##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	804846
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718880382085516	31.0	31.0	34.0	30.0	34.0
2	31.85774794184229	33.0	31.0	34.0	30.0	34.0
3	31.90465380954866	33.0	31.0	34.0	30.0	34.0
4	35.554800545694455	37.0	35.0	37.0	33.0	37.0
5	35.35902768976922	37.0	35.0	37.0	33.0	37.0
6	35.37098028691203	37.0	35.0	37.0	33.0	37.0
7	35.76580737184505	37.0	35.0	37.0	35.0	37.0
8	35.55700966396056	37.0	35.0	37.0	35.0	37.0
9	37.2731367242926	39.0	37.0	39.0	34.0	39.0
10	36.840400523826915	39.0	37.0	39.0	32.0	39.0
11	36.77362750141021	39.0	37.0	39.0	32.0	39.0
12	36.64631618968101	39.0	35.0	39.0	32.0	39.0
13	36.450335840645295	39.0	35.0	39.0	32.0	39.0
14	37.559074655275666	39.0	36.0	41.0	32.0	41.0
15	37.700724113681375	40.0	36.0	41.0	33.0	41.0
16	37.74629432214361	40.0	36.0	41.0	33.0	41.0
17	37.74548422928113	40.0	36.0	41.0	33.0	41.0
18	37.718431849074236	39.0	36.0	41.0	33.0	41.0
19	37.68512485618367	39.0	36.0	41.0	33.0	41.0
20	37.626937327140844	39.0	36.0	41.0	33.0	41.0
21	37.54257211938681	39.0	36.0	41.0	33.0	41.0
22	37.5706035688815	39.0	36.0	41.0	33.0	41.0
23	37.46882881942633	39.0	35.0	41.0	32.0	41.0
24	37.472878041265034	39.0	35.0	41.0	33.0	41.0
25	37.313044980033446	39.0	35.0	41.0	32.0	41.0
26	37.31601449221342	39.0	35.0	41.0	32.0	41.0
27	37.09473116596219	39.0	35.0	41.0	32.0	41.0
28	37.139821779570255	39.0	35.0	41.0	32.0	41.0
29	37.138227685793304	39.0	35.0	41.0	32.0	41.0
30	36.99049259112923	39.0	35.0	41.0	31.0	41.0
31	36.97926311368883	39.0	35.0	41.0	31.0	41.0
32	36.89231231813291	39.0	35.0	41.0	31.0	41.0
33	36.800022613021625	39.0	35.0	41.0	31.0	41.0
34	36.720075641799795	39.0	35.0	41.0	31.0	41.0
35	36.62492700466922	39.0	35.0	41.0	31.0	41.0
36	36.5807160624517	39.0	35.0	41.0	31.0	41.0
37	36.535143369042025	39.0	35.0	40.0	30.0	41.0
38	36.44377185200647	39.0	35.0	40.0	30.0	41.0
39	36.410341854218075	39.0	35.0	40.0	30.0	41.0
40	36.309414471836845	38.0	35.0	40.0	30.0	41.0
41	36.22854931253929	38.0	35.0	40.0	30.0	41.0
42	36.228139296213186	38.0	35.0	40.0	30.0	41.0
43	36.18537832082162	38.0	35.0	40.0	30.0	41.0
44	36.113994478446806	38.0	35.0	40.0	30.0	41.0
45	36.03234656070851	38.0	35.0	40.0	30.0	41.0
46	35.911578115564964	38.0	35.0	40.0	30.0	41.0
47	35.85727952925156	38.0	35.0	40.0	30.0	41.0
48	35.816066676109465	38.0	35.0	40.0	30.0	41.0
49	35.787310864438666	38.0	35.0	40.0	30.0	41.0
50	35.71973644647547	37.0	35.0	40.0	30.0	41.0
51	35.0087954714318	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	27.0
10	41.0
11	46.0
12	49.0
13	57.0
14	51.0
15	72.0
16	103.0
17	188.0
18	316.0
19	508.0
20	845.0
21	1201.0
22	1930.0
23	2585.0
24	3718.0
25	4911.0
26	6409.0
27	7847.0
28	9514.0
29	11974.0
30	14919.0
31	19595.0
32	25489.0
33	35070.0
34	57831.0
35	73555.0
36	72600.0
37	101160.0
38	156469.0
39	195634.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.313876194949096	20.19044637110702	22.91456999227181	13.581107441672073
2	30.35823002164389	24.69764402134073	26.010317501733248	18.93380845528213
3	31.573493562743682	24.99496798145235	26.199297753856015	17.23224070194795
4	28.992502913600866	26.595149879604296	24.60681919273998	19.805528014054865
5	24.419951145933506	30.780546837531652	24.08771367441722	20.711788342117625
6	24.46517718917656	35.793431289961056	23.881960027135626	15.859431493726749
7	80.92728298332848	6.520874800893588	7.812923217609331	4.738918998168594
8	80.44010903949327	5.825959251832027	7.848457965871732	5.885473742802971
9	75.38088031747688	8.011967506827393	10.104044748933335	6.503107426762386
10	39.29745566232546	26.86886683912202	17.179559816412084	16.654117682140434
11	30.007603939138665	26.383556605860004	22.8933236917373	20.71551576326403
12	27.180354999589984	22.83480317973873	30.04872981912068	19.936112001550608
13	24.202766740469606	25.579054875094116	29.639831719359975	20.5783466650763
14	19.90915032192494	29.466879378166755	27.67026238559923	22.953707914309074
15	19.672210584385088	26.767480983939784	32.68028914848307	20.880019283192063
16	20.73315889002368	25.282476399211774	32.47465974857302	21.509704962191524
17	21.51666281499815	24.929738111390254	27.09586678693812	26.457732286673476
18	23.00315836818472	24.808348429388978	29.43233860887673	22.756154593549574
19	23.630359099753246	27.829547515922297	26.311120388248188	22.22897299607627
20	25.034354398232704	25.575575948690805	26.721633703838993	22.668435949237494
21	23.82132730982076	27.51892908705516	27.025170032527964	21.634573570596114
22	21.56325557932822	25.266199993539136	25.961115542600698	27.20942888453195
23	21.11397708381479	28.461469647609604	26.62969064889432	23.794862619681282
24	21.9235729568141	25.406351028643986	28.980699413303913	23.689376601238003
25	21.27326221413786	27.049522517351143	27.45009604321821	24.22711922529279
26	20.407009539713187	27.66479550125117	27.630378979332693	24.29781597970295
27	21.445220576358707	27.14022309858035	26.604344185098768	24.81021213996218
28	19.067374379695	26.995723405471356	29.520926984789636	24.41597523004401
29	21.90543284056826	24.662357767821423	29.09140382135216	24.340805570258162
30	22.309609540210175	25.736103552729343	27.52302925031621	24.431257656744272
31	23.204811852205268	27.141341324924273	23.746157650034913	25.907689172835546
32	22.63575889052067	27.11847980855965	25.568121106397996	24.677640194521686
33	23.079570501686035	25.30334995763165	25.15748354343564	26.459595997246677
34	20.757635622218412	25.83599843945301	27.395178704994493	26.011187233334077
35	20.775651491092706	24.42367856707991	27.803579815269007	26.99709012655837
36	23.70093160679186	25.778596153798368	26.315593293623873	24.2048789457859
37	21.236733486903084	27.55173039314353	27.11761007695882	24.09392604299456
38	21.12950800525815	27.81004067858944	26.30378979332692	24.756661522825485
39	22.669305680838324	25.542277653116248	28.014303357412473	23.774113308632955
40	23.647753731769804	24.650430020152925	28.716673748766848	22.985142499310427
41	18.969591698287623	25.607507523178345	28.178682629968964	27.244218148565068
42	21.133980910633834	24.257435583950222	27.69225417036303	26.916329335052918
43	21.678308645380607	23.60252768852675	28.77693372396707	25.94222994212557
44	21.736456415264534	23.789644230076313	28.18862241969271	26.28527693496644
45	22.300539482087256	24.15045859704838	28.048595631959405	25.50040628890496
46	23.086279859749567	25.898122125226443	26.519483230332263	24.49611478469173
47	20.23939983549648	24.944772043347424	29.28088106296111	25.534947058194984
48	20.142735380433027	24.004095193366183	29.32250393242931	26.530665493771476
49	21.137211342294055	22.825360379501173	30.55516210554566	25.482266172659116
50	20.6396006192489	24.139649075723803	30.08886172013031	25.131888584896988
51	20.558094343514163	23.456164284844554	27.32820937173074	28.65753199991054
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1874.0
1	2173.5
2	2473.0
3	2777.5
4	3082.0
5	2269.5
6	1457.0
7	1492.0
8	1527.0
9	1647.0
10	1767.0
11	1697.5
12	1628.0
13	1711.0
14	1794.0
15	1871.0
16	1948.0
17	1954.0
18	1960.0
19	1962.0
20	1964.0
21	2301.0
22	2638.0
23	2794.0
24	2950.0
25	3591.0
26	5590.0
27	6948.0
28	7468.0
29	7988.0
30	9832.5
31	11677.0
32	13140.0
33	14603.0
34	15823.5
35	17044.0
36	19874.5
37	22705.0
38	23011.5
39	23318.0
40	27598.0
41	31878.0
42	36360.0
43	40842.0
44	47365.5
45	53889.0
46	60664.0
47	67439.0
48	74042.5
49	80646.0
50	80173.0
51	79700.0
52	70488.5
53	61277.0
54	55728.0
55	50179.0
56	44864.5
57	39550.0
58	38030.5
59	36511.0
60	33805.5
61	31100.0
62	28043.5
63	24987.0
64	22739.0
65	20491.0
66	17959.0
67	15427.0
68	13774.5
69	12122.0
70	9965.5
71	7809.0
72	6920.5
73	6032.0
74	4863.5
75	3227.5
76	2760.0
77	2190.5
78	1621.0
79	1091.5
80	562.0
81	462.0
82	362.0
83	318.5
84	275.0
85	188.5
86	102.0
87	54.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	804846.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.920357969667712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20793932732118	18.24368054258559
2	10.96412402607034	5.464597911070144
3	4.382962824847861	3.276750076888642
4	2.1971173163033146	2.1901180009453696
5	1.2580558380524025	1.5675600915048091
6	0.856048738316902	1.2799824599003757
7	0.5983421924206667	1.0437631136435166
8	0.4282096284908488	0.8536909782440301
9	0.3600057494394393	0.8074324932452396
>10	3.7099124444659486	23.020003492175448
>50	1.749120377435678	30.564069168676415
>100	0.2801010742666204	9.392788629441009
>500	0.00453401019496328	0.8481719392718695
>1k	0.0035264523738603288	1.4473911024075463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	2950	0.36652974606322203	No Hit
CCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1873	0.2327153269072593	No Hit
CTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGCT	1604	0.1992927839611553	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1418	0.17618277285344028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1298	0.1612730882678177	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1287	0.15990636718080228	No Hit
TCTGTCTCTTATACACATCTGACGCTCCTGACTTCGTATGCCGTCTTCTGC	1099	0.13654786132999355	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	970	0.12051995040044927	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	963	0.11965021879962129	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	817	0.10151010255378047	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.21320848957440305	0.0
2	0.0	0.0	0.0	1.0982225171026507	0.0
3	0.0	0.0	0.0	1.45853989458853	0.0
4	0.0	0.0	0.0	1.8829689157925864	0.0
5	0.0	0.0	0.0	3.183217659030423	0.0
6	0.0	0.0	0.0	4.189994110674589	0.0
7	1.2424737154685493E-4	0.0	0.0	5.083705454211116	0.0
8	1.2424737154685493E-4	0.0	0.0	6.506462105794152	0.0
9	1.2424737154685493E-4	0.0	0.0	7.132544611018754	0.0
10	2.4849474309370987E-4	0.0	0.0	8.399495058682033	0.0
11	2.4849474309370987E-4	0.0	0.0	9.833433973704286	0.0
12	2.4849474309370987E-4	0.0	0.0	11.01602045608725	0.0
13	2.4849474309370987E-4	0.0	0.0	11.459832067252616	0.0
14	2.4849474309370987E-4	0.0	0.0	11.640611992853291	0.0
15	2.4849474309370987E-4	0.0	0.0	11.9463847742301	0.0
16	2.4849474309370987E-4	0.0	0.0	12.637572902145255	0.0
17	2.4849474309370987E-4	0.0	0.0	13.488667397241212	0.0
18	2.4849474309370987E-4	0.0	0.0	14.551727908196103	0.0
19	2.4849474309370987E-4	0.0	0.0	15.033310720311713	0.0
20	2.4849474309370987E-4	0.0	0.0	15.498368632011589	0.0
21	2.4849474309370987E-4	0.0	0.0	16.06642761472381	0.0
22	2.4849474309370987E-4	0.0	0.0	16.629143960459515	0.0
23	2.4849474309370987E-4	0.0	0.0	17.241062265327777	0.0
24	3.727421146405648E-4	0.0	0.0	17.714320503549747	0.0
25	3.727421146405648E-4	0.0	0.0	18.101226818546653	0.0
26	3.727421146405648E-4	0.0	0.0	18.483535980796326	0.0
27	3.727421146405648E-4	0.0	0.0	18.85466287960678	0.0
28	3.727421146405648E-4	0.0	0.0	19.254987910730748	0.0
29	3.727421146405648E-4	0.0	0.0	19.677801716104696	0.0
30	3.727421146405648E-4	0.0	0.0	20.162490712508976	0.0
31	3.727421146405648E-4	0.0	0.0	20.608041786875997	0.0
32	4.969894861874197E-4	0.0	0.0	21.01768537086598	0.0
33	4.969894861874197E-4	0.0	0.0	21.44037492886838	0.0
34	4.969894861874197E-4	0.0	0.0	21.85585813932106	0.0
35	6.212368577342746E-4	0.0	0.0	22.336943961950485	0.0
36	6.212368577342746E-4	0.0	0.0	22.74248738267942	0.0
37	6.212368577342746E-4	0.0	0.0	23.14492461911968	0.0
38	6.212368577342746E-4	0.0	0.0	23.552580245164915	0.0
39	6.212368577342746E-4	0.0	0.0	23.958247913265396	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	30	2.1646865E-6	45.000004	15
AAGTAAC	20	7.032024E-4	45.000004	23
TCACCCG	20	7.032024E-4	45.000004	19
GTCGAAC	20	7.032024E-4	45.000004	15
GACCGAA	20	7.032024E-4	45.000004	9
TAAACTA	20	7.032024E-4	45.000004	19
ATTGTCG	20	7.032024E-4	45.000004	14
ATTCGTT	20	7.032024E-4	45.000004	29
CATTCGT	20	7.032024E-4	45.000004	28
ATCGTTA	20	7.032024E-4	45.000004	11
TCTCGAC	30	2.1646865E-6	45.000004	20
CCGAACA	20	7.032024E-4	45.000004	41
TATACGG	40	6.8102963E-9	45.000004	2
GTTGCGT	30	2.1646865E-6	45.000004	27
GATACCC	25	3.889759E-5	45.0	19
TCAATCG	35	1.2113196E-7	45.0	13
TGACACG	25	3.889759E-5	45.0	30
AATTCGC	25	3.889759E-5	45.0	10
TCATCGA	25	3.889759E-5	45.0	16
TTCATCG	25	3.889759E-5	45.0	15
>>END_MODULE