##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552640_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2008612
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74643833652293	31.0	31.0	34.0	30.0	34.0
2	31.882654788480803	33.0	31.0	34.0	30.0	34.0
3	31.943219496846577	33.0	31.0	34.0	30.0	34.0
4	35.57613814912985	37.0	35.0	37.0	33.0	37.0
5	35.38639468448859	37.0	35.0	37.0	33.0	37.0
6	35.38318600107935	37.0	35.0	37.0	33.0	37.0
7	35.772093863822384	37.0	35.0	37.0	35.0	37.0
8	35.59665281298728	37.0	35.0	37.0	35.0	37.0
9	37.300042019065906	39.0	37.0	39.0	34.0	39.0
10	36.82646324924873	39.0	37.0	39.0	32.0	39.0
11	36.74318136105928	39.0	37.0	39.0	32.0	39.0
12	36.646473783886584	39.0	35.0	39.0	32.0	39.0
13	36.48361903642913	39.0	35.0	39.0	32.0	39.0
14	37.59815584094888	40.0	36.0	41.0	32.0	41.0
15	37.730002608766654	40.0	36.0	41.0	33.0	41.0
16	37.768887669694294	40.0	36.0	41.0	33.0	41.0
17	37.74902171250595	40.0	36.0	41.0	33.0	41.0
18	37.73075686095672	40.0	36.0	41.0	33.0	41.0
19	37.70572913036465	40.0	36.0	41.0	33.0	41.0
20	37.65079915882211	39.0	36.0	41.0	33.0	41.0
21	37.560891302053356	39.0	36.0	41.0	32.0	41.0
22	37.58522601677178	39.0	36.0	41.0	33.0	41.0
23	37.49975057402823	39.0	36.0	41.0	32.0	41.0
24	37.49716620233276	39.0	36.0	41.0	32.0	41.0
25	37.33235736916836	39.0	35.0	41.0	32.0	41.0
26	37.323930654601284	39.0	35.0	41.0	32.0	41.0
27	37.102761011086265	39.0	35.0	41.0	31.0	41.0
28	37.11088204192746	39.0	35.0	41.0	31.0	41.0
29	37.08948816396596	39.0	35.0	41.0	31.0	41.0
30	36.98024954545726	39.0	35.0	41.0	31.0	41.0
31	36.97564337960741	39.0	35.0	41.0	31.0	41.0
32	36.90059752704853	39.0	35.0	41.0	31.0	41.0
33	36.80542235135506	39.0	35.0	41.0	31.0	41.0
34	36.729165712442224	39.0	35.0	41.0	31.0	41.0
35	36.621888647483935	39.0	35.0	41.0	30.0	41.0
36	36.58165489402632	39.0	35.0	41.0	30.0	41.0
37	36.53385322800023	39.0	35.0	40.0	30.0	41.0
38	36.436219638237745	38.0	35.0	40.0	30.0	41.0
39	36.40671418870344	38.0	35.0	40.0	30.0	41.0
40	36.322598889183176	38.0	35.0	40.0	30.0	41.0
41	36.2414045121706	38.0	35.0	40.0	30.0	41.0
42	36.20004311434961	38.0	35.0	40.0	30.0	41.0
43	36.12864605010824	38.0	35.0	40.0	30.0	41.0
44	36.04819447459241	38.0	35.0	40.0	30.0	41.0
45	35.98058609626946	38.0	35.0	40.0	30.0	41.0
46	35.89091024050439	38.0	35.0	40.0	30.0	41.0
47	35.81294794614391	38.0	35.0	40.0	30.0	41.0
48	35.739549001997396	37.0	34.0	40.0	30.0	41.0
49	35.69377610011291	37.0	34.0	40.0	29.0	41.0
50	35.61406633038138	37.0	34.0	40.0	29.0	41.0
51	34.918815082255804	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	24.0
9	71.0
10	102.0
11	99.0
12	90.0
13	90.0
14	100.0
15	131.0
16	251.0
17	379.0
18	711.0
19	1163.0
20	1834.0
21	2817.0
22	4259.0
23	6264.0
24	8919.0
25	11981.0
26	15680.0
27	19983.0
28	24537.0
29	30467.0
30	38643.0
31	49929.0
32	65147.0
33	89646.0
34	144824.0
35	173121.0
36	186319.0
37	259949.0
38	389482.0
39	481375.0
40	224.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42117243150992	18.474498808132182	22.774433290252176	14.329895470105726
2	29.955461781568566	25.10499787913246	25.695256226687878	19.244284112611094
3	32.95474686002075	23.733852033145276	25.81145587101939	17.499945235814582
4	29.49872847518585	26.63944056891027	24.197306398647424	19.664524557256456
5	25.21223611130472	30.310632416813206	23.986563856035907	20.490567615846167
6	25.339886448950814	35.430834825242506	23.413332191583045	15.815946534223633
7	82.24535151637052	6.047160925056706	7.0011032494080485	4.706384309164736
8	83.26759971562451	5.020083520361324	6.51967627396431	5.192640490049846
9	77.4483075875281	6.796135839076935	9.35417093993265	6.401385633462311
10	40.80957397446595	25.901368706350453	16.09399923927568	17.195058079907916
11	30.54094070930573	26.80925932932791	22.201898624522805	20.44790133684355
12	27.218447365643538	23.23883358259335	28.365159622664805	21.177559429098302
13	25.329033183113513	23.554574004337322	30.034521351062327	21.081871461486838
14	22.31187506596595	26.689873405117563	28.216499752067598	22.78175177684889
15	21.664164109345162	25.282832124870307	31.32297327706894	21.73003048871559
16	23.897596947543875	24.18371492353924	30.03750848844874	21.881179640468144
17	24.33137908167431	23.540833172359818	27.120618616238477	25.00716912972739
18	25.376827381296142	23.750430645639874	27.754090884650694	23.11865108841329
19	25.34043409080499	26.347049604403438	25.57666687244724	22.735849432344327
20	26.53827618275705	25.451356459087172	25.7357817238969	22.27458563425888
21	26.665279307302754	25.96554237453525	25.768690020770563	21.600488297391433
22	23.679983988943608	24.516233100270238	25.45986980063845	26.343913110147703
23	23.419704751340728	26.643025133773968	25.58976049132436	24.34750962356095
24	23.326057994276646	24.984815384952395	27.102297506935138	24.586829113835822
25	24.21269015618746	25.643031108048742	25.681067324102415	24.463211411661387
26	22.734754148635975	25.83032462217691	26.205459292287408	25.22946193689971
27	23.881565976903456	25.09399525642583	26.409729703895028	24.614709062775688
28	23.059306625669866	26.335847839204384	26.96404283156727	23.640802703558478
29	23.325958423030432	25.822209565610482	25.611118523637217	25.240713487721873
30	24.206815452660845	24.79045231234305	25.930294153375566	25.07243808162054
31	24.895300834606186	25.83077269278487	23.810073822121943	25.463852650487002
32	24.454399356371464	26.536981756556266	24.191132981382168	24.8174859056901
33	23.644636196537707	25.046997628212914	25.26784665231513	26.040519522934247
34	23.00593643769927	25.381357872998866	25.81205329849667	25.800652390805194
35	23.030082464906116	24.994623152704452	26.30174468737616	25.67354969501327
36	23.928961890101224	25.921930168693606	25.737325078213214	24.411782862991956
37	23.41706611331606	26.116990240026443	26.166278006902278	24.299665639755215
38	23.308682811812336	26.866114510915995	26.142430693434072	23.682771983837593
39	23.518081142599964	24.10495406778412	26.25400027481664	26.122964514799275
40	23.4267245241988	24.139306147727886	28.302330166303896	24.13163916176942
41	21.51117288953765	24.165543171105224	28.469311146204447	25.853972793152685
42	22.760742243897777	24.543963692340782	27.86008447624529	24.835209587516154
43	23.08365179536914	23.565676198290163	28.45701409729704	24.89365790904366
44	23.99522655445651	24.125415958881057	27.39468847144197	24.48466901522046
45	23.507974661109262	23.682323913229634	26.135410920576003	26.674290505085104
46	23.41119140978945	24.50363733762419	26.415156336813677	25.670014915772683
47	22.403580183728863	24.932241766951506	27.985494460851573	24.678683588468058
48	22.601328678709475	24.401427453385722	27.676425312603925	25.32081855530087
49	23.169581780851654	24.12217989337911	28.072868229404186	24.635370096365055
50	21.77100405653257	24.22195028208534	28.158051430540095	25.848994230841992
51	21.65166791794533	23.802655764279017	26.933275316487205	27.61240100128845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1696.0
1	2391.0
2	3086.0
3	4709.0
4	6332.0
5	4705.5
6	3079.0
7	3054.0
8	3029.0
9	3082.0
10	3135.0
11	3085.5
12	3036.0
13	3141.5
14	3247.0
15	3444.5
16	3642.0
17	3629.0
18	3616.0
19	3821.5
20	4027.0
21	4661.0
22	5295.0
23	5807.5
24	6320.0
25	7793.5
26	11756.0
27	14245.0
28	17683.0
29	21121.0
30	23551.5
31	25982.0
32	30303.5
33	34625.0
34	39248.0
35	43871.0
36	45879.0
37	47887.0
38	52063.0
39	56239.0
40	65239.0
41	74239.0
42	81649.5
43	89060.0
44	99832.5
45	110605.0
46	130064.0
47	149523.0
48	165248.5
49	180974.0
50	180485.0
51	179996.0
52	164486.0
53	148976.0
54	137873.0
55	126770.0
56	118745.0
57	110720.0
58	108569.5
59	106419.0
60	103966.5
61	101514.0
62	94750.0
63	87986.0
64	78700.0
65	69414.0
66	60408.0
67	51402.0
68	44198.0
69	36994.0
70	32020.5
71	27047.0
72	23333.0
73	19619.0
74	17320.5
75	12021.0
76	9020.0
77	7269.0
78	5518.0
79	4001.5
80	2485.0
81	1858.0
82	1231.0
83	1080.0
84	929.0
85	522.0
86	115.0
87	144.0
88	173.0
89	96.0
90	19.0
91	16.0
92	13.0
93	15.5
94	18.0
95	25.0
96	32.0
97	16.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2008612.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.59088894625136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25102192543302	16.773965904631325
2	10.175182318183431	4.597328275158849
3	3.9294161803408367	2.663070136610492
4	1.9800720914785095	1.789263548966507
5	1.1464963201985963	1.2950185523446167
6	0.7280786451981016	0.9868766290684475
7	0.5191539483258991	0.8209704434846815
8	0.41593415790427474	0.7517057896134439
9	0.32628346457289814	0.6633930161848035
>10	4.085846788763012	23.39429279602484
>50	2.0533293032401327	32.996442148865505
>100	0.38139671080693704	11.215646210071519
>500	0.005340442665825129	0.841652103316644
>1k	0.0024477028885031846	1.2103744456583738
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	4214	0.20979661577248368	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3384	0.1684745485937553	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3075	0.15309079105372267	No Hit
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	2484	0.12366748779754377	TruSeq Adapter, Index 16 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.957124621380336E-5	0.0	0.0	0.1335748267958172	0.0
2	9.957124621380336E-5	0.0	0.0	0.6706621288730725	0.0
3	1.4935686932070503E-4	0.0	0.0	0.9031112031591965	0.0
4	1.4935686932070503E-4	0.0	0.0	1.1703604279970448	0.0
5	1.4935686932070503E-4	0.0	0.0	1.982463512116825	0.0
6	1.4935686932070503E-4	0.0	0.0	2.7972052342612708	0.0
7	1.4935686932070503E-4	0.0	0.0	3.4794674133182517	0.0
8	1.4935686932070503E-4	0.0	0.0	4.618263756265521	0.0
9	1.4935686932070503E-4	0.0	0.0	5.134291739768557	0.0
10	1.4935686932070503E-4	0.0	0.0	6.089976560928641	0.0
11	1.4935686932070503E-4	0.0	0.0	7.063833134522745	0.0
12	1.9914249242760672E-4	0.0	0.0	7.890274478097313	0.0
13	1.9914249242760672E-4	0.0	0.0	8.238076841122128	0.0
14	1.9914249242760672E-4	0.0	0.0	8.384147859317778	0.0
15	1.9914249242760672E-4	0.0	0.0	8.613958295579236	0.0
16	1.9914249242760672E-4	0.0	0.0	9.071836671293411	0.0
17	1.9914249242760672E-4	0.0	0.0	9.625054515257302	0.0
18	1.9914249242760672E-4	0.0	0.0	10.29626428598455	0.0
19	1.9914249242760672E-4	0.0	0.0	10.64302115092412	0.0
20	1.9914249242760672E-4	0.0	0.0	10.99251622513457	0.0
21	2.4892811553450843E-4	0.0	0.0	11.40419354260554	0.0
22	2.4892811553450843E-4	0.0	0.0	11.860279635887867	0.0
23	2.4892811553450843E-4	0.0	0.0	12.341059398231216	0.0
24	2.4892811553450843E-4	0.0	0.0	12.722018986245228	0.0
25	2.9871373864141006E-4	0.0	0.0	13.058271084709242	0.0
26	2.9871373864141006E-4	0.0	0.0	13.362610598761732	0.0
27	3.4849936174831175E-4	0.0	0.0	13.680043731691336	0.0
28	3.4849936174831175E-4	0.0	0.0	14.01022198413631	0.0
29	3.9828498485521344E-4	0.0	0.0	14.359916200839187	0.0
30	3.9828498485521344E-4	0.0	0.0	14.765121387306259	0.0
31	4.480706079621151E-4	0.0	0.0	15.125668869846441	0.0
32	4.480706079621151E-4	0.0	0.0	15.51374780196474	0.0
33	4.480706079621151E-4	0.0	0.0	15.87499228322842	0.0
34	4.978562310690169E-4	0.0	0.0	16.236734620723166	0.0
35	4.978562310690169E-4	0.0	0.0	16.635368104940127	0.0
36	4.978562310690169E-4	0.0	0.0	16.97903826124707	0.0
37	4.978562310690169E-4	0.0	0.0	17.331620044090148	0.0
38	5.974274772828201E-4	0.0	0.0	17.69729544581034	0.0
39	5.974274772828201E-4	0.0	0.0	18.084179522974075	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	55	1.8189894E-12	45.000004	1
CGAATCG	25	3.8918522E-5	45.0	37
GTACGTA	20	7.0345483E-4	45.0	30
TCGATAG	55	6.184564E-11	40.909092	1
CGCATGG	350	0.0	39.214287	2
TATAGCG	35	6.2506224E-6	38.571426	1
TACGAAT	250	0.0	37.8	12
GTCGACA	30	1.14022565E-4	37.500004	17
TCGTAAG	60	1.5643309E-10	37.500004	1
TATACGC	30	1.14022565E-4	37.500004	39
TCGAAAC	30	1.14022565E-4	37.500004	39
CGGGATA	270	0.0	36.666664	6
CGTTCAT	230	0.0	36.195652	17
CATAGCG	25	0.002107483	36.0	3
GAACGTA	25	0.002107483	36.0	9
CGTACGA	25	0.002107483	36.0	2
TCGAACA	25	0.002107483	36.0	33
AGTACGG	320	0.0	35.859375	2
TACGGGA	515	0.0	35.82524	4
CGAATAT	265	0.0	35.660378	14
>>END_MODULE