##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	993885
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57768554712064	31.0	31.0	34.0	30.0	34.0
2	31.72282004457256	31.0	31.0	34.0	30.0	34.0
3	31.757461879392483	33.0	31.0	34.0	30.0	34.0
4	35.42603017451717	37.0	35.0	37.0	33.0	37.0
5	35.259082288192296	37.0	35.0	37.0	33.0	37.0
6	35.25843231359765	37.0	35.0	37.0	32.0	37.0
7	35.63745403140202	37.0	35.0	37.0	35.0	37.0
8	35.42342926998597	37.0	35.0	37.0	33.0	37.0
9	37.070930741484176	39.0	37.0	39.0	33.0	39.0
10	36.66353350739774	39.0	35.0	39.0	32.0	39.0
11	36.634146807729266	39.0	35.0	39.0	32.0	39.0
12	36.54672421859672	39.0	35.0	39.0	32.0	39.0
13	36.37357843211237	39.0	35.0	39.0	32.0	39.0
14	37.46137531002078	39.0	36.0	41.0	32.0	41.0
15	37.61211005297393	39.0	36.0	41.0	33.0	41.0
16	37.64421839548841	39.0	36.0	41.0	33.0	41.0
17	37.59983499096978	39.0	36.0	41.0	33.0	41.0
18	37.57795117141319	39.0	36.0	41.0	33.0	41.0
19	37.518572068197024	39.0	36.0	41.0	32.0	41.0
20	37.42457729012914	39.0	35.0	41.0	32.0	41.0
21	37.3322517192633	39.0	35.0	41.0	32.0	41.0
22	37.346847975369386	39.0	35.0	41.0	32.0	41.0
23	37.26341377523556	39.0	35.0	41.0	32.0	41.0
24	37.25597528889157	39.0	35.0	41.0	32.0	41.0
25	37.015606433339876	39.0	35.0	41.0	31.0	41.0
26	37.00852814963502	39.0	35.0	41.0	31.0	41.0
27	36.77707280017306	39.0	35.0	40.0	31.0	41.0
28	36.70875302474632	39.0	35.0	40.0	31.0	41.0
29	36.60684787475412	39.0	35.0	40.0	31.0	41.0
30	36.4793985219618	38.0	35.0	40.0	30.0	41.0
31	36.43198659804706	38.0	35.0	40.0	30.0	41.0
32	36.36629489327236	38.0	35.0	40.0	30.0	41.0
33	36.23110017758594	38.0	35.0	40.0	30.0	41.0
34	36.22143205702873	38.0	35.0	40.0	30.0	41.0
35	36.14516266972537	38.0	35.0	40.0	30.0	41.0
36	36.11223733128078	38.0	35.0	40.0	30.0	41.0
37	36.09201567585787	38.0	35.0	40.0	30.0	41.0
38	35.997341744769265	38.0	35.0	40.0	30.0	41.0
39	35.902209008084434	38.0	35.0	40.0	29.0	41.0
40	35.80361108176499	38.0	35.0	40.0	29.0	41.0
41	35.67707632170724	38.0	35.0	40.0	29.0	41.0
42	35.63007289575756	38.0	34.0	40.0	29.0	41.0
43	35.52965483934258	38.0	34.0	40.0	28.0	41.0
44	35.47032000684184	38.0	34.0	40.0	28.0	41.0
45	35.45187018618854	38.0	34.0	40.0	28.0	41.0
46	35.34487289776986	37.0	34.0	40.0	28.0	41.0
47	35.251354029892795	37.0	34.0	40.0	28.0	41.0
48	35.19767276898233	37.0	34.0	40.0	28.0	41.0
49	35.124985285017885	37.0	34.0	40.0	27.0	41.0
50	35.03657867861976	37.0	34.0	40.0	27.0	41.0
51	34.2818696328046	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	33.0
10	70.0
11	64.0
12	61.0
13	46.0
14	55.0
15	83.0
16	137.0
17	287.0
18	505.0
19	808.0
20	1424.0
21	2121.0
22	3081.0
23	4478.0
24	6118.0
25	7698.0
26	9701.0
27	11744.0
28	14393.0
29	17274.0
30	21556.0
31	27114.0
32	34977.0
33	47062.0
34	74256.0
35	94906.0
36	94321.0
37	127517.0
38	184542.0
39	207358.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.395317365691206	20.72996372819793	23.64519033892251	15.229528567188359
2	29.81089361445238	25.6351589972683	26.042852040225984	18.511095348053345
3	30.814430240923247	24.87410515301066	26.79303943615207	17.518425169914025
4	27.664770068971762	27.553690819360387	25.916076809691262	18.86546230197659
5	23.785347399347007	29.967048501587207	25.093345809625863	21.154258289439927
6	23.04663014332644	34.915407718196775	25.386236838265997	16.65172530021079
7	78.36963028921858	7.357692288343219	8.804338530111632	5.468338892326577
8	79.13048290295154	6.196089084753266	8.268461642946619	6.404966369348567
9	73.18381905351222	8.706339264603049	11.128852935701817	6.980988746182909
10	39.44369821458217	27.117523657163556	17.84411677407346	15.594661354180817
11	29.349773867197914	26.22637427871434	23.67014292398014	20.75370893010761
12	25.728932421759055	21.10425250406234	31.651750454026374	21.51506462015223
13	23.79571077136691	24.205818580620495	30.775492134401865	21.22297851361073
14	18.861135845696435	28.37159228683399	28.829592960956248	23.93767890651333
15	18.32857926218828	25.001383459857024	34.66356771658693	22.006469561367766
16	20.599163887170043	24.11375561558933	31.63887170044824	23.648208796792385
17	20.000905537360964	24.002776981240284	27.858253218430708	28.138064262968047
18	23.16424938498921	22.845802079717473	29.38438551743914	24.60556301785418
19	24.310257222918143	26.622295335979512	25.38019992252625	23.687247518576093
20	24.1744266187738	24.932562620423894	25.964875211920894	24.92813554888141
21	23.490947141771937	27.62190796721955	25.828440916202577	23.058703974805937
22	20.61123771864954	24.74964407350951	25.392173138743416	29.246945069097528
23	19.733671400614757	27.44412079868395	25.67228602906775	27.14992177163354
24	21.22871358356349	25.13661037242739	28.55008376220589	25.084592281803225
25	21.163816739361195	26.496928719117403	26.35023166664151	25.98902287487989
26	19.721295723348277	25.732655186465237	27.16702636622949	27.379022723956997
27	21.510939394396736	25.515426835096616	26.908445142043597	26.065188628463055
28	20.47560834502986	25.242256397872993	28.762784426769695	25.519350830327454
29	21.148120758437848	23.758583739567456	26.99708718815557	28.096208313839128
30	22.422111210049454	24.039099090941104	27.445931873405875	26.092857825603566
31	22.0224673880781	26.234725345487657	24.246366531339138	27.496440735095106
32	22.315257801455903	25.367723629997435	25.188326617264572	27.128691951282093
33	21.351363588342714	23.921781695065324	26.39711837888689	28.329736337705064
34	20.51997967571701	23.05276767432852	26.64533623105289	29.781916418901584
35	19.73719293479628	21.806848880906745	28.477640773328904	29.97831741096807
36	21.29220181409318	24.340240571092227	27.30154897196356	27.066008642851035
37	20.285546114490106	24.981763483702842	29.384486132701472	25.34820426910558
38	21.093084209943807	25.673795258002684	25.731347188054954	27.501773343998554
39	21.952036704447696	23.59256855672437	27.500062884538956	26.95533185428898
40	22.664694607525014	21.857156512071317	29.265257046841437	26.212891833562235
41	19.678836082645375	23.67849399075346	27.141067628548576	29.501602298052592
42	21.46133607006847	24.066164596507644	26.944062944908115	27.528436388515775
43	21.60662450887175	24.023403110017757	28.398657792400527	25.97131458870996
44	21.68429949138985	23.073796264155312	27.854429838462195	27.387474405992645
45	21.751611101888045	22.04339536264256	28.204772181892274	28.000221353577125
46	23.148251558278876	23.29444553444312	27.309296347162903	26.248006560115105
47	20.44602745790509	22.92951397797532	29.92921716295145	26.69524140116814
48	20.46222651514008	22.72174346126564	27.872238739894456	28.943791283699827
49	21.974675138471756	22.16654844373343	30.03908902941487	25.81968738837994
50	21.32027347228301	21.128500782283666	30.46650266378907	27.084723081644253
51	20.77342952152412	21.75704432605382	27.557413584066566	29.912112568355493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2990.0
1	3148.5
2	3307.0
3	3897.5
4	4488.0
5	3306.5
6	2125.0
7	2135.0
8	2145.0
9	2303.5
10	2462.0
11	2543.0
12	2624.0
13	2668.0
14	2712.0
15	2526.0
16	2340.0
17	2401.0
18	2462.0
19	2512.5
20	2563.0
21	2933.5
22	3304.0
23	3351.5
24	3399.0
25	4011.5
26	6042.0
27	7460.0
28	8382.5
29	9305.0
30	10748.5
31	12192.0
32	12606.5
33	13021.0
34	15125.5
35	17230.0
36	20589.0
37	23948.0
38	24377.0
39	24806.0
40	27521.5
41	30237.0
42	36707.0
43	43177.0
44	53547.5
45	63918.0
46	74102.5
47	84287.0
48	93862.0
49	103437.0
50	100265.0
51	97093.0
52	86253.0
53	75413.0
54	67404.0
55	59395.0
56	54476.5
57	49558.0
58	47890.0
59	46222.0
60	45092.0
61	43962.0
62	41023.0
63	38084.0
64	33276.5
65	28469.0
66	24480.5
67	20492.0
68	19051.0
69	17610.0
70	15087.0
71	12564.0
72	11766.5
73	10969.0
74	9433.5
75	6426.5
76	4955.0
77	4176.0
78	3397.0
79	2562.5
80	1728.0
81	1265.5
82	803.0
83	657.0
84	511.0
85	302.5
86	94.0
87	85.5
88	77.0
89	44.0
90	11.0
91	12.0
92	13.0
93	7.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	993885.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.08797887938106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6151971786236	20.482628641347905
2	10.09714554676499	5.470225306256132
3	4.07524928303098	3.3117079952086805
4	1.992840213375973	2.1592805443963843
5	1.1407679294467474	1.545054878956438
6	0.7516874757620303	1.2217016680402284
7	0.5492142450972142	1.0413972710013986
8	0.41761772929471175	0.9049936184632185
9	0.30750717199808114	0.7496773002307999
>10	3.2350105201004857	21.149465683483967
>50	1.5648175476153345	29.907989841407655
>100	0.24359067816169858	9.069679918218036
>500	0.004864329978651426	0.8405584984888816
>1k	0.004490150749524393	2.1456388345003177
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2951	0.2969156391333001	No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	2942	0.29601010177233783	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2479	0.24942523531394475	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2212	0.22256096027206368	No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT	2055	0.20676436408638824	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1632	0.1642041081211609	No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1389	0.13975459937517923	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1271	0.12788199842034037	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTC	1102	0.11087801908671525	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1073	0.10796017647917013	No Hit
ACTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC	1057	0.10635033228190384	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCC	1006	0.10121895390311758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0123052465828543E-4	0.0	0.0	0.2172283513686191	0.0
2	2.0123052465828543E-4	0.0	0.0	1.1071703466698863	0.0
3	2.0123052465828543E-4	0.0	0.0	1.5159701575131932	0.0
4	3.018457869874281E-4	0.0	0.0	2.0456088984138003	0.0
5	3.018457869874281E-4	0.0	0.0	3.550008300759142	0.0
6	3.018457869874281E-4	0.0	0.0	4.911030954285455	0.0
7	3.018457869874281E-4	0.0	0.0	6.157654054543534	0.0
8	3.018457869874281E-4	0.0	0.0	8.122368282044704	0.0
9	3.018457869874281E-4	0.0	0.0	9.094814792455868	0.0
10	3.018457869874281E-4	0.0	0.0	10.787163504832048	0.0
11	4.0246104931657085E-4	0.0	0.0	12.414112296694286	0.0
12	4.0246104931657085E-4	0.0	0.0	14.04267093275379	0.0
13	4.0246104931657085E-4	0.0	0.0	14.676848931214376	0.0
14	4.0246104931657085E-4	0.0	0.0	14.96390427463942	0.0
15	5.030763116457136E-4	0.0	0.0	15.310825699150303	0.0
16	6.036915739748562E-4	0.0	0.0	16.010303002862504	0.0
17	6.036915739748562E-4	0.0	0.0	16.898534538704176	0.0
18	6.036915739748562E-4	0.0	0.0	18.07623618426679	0.0
19	6.036915739748562E-4	0.0	0.0	18.59269432580228	0.0
20	7.04306836303999E-4	0.0	0.0	19.107039546828858	0.0
21	7.04306836303999E-4	0.0	0.0	19.622994612052704	0.0
22	7.04306836303999E-4	0.0	0.0	20.15263335295331	0.0
23	7.04306836303999E-4	0.0	0.0	20.68086348018131	0.0
24	7.04306836303999E-4	0.0	0.0	21.106164194046595	0.0
25	7.04306836303999E-4	0.0	0.0	21.45550038485338	0.0
26	7.04306836303999E-4	0.0	0.0	21.77797230061828	0.0
27	7.04306836303999E-4	0.0	0.0	22.136464480297015	0.0
28	8.049220986331417E-4	0.0	0.0	22.50572249304497	0.0
29	8.049220986331417E-4	0.0	0.0	22.893393098799155	0.0
30	0.0011067678856205698	0.0	0.0	23.36719036910709	0.0
31	0.0011067678856205698	0.0	0.0	23.769148342112015	0.0
32	0.0011067678856205698	0.0	0.0	24.142330350090806	0.0
33	0.0011067678856205698	0.0	0.0	24.533019413714868	0.0
34	0.0011067678856205698	0.0	0.0	24.92924231676703	0.0
35	0.0011067678856205698	0.0	0.0	25.36239102109399	0.0
36	0.0011067678856205698	0.0	0.0	25.71776412764052	0.0
37	0.0011067678856205698	0.0	0.0	26.068207086332926	0.0
38	0.0011067678856205698	0.0	0.0	26.43273618175141	0.0
39	0.0011067678856205698	0.0	0.0	26.824129552211776	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	20	7.032824E-4	45.000004	37
TCCGCGA	20	7.032824E-4	45.000004	17
TACGATA	20	7.032824E-4	45.000004	40
TGCGACC	40	6.8139343E-9	45.000004	14
GAACGAC	20	7.032824E-4	45.000004	11
ACGCTAG	20	7.032824E-4	45.000004	1
TCGTAAT	20	7.032824E-4	45.000004	33
TATGTCG	20	7.032824E-4	45.000004	22
CCGCAAT	20	7.032824E-4	45.000004	33
ATTTCGG	20	7.032824E-4	45.000004	2
CTATCGT	25	3.8904232E-5	45.0	18
TAGCCGT	45	3.8380676E-10	45.0	44
GTATGCG	35	1.2117016E-7	45.0	1
TATTACG	50	2.1827873E-11	45.0	1
GTATACG	25	3.8904232E-5	45.0	1
TAGGACG	50	2.1827873E-11	45.0	1
TATCGTC	25	3.8904232E-5	45.0	39
TGTAACG	25	3.8904232E-5	45.0	1
TTCGAAC	25	3.8904232E-5	45.0	26
TCTATAC	25	3.8904232E-5	45.0	10
>>END_MODULE