FastQCFastQC Report
Sat 18 Jun 2016
SRR3552624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550371
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12240.22239543871315895No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC10880.19768483441169682RNA PCR Primer, Index 27 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.17315592573009841No Hit
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT7760.14099580101422496RNA PCR Primer, Index 38 (96% over 26bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC7560.13736188861695112RNA PCR Primer, Index 38 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6440.11701197919221762No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6110.11101602373671579No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC5790.10520176390107763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAAGG1100.045.0000042
GCGTAAG900.045.0000041
CGTGGTT207.0300786E-445.016
TAGAGTA253.888147E-545.05
TTCTACG207.0300786E-445.01
CGTATTA351.2103919E-745.017
ACGCATG207.0300786E-445.01
CGTTGCC207.0300786E-445.045
CCTACGC207.0300786E-445.010
ATATGCG207.0300786E-445.01
CTAAGAC253.888147E-545.027
TCGATTG253.888147E-545.01
TATTGCG253.888147E-545.01
ACTAGTC207.0300786E-445.032
TACGCAT207.0300786E-445.016
TACGAGC253.888147E-545.042
TCGCTAA207.0300786E-445.030
CGAATAT850.045.014
CTCGCTA207.0300786E-445.029
CGTACGA207.0300786E-445.040