##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552631_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	685704
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81576452813459	31.0	31.0	34.0	30.0	34.0
2	31.938601787360145	33.0	31.0	34.0	30.0	34.0
3	31.988040028933767	33.0	31.0	34.0	30.0	34.0
4	35.617991144867176	37.0	35.0	37.0	33.0	37.0
5	35.44814234713521	37.0	35.0	37.0	33.0	37.0
6	35.453969934549015	37.0	35.0	37.0	33.0	37.0
7	35.793001061682595	37.0	35.0	37.0	35.0	37.0
8	35.57057710032317	37.0	35.0	37.0	35.0	37.0
9	37.29098998984985	39.0	37.0	39.0	34.0	39.0
10	36.89842555971673	39.0	37.0	39.0	33.0	39.0
11	36.859830189119506	39.0	37.0	39.0	33.0	39.0
12	36.71782576738651	39.0	35.0	39.0	33.0	39.0
13	36.54347648548062	39.0	35.0	39.0	32.0	39.0
14	37.676563065112646	40.0	36.0	41.0	33.0	41.0
15	37.81549035735536	40.0	36.0	41.0	33.0	41.0
16	37.86072707757283	40.0	36.0	41.0	33.0	41.0
17	37.84562289267672	40.0	36.0	41.0	33.0	41.0
18	37.81669641711292	40.0	36.0	41.0	33.0	41.0
19	37.7821261068916	40.0	36.0	41.0	33.0	41.0
20	37.71429363107113	40.0	36.0	41.0	33.0	41.0
21	37.60803495385764	39.0	36.0	41.0	33.0	41.0
22	37.61484984774771	39.0	36.0	41.0	33.0	41.0
23	37.523483893925075	39.0	35.0	41.0	33.0	41.0
24	37.521455321829826	39.0	35.0	41.0	33.0	41.0
25	37.35994248247057	39.0	35.0	41.0	32.0	41.0
26	37.33163434951524	39.0	35.0	41.0	32.0	41.0
27	37.10658826549065	39.0	35.0	41.0	32.0	41.0
28	37.14982266400663	39.0	35.0	41.0	32.0	41.0
29	37.09159345723519	39.0	35.0	41.0	32.0	41.0
30	36.99549514076045	39.0	35.0	41.0	31.0	41.0
31	36.990589233838506	39.0	35.0	41.0	31.0	41.0
32	36.8656213176531	39.0	35.0	41.0	31.0	41.0
33	36.78691242868643	39.0	35.0	41.0	31.0	41.0
34	36.740853196131276	39.0	35.0	41.0	31.0	41.0
35	36.566897670131716	39.0	35.0	41.0	31.0	41.0
36	36.53737326893237	39.0	35.0	41.0	31.0	41.0
37	36.47531879644861	39.0	35.0	40.0	30.0	41.0
38	36.359121428488095	38.0	35.0	40.0	30.0	41.0
39	36.34797084456267	38.0	35.0	40.0	30.0	41.0
40	36.226777151657274	38.0	35.0	40.0	30.0	41.0
41	36.13623516852753	38.0	35.0	40.0	30.0	41.0
42	36.09639582093732	38.0	35.0	40.0	30.0	41.0
43	36.073929858947885	38.0	35.0	40.0	30.0	41.0
44	36.00765053142464	38.0	35.0	40.0	30.0	41.0
45	35.93689259505559	38.0	35.0	40.0	30.0	41.0
46	35.8143353400301	38.0	35.0	40.0	30.0	41.0
47	35.70358638712914	38.0	35.0	40.0	29.0	41.0
48	35.6388572911927	37.0	35.0	40.0	29.0	41.0
49	35.60370947230875	37.0	34.0	40.0	29.0	41.0
50	35.50752219616628	37.0	34.0	40.0	29.0	41.0
51	34.815787861817924	36.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	37.0
11	30.0
12	43.0
13	25.0
14	47.0
15	45.0
16	74.0
17	142.0
18	242.0
19	434.0
20	703.0
21	1131.0
22	1654.0
23	2342.0
24	3312.0
25	4250.0
26	5517.0
27	6862.0
28	8117.0
29	9946.0
30	12805.0
31	16252.0
32	21237.0
33	29353.0
34	48466.0
35	63028.0
36	63385.0
37	88454.0
38	132356.0
39	165313.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37935902371869	19.33297749466242	23.832732490987365	13.454930990631524
2	30.877317326426564	25.30727544246497	25.931743142813808	17.88366408829466
3	32.09358557045022	24.944582502070865	25.856346178526014	17.1054857489529
4	28.546428196422944	26.661649924748872	24.91935295696102	19.872568921867163
5	24.274759954732655	30.813878874849788	23.788835999206658	21.122525171210903
6	24.594139745429512	36.446484197262954	23.43372650589759	15.525649551409938
7	81.58374458950217	6.768372358918717	7.391235868538028	4.256647183041079
8	81.45030508790964	6.598036470547059	6.854852822792341	5.096805618750948
9	75.84599185654452	8.329104103228214	9.52174699287156	6.30315704735571
10	40.959947732549324	25.8494919090453	16.571727742582805	16.61883261582257
11	31.902395202594708	26.200809678811847	21.352945294179413	20.543849824414032
12	26.647503879224853	22.957281859227887	29.126707733949342	21.268506527597914
13	25.15925238878583	25.257983036412213	28.982913910375323	20.599850664426633
14	21.671450071751075	28.931433971509573	27.790416856252843	21.606699100486505
15	20.631788643496318	26.002181699392157	32.241316953087626	21.124712704023892
16	22.33048079054519	24.924019693628736	29.864489634011175	22.881009881814894
17	23.35307946285861	24.542513971042897	26.4573343600154	25.647072206083095
18	24.067819350623594	24.88872749757913	28.005232578488677	23.0382205733086
19	24.280010033483833	27.13094863089613	25.660051567440178	22.928989768179857
20	25.349567743516154	25.591363037112224	25.771324069861045	23.287745149510574
21	25.87195057925869	26.17353843640988	26.315144727170907	21.639366257160525
22	23.376267310676326	24.618056770851563	25.99591077199491	26.0097651464772
23	22.714757388027486	26.793193564570135	25.962076931153966	24.52997211624841
24	22.384002426703066	25.716635749536245	27.989628177756	23.909733646004693
25	22.862780441706626	26.518585278779184	26.358749547909888	24.25988473160431
26	21.42863392950894	27.459224388365826	26.337165890821694	24.774975791303536
27	22.48900400172669	26.45762603105713	27.231137633731173	23.822232333485
28	21.548073221098317	26.84569435208195	28.269632377818997	23.336600049000737
29	22.054414149545575	26.232310151318938	27.19001201684692	24.52326368228857
30	24.24967624514368	26.219622461003585	25.635551199934664	23.895150093918076
31	25.506778435009856	26.93596653949809	23.260619742629473	24.296635282862578
32	23.750627092739723	28.663242448636726	24.148903900225168	23.437226558398375
33	23.126305227911754	26.41533373000595	23.996943287482644	26.461417754599655
34	22.585838787581814	25.900388505827586	25.580571208568127	25.933201498022466
35	23.633521169484208	25.700593842240966	25.77686581965396	24.889019168620862
36	25.173106763268116	26.292102714874055	25.174710953997643	23.360079567860183
37	24.224446700033834	27.98291974379616	24.804142895476765	22.988490660693245
38	21.939495759103053	29.484733937675735	24.67653681471889	23.89923348850233
39	23.814211379837364	25.460986081457886	25.59223805023742	25.13256448846733
40	24.033256332178315	25.384859939565764	27.750895430098115	22.830988298157806
41	21.57869868048021	25.24442033297166	27.503120880146536	25.673760106401595
42	21.632366152158948	25.942826642399634	27.64137295392764	24.78343425151377
43	23.07147107206608	23.040699777163322	28.580407872784757	25.307421277985835
44	22.78548761564757	24.72072497754133	26.744776171642577	25.749011235168528
45	23.695063759289724	24.84410182819409	25.6922812175516	25.76855319496459
46	24.379324023193682	25.593842240966946	25.5789670178386	24.44786671800077
47	22.53712972361252	25.595154760654744	28.25840304271231	23.609312473020427
48	21.59269889048336	25.63015528566262	27.961627757749703	24.815518066104325
49	22.878530677960168	24.825580717044087	28.43194731254302	23.86394129245272
50	21.67130423623021	24.19323789856848	28.642825475715465	25.49263238948584
51	21.879265688985335	24.7850384422433	26.33235331863311	27.003342550138253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1247.0
1	1588.5
2	1930.0
3	2524.5
4	3119.0
5	2277.0
6	1435.0
7	1354.0
8	1273.0
9	1344.0
10	1415.0
11	1475.0
12	1535.0
13	1578.0
14	1621.0
15	1799.5
16	1978.0
17	1827.0
18	1676.0
19	1618.0
20	1560.0
21	1789.5
22	2019.0
23	2061.5
24	2104.0
25	2842.0
26	4815.5
27	6051.0
28	6755.5
29	7460.0
30	8330.0
31	9200.0
32	10568.5
33	11937.0
34	13408.5
35	14880.0
36	15710.0
37	16540.0
38	17114.0
39	17688.0
40	20819.5
41	23951.0
42	28442.5
43	32934.0
44	38628.5
45	44323.0
46	49555.5
47	54788.0
48	59330.5
49	63873.0
50	62900.5
51	61928.0
52	56219.0
53	50510.0
54	45431.0
55	40352.0
56	38406.0
57	36460.0
58	34460.0
59	32460.0
60	31462.0
61	30464.0
62	28716.0
63	26968.0
64	24781.0
65	22594.0
66	19538.0
67	16482.0
68	14559.5
69	12637.0
70	10861.5
71	9086.0
72	7494.5
73	5903.0
74	4979.5
75	3431.5
76	2807.0
77	2037.5
78	1268.0
79	1048.0
80	828.0
81	663.5
82	499.0
83	303.0
84	107.0
85	104.0
86	101.0
87	63.5
88	26.0
89	28.5
90	31.0
91	22.0
92	13.0
93	10.0
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	685704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.31995295595036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20571326560271	19.04205121903753
2	9.752397325959965	4.938604830020847
3	3.6434949841475115	2.7675936478156826
4	1.751504042242634	1.7739199980696154
5	1.0417226140363443	1.3188183790274937
6	0.6770287264096233	1.0285401301511188
7	0.4719448557834631	0.8364735082368044
8	0.3963710074660056	0.802887620971353
9	0.3338580999260669	0.7607944245681891
>10	4.967187902585468	32.92561546150072
>50	1.6406373852997351	27.120771780196712
>100	0.10655745813453978	4.269485569285933
>500	0.008686749304446177	1.4391936278648703
>1k	0.002895583101482059	0.9752498032531077
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	1746	0.2546288194322915	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1499	0.21860744577835334	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1332	0.1942529137937069	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1063	0.15502315868071354	No Hit
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	1011	0.14743971159567393	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	972	0.14175212628189424	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	886	0.12921027148740566	No Hit
CTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGCT	788	0.1149183904425233	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACGATTCGTATGCCGTCTTCTGC	748	0.10908496960787746	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	692	0.10091818043937327	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4583552086614633E-4	0.0	0.0	0.12235600200669677	0.0
2	1.4583552086614633E-4	0.0	0.0	0.6762393102563206	0.0
3	1.4583552086614633E-4	0.0	0.0	0.903013545203178	0.0
4	1.4583552086614633E-4	0.0	0.0	1.2053305799586993	0.0
5	1.4583552086614633E-4	0.0	0.0	2.162886609965816	0.0
6	1.4583552086614633E-4	0.0	0.0	2.892210049817414	0.0
7	1.4583552086614633E-4	0.0	0.0	3.5130318621445986	0.0
8	1.4583552086614633E-4	0.0	0.0	4.5920688810332155	0.0
9	1.4583552086614633E-4	0.0	0.0	5.0618050937430725	0.0
10	1.4583552086614633E-4	0.0	0.0	6.133696172109248	0.0
11	1.4583552086614633E-4	0.0	0.0	7.310442989978183	0.0
12	1.4583552086614633E-4	0.0	0.0	8.240290271020733	0.0
13	1.4583552086614633E-4	0.0	0.0	8.60210819828964	0.0
14	1.4583552086614633E-4	0.0	0.0	8.7349643577987	0.0
15	1.4583552086614633E-4	0.0	0.0	8.989301506189259	0.0
16	1.4583552086614633E-4	0.0	0.0	9.529184604435732	0.0
17	1.4583552086614633E-4	0.0	0.0	10.20104884906607	0.0
18	1.4583552086614633E-4	0.0	0.0	11.033769673211765	0.0
19	1.4583552086614633E-4	0.0	0.0	11.448234223513353	0.0
20	1.4583552086614633E-4	0.0	0.0	11.867219674961792	0.0
21	1.4583552086614633E-4	0.0	0.0	12.315809737146058	0.0
22	1.4583552086614633E-4	0.0	0.0	12.793421067982687	0.0
23	2.9167104173229266E-4	0.0	0.0	13.300637009555142	0.0
24	7.291776043307316E-4	0.0	0.0	13.698476310477991	0.0
25	7.291776043307316E-4	0.0	0.0	14.056356678683514	0.0
26	7.291776043307316E-4	0.0	0.0	14.354152812292185	0.0
27	7.291776043307316E-4	0.0	0.0	14.705762253100463	0.0
28	8.750131251968779E-4	0.0	0.0	15.031558806715434	0.0
29	8.750131251968779E-4	0.0	0.0	15.363917958769381	0.0
30	8.750131251968779E-4	0.0	0.0	15.75300712844026	0.0
31	8.750131251968779E-4	0.0	0.0	16.110304154562318	0.0
32	0.0010208486460630242	0.0	0.0	16.47547629881115	0.0
33	0.0010208486460630242	0.0	0.0	16.842398469310375	0.0
34	0.0010208486460630242	0.0	0.0	17.19925798886983	0.0
35	0.0010208486460630242	0.0	0.0	17.588930500624176	0.0
36	0.0010208486460630242	0.0	0.0	17.919247955386	0.0
37	0.0010208486460630242	0.0	0.0	18.28092004713404	0.0
38	0.0010208486460630242	0.0	0.0	18.61430004783405	0.0
39	0.0010208486460630242	0.0	0.0	18.961242751974613	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.031292E-4	45.000004	14
GTACCAT	20	7.031292E-4	45.000004	23
CGACGTA	20	7.031292E-4	45.000004	13
TCGATGT	20	7.031292E-4	45.000004	17
TCGATAG	20	7.031292E-4	45.000004	1
TCGTCTA	20	7.031292E-4	45.000004	18
AATTCGT	20	7.031292E-4	45.000004	42
AATTAGT	20	7.031292E-4	45.000004	36
CGTCGCA	20	7.031292E-4	45.000004	25
ACGTAGA	20	7.031292E-4	45.000004	15
ATTGCGA	20	7.031292E-4	45.000004	10
CGATAGA	30	2.1642154E-6	45.000004	10
ACGGTAT	20	7.031292E-4	45.000004	43
TATGGGT	190	0.0	45.0	4
CTACGTT	25	3.889154E-5	45.0	21
TTAACGG	45	3.8380676E-10	45.0	2
ACGTCCA	25	3.889154E-5	45.0	32
TTACGCG	50	2.1827873E-11	45.0	1
ATCGGGT	25	3.889154E-5	45.0	4
TGTCGTA	25	3.889154E-5	45.0	38
>>END_MODULE