FastQCFastQC Report
Sat 18 Jun 2016
SRR3552644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493118
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13490.2735653535259309No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC10270.20826658122396666Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8400.17034462339642842No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6720.13627569871714276No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC5710.11579378566590552No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.0293626E-445.0000041
AGCCGAT207.0293626E-445.00000424
TCACCGA207.0293626E-445.00000437
GTCGAAC207.0293626E-445.00000436
GTTTACG207.0293626E-445.0000041
CGTTGGA207.0293626E-445.00000432
TAGCGAC207.0293626E-445.00000440
TAACGAT207.0293626E-445.00000434
CGACAAT207.0293626E-445.00000419
CCGTCTA207.0293626E-445.00000420
TCGGACA302.1629712E-645.00000427
CTAGTCG207.0293626E-445.0000041
TGCGATG207.0293626E-445.0000041
CCTCACG207.0293626E-445.00000412
GTCCGAA207.0293626E-445.0000041
AGCGAAT207.0293626E-445.00000416
AATTCGC302.1629712E-645.00000413
ACGCTCA207.0293626E-445.00000445
CATCGGT207.0293626E-445.00000431
GCTACGA302.1629712E-645.00000410