##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552643_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1135168
Sequences flagged as poor quality	0
Sequence length	51
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.762118030106556	31.0	31.0	34.0	30.0	34.0
2	31.91640532502678	33.0	31.0	34.0	30.0	34.0
3	31.96692119580538	33.0	31.0	34.0	30.0	34.0
4	35.59478773185995	37.0	35.0	37.0	33.0	37.0
5	35.40255891638947	37.0	35.0	37.0	33.0	37.0
6	35.39290395782827	37.0	35.0	37.0	33.0	37.0
7	35.69800417206969	37.0	35.0	37.0	35.0	37.0
8	35.45163447172577	37.0	35.0	37.0	33.0	37.0
9	37.14060473868185	39.0	37.0	39.0	34.0	39.0
10	36.768429871173254	39.0	37.0	39.0	32.0	39.0
11	36.71225756892372	39.0	37.0	39.0	32.0	39.0
12	36.575975538422504	39.0	35.0	39.0	32.0	39.0
13	36.41243851130405	39.0	35.0	39.0	32.0	39.0
14	37.548076584258894	40.0	36.0	41.0	32.0	41.0
15	37.69655064272425	40.0	36.0	41.0	33.0	41.0
16	37.73790839769973	40.0	36.0	41.0	33.0	41.0
17	37.67282375824548	40.0	36.0	41.0	33.0	41.0
18	37.650141653041665	39.0	36.0	41.0	33.0	41.0
19	37.620223614478206	39.0	36.0	41.0	33.0	41.0
20	37.517291713649435	39.0	36.0	41.0	32.0	41.0
21	37.433913746687715	39.0	35.0	41.0	32.0	41.0
22	37.44999594773637	39.0	35.0	41.0	32.0	41.0
23	37.30913662118735	39.0	35.0	41.0	32.0	41.0
24	37.30800903478604	39.0	35.0	41.0	32.0	41.0
25	37.15900289648757	39.0	35.0	41.0	32.0	41.0
26	37.12169564328804	39.0	35.0	41.0	32.0	41.0
27	36.89792788380222	39.0	35.0	41.0	31.0	41.0
28	36.87496476292496	39.0	35.0	41.0	31.0	41.0
29	36.841288690308396	39.0	35.0	41.0	31.0	41.0
30	36.70638619129503	39.0	35.0	41.0	31.0	41.0
31	36.707878481423016	39.0	35.0	41.0	31.0	41.0
32	36.53599995771551	39.0	35.0	41.0	30.0	41.0
33	36.398000119806056	39.0	35.0	40.0	30.0	41.0
34	36.33292164683994	39.0	35.0	40.0	30.0	41.0
35	36.153089234368835	38.0	35.0	40.0	30.0	41.0
36	36.11004802813328	38.0	35.0	40.0	30.0	41.0
37	36.019047400913344	38.0	35.0	40.0	30.0	41.0
38	35.92787499295258	38.0	35.0	40.0	29.0	41.0
39	35.84891135056661	38.0	35.0	40.0	29.0	41.0
40	35.757679039578285	38.0	35.0	40.0	29.0	41.0
41	35.69522749055646	38.0	35.0	40.0	29.0	41.0
42	35.59759700766759	38.0	35.0	40.0	29.0	41.0
43	35.52988984890342	38.0	34.0	40.0	29.0	41.0
44	35.46322042199921	37.0	34.0	40.0	28.0	41.0
45	35.393939927834474	37.0	34.0	40.0	28.0	41.0
46	35.2728662189209	37.0	34.0	40.0	28.0	41.0
47	35.170601179737275	37.0	34.0	40.0	28.0	41.0
48	35.08900973248013	37.0	34.0	40.0	27.0	41.0
49	35.02514253396854	37.0	34.0	40.0	27.0	41.0
50	34.94780772537633	36.0	34.0	40.0	27.0	41.0
51	34.23086538732593	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	40.0
10	79.0
11	59.0
12	67.0
13	58.0
14	74.0
15	104.0
16	170.0
17	316.0
18	475.0
19	881.0
20	1547.0
21	2691.0
22	3823.0
23	5733.0
24	7290.0
25	9360.0
26	11109.0
27	13337.0
28	15658.0
29	18695.0
30	23088.0
31	29212.0
32	37483.0
33	51665.0
34	84673.0
35	104762.0
36	107041.0
37	145485.0
38	206149.0
39	253901.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5071601736483	20.2037936234989	22.85397403732311	15.435072165529684
2	28.398087331566778	27.259048880870495	25.81882153126233	18.524042256300387
3	31.456753537802335	25.828335541523366	25.94593927947229	16.768971641202008
4	28.635761402717485	26.383495658792356	26.48374513728364	18.496997801206515
5	26.1156057957941	30.098276202289	23.74644105542087	20.039676946496023
6	25.21098198680724	36.50094082990359	22.6200879517393	15.667989231549868
7	80.35436164514856	6.813705107966397	8.421044285955912	4.410888960929131
8	81.78930343350059	6.64624090883464	6.480890934205333	5.0835647234594346
9	76.45899109206744	7.811266702373569	8.866705192535377	6.863037013023623
10	43.359308930484296	26.04425071883633	15.234749393922309	15.361690956757062
11	33.22812130010712	26.169342335231438	20.95363858036872	19.64889778429272
12	27.490204093138637	22.392368354287647	27.643573462254047	22.47385409031967
13	25.24005257371596	23.786963691717876	29.219375458081977	21.753608276484186
14	21.44493149912612	27.365464847493936	28.07126346056267	23.118340192817275
15	21.33093956136889	24.621641906748604	31.24726912668433	22.800149405198173
16	23.946235270902633	23.15930329255229	29.613678327789366	23.28078310875571
17	23.877875345323336	23.516871511529573	26.390719259175732	26.21453388397136
18	24.947056294751086	23.524447482663362	26.78167460675424	24.746821615831312
19	25.96294116817951	25.845689800981	25.172045018887072	23.019324011952417
20	27.475756892371876	24.474350933077744	24.55011064441563	23.499781530134747
21	25.77477518746124	26.679046625697694	24.80434614083554	22.741832046005523
22	24.37418954727406	24.350668799684275	24.88045822292383	26.394683430117833
23	24.11968977279134	25.819350087387942	24.933401928172746	25.127558211647965
24	23.594393076619497	25.351842194283137	26.698603202345378	24.355161526751985
25	24.524475672323394	25.703948666629078	25.317133675367877	24.454441985679654
26	22.763855217906073	24.96934374471444	26.11269873710323	26.15410230027626
27	24.37304434233523	24.459463268872977	25.87960548570784	25.28788690308395
28	22.707563990528275	26.993977983875517	26.430889524722335	23.86756850087388
29	24.528175565202684	25.503361616958898	24.736338586006653	25.232124231831765
30	24.188402069121047	25.60731098832948	25.490147713818573	24.714139228730904
31	25.325766758752888	25.609072842081527	23.055089643118905	26.010070756046684
32	26.454145994249313	26.488237864351355	22.713113829847213	24.344502311552123
33	24.65696707447708	25.864541636127868	23.066365507131984	26.412125782263068
34	24.168669307098156	25.093290156170717	24.133520324744882	26.604520211986245
35	24.108237723403057	25.88841475446806	24.268390229463833	25.73495729266505
36	24.580326436263178	27.38687137058127	23.405081890962396	24.627720302193158
37	23.69561157467441	27.881247533404746	24.47769845520663	23.945442436714213
38	23.64249168405029	28.2296541128714	24.13211084174325	23.995743361335062
39	24.20743008964312	26.55739062411907	24.455675283306082	24.779504002931724
40	24.356571009753623	24.71281783841687	26.86492219653831	24.065688955291197
41	21.983177820375484	26.005049472853358	25.66219273270564	26.349579974065513
42	23.18502635733213	26.178856345492473	25.275818204882448	25.360299092292948
43	23.237001043017422	24.44307802897897	26.275317838416868	26.044603089586737
44	23.903157946665164	24.25579297513672	26.09648968258443	25.744559395613685
45	23.754281304617468	24.206989626205107	26.31108346958336	25.72764559959407
46	23.795772960478097	25.35959435079213	25.910085555618203	24.934547133111575
47	23.197271240908833	25.78358445622146	26.442517759485824	24.576626543383888
48	23.028045188025033	25.690118114675535	26.07155945199301	25.210277245306422
49	23.18828578677341	25.562295624964765	26.4537055308113	24.795713057450527
50	22.86084526695608	24.65925748435474	27.226806957208094	25.253090291481083
51	22.730027625866832	24.83923084512601	25.683599255792977	26.747142273214187
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2125.0
1	2535.5
2	2946.0
3	4176.5
4	5407.0
5	3895.5
6	2384.0
7	2397.5
8	2411.0
9	2568.0
10	2725.0
11	2810.0
12	2895.0
13	2783.5
14	2672.0
15	2654.5
16	2637.0
17	2677.0
18	2717.0
19	2660.5
20	2604.0
21	2935.0
22	3266.0
23	3509.0
24	3752.0
25	5275.5
26	7169.5
27	7540.0
28	9588.0
29	11636.0
30	14301.5
31	16967.0
32	18089.5
33	19212.0
34	20688.5
35	22165.0
36	24511.5
37	26858.0
38	28421.0
39	29984.0
40	33700.0
41	37416.0
42	43194.0
43	48972.0
44	54563.0
45	60154.0
46	71481.0
47	82808.0
48	86022.5
49	89237.0
50	89201.0
51	89165.0
52	82553.5
53	75942.0
54	70676.5
55	65411.0
56	64337.5
57	63264.0
58	62221.5
59	61179.0
60	60076.5
61	58974.0
62	55501.5
63	52029.0
64	47779.0
65	43529.0
66	39494.0
67	35459.0
68	30462.0
69	25465.0
70	22220.0
71	18975.0
72	17696.5
73	16418.0
74	14412.5
75	10044.5
76	7682.0
77	6419.5
78	5157.0
79	3834.5
80	2512.0
81	2051.5
82	1591.0
83	1295.0
84	999.0
85	692.0
86	385.0
87	285.5
88	186.0
89	157.0
90	128.0
91	70.0
92	12.0
93	10.5
94	9.0
95	4.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1135168.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.983288061909885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.95386572574898	18.456067291781718
2	8.792152643429308	4.21729459063295
3	2.9890030543214716	2.15058363809161
4	1.4900054656158592	1.4294092118274127
5	0.8585232397240579	1.02951050830731
6	0.6495006580967547	0.9346296827720707
7	0.49319233033454074	0.8279861609836535
8	0.37428633968478736	0.7181293681838486
9	0.3186992265128622	0.6879109819109258
>10	4.999229900977647	31.748639359658686
>50	1.9413665428986322	31.001087530265576
>100	0.1320380726592896	4.654349857390871
>500	0.005547818178961731	1.0003956031265675
>1k	0.0025889818168488075	1.1440062150667976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2679	0.23600031008626038	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	2024	0.17829959970682752	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	2013	0.17733058014320346	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	1822	0.16050487681118566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1690	0.1488766420476969	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1565	0.13786505609742347	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.809268760218752E-5	0.0	0.0	0.0825428482832497	0.0
2	8.809268760218752E-5	0.0	0.0	0.4157093927947229	0.0
3	8.809268760218752E-5	0.0	0.0	0.5789451429215764	0.0
4	8.809268760218752E-5	0.0	0.0	0.7787393584033376	0.0
5	8.809268760218752E-5	0.0	0.0	1.46947412189209	0.0
6	8.809268760218752E-5	0.0	0.0	2.0362624739245643	0.0
7	1.7618537520437504E-4	0.0	0.0	2.58093956136889	0.0
8	1.7618537520437504E-4	0.0	0.0	3.5592969498787843	0.0
9	1.7618537520437504E-4	0.0	0.0	4.040283024186729	0.0
10	1.7618537520437504E-4	0.0	0.0	4.984548542594577	0.0
11	1.7618537520437504E-4	0.0	0.0	5.801608220104866	0.0
12	1.7618537520437504E-4	0.0	0.0	6.7008583751479955	0.0
13	1.7618537520437504E-4	0.0	0.0	7.013058860010148	0.0
14	1.7618537520437504E-4	0.0	0.0	7.146255003664656	0.0
15	1.7618537520437504E-4	0.0	0.0	7.3542418390934206	0.0
16	1.7618537520437504E-4	0.0	0.0	7.7329523030952245	0.0
17	1.7618537520437504E-4	0.0	0.0	8.171389609291312	0.0
18	1.7618537520437504E-4	0.0	0.0	8.734654253819699	0.0
19	2.6427806280656253E-4	0.0	0.0	9.053725968314822	0.0
20	2.6427806280656253E-4	0.0	0.0	9.335710661329424	0.0
21	3.523707504087501E-4	0.0	0.0	9.646237385127135	0.0
22	3.523707504087501E-4	0.0	0.0	9.956676016237244	0.0
23	3.523707504087501E-4	0.0	0.0	10.274162062355527	0.0
24	4.4046343801093757E-4	0.0	0.0	10.5428447595422	0.0
25	4.4046343801093757E-4	0.0	0.0	10.757262361165925	0.0
26	5.285561256131251E-4	0.0	0.0	10.964632547781473	0.0
27	6.166488132153126E-4	0.0	0.0	11.194994925861193	0.0
28	6.166488132153126E-4	0.0	0.0	11.41434571799064	0.0
29	6.166488132153126E-4	0.0	0.0	11.642505778880306	0.0
30	6.166488132153126E-4	0.0	0.0	11.924754749957716	0.0
31	6.166488132153126E-4	0.0	0.0	12.167450104301743	0.0
32	6.166488132153126E-4	0.0	0.0	12.413316795399448	0.0
33	7.047415008175001E-4	0.0	0.0	12.66429286237808	0.0
34	7.047415008175001E-4	0.0	0.0	12.896505186897446	0.0
35	7.047415008175001E-4	0.0	0.0	13.153559649320629	0.0
36	7.047415008175001E-4	0.0	0.0	13.38436249083836	0.0
37	7.047415008175001E-4	0.0	0.0	13.617720020296556	0.0
38	7.047415008175001E-4	0.0	0.0	13.861736764954614	0.0
39	7.047415008175001E-4	0.0	0.0	14.104432119298641	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTGAA	25	3.890774E-5	45.000004	18
ATTACGA	25	3.890774E-5	45.000004	27
TATGTCG	25	3.890774E-5	45.000004	21
TAATGCG	30	2.1654778E-6	45.000004	1
AAGTACG	20	7.0332515E-4	45.0	1
ATTACGC	20	7.0332515E-4	45.0	1
ATTTTCG	20	7.0332515E-4	45.0	14
TTACGTG	20	7.0332515E-4	45.0	1
GTATACG	45	3.8562575E-10	45.0	1
CCAGTCG	100	0.0	42.750004	28
CGTTCAT	75	0.0	42.0	17
TTGTGCG	135	0.0	40.0	1
TTAACGA	45	1.9283107E-8	40.0	18
ACCGGTA	40	3.4581717E-7	39.375	41
AATGCGG	115	0.0	39.130432	2
CTATGCG	35	6.2482195E-6	38.57143	1
TGTAACG	35	6.2482195E-6	38.57143	1
AGTACGG	95	0.0	37.894737	2
ACGTGCG	30	1.1399128E-4	37.500004	10
TCGTTCA	85	0.0	37.058826	16
>>END_MODULE