SRR3552623_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552623_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	587077
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	13527	2.304127056587126	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	12827	2.1848922713715577	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	11293	1.923597756342013	No Hit
GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	3990	0.6796382757287375	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT	3591	0.6116744481558637	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACACCACT	2754	0.46910371211953455	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTC	2302	0.3921121079517679	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACACCACTCGTA	2138	0.36417710112983476	No Hit
GAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT	2015	0.34322584601338496	No Hit
CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1994	0.3396488024569179	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT	1658	0.28241610555344526	Illumina Single End Adapter 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1502	0.25584378199111873	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1388	0.2364255455417262	No Hit
TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	1060	0.18055553189786008	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT	904	0.1539832083355335	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	890	0.15159851263122215	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCACTCGAATGC	829	0.14120805277672263	No Hit
ACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC	750	0.12775155558810855	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC	731	0.12451518284654313	No Hit
AAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC	676	0.1151467354367485	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACACCAC	676	0.1151467354367485	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC	640	0.10901466076851929	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG	625	0.10645962965675712	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATG	602	0.10254191528538846	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGC	35	1.2105738E-7	45.000004	8
TTTCGCA	35	1.2105738E-7	45.000004	13
CGCATAC	35	1.2105738E-7	45.000004	16
ACGGGTT	35	1.2105738E-7	45.000004	5
ATTTTCG	35	1.2105738E-7	45.000004	11
AGCGCAG	35	1.2105738E-7	45.000004	1
CGTCAGG	35	1.2105738E-7	45.000004	2
AACCGAT	25	3.8884657E-5	45.0	16
TCCATAC	20	7.030464E-4	45.0	18
GTCGAGC	20	7.030464E-4	45.0	16
AGTCACG	20	7.030464E-4	45.0	2
GAATGCG	25	3.8884657E-5	45.0	1
TCCGAGC	20	7.030464E-4	45.0	23
ACGCAAT	20	7.030464E-4	45.0	27
GACGTAG	20	7.030464E-4	45.0	1
GGCCGTA	25	3.8884657E-5	45.0	42
GACCGCA	20	7.030464E-4	45.0	21
TTACTCG	20	7.030464E-4	45.0	26
CGCGACT	75	0.0	45.0	24
CCCGTTG	20	7.030464E-4	45.0	26

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content