##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493118
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.780283826589173	31.0	31.0	34.0	30.0	34.0
2	31.919869077989446	33.0	31.0	34.0	30.0	34.0
3	31.96312444485904	33.0	31.0	34.0	30.0	34.0
4	35.60053171857446	37.0	35.0	37.0	33.0	37.0
5	35.41972306831225	37.0	35.0	37.0	33.0	37.0
6	35.436664246691464	37.0	35.0	37.0	33.0	37.0
7	35.80500407610349	37.0	35.0	37.0	35.0	37.0
8	35.62053098852607	37.0	35.0	37.0	35.0	37.0
9	37.3233161231186	39.0	37.0	39.0	34.0	39.0
10	36.81813075166593	39.0	37.0	39.0	32.0	39.0
11	36.806237046710926	39.0	37.0	39.0	32.0	39.0
12	36.73551563723085	39.0	35.0	39.0	33.0	39.0
13	36.55745683588918	39.0	35.0	39.0	32.0	39.0
14	37.712947002543004	40.0	36.0	41.0	33.0	41.0
15	37.8527715475809	40.0	37.0	41.0	33.0	41.0
16	37.91830352978395	40.0	37.0	41.0	33.0	41.0
17	37.90090809907568	40.0	37.0	41.0	33.0	41.0
18	37.85392745752538	40.0	36.0	41.0	33.0	41.0
19	37.791106793911396	40.0	36.0	41.0	33.0	41.0
20	37.736912868725135	40.0	36.0	41.0	33.0	41.0
21	37.640808082446796	39.0	36.0	41.0	33.0	41.0
22	37.683438852363935	39.0	36.0	41.0	33.0	41.0
23	37.589522183331376	39.0	36.0	41.0	33.0	41.0
24	37.61892690998909	39.0	36.0	41.0	33.0	41.0
25	37.47502220563841	39.0	35.0	41.0	32.0	41.0
26	37.48044687072871	39.0	35.0	41.0	33.0	41.0
27	37.24750465405846	39.0	35.0	41.0	32.0	41.0
28	37.28476956833861	39.0	35.0	41.0	32.0	41.0
29	37.276698477849116	39.0	35.0	41.0	32.0	41.0
30	37.16427913805621	39.0	35.0	41.0	32.0	41.0
31	37.12538783820506	39.0	35.0	41.0	32.0	41.0
32	37.0009348675165	39.0	35.0	41.0	31.0	41.0
33	36.915738220872086	39.0	35.0	41.0	31.0	41.0
34	36.82855016446368	39.0	35.0	41.0	31.0	41.0
35	36.72776901269067	39.0	35.0	41.0	31.0	41.0
36	36.712060804918906	39.0	35.0	41.0	31.0	41.0
37	36.660409881610484	39.0	35.0	41.0	31.0	41.0
38	36.63171086839255	39.0	35.0	41.0	31.0	41.0
39	36.547305107499625	39.0	35.0	41.0	30.0	41.0
40	36.49629500444113	39.0	35.0	41.0	30.0	41.0
41	36.40415073065676	39.0	35.0	41.0	30.0	41.0
42	36.369769507501246	39.0	35.0	40.0	30.0	41.0
43	36.31674365973256	39.0	35.0	40.0	30.0	41.0
44	36.277239930402054	39.0	35.0	40.0	30.0	41.0
45	36.23895092046934	39.0	35.0	40.0	30.0	41.0
46	36.15652845769167	38.0	35.0	40.0	30.0	41.0
47	36.06638370532003	38.0	35.0	40.0	30.0	41.0
48	36.016697828917216	38.0	35.0	40.0	30.0	41.0
49	35.962708722861464	38.0	35.0	40.0	29.0	41.0
50	35.874579715200014	38.0	35.0	40.0	29.0	41.0
51	35.114838639027575	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	10.0
10	21.0
11	33.0
12	30.0
13	15.0
14	31.0
15	28.0
16	68.0
17	96.0
18	193.0
19	296.0
20	518.0
21	764.0
22	1238.0
23	1820.0
24	2457.0
25	3269.0
26	3916.0
27	4658.0
28	5533.0
29	6791.0
30	8638.0
31	11361.0
32	14806.0
33	20426.0
34	32220.0
35	42959.0
36	41730.0
37	58829.0
38	96807.0
39	133481.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87323520942249	19.911664145295852	23.176197177957405	12.038903467324252
2	31.78792905552018	24.79163202316687	26.68245734286722	16.737981578445726
3	32.16978491963384	24.47588609622849	26.33913181023609	17.01519717390158
4	26.887479264597925	27.98376859088494	25.52411390377151	19.604638240745622
5	24.76932498915067	31.68044970980577	25.80376299384731	17.746462307196246
6	24.036843108546027	36.876163514615165	24.72917232792151	14.357821048917296
7	80.44240932190672	7.401473886574816	7.698360230208591	4.457756561309869
8	82.69562254876115	5.838764758130914	6.409419246508949	5.056193446598988
9	76.80899906310457	8.5024274108834	9.219091576458373	5.469481949553656
10	38.29448529560876	26.75586776390235	18.75697094813006	16.192675992358826
11	29.952465738423662	28.951285493532986	22.40275147125029	18.693497296793062
12	25.270016507205174	24.74458446051452	29.742374036234736	20.243024996045573
13	23.57529029562904	27.49828641420512	30.23353436702777	18.692888923138074
14	21.23872987804136	29.993632355744467	29.471850550983742	19.29578721523043
15	20.021576985630215	28.256117197100895	33.39302154859486	18.32928426867403
16	22.23524592491047	26.032714279340848	30.870096001362757	20.86194379438593
17	23.105625833978884	26.37725655928196	27.651393784043577	22.865723822695582
18	23.843177495041754	25.667081712693513	29.728989815825013	20.760750976439716
19	23.946803807607917	29.13359479881083	26.748364488824176	20.171236904757077
20	24.49292055856813	26.834145174177376	27.20281960909965	21.47011465815484
21	22.6008784915578	29.07559651036872	27.903868850863283	20.4196561472102
22	21.47518443861307	27.300159393897605	26.79155901832827	24.433097149161053
23	20.10735767098342	29.931578242935768	27.452658390081076	22.508405695999738
24	21.236499174639743	27.49362221618355	29.20376056035269	22.066118048824013
25	20.33813407744191	29.132175260282526	27.982754634793295	22.546936027482268
26	20.376258826487778	30.00417749909758	27.868988761310682	21.75057491310396
27	21.79741968453798	29.541610730088948	26.827858646409176	21.833110938963898
28	20.474004193722394	28.60633763115522	29.30414221342559	21.615515961696794
29	21.69379337197182	27.870408299838985	27.272782579423183	23.163015748766018
30	23.286921183165084	29.16705534983513	26.580858942484355	20.96516452451543
31	23.399267518119395	30.020806379000565	24.309191714761987	22.270734388118058
32	22.951707299267113	29.590686204924584	25.8218114122786	21.6357950835297
33	24.626762762665326	28.234621327958013	24.128301948012442	23.01031396136422
34	21.6343755450014	30.581524097680475	25.75103727708175	22.033063080236374
35	23.028565171013835	28.443699074055296	25.269408133550186	23.258327621380683
36	25.31909198204081	29.50510831078971	24.327442924411603	20.84835678275788
37	22.74486025657145	30.34405558101712	26.015071443346216	20.89601271906521
38	23.495998929262367	29.74501032207301	24.7346476908164	22.024343057848224
39	22.787040829983898	28.83021913619053	26.282958642758935	22.099781391066642
40	24.29479353826062	27.80227045048041	26.6885410794171	21.21439493184187
41	21.724009263502854	29.145356689473918	25.526952980828117	23.603681066195108
42	22.414310570695047	28.380428213936625	26.953589201773205	22.251672013595122
43	22.428505955978082	27.049509448042862	28.41713342445419	22.10485117152487
44	21.81283181713099	28.32547179376944	26.816907920619403	23.04478846848016
45	21.837166763330483	28.119030333510437	26.964539927562974	23.079262975596105
46	24.141888959640493	28.167497434691086	25.71047903341594	21.98013457225248
47	21.43766806322219	28.287549835941906	27.51430692045312	22.760475180382787
48	22.47737863959539	26.768035237002096	27.52262136040461	23.231964762997904
49	22.089439038931857	25.868047810057632	28.797164167602883	23.245348983407624
50	22.247210606791885	26.56869146938461	27.648149124550308	23.535948799273196
51	21.10691558612746	26.484330322559714	26.19738074862406	26.21137334268877
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1611.0
1	1628.0
2	1645.0
3	1784.5
4	1924.0
5	1463.5
6	1003.0
7	1049.5
8	1096.0
9	1203.5
10	1311.0
11	1330.0
12	1349.0
13	1394.5
14	1440.0
15	1337.0
16	1234.0
17	1305.5
18	1377.0
19	1262.0
20	1147.0
21	1359.5
22	1572.0
23	1649.5
24	1727.0
25	2230.0
26	3479.5
27	4226.0
28	4459.0
29	4692.0
30	5596.0
31	6500.0
32	7424.5
33	8349.0
34	10446.5
35	12544.0
36	14620.5
37	16697.0
38	17719.0
39	18741.0
40	20686.0
41	22631.0
42	26991.5
43	31352.0
44	35354.5
45	39357.0
46	43539.0
47	47721.0
48	49153.0
49	50585.0
50	49492.5
51	48400.0
52	44254.0
53	40108.0
54	34819.0
55	29530.0
56	26332.5
57	23135.0
58	21522.5
59	19910.0
60	17075.5
61	14241.0
62	12823.0
63	11405.0
64	9296.5
65	7188.0
66	6032.5
67	4877.0
68	4141.5
69	3406.0
70	2955.0
71	2504.0
72	2081.5
73	1659.0
74	1273.5
75	677.0
76	466.0
77	423.0
78	380.0
79	261.0
80	142.0
81	170.5
82	199.0
83	131.5
84	64.0
85	43.5
86	23.0
87	21.5
88	20.0
89	12.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493118.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.70711722174795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.54095912410193	23.503522040888164
2	9.420008505341732	5.785226108067825
3	3.270646389628703	3.012963662316459
4	1.6502135038583443	2.0269319801555836
5	1.0268358323140447	1.5765584135179245
6	0.7329286317790793	1.3503675246729316
7	0.5495895491322025	1.1813417496335104
8	0.43714565162243385	1.0738786213877973
9	0.3850553960098675	1.064154706392742
>10	4.984760133252206	37.31140925148006
>50	0.9513322972229602	18.75347412337599
>100	0.04720097351700222	2.449904130568065
>500	0.001994407331704319	0.4252270897402949
>1k	0.0013296048878028795	0.48504059780265996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1349	0.2735653535259309	No Hit
GCTGTCTCTTATACACATCTGACGCAGACGAGATCGTATGCCGTCTTCTGC	1027	0.20826658122396666	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	840	0.17034462339642842	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	672	0.13627569871714276	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	571	0.11579378566590552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.0558243665816295E-4	0.0	0.0	0.14073710552038254	0.0
2	4.0558243665816295E-4	0.0	0.0	0.7274121001464152	0.0
3	4.0558243665816295E-4	0.0	0.0	0.9910406839742212	0.0
4	4.0558243665816295E-4	0.0	0.0	1.336596920006976	0.0
5	4.0558243665816295E-4	0.0	0.0	2.3177008342830723	0.0
6	4.0558243665816295E-4	0.0	0.0	3.1779411824350356	0.0
7	4.0558243665816295E-4	0.0	0.0	3.9408417457890406	0.0
8	4.0558243665816295E-4	0.0	0.0	5.4143227381681465	0.0
9	4.0558243665816295E-4	0.0	0.0	6.154916267505952	0.0
10	4.0558243665816295E-4	0.0	0.0	7.520106749297328	0.0
11	4.0558243665816295E-4	0.0	0.0	8.816145425638489	0.0
12	4.0558243665816295E-4	0.0	0.0	9.962726974071115	0.0
13	4.0558243665816295E-4	0.0	0.0	10.408259280740106	0.0
14	4.0558243665816295E-4	0.0	0.0	10.601316520589393	0.0
15	4.0558243665816295E-4	0.0	0.0	10.887252138433396	0.0
16	4.0558243665816295E-4	0.0	0.0	11.475143880369405	0.0
17	4.0558243665816295E-4	0.0	0.0	12.222632311130399	0.0
18	4.0558243665816295E-4	0.0	0.0	13.112682968376738	0.0
19	4.0558243665816295E-4	0.0	0.0	13.574032990075398	0.0
20	4.0558243665816295E-4	0.0	0.0	14.018145758216086	0.0
21	4.0558243665816295E-4	0.0	0.0	14.550675497548253	0.0
22	4.0558243665816295E-4	0.0	0.0	15.070834972562348	0.0
23	4.0558243665816295E-4	0.0	0.0	15.643517373123673	0.0
24	4.0558243665816295E-4	0.0	0.0	16.047680271253533	0.0
25	4.0558243665816295E-4	0.0	0.0	16.420004948105728	0.0
26	4.0558243665816295E-4	0.0	0.0	16.78847659180967	0.0
27	4.0558243665816295E-4	0.0	0.0	17.16323476328181	0.0
28	4.0558243665816295E-4	0.0	0.0	17.53190919820408	0.0
29	4.0558243665816295E-4	0.0	0.0	17.939316755827207	0.0
30	4.0558243665816295E-4	0.0	0.0	18.406953305294067	0.0
31	4.0558243665816295E-4	0.0	0.0	18.813955280480535	0.0
32	4.0558243665816295E-4	0.0	0.0	19.219334925920368	0.0
33	4.0558243665816295E-4	0.0	0.0	19.608896856330535	0.0
34	4.0558243665816295E-4	0.0	0.0	19.98041036830941	0.0
35	4.0558243665816295E-4	0.0	0.0	20.388426299587522	0.0
36	4.0558243665816295E-4	0.0	0.0	20.77798822999769	0.0
37	4.0558243665816295E-4	0.0	0.0	21.189451611987394	0.0
38	4.0558243665816295E-4	0.0	0.0	21.58793635600404	0.0
39	4.0558243665816295E-4	0.0	0.0	21.99656066093714	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.0293626E-4	45.000004	1
AGCCGAT	20	7.0293626E-4	45.000004	24
TCACCGA	20	7.0293626E-4	45.000004	37
GTCGAAC	20	7.0293626E-4	45.000004	36
GTTTACG	20	7.0293626E-4	45.000004	1
CGTTGGA	20	7.0293626E-4	45.000004	32
TAGCGAC	20	7.0293626E-4	45.000004	40
TAACGAT	20	7.0293626E-4	45.000004	34
CGACAAT	20	7.0293626E-4	45.000004	19
CCGTCTA	20	7.0293626E-4	45.000004	20
TCGGACA	30	2.1629712E-6	45.000004	27
CTAGTCG	20	7.0293626E-4	45.000004	1
TGCGATG	20	7.0293626E-4	45.000004	1
CCTCACG	20	7.0293626E-4	45.000004	12
GTCCGAA	20	7.0293626E-4	45.000004	1
AGCGAAT	20	7.0293626E-4	45.000004	16
AATTCGC	30	2.1629712E-6	45.000004	13
ACGCTCA	20	7.0293626E-4	45.000004	45
CATCGGT	20	7.0293626E-4	45.000004	31
GCTACGA	30	2.1629712E-6	45.000004	10
>>END_MODULE