FastQCFastQC Report
Sat 18 Jun 2016
SRR3552642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences993885
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC29510.2969156391333001No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC29420.29601010177233783No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24790.24942523531394475No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22120.22256096027206368No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT20550.20676436408638824No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC16320.1642041081211609No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC13890.13975459937517923No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12710.12788199842034037No Hit
AAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC11020.11087801908671525No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT10730.10796017647917013No Hit
ACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC10570.10635033228190384No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC10060.10121895390311758No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA207.032824E-445.00000437
TCCGCGA207.032824E-445.00000417
TACGATA207.032824E-445.00000440
TGCGACC406.8139343E-945.00000414
GAACGAC207.032824E-445.00000411
ACGCTAG207.032824E-445.0000041
TCGTAAT207.032824E-445.00000433
TATGTCG207.032824E-445.00000422
CCGCAAT207.032824E-445.00000433
ATTTCGG207.032824E-445.0000042
CTATCGT253.8904232E-545.018
TAGCCGT453.8380676E-1045.044
GTATGCG351.2117016E-745.01
TATTACG502.1827873E-1145.01
GTATACG253.8904232E-545.01
TAGGACG502.1827873E-1145.01
TATCGTC253.8904232E-545.039
TGTAACG253.8904232E-545.01
TTCGAAC253.8904232E-545.026
TCTATAC253.8904232E-545.010