##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724778
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.760227269591518	31.0	31.0	34.0	30.0	34.0
2	31.891048017461898	33.0	31.0	34.0	30.0	34.0
3	31.90284749261153	33.0	31.0	34.0	30.0	34.0
4	35.58137112329568	37.0	35.0	37.0	33.0	37.0
5	35.4019078945553	37.0	35.0	37.0	33.0	37.0
6	35.39871105359158	37.0	35.0	37.0	33.0	37.0
7	35.74542963500548	37.0	35.0	37.0	35.0	37.0
8	35.54885357999277	37.0	35.0	37.0	35.0	37.0
9	37.16746507206344	39.0	37.0	39.0	34.0	39.0
10	36.800465797802914	39.0	37.0	39.0	32.0	39.0
11	36.739913463156995	39.0	37.0	39.0	32.0	39.0
12	36.605988316422405	39.0	35.0	39.0	32.0	39.0
13	36.42663960550679	39.0	35.0	39.0	32.0	39.0
14	37.55900841361079	40.0	36.0	41.0	32.0	41.0
15	37.746130539282376	40.0	36.0	41.0	33.0	41.0
16	37.77154383825116	40.0	36.0	41.0	33.0	41.0
17	37.68503321016918	40.0	36.0	41.0	33.0	41.0
18	37.66852056767728	39.0	36.0	41.0	33.0	41.0
19	37.615010389388196	39.0	36.0	41.0	33.0	41.0
20	37.519154830858554	39.0	36.0	41.0	32.0	41.0
21	37.44824070267033	39.0	35.0	41.0	32.0	41.0
22	37.47586985256175	39.0	35.0	41.0	32.0	41.0
23	37.32745199219623	39.0	35.0	41.0	32.0	41.0
24	37.349882308789724	39.0	35.0	41.0	32.0	41.0
25	37.1628208913626	39.0	35.0	41.0	32.0	41.0
26	37.13031576565514	39.0	35.0	41.0	32.0	41.0
27	36.93027106231149	39.0	35.0	41.0	31.0	41.0
28	36.80594747633069	39.0	35.0	41.0	31.0	41.0
29	36.74850781894594	39.0	35.0	41.0	31.0	41.0
30	36.621147441009526	39.0	35.0	41.0	31.0	41.0
31	36.58849744335507	39.0	35.0	41.0	31.0	41.0
32	36.46591921940235	39.0	35.0	41.0	30.0	41.0
33	36.40386711517182	39.0	35.0	40.0	30.0	41.0
34	36.311456749515024	39.0	35.0	40.0	30.0	41.0
35	36.126880230912086	38.0	35.0	40.0	30.0	41.0
36	36.09812935823107	38.0	35.0	40.0	30.0	41.0
37	36.02462409179086	38.0	35.0	40.0	29.0	41.0
38	35.89795358026872	38.0	35.0	40.0	29.0	41.0
39	35.82323552867223	38.0	35.0	40.0	29.0	41.0
40	35.74705220081184	38.0	35.0	40.0	29.0	41.0
41	35.73839575704561	38.0	35.0	40.0	28.0	41.0
42	35.695308080543285	38.0	35.0	40.0	29.0	41.0
43	35.59559616875788	38.0	34.0	40.0	28.0	41.0
44	35.435777576030176	38.0	34.0	40.0	28.0	41.0
45	35.4036049659344	37.0	34.0	40.0	28.0	41.0
46	35.297732547069586	37.0	34.0	40.0	28.0	41.0
47	35.159967603873184	37.0	34.0	40.0	28.0	41.0
48	35.09463449497639	37.0	34.0	40.0	28.0	41.0
49	34.986648325418265	37.0	34.0	40.0	27.0	41.0
50	34.92200232347008	36.0	34.0	40.0	27.0	41.0
51	34.15496331290409	36.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	29.0
10	46.0
11	58.0
12	41.0
13	50.0
14	44.0
15	60.0
16	85.0
17	144.0
18	271.0
19	486.0
20	901.0
21	1757.0
22	3001.0
23	4121.0
24	5204.0
25	5815.0
26	6862.0
27	8180.0
28	9812.0
29	12013.0
30	14592.0
31	18395.0
32	24149.0
33	32510.0
34	53459.0
35	67473.0
36	66137.0
37	92664.0
38	134766.0
39	161565.0
40	80.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.11450402744013	18.080984798103692	23.235528672227908	13.56898250222827
2	32.46276790962198	24.86030205111082	24.50377908821736	18.173150951049838
3	31.577255380268166	24.41768375971677	24.95936686819964	19.045693991815423
4	28.075493461446126	26.300053257687182	25.950704905502096	19.673748375364593
5	24.45700614533002	31.643344582754995	23.63592713906879	20.263722132846198
6	25.09734015105315	35.351100612877325	24.90196998253258	14.649589253536947
7	82.02152935105647	6.787181730129778	7.059265043916896	4.132023874896865
8	82.67290121940788	5.687534665787317	6.679838516069749	4.959725598735061
9	77.31981379125746	8.084682482084169	8.735778403869874	5.859725322788495
10	40.57380328873117	26.702107403922305	15.778072733995788	16.946016573350736
11	31.811534014553423	27.692479628244786	20.900330859932286	19.595655497269508
12	27.817207475944354	22.60706036883018	29.53552674060195	20.040205414623514
13	25.818664473811292	25.501739843096782	28.091774308822842	20.587821374269087
14	20.931512821857176	29.771323080998595	27.328782054642943	21.96838204250129
15	19.691960848701257	25.965606020050274	33.39325972918604	20.949173402062424
16	21.374269086534085	25.319063216598735	30.151991368391425	23.154676328475755
17	22.09021245126094	23.58391121143302	26.53405594540673	27.791820391899314
18	25.049463421902985	24.044466029598027	27.897783873130805	23.00828667536818
19	25.680967137523492	26.584140247082555	25.257251185880364	22.47764142951359
20	26.22058064676356	24.70480616133492	25.168258418439855	23.906354773461665
21	26.3911156243705	25.961052901716116	25.827494763913915	21.820336709999474
22	22.935712728587237	26.241138665908732	24.38512206496334	26.438026540540687
23	21.9864565425551	27.19287837103223	25.634884061050418	25.185781025362246
24	22.416105345360922	25.962432634544648	27.600313475298645	24.021148544795786
25	23.184202611006405	27.48331213143887	25.64909530918433	23.683389948370397
26	21.50741330448772	26.9517010726043	25.46807436208053	26.072811260827457
27	22.40492950944979	26.583726327233993	26.535021758386705	24.47632240492951
28	22.38092215823328	26.351931212040103	28.175110171666358	23.09203645806026
29	23.196344259897515	26.57144670506003	26.42340137255822	23.808807662484234
30	24.342074400713045	25.69724798490021	26.235067841463177	23.72560977292357
31	26.954874458109934	27.463168032142253	22.491300784516085	23.090656725231725
32	26.978053969629322	26.20912886428672	24.242043770644255	22.57077339543971
33	24.941706287994393	26.302122856929984	22.762142338757524	25.994028516318103
34	24.008179056207556	25.558446862349577	25.618741186956555	24.81463289448631
35	24.35490591601842	25.592526263214392	25.27118648744857	24.781381333318617
36	26.33454657840056	26.74142978953555	24.714740237700372	22.209283394363517
37	25.063674670036896	27.32767826838011	26.17615324968473	21.432493811898265
38	23.90856234598732	27.886332090653966	25.614877935036663	22.59022762832205
39	23.39626754675225	25.923938088628518	25.92918107337695	24.750613291242285
40	23.56790631062201	26.386286559470623	27.264348531550354	22.781458598357013
41	21.307903937481544	26.623738579261513	26.597385682236492	25.470971801020447
42	23.17302677509527	26.188846791707256	26.60525015935914	24.032876273838333
43	24.2065846369509	24.328277072427696	26.899133251837114	24.56600503878429
44	23.916840742958534	25.32030497614442	26.230514723129012	24.532339557768033
45	23.32493535951698	24.93453167728601	27.06456321797847	24.675969745218538
46	24.13497650314993	26.333028872289173	26.032799008799934	23.499195615760964
47	22.815400025938978	26.27756361258206	27.647086418185985	23.25994994329298
48	22.88783599943707	25.475800865920323	27.3784524364702	24.257910698172406
49	23.677457097207697	24.684248142189745	27.940969510663955	23.697325249938604
50	21.879389275060777	24.396987767288742	28.228781778696373	25.494841178954108
51	21.970865561592653	24.486808374426374	26.8178669882364	26.724459075744573
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1228.0
1	1499.5
2	1771.0
3	2207.5
4	2644.0
5	1988.0
6	1332.0
7	1313.0
8	1294.0
9	1330.0
10	1366.0
11	1443.0
12	1520.0
13	1528.5
14	1537.0
15	1502.0
16	1467.0
17	1505.5
18	1544.0
19	1594.5
20	1645.0
21	1968.0
22	2291.0
23	2218.5
24	2146.0
25	2924.5
26	4577.5
27	5452.0
28	6813.5
29	8175.0
30	9178.0
31	10181.0
32	11710.0
33	13239.0
34	14902.0
35	16565.0
36	17674.0
37	18783.0
38	19854.5
39	20926.0
40	23344.0
41	25762.0
42	30259.5
43	34757.0
44	39840.5
45	44924.0
46	49774.5
47	54625.0
48	58709.5
49	62794.0
50	61859.5
51	60925.0
52	56879.0
53	52833.0
54	49439.0
55	46045.0
56	43427.0
57	40809.0
58	38131.0
59	35453.0
60	34384.0
61	33315.0
62	31823.0
63	30331.0
64	28066.5
65	25802.0
66	21454.5
67	17107.0
68	14867.0
69	12627.0
70	10840.0
71	9053.0
72	8048.5
73	7044.0
74	5920.5
75	3962.5
76	3128.0
77	2414.5
78	1701.0
79	1404.0
80	1107.0
81	873.5
82	640.0
83	419.0
84	198.0
85	181.0
86	164.0
87	93.0
88	22.0
89	12.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724778.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.986238797994346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.17186407317664	19.032483854207577
2	9.547919275530333	4.7713318208474815
3	3.3445157725800723	2.507006092720327
4	1.6207323113574823	1.619840182368135
5	0.976503712079164	1.2199577468568952
6	0.6125604156511294	0.9183348494194677
7	0.4731756812010479	0.827601639472512
8	0.38009560596365205	0.759772766134094
9	0.3135012417252456	0.7049895200293262
>10	4.60597491476633	30.569158490736125
>50	1.8328941934804919	30.521868677635176
>100	0.11024090228102577	4.400809459285455
>500	0.006124494571168099	0.9628248804360698
>1k	0.003897405636197881	1.1840200198513786
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1403	0.19357651584347207	Illumina PCR Primer Index 7 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1359	0.1875056913979177	Illumina PCR Primer Index 7 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1306	0.18019310740668176	No Hit
GGCTATGGGAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATC	1296	0.1788133745781467	No Hit
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	1095	0.15108074472459154	Illumina PCR Primer Index 7 (95% over 24bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1035	0.14280234775338102	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1017	0.1403188286620179	No Hit
GGCTATGGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	775	0.10692929421146889	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	758	0.10458374840295924	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	743	0.10251414916015664	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16142874093860465	0.0
2	0.0	0.0	0.0	0.7006283303301148	0.0
3	0.0	0.0	0.0	0.911589479813129	0.0
4	0.0	0.0	0.0	1.2234090990620576	0.0
5	0.0	0.0	0.0	1.9790887692507222	0.0
6	0.0	0.0	0.0	2.600658408505777	0.0
7	0.0	0.0	0.0	3.1223353909748917	0.0
8	0.0	0.0	0.0	4.0124010386628735	0.0
9	0.0	0.0	0.0	4.400243936764085	0.0
10	0.0	0.0	0.0	5.348534309816247	0.0
11	0.0	0.0	0.0	6.483916454417766	0.0
12	0.0	0.0	0.0	7.395919854079456	0.0
13	1.3797328285350825E-4	0.0	0.0	7.795490481223216	0.0
14	1.3797328285350825E-4	0.0	0.0	7.948502851907756	0.0
15	1.3797328285350825E-4	0.0	0.0	8.196578814478364	0.0
16	2.759465657070165E-4	0.0	0.0	8.777170388725928	0.0
17	2.759465657070165E-4	0.0	0.0	9.413503169246306	0.0
18	2.759465657070165E-4	0.0	0.0	10.313502893299741	0.0
19	2.759465657070165E-4	0.0	0.0	10.665610711141895	0.0
20	2.759465657070165E-4	0.0	0.0	11.080772319248101	0.0
21	2.759465657070165E-4	0.0	0.0	11.492760541848677	0.0
22	2.759465657070165E-4	0.0	0.0	11.95110778748803	0.0
23	2.759465657070165E-4	0.0	0.0	12.443534433992202	0.0
24	2.759465657070165E-4	0.0	0.0	12.806542141179781	0.0
25	4.1391984856052475E-4	0.0	0.0	13.104012539011945	0.0
26	4.1391984856052475E-4	0.0	0.0	13.394170352852873	0.0
27	4.1391984856052475E-4	0.0	0.0	13.785462583025422	0.0
28	4.1391984856052475E-4	0.0	0.0	14.108458038185486	0.0
29	4.1391984856052475E-4	0.0	0.0	14.421519416980097	0.0
30	4.1391984856052475E-4	0.0	0.0	14.780111979116365	0.0
31	4.1391984856052475E-4	0.0	0.0	15.087240506748273	0.0
32	4.1391984856052475E-4	0.0	0.0	15.40099175195715	0.0
33	4.1391984856052475E-4	0.0	0.0	15.730058031562768	0.0
34	4.1391984856052475E-4	0.0	0.0	16.02987397520344	0.0
35	4.1391984856052475E-4	0.0	0.0	16.395089254916677	0.0
36	4.1391984856052475E-4	0.0	0.0	16.710496179519797	0.0
37	4.1391984856052475E-4	0.0	0.0	17.013209562100396	0.0
38	5.51893131414033E-4	0.0	0.0	17.3123356393268	0.0
39	5.51893131414033E-4	0.0	0.0	17.616014834887373	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.031559E-4	45.000004	38
AGCCGTC	20	7.031559E-4	45.000004	24
CAGCGAT	20	7.031559E-4	45.000004	21
CGACGAA	20	7.031559E-4	45.000004	31
CGCGAAA	20	7.031559E-4	45.000004	30
ACGATAT	20	7.031559E-4	45.000004	26
AAGCCGT	20	7.031559E-4	45.000004	23
TACGATA	20	7.031559E-4	45.000004	25
CGCTAGC	20	7.031559E-4	45.000004	12
ACTACGC	20	7.031559E-4	45.000004	12
TTGCGGA	20	7.031559E-4	45.000004	37
GATGCGA	20	7.031559E-4	45.000004	16
CGGTCTA	30	2.1643864E-6	45.000004	31
CCGCGAA	20	7.031559E-4	45.000004	29
TAGGACG	20	7.031559E-4	45.000004	1
TAATGCG	30	2.1643864E-6	45.000004	1
ATCGATC	20	7.031559E-4	45.000004	13
TACGTCG	20	7.031559E-4	45.000004	3
TGCGTAG	30	2.1643864E-6	45.000004	1
TCGATAG	25	3.8893748E-5	45.0	1
>>END_MODULE