408854	24.400632919104375
34	23.52854141390229	27.05346216846162	25.073065949961794	24.3449304676743
35	24.012108320379614	26.150560244812276	24.11498378536453	25.722347649443584
36	26.31279363460236	27.817456103857218	23.680156523888517	22.189593737651897
37	25.705288773693123	27.282364429807078	25.383607116684452	21.628739679815347
38	24.045181650916216	28.463256400124635	24.509600839714455	22.981961109244693
39	24.213333426170752	26.785045980632955	25.371248135273405	23.630372457922892
40	23.935169309341823	26.93109084547618	27.04023283624698	22.09350700893502
41	22.37201926608539	26.443520325302316	25.714514492211233	25.469945916401066
42	23.692863645621003	26.727602827595693	25.086469352337154	24.493064174446154
43	24.410728988426076	23.71253357378225	27.08618735867679	24.790550079114887
44	24.291839068655012	24.45076512539144	26.58973926030626	24.667656545647286
45	23.32522746618252	25.730877087318817	26.13054217632959	24.813353270169074
46	23.810535074267033	26.991319121084945	26.022096466201667	23.176049338446354
47	23.379189216005404	27.439549784936318	26.085458004703376	23.095802994354905
48	22.78439147682865	25.621909166708733	26.572854454716516	25.0208449017461
49	23.104506502390855	25.0144043057995	27.57549858045784	24.305590611351814
50	22.28481011556518	25.386566309416676	27.482196974312465	24.84642660070568
51	21.323942828396415	26.269450164583336	26.182937294706004	26.223669712314248
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1517.0
1	1677.5
2	1838.0
3	2135.0
4	2432.0
5	1799.0
6	1166.0
7	1167.0
8	1168.0
9	1211.5
10	1255.0
11	1288.5
12	1322.0
13	1410.0
14	1498.0
15	1424.0
16	1350.0
17	1261.0
18	1172.0
19	1331.5
20	1491.0
21	1465.0
22	1439.0
23	1606.0
24	1773.0
25	2546.5
26	3416.0
27	3512.0
28	4933.5
29	6355.0
30	6797.0
31	7239.0
32	8435.0
33	9631.0
34	11341.5
35	13052.0
36	14286.0
37	15520.0
38	16530.0
39	17540.0
40	18968.0
41	20396.0
42	24379.0
43	28362.0
44	32365.5
45	36369.0
46	39586.5
47	42804.0
48	45686.5
49	48569.0
50	48203.0
51	47837.0
52	43927.0
53	40017.0
54	37660.5
55	35304.0
56	33051.0
57	30798.0
58	29667.5
59	28537.0
60	28107.0
61	27677.0
62	25522.0
63	23367.0
64	20332.0
65	17297.0
66	15627.0
67	13957.0
68	13034.5
69	12112.0
70	9880.0
71	7648.0
72	6938.0
73	6228.0
74	5270.0
75	3848.0
76	3384.0
77	2686.5
78	1989.0
79	1489.5
80	990.0
81	757.0
82	524.0
83	360.0
84	196.0
85	161.5
86	127.0
87	95.5
88	64.0
89	44.0
90	24.0
91	12.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	574481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.844867962628328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24263555981045	19.96325717128721
2	8.9064193992177	4.60370466785146
3	2.9471963992230106	2.2850970539355706
4	1.4579217968636176	1.5071918535911213
5	0.8918832025896964	1.1525301804508397
6	0.6008297980510072	0.9317020079184555
7	0.42292572369869313	0.7651321640894228
8	0.3703117575863308	0.7656526783862038
9	0.27729330574801475	0.6449947986580351
>10	5.1853310882872234	36.02776752708055
>50	1.6213365453941062	26.89094170152751
>100	0.06913726071496608	3.0379840980553605
>500	0.004744713970634927	0.8382397594968803
>1k	0.002033448844557826	0.5858043376713831
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1178	0.20505464932695774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1165	0.20279173723761096	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1001	0.17424423087969837	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	873	0.151963250307669	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	796	0.1385598479323076	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	704	0.12254539314616149	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	637	0.11088269237798988	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	637	0.11088269237798988	No Hit
GCTAAAGGTGTAATTTGAAATGGCCTTCGGGTAAATGCAAGATACTTAACT	626	0.10896792061008109	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.481403214379588E-4	0.0	0.0	0.09765336016334744	0.0
2	3.481403214379588E-4	0.0	0.0	0.4779966613343174	0.0
3	3.481403214379588E-4	0.0	0.0	0.6484113486781983	0.0
4	3.481403214379588E-4	0.0	0.0	0.8541622786480318	0.0
5	3.481403214379588E-4	0.0	0.0	1.537909869952183	0.0
6	3.481403214379588E-4	0.0	0.0	2.128007714789523	0.0
7	3.481403214379588E-4	0.0	0.0	2.6488256356607094	0.0
8	3.481403214379588E-4	0.0	0.0	3.5698308560248293	0.0
9	3.481403214379588E-4	0.0	0.0	4.015798607786855	0.0
10	3.481403214379588E-4	0.0	0.0	4.871179377559919	0.0
11	3.481403214379588E-4	0.0	0.0	5.8569386977115	0.0
12	3.481403214379588E-4	0.0	0.0	6.689516276430378	0.0
13	3.481403214379588E-4	0.0	0.0	7.012242354403366	0.0
14	3.481403214379588E-4	0.0	0.0	7.15132441281783	0.0
15	3.481403214379588E-4	0.0	0.0	7.372915727413091	0.0
16	3.481403214379588E-4	0.0	0.0	7.853349370997474	0.0
17	3.481403214379588E-4	0.0	0.0	8.428651252173701	0.0
18	3.481403214379588E-4	0.0	0.0	9.103347195120465	0.0
19	3.481403214379588E-4	0.0	0.0	9.42137337875404	0.0
20	3.481403214379588E-4	0.0	0.0	9.729303493065915	0.0
21	3.481403214379588E-4	0.0	0.0	10.068218095985769	0.0
22	3.481403214379588E-4	0.0	0.0	10.418447259352355	0.0
23	3.481403214379588E-4	0.0	0.0	10.779990983165675	0.0
24	3.481403214379588E-4	0.0	0.0	11.09140250069193	0.0
25	3.481403214379588E-4	0.0	0.0	11.344848654698763	0.0
26	3.481403214379588E-4	0.0	0.0	11.563828916883239	0.0
27	3.481403214379588E-4	0.0	0.0	11.825978578926023	0.0
28	3.481403214379588E-4	0.0	0.0	12.08917266193312	0.0
29	3.481403214379588E-4	0.0	0.0	12.348537201404397	0.0
30	3.481403214379588E-4	0.0	0.0	12.664300472948627	0.0
31	3.481403214379588E-4	0.0	0.0	12.924187222902063	0.0
32	3.481403214379588E-4	0.0	0.0	13.217495443713544	0.0
33	3.481403214379588E-4	0.0	0.0	13.487304192827962	0.0
34	3.481403214379588E-4	0.0	0.0	13.739357785549043	0.0
35	3.481403214379588E-4	0.0	0.0	14.033014146681962	0.0
36	3.481403214379588E-4	0.0	0.0	14.288375072456704	0.0
37	3.481403214379588E-4	0.0	0.0	14.555920909481776	0.0
38	3.481403214379588E-4	0.0	0.0	14.837740499685804	0.0
39	3.481403214379588E-4	0.0	0.0	15.104938196389437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAT	30	2.1635988E-6	45.000004	17
AATTCGG	30	2.1635988E-6	45.000004	2
TATCAGC	20	7.030339E-4	45.0	35
AACGTGA	20	7.030339E-4	45.0	32
CTATCGC	25	3.8883612E-5	45.0	41
GTTAGCG	35	1.210501E-7	45.0	1
CGATTGT	20	7.030339E-4	45.0	10
TCGATAG	40	6.8048394E-9	45.0	1
ATATCGT	25	3.8883612E-5	45.0	11
TCGGAAC	25	3.8883612E-5	45.0	43
CGGATGT	20	7.030339E-4	45.0	25
TATTACG	35	1.210501E-7	45.0	1
CGAATGT	20	7.030339E-4	45.0	29
TTACGTG	25	3.8883612E-5	45.0	36
ACGTAAC	20	7.030339E-4	45.0	12
GCTAATT	20	7.030339E-4	45.0	40
TTCGCTA	20	7.030339E-4	45.0	19
TTATTCG	20	7.030339E-4	45.0	32
TATCGCG	20	7.030339E-4	45.0	1
TACCGTC	25	3.8883612E-5	45.0	22
>>END_MODULE