FastQCFastQC Report
Sat 18 Jun 2016
SRR3552643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135168
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.23600031008626038No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC20240.17829959970682752No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC20130.17733058014320346No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG18220.16050487681118566No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16900.1488766420476969No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC15650.13786505609742347No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAA253.890774E-545.00000418
ATTACGA253.890774E-545.00000427
TATGTCG253.890774E-545.00000421
TAATGCG302.1654778E-645.0000041
AAGTACG207.0332515E-445.01
ATTACGC207.0332515E-445.01
ATTTTCG207.0332515E-445.014
TTACGTG207.0332515E-445.01
GTATACG453.8562575E-1045.01
CCAGTCG1000.042.75000428
CGTTCAT750.042.017
TTGTGCG1350.040.01
TTAACGA451.9283107E-840.018
ACCGGTA403.4581717E-739.37541
AATGCGG1150.039.1304322
CTATGCG356.2482195E-638.571431
TGTAACG356.2482195E-638.571431
AGTACGG950.037.8947372
ACGTGCG301.1399128E-437.50000410
TCGTTCA850.037.05882616
Produced by FastQC (version 0.11.5)