##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552641_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 680827 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80195850047075 31.0 31.0 34.0 30.0 34.0 2 31.95457876964339 33.0 31.0 34.0 30.0 34.0 3 31.980726381298037 33.0 31.0 34.0 30.0 34.0 4 35.62794953784148 37.0 35.0 37.0 33.0 37.0 5 35.4376177795534 37.0 35.0 37.0 33.0 37.0 6 35.44152185503806 37.0 35.0 37.0 33.0 37.0 7 35.75133918014415 37.0 35.0 37.0 35.0 37.0 8 35.47456255407027 37.0 35.0 37.0 33.0 37.0 9 37.18196546259182 39.0 37.0 39.0 34.0 39.0 10 36.861590389335326 39.0 37.0 39.0 32.0 39.0 11 36.78786387731392 39.0 37.0 39.0 32.0 39.0 12 36.63666100198729 39.0 35.0 39.0 32.0 39.0 13 36.40738983618452 39.0 35.0 39.0 32.0 39.0 14 37.52041414338738 40.0 36.0 41.0 32.0 41.0 15 37.672142849798846 40.0 36.0 41.0 33.0 41.0 16 37.742423552532436 40.0 36.0 41.0 33.0 41.0 17 37.66306565397671 40.0 36.0 41.0 33.0 41.0 18 37.64458225070393 39.0 36.0 41.0 33.0 41.0 19 37.57421048225173 39.0 36.0 41.0 33.0 41.0 20 37.42474226198432 39.0 35.0 41.0 32.0 41.0 21 37.366474890096896 39.0 35.0 41.0 32.0 41.0 22 37.41522295678638 39.0 35.0 41.0 32.0 41.0 23 37.25555684483724 39.0 35.0 41.0 32.0 41.0 24 37.28372112151839 39.0 35.0 41.0 32.0 41.0 25 37.15538161676902 39.0 35.0 41.0 32.0 41.0 26 37.13714203461379 39.0 35.0 41.0 32.0 41.0 27 36.8879333516444 39.0 35.0 41.0 31.0 41.0 28 36.88274701208971 39.0 35.0 41.0 31.0 41.0 29 36.873048513058386 39.0 35.0 41.0 31.0 41.0 30 36.73150594791335 39.0 35.0 41.0 31.0 41.0 31 36.71948380425571 39.0 35.0 41.0 31.0 41.0 32 36.5355604287139 39.0 35.0 41.0 31.0 41.0 33 36.363337529210796 39.0 35.0 40.0 30.0 41.0 34 36.211929021616356 38.0 35.0 40.0 30.0 41.0 35 36.08124971541963 38.0 35.0 40.0 30.0 41.0 36 36.01520503740304 38.0 35.0 40.0 30.0 41.0 37 35.92466514988389 38.0 35.0 40.0 30.0 41.0 38 35.83931600832517 38.0 35.0 40.0 29.0 41.0 39 35.78062562148681 38.0 35.0 40.0 29.0 41.0 40 35.66871613493589 38.0 35.0 40.0 29.0 41.0 41 35.59213867840141 38.0 35.0 40.0 29.0 41.0 42 35.52635397832342 38.0 35.0 40.0 29.0 41.0 43 35.43855487517387 38.0 35.0 40.0 28.0 41.0 44 35.36164840701088 38.0 34.0 40.0 28.0 41.0 45 35.32280447162054 37.0 34.0 40.0 28.0 41.0 46 35.18274980281334 37.0 34.0 40.0 27.0 41.0 47 35.09000965002857 37.0 34.0 40.0 27.0 41.0 48 35.04398914849147 37.0 34.0 40.0 27.0 41.0 49 34.98057362589909 37.0 34.0 40.0 27.0 41.0 50 34.87571732613424 36.0 34.0 40.0 27.0 41.0 51 34.201018761006836 36.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 4.0 9 17.0 10 38.0 11 38.0 12 39.0 13 33.0 14 50.0 15 87.0 16 94.0 17 191.0 18 322.0 19 579.0 20 1173.0 21 1893.0 22 2838.0 23 3868.0 24 4857.0 25 5796.0 26 6743.0 27 7866.0 28 9001.0 29 10610.0 30 13271.0 31 17058.0 32 22131.0 33 30065.0 34 52384.0 35 66864.0 36 60730.0 37 83909.0 38 123453.0 39 154740.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.15350742552807 19.31386387437631 23.29387641794465 13.238752282150973 2 29.90113494323815 27.86552237205634 25.362390151976932 16.870952532728577 3 30.201798694822617 27.857297081343717 25.912309588192006 16.028594635641653 4 27.21734008786373 26.62320971406833 27.616266687425732 18.543183510642205 5 26.535522239864168 30.373501638448534 23.870234288593135 19.220741833094163 6 24.417509881364868 36.676571287566446 23.617747239754006 15.288171591314681 7 78.42594374194914 7.20785162750596 9.596564178565187 4.769640451979725 8 79.69425419379667 8.100589430207673 6.9844468565435855 5.220709519452078 9 73.9873712411523 8.92517482414769 9.015799902177793 8.071654032522211 10 43.2904394214683 26.514959013082617 15.767735415898606 14.426866149550474 11 33.64320157690573 26.075493480722713 21.86708223968791 18.414222702683645 12 28.786754931869623 21.449061215257327 28.21142522256021 21.55275863031284 13 25.854291912629783 24.60742596871158 28.437326956774626 21.100955161884002 14 20.1487308817071 29.606933920070738 27.75198398418394 22.49235121403822 15 19.40096382781529 24.752837358095377 33.0644936231965 22.78170519089284 16 21.35593917397518 23.4003645566348 31.044597232483433 24.199099036906585 17 21.714473720930574 24.103480032372392 27.92662453163579 26.25542171506124 18 23.15830600137774 24.14181576230085 28.34963948257046 24.35023875375095 19 25.319354255926985 26.73043225371497 25.504129536578308 22.44608395377974 20 27.117461557781937 25.213600518193314 25.062901441922836 22.606036482101917 21 23.702937750706127 28.636349615981736 25.870742494055026 21.789970139257107 22 21.757068976406636 25.761463631730237 25.921269279861107 26.56019811200202 23 23.138036534978784 27.566180542193536 24.69907920808076 24.59670371474692 24 22.12544449617891 25.824181473413947 27.833502490353645 24.216871540053493 25 21.782332369309678 27.635214232103017 26.010719316360838 24.571734082226467 26 20.296932994725534 26.779490237608083 27.540329628525306 25.383247139141073 27 22.553453373617675 27.34762281754396 26.749673558774845 23.349250250063523 28 20.455563601326034 27.714676415594564 28.363739980934948 23.46602000214445 29 22.873799070835908 24.92351214038221 28.03884099778652 24.163847790995362 30 23.064449559139106 28.0758548059933 26.091503421574057 22.76819221329354 31 25.529246049290055 26.774643191295294 23.12643299986634 24.569677759548313 32 27.03917441582076 27.80706405592023 22.99115634368202 22.162605184576993 33 26.621006511198882 27.177094915448418 22.480747678925777 23.721150894426927 34 24.074985275260822 26.206657491550718 26.003081546413405 23.715275686775055 35 23.705287833766874 25.605917509146963 25.779529895259735 24.909264761826424 36 25.519111316090576 28.487266221815528 24.736533656861432 21.25708880523246 37 25.623543132102576 27.915020996523342 25.40190092343576 21.059534947938317 38 24.990930148187427 28.172648852057865 23.318846050465098 23.517574949289614 39 25.787461425589758 25.668929111213274 26.195935237586056 22.347674225610913 40 23.682815164498468 25.189659047011943 27.59717226255716 23.530353525932433 41 21.783654291031347 24.341572822464443 27.435750932322012 26.439021954182195 42 23.117913948771125 24.177360768594667 27.168282103970615 25.5364431786636 43 24.759740727086324 24.430141577816393 26.147024133884234 24.663093561213053 44 24.007714147646904 25.126500564754334 26.112507288929493 24.753277998669265 45 23.996551253108354 24.898101867287874 26.144674050823486 24.960672828780293 46 24.431316619346767 26.369400743507526 25.866776728890013 23.332505908255698 47 22.82165660292556 25.74706937298315 28.405894595837122 23.025379428254166 48 23.26699734293734 24.495503262943448 27.22644665972413 25.011052734395083 49 23.38450149597475 23.784603137067126 28.426457822618666 24.40443754433946 50 22.366181129714303 24.276651777911276 28.182196064492153 25.174971027882265 51 22.52143349191498 23.92972076606833 26.382473080532943 27.166372661483756 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2371.0 1 2626.0 2 2881.0 3 3503.0 4 4125.0 5 2978.5 6 1832.0 7 1862.0 8 1892.0 9 1986.0 10 2080.0 11 2124.0 12 2168.0 13 2300.5 14 2433.0 15 2378.5 16 2324.0 17 2209.5 18 2095.0 19 2059.5 20 2024.0 21 2204.5 22 2385.0 23 2749.0 24 3113.0 25 3243.5 26 4120.5 27 4867.0 28 6202.5 29 7538.0 30 8723.5 31 9909.0 32 10557.5 33 11206.0 34 12595.5 35 13985.0 36 15462.0 37 16939.0 38 17519.0 39 18099.0 40 19273.0 41 20447.0 42 26860.0 43 33273.0 44 36255.0 45 39237.0 46 47489.0 47 55741.0 48 56138.0 49 56535.0 50 57024.5 51 57514.0 52 52460.0 53 47406.0 54 44337.5 55 41269.0 56 38240.0 57 35211.0 58 34754.5 59 34298.0 60 32749.0 61 31200.0 62 29557.0 63 27914.0 64 25778.5 65 23643.0 66 19789.5 67 15936.0 68 13694.0 69 11452.0 70 10614.5 71 9777.0 72 8993.0 73 8209.0 74 6694.0 75 4489.5 76 3800.0 77 3127.5 78 2455.0 79 1784.0 80 1113.0 81 923.0 82 733.0 83 634.5 84 536.0 85 359.5 86 183.0 87 150.5 88 118.0 89 61.0 90 4.0 91 2.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 680827.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.827512540402388 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26981849064313 19.95687206061702 2 9.041865501692769 4.670577892672034 3 3.279265853074077 2.5408583983075212 4 1.5674251436682127 1.6193077021693112 5 0.9542705143808075 1.2323216838553273 6 0.6715324703716568 1.0406407979886816 7 0.4645449237415188 0.8398627890460043 8 0.3543776355799344 0.7322154261583123 9 0.29221193446635485 0.6792396661696519 >10 4.27477164937058 28.716739008587627 >50 1.6647548304843127 29.07671762638227 >100 0.15487420496438337 5.541718850739221 >500 0.004571932249871095 0.7631025824562997 >1k 0.005714915312338869 2.5898255148507228 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC 2849 0.4184616650044725 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG 2752 0.4042142864486867 No Hit GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC 2182 0.3204925774095328 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1928 0.2831850088201555 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.27613475963791095 No Hit GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC 1397 0.2051916272415753 No Hit CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC 1231 0.18080951548631297 No Hit ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC 1176 0.17273110496499108 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1091 0.1602462887047664 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 1060 0.15569300277456682 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT 816 0.1198542360981571 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCC 712 0.10457869620329394 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.10501933677718422 0.0 2 1.4688019129676115E-4 0.0 0.0 0.5991243002994887 0.0 3 1.4688019129676115E-4 0.0 0.0 0.7991751208456774 0.0 4 1.4688019129676115E-4 0.0 0.0 1.0839758117700973 0.0 5 1.4688019129676115E-4 0.0 0.0 2.129322133229146 0.0 6 1.4688019129676115E-4 0.0 0.0 2.7894017129167907 0.0 7 1.4688019129676115E-4 0.0 0.0 3.4845856583243613 0.0 8 1.4688019129676115E-4 0.0 0.0 4.549760805608473 0.0 9 1.4688019129676115E-4 0.0 0.0 5.126118676256964 0.0 10 1.4688019129676115E-4 0.0 0.0 6.6028521195546 0.0 11 1.4688019129676115E-4 0.0 0.0 7.849277422898916 0.0 12 1.4688019129676115E-4 0.0 0.0 9.312644768788546 0.0 13 1.4688019129676115E-4 0.0 0.0 9.75739798803514 0.0 14 1.4688019129676115E-4 0.0 0.0 9.952601762268536 0.0 15 1.4688019129676115E-4 0.0 0.0 10.305114221380762 0.0 16 1.4688019129676115E-4 0.0 0.0 10.8475427678397 0.0 17 1.4688019129676115E-4 0.0 0.0 11.49102488591081 0.0 18 1.4688019129676115E-4 0.0 0.0 12.311497634494518 0.0 19 1.4688019129676115E-4 0.0 0.0 12.83115975130246 0.0 20 2.937603825935223E-4 0.0 0.0 13.251677738985087 0.0 21 2.937603825935223E-4 0.0 0.0 13.6592702698336 0.0 22 2.937603825935223E-4 0.0 0.0 14.078172575411962 0.0 23 2.937603825935223E-4 0.0 0.0 14.476217893826185 0.0 24 2.937603825935223E-4 0.0 0.0 14.802438798696292 0.0 25 2.937603825935223E-4 0.0 0.0 15.065794981691385 0.0 26 4.4064057389028345E-4 0.0 0.0 15.331795008129818 0.0 27 4.4064057389028345E-4 0.0 0.0 15.646265497696184 0.0 28 4.4064057389028345E-4 0.0 0.0 15.916231289299631 0.0 29 4.4064057389028345E-4 0.0 0.0 16.19618493391126 0.0 30 4.4064057389028345E-4 0.0 0.0 16.53312809274603 0.0 31 4.4064057389028345E-4 0.0 0.0 16.81234733640117 0.0 32 5.875207651870446E-4 0.0 0.0 17.11565493142898 0.0 33 5.875207651870446E-4 0.0 0.0 17.388558326858366 0.0 34 5.875207651870446E-4 0.0 0.0 17.66909949223518 0.0 35 5.875207651870446E-4 0.0 0.0 17.96418179655037 0.0 36 5.875207651870446E-4 0.0 0.0 18.249423128048683 0.0 37 5.875207651870446E-4 0.0 0.0 18.54656175504203 0.0 38 5.875207651870446E-4 0.0 0.0 18.820787072193085 0.0 39 5.875207651870446E-4 0.0 0.0 19.109553528282515 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTCT 35 1.2109558E-7 45.000004 17 CATAGCG 65 0.0 45.000004 1 GTATGCG 25 3.889123E-5 45.000004 1 TCATCGG 25 3.889123E-5 45.000004 2 CAATGCG 25 3.889123E-5 45.000004 1 GCGTATA 25 3.889123E-5 45.000004 19 TACAACG 50 2.1827873E-11 45.000004 1 AACCGTA 20 7.0312584E-4 45.0 24 ATTAGCG 40 6.8084773E-9 45.0 1 ACGTTAT 20 7.0312584E-4 45.0 18 ACCGCGT 20 7.0312584E-4 45.0 39 CGTTAGT 20 7.0312584E-4 45.0 34 CGTTACA 20 7.0312584E-4 45.0 28 TACTATG 20 7.0312584E-4 45.0 22 AGTTACG 20 7.0312584E-4 45.0 1 TTACGAG 45 3.8380676E-10 45.0 1 ACGTATG 20 7.0312584E-4 45.0 1 TAGTGCG 20 7.0312584E-4 45.0 2 ACGAGTC 20 7.0312584E-4 45.0 45 GCGTACG 20 7.0312584E-4 45.0 37 >>END_MODULE