##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	945106
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79136625944603	31.0	31.0	34.0	30.0	34.0
2	31.94115157453238	33.0	31.0	34.0	30.0	34.0
3	31.96372787814277	33.0	31.0	34.0	30.0	34.0
4	35.60934223251149	37.0	35.0	37.0	33.0	37.0
5	35.431061701015544	37.0	35.0	37.0	33.0	37.0
6	35.4449754842314	37.0	35.0	37.0	33.0	37.0
7	35.78943737527854	37.0	35.0	37.0	35.0	37.0
8	35.54003677894332	37.0	35.0	37.0	35.0	37.0
9	37.276456820716405	39.0	37.0	39.0	34.0	39.0
10	36.88355062818351	39.0	37.0	39.0	32.0	39.0
11	36.83429583559939	39.0	37.0	39.0	33.0	39.0
12	36.71472300461536	39.0	35.0	39.0	33.0	39.0
13	36.56134549986986	39.0	35.0	39.0	32.0	39.0
14	37.68343762498598	40.0	36.0	41.0	33.0	41.0
15	37.786645095894	40.0	36.0	41.0	33.0	41.0
16	37.84071839560853	40.0	36.0	41.0	33.0	41.0
17	37.80699836843698	40.0	36.0	41.0	33.0	41.0
18	37.79318404496427	40.0	36.0	41.0	33.0	41.0
19	37.7451523956043	40.0	36.0	41.0	33.0	41.0
20	37.66381548736332	39.0	36.0	41.0	33.0	41.0
21	37.58463600908258	39.0	36.0	41.0	33.0	41.0
22	37.57996880773162	39.0	36.0	41.0	33.0	41.0
23	37.48844679856016	39.0	35.0	41.0	32.0	41.0
24	37.4980108051372	39.0	35.0	41.0	33.0	41.0
25	37.3406104712064	39.0	35.0	41.0	32.0	41.0
26	37.29827447926476	39.0	35.0	41.0	32.0	41.0
27	37.05197935469672	39.0	35.0	41.0	31.0	41.0
28	37.0830584082632	39.0	35.0	41.0	31.0	41.0
29	37.05393045859406	39.0	35.0	41.0	31.0	41.0
30	36.91520422047897	39.0	35.0	41.0	31.0	41.0
31	36.934037028650756	39.0	35.0	41.0	31.0	41.0
32	36.829187413898545	39.0	35.0	41.0	31.0	41.0
33	36.66566501535277	39.0	35.0	41.0	31.0	41.0
34	36.58759652356455	39.0	35.0	41.0	30.0	41.0
35	36.47979062665987	39.0	35.0	41.0	30.0	41.0
36	36.378640067886565	39.0	35.0	40.0	30.0	41.0
37	36.31279877601031	38.0	35.0	40.0	30.0	41.0
38	36.23007260561249	38.0	35.0	40.0	30.0	41.0
39	36.16579621756713	38.0	35.0	40.0	30.0	41.0
40	36.03402792914234	38.0	35.0	40.0	30.0	41.0
41	35.97161482415729	38.0	35.0	40.0	30.0	41.0
42	35.92341811394701	38.0	35.0	40.0	30.0	41.0
43	35.84375403393905	38.0	35.0	40.0	30.0	41.0
44	35.79718994483159	38.0	35.0	40.0	30.0	41.0
45	35.75325095809359	38.0	35.0	40.0	29.0	41.0
46	35.637868133309915	38.0	35.0	40.0	29.0	41.0
47	35.52688693120137	37.0	34.0	40.0	29.0	41.0
48	35.45804703387768	37.0	34.0	40.0	29.0	41.0
49	35.411908293884494	37.0	34.0	40.0	28.0	41.0
50	35.30949967516871	37.0	34.0	40.0	28.0	41.0
51	34.58373558098245	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	36.0
10	50.0
11	62.0
12	64.0
13	71.0
14	48.0
15	76.0
16	118.0
17	227.0
18	352.0
19	594.0
20	998.0
21	1687.0
22	2557.0
23	3867.0
24	5108.0
25	6215.0
26	7984.0
27	9905.0
28	11762.0
29	14221.0
30	18017.0
31	22944.0
32	30021.0
33	41115.0
34	67793.0
35	85574.0
36	88245.0
37	123631.0
38	181495.0
39	220141.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77775402970672	19.772596936216676	24.200248437741376	15.249400596335224
2	29.930822574399063	26.409630242533645	26.952214883833136	16.70733229923416
3	32.72214968479726	25.167441535658437	26.126381591059626	15.984027188484678
4	28.960455229360516	27.412692332923505	24.619672290727177	19.0071801469888
5	25.443071994040878	31.033556024403612	23.645813273854994	19.877558707700512
6	24.609197275226272	35.465016622474096	24.007254212754972	15.918531889544665
7	80.47224332508735	6.628674455563714	8.076025334724358	4.823056884624582
8	80.41203843801648	6.851718219966861	7.2751627859732135	5.4610805560434486
9	75.08628661758576	7.922180157569628	10.341697121804327	6.649836103040293
10	40.13930712533832	26.534907195594993	16.876202246097264	16.449583432969423
11	30.860982789232104	27.110609815195332	20.954792372495785	21.073615023076776
12	26.916028466648186	22.76167964228351	29.08192308587608	21.240368805192222
13	25.36773652902426	24.456727605157518	29.469604467647017	20.70593139817121
14	21.562343271548375	27.933692093796886	27.320639166400383	23.183325468254353
15	20.935006232105184	26.106172217719493	31.37066106870552	21.588160481469803
16	22.556834894710224	24.974553118909412	29.35628384540993	23.11232814097043
17	22.141537562982354	24.006301938618527	27.785983794410363	26.06617670398876
18	23.40933186330422	24.23580000550203	27.44041409111782	24.914454040075928
19	25.161727890839757	26.122255069801692	26.308371759358213	22.407645280000338
20	25.582633059148918	25.287745501562785	26.45914849762884	22.670472941659455
21	24.787907388165983	26.656586668585327	26.77519770269155	21.780308240557144
22	23.33949842663151	24.480428650331284	25.77224142053907	26.40783150249813
23	22.41790867902648	27.377775614587147	25.92513432355736	24.279181382829016
24	22.46255975520206	25.789488163232484	27.190283417944656	24.5576686636208
25	23.426261181285486	27.064900656645925	25.793403068015653	23.715435094052943
26	21.941030953141762	27.267311814759402	26.155584664577308	24.636072567521527
27	23.23601797047104	26.701343552998285	27.025328375864717	23.037310100665955
28	21.447964566937465	26.781969429884057	27.901949622582016	23.86811638059646
29	23.038685607751937	26.48993869470726	26.03327034216268	24.438105355378127
30	22.854367658230927	26.708644321377705	27.227951150452967	23.209036869938398
31	25.39641056135502	26.7872598417532	22.976152939458643	24.840176657433137
32	24.781029852736093	27.898352142511	23.664858756583918	23.65575924816899
33	23.62772006526252	25.677014006894467	25.209553214136825	25.48571271370619
34	22.842199710931897	26.31673061011146	26.093051996284018	24.748017682672632
35	25.11146897808288	24.16765950062744	25.78250481956521	24.938366701724462
36	25.30171218889733	26.72123550162627	25.177810742921956	22.79924156655444
37	24.33959788637465	26.341278121184292	27.221391039735227	22.097732952705833
38	24.16448525350596	26.46115885413911	25.661989237186095	23.712366655168836
39	25.593002266412444	23.921972773424358	26.96988485947608	23.51514010068712
40	25.95772326067129	23.236229586945804	28.443264565032916	22.36278258734999
41	22.432827640497468	24.817427886395812	27.3403194985536	25.40942497455312
42	22.765065505879765	24.838801150347155	27.92046606412402	24.475667279649056
43	23.688136568808154	23.922607622848655	28.25630140957734	24.13295439876585
44	23.464246338505944	24.113803107799548	27.223930437432415	25.198020116262093
45	24.387317401434334	23.730988904948227	26.038137521082295	25.84355617253514
46	24.405093185314662	24.667709230499014	26.574796901088344	24.35240068309798
47	23.241202574102797	25.083853028125947	28.269210014538054	23.405734383233202
48	23.363516896517428	24.479264759720074	27.735407456941335	24.42181088682116
49	22.481287813218835	24.156761252177002	29.354167680662275	24.007783253941888
50	22.506575981953347	24.018575694154944	28.45172922402355	25.02311909986816
51	22.484356252102938	24.12290261621448	26.44549923500644	26.94724189667614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1880.0
1	2350.0
2	2820.0
3	3374.5
4	3929.0
5	2944.0
6	1959.0
7	1893.5
8	1828.0
9	1874.0
10	1920.0
11	1985.0
12	2050.0
13	2076.5
14	2103.0
15	2160.5
16	2218.0
17	2242.0
18	2266.0
19	2398.5
20	2531.0
21	2804.0
22	3077.0
23	3277.0
24	3477.0
25	4057.5
26	5397.5
27	6157.0
28	8375.5
29	10594.0
30	12765.5
31	14937.0
32	14621.5
33	14306.0
34	17491.0
35	20676.0
36	21476.0
37	22276.0
38	24139.0
39	26002.0
40	30457.0
41	34912.0
42	39924.0
43	44936.0
44	52025.5
45	59115.0
46	68724.5
47	78334.0
48	82921.5
49	87509.0
50	86033.0
51	84557.0
52	75425.5
53	66294.0
54	60363.0
55	54432.0
56	51960.5
57	49489.0
58	47772.5
59	46056.0
60	44563.0
61	43070.0
62	40450.0
63	37830.0
64	33949.0
65	30068.0
66	26516.0
67	22964.0
68	19297.5
69	15631.0
70	13700.0
71	11769.0
72	10842.5
73	9916.0
74	8463.0
75	5437.5
76	3865.0
77	3338.5
78	2812.0
79	2119.5
80	1427.0
81	1192.5
82	958.0
83	596.5
84	235.0
85	154.5
86	74.0
87	126.5
88	179.0
89	93.0
90	7.0
91	7.0
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	945106.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.46886274694897
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8756959583378	18.321231274868495
2	10.103070985909513	4.944213145538046
3	3.676380900886934	2.6987057900792095
4	1.8506651233362619	1.8113468357392155
5	1.06960339604007	1.3085989345687488
6	0.727109693648873	1.067492837752223
7	0.5009981592787215	0.8581198637105582
8	0.41930260285324894	0.8207886270923683
9	0.2985061174584748	0.6573694695494444
>10	4.52831043877992	29.498946013331324
>50	1.7823125037057999	29.272419928529153
>100	0.1571604332511407	5.653152542740779
>500	0.005659516986883184	1.0032484246458886
>1k	0.005224169526353708	2.0843663118545446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	2846	0.30113024359172413	No Hit
GCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	2567	0.2716097453618959	No Hit
CTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGCT	2002	0.21182809124055926	Illumina Single End Adapter 2 (95% over 21bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1943	0.20558540523496835	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1788	0.18918512844061935	No Hit
TTGAATGATACCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGC	1663	0.17595909876775725	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1527	0.16156917848368332	No Hit
TTGAATCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCT	1089	0.11522517050997455	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGC	1082	0.11448451284829426	No Hit
TCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTCTGC	1031	0.10908829274176653	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCG	1028	0.10877086802961786	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGTGGTTCGTATGCCGTCTTC	1001	0.10591404562027963	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1161647476579347E-4	0.0	0.0	0.22505412091342136	0.0
2	2.1161647476579347E-4	0.0	0.0	1.0119499823300244	0.0
3	2.1161647476579347E-4	0.0	0.0	1.2984786891629088	0.0
4	2.1161647476579347E-4	0.0	0.0	1.6420380359451745	0.0
5	2.1161647476579347E-4	0.0	0.0	2.6990623274003127	0.0
6	2.1161647476579347E-4	0.0	0.0	3.576212615304527	0.0
7	2.1161647476579347E-4	0.0	0.0	4.425323720302273	0.0
8	2.1161647476579347E-4	0.0	0.0	5.404896381993131	0.0
9	2.1161647476579347E-4	0.0	0.0	5.86071826863865	0.0
10	2.1161647476579347E-4	0.0	0.0	6.902400365673269	0.0
11	2.1161647476579347E-4	0.0	0.0	8.073380128789786	0.0
12	2.1161647476579347E-4	0.0	0.0	9.375350489786332	0.0
13	2.1161647476579347E-4	0.0	0.0	9.790542013276818	0.0
14	2.1161647476579347E-4	0.0	0.0	10.008718598760352	0.0
15	3.174247121486902E-4	0.0	0.0	10.30424100577078	0.0
16	3.174247121486902E-4	0.0	0.0	10.878568118285145	0.0
17	3.174247121486902E-4	0.0	0.0	11.616792190505615	0.0
18	3.174247121486902E-4	0.0	0.0	12.424320658211883	0.0
19	3.174247121486902E-4	0.0	0.0	12.851680129001403	0.0
20	3.174247121486902E-4	0.0	0.0	13.241583483757378	0.0
21	3.174247121486902E-4	0.0	0.0	13.722481922662643	0.0
22	3.174247121486902E-4	0.0	0.0	14.197349292037083	0.0
23	3.174247121486902E-4	0.0	0.0	14.678459347417116	0.0
24	3.174247121486902E-4	0.0	0.0	15.067833660986174	0.0
25	3.174247121486902E-4	0.0	0.0	15.401235416979683	0.0
26	3.174247121486902E-4	0.0	0.0	15.716332347905949	0.0
27	3.174247121486902E-4	0.0	0.0	16.03566160832753	0.0
28	3.174247121486902E-4	0.0	0.0	16.373718926765886	0.0
29	3.174247121486902E-4	0.0	0.0	16.724684850164955	0.0
30	3.174247121486902E-4	0.0	0.0	17.113424314309718	0.0
31	3.174247121486902E-4	0.0	0.0	17.458464976415343	0.0
32	3.174247121486902E-4	0.0	0.0	17.814086462259258	0.0
33	3.174247121486902E-4	0.0	0.0	18.171400879901302	0.0
34	3.174247121486902E-4	0.0	0.0	18.517182199668607	0.0
35	3.174247121486902E-4	0.0	0.0	18.890791085867615	0.0
36	3.174247121486902E-4	0.0	0.0	19.232128459664843	0.0
37	4.2323294953158693E-4	0.0	0.0	19.583200191301295	0.0
38	4.2323294953158693E-4	0.0	0.0	19.911946384849955	0.0
39	4.2323294953158693E-4	0.0	0.0	20.256563814006046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCTA	25	3.890277E-5	45.000004	37
GTAATCG	30	2.1650903E-6	45.000004	1
CGATCCA	30	2.1650903E-6	45.000004	16
TATCCGG	25	3.890277E-5	45.000004	2
CGTGATA	20	7.03265E-4	45.0	43
TTCGCCT	20	7.03265E-4	45.0	23
ACGACGT	20	7.03265E-4	45.0	9
AATCGTT	45	3.8380676E-10	45.0	22
CACGCTT	60	3.6379788E-12	41.250004	32
TAACGCG	55	6.002665E-11	40.909092	1
GTATGCG	55	6.002665E-11	40.909092	1
CGAATAT	105	0.0	40.714287	14
TCGTTGA	50	1.0804797E-9	40.500004	24
ATATGCG	50	1.0804797E-9	40.500004	1
TATCGTG	50	1.0804797E-9	40.500004	1
CATACGA	150	0.0	40.5	18
CGTTCAT	45	1.927765E-8	40.0	17
AATAGCG	45	1.927765E-8	40.0	1
ATAGCGG	120	0.0	39.375004	2
GTTAGCG	40	3.4573532E-7	39.375	1
>>END_MODULE