##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552624_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 550371 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.726442708645624 31.0 31.0 34.0 30.0 34.0 2 31.875867369465325 33.0 31.0 34.0 30.0 34.0 3 31.905481938546906 33.0 31.0 34.0 30.0 34.0 4 35.54927312667274 37.0 35.0 37.0 33.0 37.0 5 35.36262085029916 37.0 35.0 37.0 33.0 37.0 6 35.35362873407211 37.0 35.0 37.0 32.0 37.0 7 35.73452634677336 37.0 35.0 37.0 35.0 37.0 8 35.546153412879676 37.0 35.0 37.0 35.0 37.0 9 37.24883760227192 39.0 37.0 39.0 34.0 39.0 10 36.804818931230024 39.0 37.0 39.0 32.0 39.0 11 36.738034889192924 39.0 37.0 39.0 32.0 39.0 12 36.594275497800574 39.0 35.0 39.0 32.0 39.0 13 36.4241575228346 39.0 35.0 39.0 32.0 39.0 14 37.556537317554884 39.0 36.0 41.0 32.0 41.0 15 37.70677960866397 40.0 36.0 41.0 33.0 41.0 16 37.74963070365263 40.0 36.0 41.0 33.0 41.0 17 37.72597393394638 40.0 36.0 41.0 33.0 41.0 18 37.682930241600666 39.0 36.0 41.0 33.0 41.0 19 37.6367595676371 39.0 36.0 41.0 33.0 41.0 20 37.55936813531236 39.0 36.0 41.0 33.0 41.0 21 37.44556308381074 39.0 35.0 41.0 32.0 41.0 22 37.474808810784 39.0 35.0 41.0 32.0 41.0 23 37.38117015613105 39.0 35.0 41.0 32.0 41.0 24 37.371638403912996 39.0 35.0 41.0 32.0 41.0 25 37.17419885858811 39.0 35.0 41.0 32.0 41.0 26 37.181103292142936 39.0 35.0 41.0 32.0 41.0 27 36.945954637871544 39.0 35.0 41.0 31.0 41.0 28 36.948934446037306 39.0 35.0 41.0 31.0 41.0 29 36.95804648137347 39.0 35.0 41.0 31.0 41.0 30 36.81413991652903 39.0 35.0 41.0 31.0 41.0 31 36.78519943819715 39.0 35.0 41.0 31.0 41.0 32 36.69432800783471 39.0 35.0 41.0 31.0 41.0 33 36.578035543297155 39.0 35.0 40.0 31.0 41.0 34 36.49003308677238 39.0 35.0 40.0 30.0 41.0 35 36.35315087459187 39.0 35.0 40.0 30.0 41.0 36 36.30854459991533 38.0 35.0 40.0 30.0 41.0 37 36.26029532806053 38.0 35.0 40.0 30.0 41.0 38 36.17396992210709 38.0 35.0 40.0 30.0 41.0 39 36.13091714498039 38.0 35.0 40.0 30.0 41.0 40 35.984494095800834 38.0 35.0 40.0 30.0 41.0 41 35.90462070130875 38.0 35.0 40.0 30.0 41.0 42 35.855301242252956 38.0 35.0 40.0 29.0 41.0 43 35.763437753806066 38.0 35.0 40.0 29.0 41.0 44 35.67905830794137 38.0 35.0 40.0 29.0 41.0 45 35.626313886451136 38.0 34.0 40.0 29.0 41.0 46 35.525222077471376 38.0 34.0 40.0 29.0 41.0 47 35.42622521898865 37.0 34.0 40.0 28.0 41.0 48 35.385349155387914 37.0 34.0 40.0 28.0 41.0 49 35.33769039429767 37.0 34.0 40.0 28.0 41.0 50 35.23824656459007 37.0 34.0 40.0 28.0 41.0 51 34.527596839222994 36.0 33.0 40.0 26.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 20.0 10 28.0 11 40.0 12 32.0 13 32.0 14 40.0 15 57.0 16 84.0 17 147.0 18 254.0 19 395.0 20 673.0 21 1066.0 22 1492.0 23 2137.0 24 3003.0 25 3790.0 26 4803.0 27 6214.0 28 7291.0 29 8914.0 30 11111.0 31 14052.0 32 17983.0 33 24476.0 34 40156.0 35 52602.0 36 50866.0 37 70198.0 38 102081.0 39 126273.0 40 54.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94230618982468 20.11188816271206 23.247046083460067 13.69875956400319 2 31.032703394619265 24.78655307056513 26.169801824587413 18.01094171022819 3 31.912110194759535 24.82707119379473 25.958671514305802 17.30214709713993 4 27.79652270922705 27.870472826511573 24.938087217531447 19.394917246729936 5 25.250967074936725 30.462724235106865 23.88661466538026 20.39969402457615 6 24.46767725770435 35.85381497208247 24.206217260720493 15.472290509492687 7 81.65873565285962 6.717832153220282 7.044157486495473 4.579274707424628 8 82.58556500978432 5.8373351793608315 6.622078561552117 4.955021249302742 9 76.90503315036584 7.945549456639249 9.336611122315674 5.812806270679233 10 40.19761215616376 26.749047460712866 16.941662987330364 16.11167739579302 11 31.87250054962925 26.238301073276027 21.98589678598618 19.903301591108544 12 26.971079508186296 23.487792779779458 29.47684380172647 20.064283910307772 13 23.683115571132927 25.987561117864132 29.904191899645877 20.425131411357068 14 21.3292488157988 29.046225182649522 27.646078735979913 21.97844726557177 15 19.633483595610958 25.993920464559363 33.06369703345561 21.30889890637406 16 22.585855722776092 23.918411398856406 31.52110122081287 21.97463165755463 17 22.930714009277377 24.292704375775614 26.492493245465333 26.28408836948168 18 23.947482698034598 24.39427222727942 28.843634566501503 22.81461050818448 19 24.93826891315131 26.748502373853274 25.785697284195567 22.527531428799847 20 26.142365785987998 25.764075505431787 25.3767004438824 22.716858264697812 21 24.652643398725587 27.23998902558456 26.88313882817227 21.224228747517586 22 23.11349980286025 25.390327615372176 25.560213019944726 25.93595956182284 23 21.996798523178 27.79179862311059 26.271733067330942 23.93966978638046 24 23.140572450219942 25.014762769113926 28.72716767416888 23.117497106497254 25 22.311677032401782 26.326786840149648 27.446395249749717 23.915140877698864 26 21.885600803821422 27.793433883689367 25.621262748218932 24.699702564270286 27 23.77977764090041 26.781025889808873 26.42453908363631 23.014657385654406 28 22.081105290794756 26.71198155426067 28.622329301507527 22.584583853437046 29 23.305552073056173 25.83148458040122 25.89943874223024 24.963524604312365 30 23.542664856978295 27.181119644748726 26.26900763303299 23.00720786523999 31 24.85450723239415 27.599019570435217 23.805941810160782 23.740531387009852 32 25.432117607940825 28.017827974221028 23.69946817692066 22.85058624091749 33 24.802723980732996 26.633852437719284 24.25763712114192 24.3057864604058 34 22.86984597662304 26.938010905371108 25.7250109471611 24.467132170844756 35 23.27720755635744 26.29353654171459 26.018994460100554 24.410261441827423 36 26.33405466494419 27.19220307756041 24.703154781047694 21.770587476447705 37 24.15225366162098 28.23259219689991 25.82766897238408 21.787485169095028 38 22.672706229070936 27.798884752285275 26.034983674648554 23.493425343995234 39 24.338491671981263 25.480448642824566 26.188879864673105 23.992179820521066 40 24.224386822706865 25.16193622120352 28.122484651262514 22.491192304827106 41 22.242996088093303 25.447743431249105 27.48982050289714 24.819439977760457 42 23.971829911096336 25.572931713335183 26.40037356619444 24.054864809374042 43 24.108828408473556 24.51110251085177 27.170762994416492 24.209306086258177 44 23.54357333507761 25.002952553822784 27.06410766555651 24.389366445543097 45 23.2753906001588 25.663416132027304 26.16271569541273 24.898477572401163 46 24.021251119699258 26.05969427895002 26.695628948472937 23.223425652877786 47 23.016656037472906 26.289720933697453 27.583393747126937 23.110229281702708 48 22.55660272797804 25.21789847212153 27.527794887448646 24.697703912451782 49 23.520134600115195 24.735314905763566 27.74455776194603 23.999992732175205 50 21.45298353292597 25.2645942464265 28.7035472435866 24.578874977060927 51 21.944106793417532 25.238611772785998 26.123287745902307 26.693993687894167 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1436.0 1 1666.5 2 1897.0 3 2095.5 4 2294.0 5 1800.0 6 1306.0 7 1267.5 8 1229.0 9 1289.5 10 1350.0 11 1396.5 12 1443.0 13 1419.0 14 1395.0 15 1420.0 16 1445.0 17 1565.0 18 1685.0 19 1586.5 20 1488.0 21 1624.0 22 1760.0 23 1753.5 24 1747.0 25 2583.0 26 3606.5 27 3794.0 28 4511.0 29 5228.0 30 6616.5 31 8005.0 32 8902.5 33 9800.0 34 10547.5 35 11295.0 36 12445.5 37 13596.0 38 15755.5 39 17915.0 40 20045.0 41 22175.0 42 25802.5 43 29430.0 44 32362.5 45 35295.0 46 38334.0 47 41373.0 48 45163.5 49 48954.0 50 48999.0 51 49044.0 52 44335.0 53 39626.0 54 35945.5 55 32265.0 56 30095.5 57 27926.0 58 26468.5 59 25011.0 60 24440.0 61 23869.0 62 22240.5 63 20612.0 64 18774.0 65 16936.0 66 14733.0 67 12530.0 68 10835.5 69 9141.0 70 7980.0 71 6819.0 72 6236.0 73 5653.0 74 5079.5 75 3473.0 76 2440.0 77 2084.0 78 1728.0 79 1319.5 80 911.0 81 686.5 82 462.0 83 273.5 84 85.0 85 60.0 86 35.0 87 23.5 88 12.0 89 7.0 90 2.0 91 1.0 92 0.0 93 1.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 550371.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.29693272154041 #Duplication Level Percentage of deduplicated Percentage of total 1 76.04592796492388 19.997746514407115 2 9.592174021609676 5.044895097991547 3 3.362167519304028 2.652440791610594 4 1.6928097058205138 1.7806281177733065 5 0.9711888347447538 1.276964372359701 6 0.6723611019441222 1.0608620797443216 7 0.47637648382875186 0.8769068241758071 8 0.38291433785757384 0.8055578064603051 9 0.3105559381688884 0.735000175107191 >10 4.820829760475812 33.64900364067844 >50 1.5789178634114764 27.13586693814359 >100 0.08821934388616698 3.772848536138306 >500 0.004167843018244109 0.7889480404561512 >1k 0.0013892810060813698 0.42233106495360534 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1224 0.22239543871315895 No Hit GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC 1088 0.19768483441169682 RNA PCR Primer, Index 27 (95% over 23bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 953 0.17315592573009841 No Hit CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT 776 0.14099580101422496 RNA PCR Primer, Index 38 (96% over 26bp) CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC 756 0.13736188861695112 RNA PCR Primer, Index 38 (96% over 25bp) GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 644 0.11701197919221762 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 611 0.11101602373671579 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC 579 0.10520176390107763 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.633912397273839E-4 0.0 0.0 0.14953549514781847 0.0 2 3.633912397273839E-4 0.0 0.0 0.6700934460572959 0.0 3 3.633912397273839E-4 0.0 0.0 0.9395480503151511 0.0 4 3.633912397273839E-4 0.0 0.0 1.27513986020339 0.0 5 3.633912397273839E-4 0.0 0.0 2.239398514820003 0.0 6 3.633912397273839E-4 0.0 0.0 3.033590069244201 0.0 7 3.633912397273839E-4 0.0 0.0 3.6889661700925376 0.0 8 3.633912397273839E-4 0.0 0.0 4.860539526973623 0.0 9 3.633912397273839E-4 0.0 0.0 5.401992474167425 0.0 10 3.633912397273839E-4 0.0 0.0 6.412946903089007 0.0 11 3.633912397273839E-4 0.0 0.0 7.646115075103885 0.0 12 3.633912397273839E-4 0.0 0.0 8.583119386740943 0.0 13 3.633912397273839E-4 0.0 0.0 8.949417756386147 0.0 14 3.633912397273839E-4 0.0 0.0 9.087324731862688 0.0 15 3.633912397273839E-4 0.0 0.0 9.355689162401362 0.0 16 3.633912397273839E-4 0.0 0.0 9.890601067280071 0.0 17 3.633912397273839E-4 0.0 0.0 10.556878905320229 0.0 18 3.633912397273839E-4 0.0 0.0 11.377961411484254 0.0 19 3.633912397273839E-4 0.0 0.0 11.745713346088365 0.0 20 3.633912397273839E-4 0.0 0.0 12.132179929538438 0.0 21 3.633912397273839E-4 0.0 0.0 12.570429764649663 0.0 22 3.633912397273839E-4 0.0 0.0 13.003410426784841 0.0 23 3.633912397273839E-4 0.0 0.0 13.449836564789933 0.0 24 3.633912397273839E-4 0.0 0.0 13.799964024267267 0.0 25 5.450868595910758E-4 0.0 0.0 14.125199183823275 0.0 26 7.267824794547678E-4 0.0 0.0 14.432446477012778 0.0 27 7.267824794547678E-4 0.0 0.0 14.74696159499683 0.0 28 7.267824794547678E-4 0.0 0.0 15.057479409343879 0.0 29 7.267824794547678E-4 0.0 0.0 15.364545006913518 0.0 30 7.267824794547678E-4 0.0 0.0 15.736839332014224 0.0 31 7.267824794547678E-4 0.0 0.0 16.07479318496069 0.0 32 7.267824794547678E-4 0.0 0.0 16.415654167824975 0.0 33 7.267824794547678E-4 0.0 0.0 16.732894720106984 0.0 34 9.084780993184597E-4 0.0 0.0 17.042685751974577 0.0 35 9.084780993184597E-4 0.0 0.0 17.418068902612966 0.0 36 9.084780993184597E-4 0.0 0.0 17.745847800847066 0.0 37 9.084780993184597E-4 0.0 0.0 18.068357526105117 0.0 38 9.084780993184597E-4 0.0 0.0 18.39758998929813 0.0 39 9.084780993184597E-4 0.0 0.0 18.724278713813046 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTAAGG 110 0.0 45.000004 2 GCGTAAG 90 0.0 45.000004 1 CGTGGTT 20 7.0300786E-4 45.0 16 TAGAGTA 25 3.888147E-5 45.0 5 TTCTACG 20 7.0300786E-4 45.0 1 CGTATTA 35 1.2103919E-7 45.0 17 ACGCATG 20 7.0300786E-4 45.0 1 CGTTGCC 20 7.0300786E-4 45.0 45 CCTACGC 20 7.0300786E-4 45.0 10 ATATGCG 20 7.0300786E-4 45.0 1 CTAAGAC 25 3.888147E-5 45.0 27 TCGATTG 25 3.888147E-5 45.0 1 TATTGCG 25 3.888147E-5 45.0 1 ACTAGTC 20 7.0300786E-4 45.0 32 TACGCAT 20 7.0300786E-4 45.0 16 TACGAGC 25 3.888147E-5 45.0 42 TCGCTAA 20 7.0300786E-4 45.0 30 CGAATAT 85 0.0 45.0 14 CTCGCTA 20 7.0300786E-4 45.0 29 CGTACGA 20 7.0300786E-4 45.0 40 >>END_MODULE