FastQCFastQC Report
Sat 18 Jun 2016
SRR3552640_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552640_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008612
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC42140.20979661577248368TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC33840.1684745485937553TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30750.15309079105372267No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT24840.12366748779754377TruSeq Adapter, Index 16 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG551.8189894E-1245.0000041
CGAATCG253.8918522E-545.037
GTACGTA207.0345483E-445.030
TCGATAG556.184564E-1140.9090921
CGCATGG3500.039.2142872
TATAGCG356.2506224E-638.5714261
TACGAAT2500.037.812
GTCGACA301.14022565E-437.50000417
TCGTAAG601.5643309E-1037.5000041
TATACGC301.14022565E-437.50000439
TCGAAAC301.14022565E-437.50000439
CGGGATA2700.036.6666646
CGTTCAT2300.036.19565217
CATAGCG250.00210748336.03
GAACGTA250.00210748336.09
CGTACGA250.00210748336.02
TCGAACA250.00210748336.033
AGTACGG3200.035.8593752
TACGGGA5150.035.825244
CGAATAT2650.035.66037814
Produced by FastQC (version 0.11.5)