##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552636_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	708046
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.76803202051844	31.0	31.0	34.0	30.0	34.0
2	31.918947356527685	33.0	31.0	34.0	30.0	34.0
3	31.955553735209293	33.0	31.0	34.0	30.0	34.0
4	35.582720614197385	37.0	35.0	37.0	33.0	37.0
5	35.411367340540025	37.0	35.0	37.0	33.0	37.0
6	35.39901362340865	37.0	35.0	37.0	33.0	37.0
7	35.772873513867744	37.0	35.0	37.0	35.0	37.0
8	35.60382941221333	37.0	35.0	37.0	35.0	37.0
9	37.30650409719143	39.0	37.0	39.0	34.0	39.0
10	36.848351660767804	39.0	37.0	39.0	32.0	39.0
11	36.79226208466682	39.0	37.0	39.0	32.0	39.0
12	36.636324476093364	39.0	35.0	39.0	32.0	39.0
13	36.473236202167655	39.0	35.0	39.0	32.0	39.0
14	37.599468113653636	40.0	36.0	41.0	33.0	41.0
15	37.71812283382718	40.0	36.0	41.0	33.0	41.0
16	37.77301615996701	40.0	36.0	41.0	33.0	41.0
17	37.733051242433405	40.0	36.0	41.0	33.0	41.0
18	37.70456721738418	39.0	36.0	41.0	33.0	41.0
19	37.675946195586164	39.0	36.0	41.0	33.0	41.0
20	37.6162424475246	39.0	36.0	41.0	33.0	41.0
21	37.51479423653265	39.0	35.0	41.0	32.0	41.0
22	37.5388929532827	39.0	35.0	41.0	33.0	41.0
23	37.443788962864	39.0	35.0	41.0	32.0	41.0
24	37.44588769656209	39.0	35.0	41.0	32.0	41.0
25	37.28700395172065	39.0	35.0	41.0	32.0	41.0
26	37.28310589989916	39.0	35.0	41.0	32.0	41.0
27	37.081771523319105	39.0	35.0	41.0	32.0	41.0
28	37.07218175090319	39.0	35.0	41.0	32.0	41.0
29	37.048248277654274	39.0	35.0	41.0	31.0	41.0
30	36.931248817167244	39.0	35.0	41.0	31.0	41.0
31	36.90731816859357	39.0	35.0	41.0	31.0	41.0
32	36.80049036362044	39.0	35.0	41.0	31.0	41.0
33	36.69442522096022	39.0	35.0	41.0	31.0	41.0
34	36.60399889272731	39.0	35.0	41.0	31.0	41.0
35	36.50559285696127	39.0	35.0	41.0	30.0	41.0
36	36.443201430415535	39.0	35.0	40.0	30.0	41.0
37	36.385818153057855	38.0	35.0	40.0	30.0	41.0
38	36.25738864424063	38.0	35.0	40.0	30.0	41.0
39	36.25974583572254	38.0	35.0	40.0	30.0	41.0
40	36.20197840253317	38.0	35.0	40.0	30.0	41.0
41	36.12537885956562	38.0	35.0	40.0	30.0	41.0
42	36.06128980320488	38.0	35.0	40.0	30.0	41.0
43	35.9962897890815	38.0	35.0	40.0	30.0	41.0
44	35.88498346152651	38.0	35.0	40.0	30.0	41.0
45	35.81803724616762	38.0	35.0	40.0	29.0	41.0
46	35.710000480194786	38.0	34.0	40.0	29.0	41.0
47	35.61966595390695	37.0	34.0	40.0	29.0	41.0
48	35.55247681647803	37.0	34.0	40.0	29.0	41.0
49	35.48486115308892	37.0	34.0	40.0	29.0	41.0
50	35.40868954841917	37.0	34.0	40.0	29.0	41.0
51	34.673413874239806	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	28.0
10	49.0
11	46.0
12	36.0
13	39.0
14	50.0
15	56.0
16	103.0
17	158.0
18	272.0
19	487.0
20	743.0
21	1084.0
22	1690.0
23	2386.0
24	3453.0
25	4576.0
26	5859.0
27	7288.0
28	8980.0
29	11085.0
30	13708.0
31	17583.0
32	22897.0
33	31209.0
34	52062.0
35	66456.0
36	63869.0
37	88676.0
38	134860.0
39	168165.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.65146614767967	19.04042957660943	24.32497323620217	13.983131039508734
2	31.020865875945912	24.96094886490426	24.965327111515354	19.052858147634474
3	32.19847298056906	24.66365179663468	25.927976430909855	17.209898791886403
4	28.72539354787683	27.33805430720603	24.722263807718708	19.214288337198433
5	24.80304951938151	30.176994150097592	24.61379627877285	20.406160051748053
6	24.51182550286281	35.9168754572443	23.813424551512192	15.757874488380699
7	82.06048759543872	6.5103114769379395	6.994037110583211	4.435163817040136
8	83.10378139273436	5.4383472260276875	6.456218946226658	5.001652435011285
9	77.66825884193965	7.4742319001872755	8.864395816091045	5.993113441782031
10	40.05431850472992	26.993443928784288	16.573640695661016	16.378596870824776
11	31.045158082949413	26.743177703143584	22.311121028859706	19.900543185047297
12	27.461210147363307	23.51400897681789	28.73909322275671	20.28568765306209
13	24.224132330385313	25.542973196656714	29.638752284456093	20.594142188501877
14	21.14806100168633	27.921349742813316	28.267937393898134	22.662651861602214
15	20.344158430384464	26.67383192617429	31.796804162441422	21.185205480999823
16	22.817020363083753	24.17357064371524	30.574002254090836	22.43540673911017
17	22.819562570793423	24.929736203579996	27.097109509834105	25.15359171579248
18	24.634557641735142	24.437536544235826	27.841976368767003	23.085929445262032
19	24.680458614270826	27.03510788847052	25.126051132271066	23.158382364987585
20	25.62757221988402	25.767676111439087	25.713018645681213	22.89173302299568
21	24.877197244246844	27.535781573513585	26.140250774667184	21.446770407572387
22	23.373763851501174	24.34658200173435	25.805809227084115	26.47384491968036
23	22.165509020600357	27.90454292517718	26.008762142572657	23.921185911649808
24	22.644715173872886	25.489445600992028	28.214833499518395	23.6510057256167
25	22.895970035845128	26.36114037788505	26.460851413608722	24.2820381726611
26	22.175960318962325	27.686336763430624	26.012434220375514	24.125268697231537
27	23.439861251952557	26.030088440581544	26.64318419989662	23.886866107569283
28	21.861291498010015	26.283885510263456	28.89317925671496	22.961643735011567
29	23.43957878442926	26.533869268380865	26.246741030949966	23.779810916239903
30	24.478635568875468	26.0659618160402	26.253802719032382	23.201599896051952
31	25.407106317951094	26.44362089468763	24.198851487050277	23.950421300311
32	25.55963878053121	27.435929304028267	24.44276219341681	22.561669722023712
33	25.16517288424763	26.351254014569676	23.995898571561735	24.487674529620957
34	23.807210265999668	26.267502393912263	26.104659866731822	23.82062747335625
35	24.434288167717916	25.660762153871357	25.263189114831526	24.6417605635792
36	26.219341681190205	27.740711761665203	24.1564813585558	21.88346519858879
37	24.162413176545027	27.427172810806077	25.827417992616297	22.582996020032596
38	23.236202167655772	28.731042898342764	25.932637145044247	22.100117788957217
39	24.302517068100098	26.434581933942148	24.742742703157706	24.520158294800055
40	24.38160797462312	24.244893693347606	27.479288068854284	23.89421026317499
41	21.366125929671238	25.514726444327064	27.02550399267844	26.09364363332326
42	22.683554458326157	25.95085630029687	26.993020227499343	24.37256901387763
43	23.680523581801182	24.751075495094952	27.003612759622964	24.564788163480905
44	23.37093917626821	25.156133923502143	27.234671193679503	24.238255706550138
45	24.434005700194618	25.09413230213856	25.689573841247604	24.782288156419217
46	23.92273948302794	25.738017021492954	26.42017609025402	23.919067405225082
47	22.485968425780246	25.892103055451198	27.964849741400982	23.65707877736757
48	22.03345545345924	25.897752405917128	27.81062247368109	24.258169666942546
49	23.26543755631696	25.33352352813235	27.920643574005076	23.480395341545606
50	21.852252537264526	24.72593588552156	28.205794538772906	25.21601703844101
51	21.645203842688186	25.052185874929027	26.50195043824836	26.800659844134422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1570.0
1	1840.0
2	2110.0
3	2334.0
4	2558.0
5	1914.0
6	1270.0
7	1283.0
8	1296.0
9	1413.0
10	1530.0
11	1566.5
12	1603.0
13	1521.5
14	1440.0
15	1494.5
16	1549.0
17	1408.5
18	1268.0
19	1649.5
20	2031.0
21	2151.0
22	2271.0
23	2203.0
24	2135.0
25	2725.5
26	4095.0
27	4874.0
28	7110.0
29	9346.0
30	9940.0
31	10534.0
32	11428.0
33	12322.0
34	13935.0
35	15548.0
36	16879.0
37	18210.0
38	18865.0
39	19520.0
40	22781.5
41	26043.0
42	29531.5
43	33020.0
44	37260.0
45	41500.0
46	47914.5
47	54329.0
48	59656.5
49	64984.0
50	64759.5
51	64535.0
52	59660.5
53	54786.0
54	49378.5
55	43971.0
56	41248.0
57	38525.0
58	36994.0
59	35463.0
60	34099.0
61	32735.0
62	30200.0
63	27665.0
64	25173.5
65	22682.0
66	19864.0
67	17046.0
68	14146.0
69	11246.0
70	9509.0
71	7772.0
72	6744.0
73	5716.0
74	4865.0
75	3398.5
76	2783.0
77	2171.5
78	1560.0
79	1151.5
80	743.0
81	559.5
82	376.0
83	271.5
84	167.0
85	100.0
86	33.0
87	39.5
88	46.0
89	24.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	708046.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.633153994695984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.94385723295596	19.210474334088705
2	9.908040496372445	5.079486556583978
3	3.553544758793915	2.7326568008762764
4	1.7249319963185377	1.768617899680458
5	0.9955888884196638	1.2760041646134719
6	0.676376796265858	1.0402602346273109
7	0.5257546800074757	0.9433725473244616
8	0.4053588568844049	0.8312500801305505
9	0.3481543948098625	0.8031865694482251
>10	5.31314763408359	35.803889102164035
>50	1.5007839252348878	24.6718199818478
>100	0.096681378374998	4.0592003918801804
>500	0.005000760950430931	0.9341858024681439
>1k	0.0027782005280171836	0.8455955342664081
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1326	0.18727596794558546	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1270	0.1793668772932832	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1248	0.1762597345370216	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.1542272677198939	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1001	0.14137499540990273	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	918	0.1296525931930976	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	897	0.12668668419848428	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	888	0.12541558034365	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.237012849447635E-4	0.0	0.0	0.1350194761357313	0.0
2	4.237012849447635E-4	0.0	0.0	0.6828652375693105	0.0
3	4.237012849447635E-4	0.0	0.0	0.9455600342350639	0.0
4	4.237012849447635E-4	0.0	0.0	1.2550032060063894	0.0
5	4.237012849447635E-4	0.0	0.0	2.1816379161805872	0.0
6	4.237012849447635E-4	0.0	0.0	2.9426054239413824	0.0
7	4.237012849447635E-4	0.0	0.0	3.5853602732025887	0.0
8	4.237012849447635E-4	0.0	0.0	4.678227120836782	0.0
9	4.237012849447635E-4	0.0	0.0	5.198814766272248	0.0
10	4.237012849447635E-4	0.0	0.0	6.304110184931488	0.0
11	4.237012849447635E-4	0.0	0.0	7.56207929993249	0.0
12	4.237012849447635E-4	0.0	0.0	8.577691279945089	0.0
13	4.237012849447635E-4	0.0	0.0	8.982608474590634	0.0
14	4.237012849447635E-4	0.0	0.0	9.136553274787232	0.0
15	4.237012849447635E-4	0.0	0.0	9.391762682085627	0.0
16	4.237012849447635E-4	0.0	0.0	9.95782759877183	0.0
17	4.237012849447635E-4	0.0	0.0	10.610610045110063	0.0
18	4.237012849447635E-4	0.0	0.0	11.41931456430797	0.0
19	4.237012849447635E-4	0.0	0.0	11.802905460944627	0.0
20	4.237012849447635E-4	0.0	0.0	12.20330317521743	0.0
21	5.64935046593018E-4	0.0	0.0	12.67177556260469	0.0
22	5.64935046593018E-4	0.0	0.0	13.127678145205255	0.0
23	5.64935046593018E-4	0.0	0.0	13.618606700694588	0.0
24	5.64935046593018E-4	0.0	0.0	13.990898896399386	0.0
25	9.886363315377814E-4	0.0	0.0	14.345254404374858	0.0
26	9.886363315377814E-4	0.0	0.0	14.646929719255528	0.0
27	9.886363315377814E-4	0.0	0.0	14.98250113693178	0.0
28	9.886363315377814E-4	0.0	0.0	15.315247879375068	0.0
29	9.886363315377814E-4	0.0	0.0	15.65336150476099	0.0
30	9.886363315377814E-4	0.0	0.0	16.03949460910732	0.0
31	9.886363315377814E-4	0.0	0.0	16.39286148075125	0.0
32	9.886363315377814E-4	0.0	0.0	16.74410984597046	0.0
33	9.886363315377814E-4	0.0	0.0	17.114989704058775	0.0
34	9.886363315377814E-4	0.0	0.0	17.45366826449129	0.0
35	0.001129870093186036	0.0	0.0	17.83980136883762	0.0
36	0.001129870093186036	0.0	0.0	18.19147343534177	0.0
37	0.0012711038548342904	0.0	0.0	18.527892255587915	0.0
38	0.0012711038548342904	0.0	0.0	18.879140620807124	0.0
39	0.001412337616482545	0.0	0.0	19.210898726918874	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	35	1.2110468E-7	45.0	1
AAATCGT	20	7.031449E-4	45.0	12
CAAACGT	20	7.031449E-4	45.0	34
GTCGATT	20	7.031449E-4	45.0	40
GTCGATG	20	7.031449E-4	45.0	1
GTCGAAC	20	7.031449E-4	45.0	41
TCCGATC	20	7.031449E-4	45.0	14
ACGTTAG	25	3.8892835E-5	45.0	1
ATTCGCG	25	3.8892835E-5	45.0	1
CGTCTAA	20	7.031449E-4	45.0	38
CCGCTAA	25	3.8892835E-5	45.0	33
AATATCG	25	3.8892835E-5	45.0	1
ACGATAG	25	3.8892835E-5	45.0	1
CTAACGG	35	1.2110468E-7	45.0	2
TCTACCG	25	3.8892835E-5	45.0	1
CGCTAAT	25	3.8892835E-5	45.0	34
TACTAAC	20	7.031449E-4	45.0	15
ATAACGG	40	6.8084773E-9	45.0	2
GCCCGTA	20	7.031449E-4	45.0	40
TAGTCGT	25	3.8892835E-5	45.0	16
>>END_MODULE