FastQCFastQC Report
Sat 18 Jun 2016
SRR3552639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724778
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC14030.19357651584347207Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC13590.1875056913979177Illumina PCR Primer Index 7 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT13060.18019310740668176No Hit
GGCTATGGGAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATC12960.1788133745781467No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT10950.15108074472459154Illumina PCR Primer Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10350.14280234775338102No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10170.1403188286620179No Hit
GGCTATGGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG7750.10692929421146889No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7580.10458374840295924No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT7430.10251414916015664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTC207.031559E-445.00000438
AGCCGTC207.031559E-445.00000424
CAGCGAT207.031559E-445.00000421
CGACGAA207.031559E-445.00000431
CGCGAAA207.031559E-445.00000430
ACGATAT207.031559E-445.00000426
AAGCCGT207.031559E-445.00000423
TACGATA207.031559E-445.00000425
CGCTAGC207.031559E-445.00000412
ACTACGC207.031559E-445.00000412
TTGCGGA207.031559E-445.00000437
GATGCGA207.031559E-445.00000416
CGGTCTA302.1643864E-645.00000431
CCGCGAA207.031559E-445.00000429
TAGGACG207.031559E-445.0000041
TAATGCG302.1643864E-645.0000041
ATCGATC207.031559E-445.00000413
TACGTCG207.031559E-445.0000043
TGCGTAG302.1643864E-645.0000041
TCGATAG253.8893748E-545.01