##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552623_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 587077 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.45310410729768 31.0 31.0 34.0 30.0 34.0 2 31.605486162803174 31.0 31.0 34.0 30.0 34.0 3 31.680687541838633 31.0 31.0 34.0 30.0 34.0 4 35.405822404897485 37.0 35.0 37.0 33.0 37.0 5 35.152962899244905 37.0 35.0 37.0 33.0 37.0 6 35.15754151499718 37.0 35.0 37.0 32.0 37.0 7 35.571892613745725 37.0 35.0 37.0 35.0 37.0 8 35.08318670293675 37.0 35.0 37.0 32.0 37.0 9 36.97331184836061 39.0 37.0 39.0 33.0 39.0 10 36.47213227566401 38.0 35.0 39.0 32.0 39.0 11 36.432784796542876 38.0 35.0 39.0 32.0 39.0 12 36.3864569724244 38.0 35.0 39.0 32.0 39.0 13 36.03897614793289 38.0 35.0 39.0 31.0 39.0 14 37.09242058537466 39.0 36.0 40.0 31.0 41.0 15 37.13852016004715 39.0 36.0 40.0 31.0 41.0 16 37.39350204487657 39.0 36.0 40.0 32.0 41.0 17 37.21009169154983 39.0 35.0 41.0 32.0 41.0 18 37.236527746786194 39.0 36.0 40.0 32.0 41.0 19 37.203480974386665 39.0 36.0 40.0 32.0 41.0 20 36.84207693369013 39.0 35.0 40.0 31.0 41.0 21 37.01559250319805 39.0 35.0 40.0 31.0 41.0 22 37.06756694607351 39.0 35.0 40.0 31.0 41.0 23 36.7205477305362 39.0 35.0 40.0 31.0 41.0 24 36.83326888977085 39.0 35.0 40.0 31.0 41.0 25 36.879063564064 39.0 35.0 40.0 31.0 41.0 26 36.844778453252296 39.0 35.0 40.0 31.0 41.0 27 36.41601868238749 38.0 35.0 40.0 30.0 41.0 28 36.49931610333908 39.0 35.0 40.0 30.0 41.0 29 36.52700071711207 39.0 35.0 40.0 30.0 41.0 30 36.46648395355294 38.0 35.0 40.0 30.0 41.0 31 36.539430091793754 39.0 35.0 40.0 30.0 41.0 32 36.0098624200914 38.0 35.0 40.0 29.0 41.0 33 35.870764823013 38.0 35.0 40.0 28.0 41.0 34 35.7959603254769 38.0 35.0 40.0 28.0 41.0 35 35.52088737933865 38.0 35.0 40.0 27.0 41.0 36 35.58674075121321 38.0 35.0 40.0 27.0 41.0 37 35.39055353897359 38.0 35.0 40.0 26.0 41.0 38 35.655353556688475 38.0 35.0 40.0 27.0 41.0 39 35.49012991481526 38.0 35.0 40.0 27.0 41.0 40 35.506366285853474 38.0 35.0 40.0 27.0 41.0 41 35.56171166644239 38.0 35.0 40.0 26.0 41.0 42 35.22193511242988 38.0 35.0 40.0 26.0 41.0 43 35.20635793941851 38.0 35.0 40.0 26.0 41.0 44 35.32719217411004 38.0 35.0 40.0 26.0 41.0 45 35.31425349656008 38.0 35.0 40.0 26.0 41.0 46 35.212440616818576 38.0 34.0 40.0 26.0 41.0 47 34.93557233548581 38.0 34.0 40.0 24.0 41.0 48 34.987264021584906 38.0 34.0 40.0 25.0 41.0 49 35.06460651669202 38.0 34.0 40.0 26.0 41.0 50 34.9342028388099 38.0 34.0 40.0 25.0 41.0 51 34.38476894853656 37.0 33.0 40.0 23.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 10.0 10 18.0 11 16.0 12 9.0 13 12.0 14 19.0 15 31.0 16 53.0 17 103.0 18 189.0 19 424.0 20 903.0 21 1843.0 22 3495.0 23 5428.0 24 6861.0 25 7759.0 26 7774.0 27 7353.0 28 7792.0 29 9245.0 30 12131.0 31 16144.0 32 21560.0 33 28184.0 34 41595.0 35 54146.0 36 52046.0 37 71121.0 38 108877.0 39 121877.0 40 58.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.422648136445474 18.097115029204005 20.592528748358394 11.887708085992127 2 25.929477734607215 35.99067924650429 22.46877326142908 15.611069757459415 3 24.929608892870952 36.87778604850811 23.768091749463867 14.42451330915706 4 23.0615404793579 24.197507311647364 36.630629372296994 16.11032283669774 5 31.416492214820202 27.0601641692657 23.545804042740563 17.977539573173537 6 18.93107718408318 42.879554130037455 24.510243119727054 13.679125566152312 7 70.68868308586438 5.58206163757054 19.205998531708786 4.523256744856297 8 69.68915491494302 14.521263820589123 9.478313747600401 6.3112675168674635 9 65.10372574636718 6.915617542502943 10.511909000011924 17.468747711117963 10 38.47212546224771 26.397729769689494 19.585505819509198 15.544638948553596 11 30.41440901278708 23.9850990585562 26.159600870073263 19.440891058583457 12 23.096970244107673 20.371263054079787 31.634010530134887 24.897756171677653 13 24.607504637381467 24.562536089814454 35.85219656024678 14.977762712557297 14 18.82495822524132 34.57928006036687 26.940929384050133 19.654832330341677 15 14.717319874565005 25.704294325957243 39.035765325502446 20.542620473975305 16 16.993341588922746 26.135072571400343 29.150349954094608 27.721235885582303 17 17.635676410419755 28.366296073598523 30.537050506151665 23.460977009830057 18 18.799237578716248 24.929608892870952 31.067134294138587 25.20401923427421 19 18.63605625837837 28.58858378032183 27.075664691343725 25.69969526995607 20 22.16012550312821 27.25553888161178 31.916767306503235 18.667568308756774 21 18.502002292714582 34.93221502460495 28.190169262294383 18.375613420386085 22 16.022770437268026 24.30192291641471 32.285373128226794 27.38993351809047 23 18.693799961504197 31.29844977745679 29.12973936979306 20.878010891245953 24 18.93754992956631 28.001948637061236 28.12390878879602 24.936592644576436 25 16.22291454102273 35.91709435048554 26.228416374683388 21.631574733808343 26 15.636960739391936 27.272572422356863 33.068745667093076 24.021721171158127 27 22.557688344118404 29.107084760602103 26.097258110946264 22.23796878433323 28 14.342752313580673 28.677669198418602 33.4097571528096 23.569821335191126 29 18.855448263175017 24.947323775245835 32.535255170957136 23.661972790622013 30 17.171512425116298 34.06708149016227 27.54936745946443 21.212038625256994 31 19.873372658101065 26.422769074584764 22.988466589561504 30.715391677752663 32 22.801608647587965 33.36121156168612 22.795817243734636 21.04136254699128 33 20.09651204186163 26.332831979450738 22.024879189612264 31.54577678907537 34 17.463296978079537 25.196183805531476 26.966479695167756 30.374039521221235 35 17.813336240390953 30.53398446881755 25.089383505059814 26.563295785731682 36 17.83139179358074 27.767907787223823 25.03845321823202 29.362247200963417 37 15.961960696808083 33.05086044931074 26.370816775312267 24.61636207856891 38 15.709523622965982 28.812063834897295 21.92506264084609 33.55334990129063 39 18.345293717859835 28.98904232323869 24.158159832526227 28.507504126375245 40 18.1902884970796 26.322441519596236 26.447808379480037 29.03946160384413 41 16.14762629092947 25.60992851022949 23.332373777204694 34.910071421636346 42 18.31463334451869 27.34905302030228 29.232110949670997 25.10420268550803 43 19.564384228985297 25.478940581899817 25.621511317936147 29.335163871178736 44 19.413126387168976 25.04901401349397 28.989382994053592 26.548476605283465 45 17.947730876869645 23.888518882531592 26.915549408339963 31.2482008322588 46 23.489593358281795 27.907753156740938 24.455395118527893 24.147258366449375 47 15.531523122179886 26.117017018210557 33.25526293825171 25.096196921357844 48 18.928522152971418 26.18276648548657 26.05586660693572 28.832844754606295 49 18.464017496853053 21.918419559955506 33.25781796936347 26.359744973827965 50 20.242830156861878 22.008015984274635 28.444480025618446 29.304673833245044 51 18.008370281922133 22.022664829315406 27.26184129168746 32.707123597075 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1711.0 1 1764.5 2 1818.0 3 2294.5 4 2771.0 5 2033.0 6 1295.0 7 1321.0 8 1347.0 9 1434.0 10 1521.0 11 1613.0 12 1705.0 13 1699.5 14 1694.0 15 1711.5 16 1729.0 17 1707.5 18 1686.0 19 1750.5 20 1815.0 21 1806.0 22 1797.0 23 2047.5 24 2298.0 25 2184.5 26 2855.0 27 3639.0 28 4287.0 29 4935.0 30 6070.5 31 7206.0 32 8906.0 33 10606.0 34 11637.5 35 12669.0 36 14404.0 37 16139.0 38 17428.0 39 18717.0 40 21775.0 41 24833.0 42 29596.0 43 34359.0 44 40545.5 45 46732.0 46 76152.5 47 105573.0 48 88463.0 49 71353.0 50 67652.5 51 63952.0 52 54265.5 53 44579.0 54 37435.0 55 30291.0 56 26216.5 57 22142.0 58 19144.0 59 16146.0 60 12840.5 61 9535.0 62 8141.5 63 6748.0 64 5681.0 65 4614.0 66 3855.5 67 3097.0 68 2355.5 69 1614.0 70 1220.5 71 827.0 72 800.0 73 773.0 74 581.0 75 262.0 76 135.0 77 119.0 78 103.0 79 81.0 80 59.0 81 42.5 82 26.0 83 16.5 84 7.0 85 8.0 86 9.0 87 8.0 88 7.0 89 5.5 90 4.0 91 2.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 587077.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.168517843667043 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75896947571309 22.25198786758543 2 11.189580763910351 6.751461338783658 3 4.380310454265668 3.9644242230094537 4 2.18553960782331 2.637379626266343 5 1.3404072693514006 2.021905031160437 6 0.8618228518233412 1.5599950849987492 7 0.6298305701137129 1.3300738355073773 8 0.4756561612739939 1.1479873111075722 9 0.34971042062753765 0.9495220558336358 >10 4.082601065513248 28.209598321902224 >50 0.6458960441319367 12.823807373880763 >100 0.08600555790469344 4.4332604883575435 >500 0.005695732311569102 1.25832428512567 >1k 0.006265305542726012 4.191321311318496 >5k 0.0 0.0 >10k+ 0.0017087196934707305 6.4689518451626515 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG 13527 2.304127056587126 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC 12827 2.1848922713715577 No Hit GAATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC 11293 1.923597756342013 No Hit GCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC 3990 0.6796382757287375 TruSeq Adapter, Index 16 (95% over 22bp) GAATGACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCT 3591 0.6116744481558637 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACACCACT 2754 0.46910371211953455 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTC 2302 0.3921121079517679 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACACCACTCGTA 2138 0.36417710112983476 No Hit GAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCT 2015 0.34322584601338496 No Hit CCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC 1994 0.3396488024569179 TruSeq Adapter, Index 16 (95% over 22bp) CTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGCT 1658 0.28241610555344526 Illumina Single End Adapter 1 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1502 0.25584378199111873 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1388 0.2364255455417262 No Hit TCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC 1060 0.18055553189786008 TruSeq Adapter, Index 16 (95% over 22bp) GAATGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTT 904 0.1539832083355335 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 890 0.15159851263122215 No Hit GAATGATACGGCGACCACCGATGAATGATACGGCGAACACCACTCGAATGC 829 0.14120805277672263 No Hit ACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTCTGC 750 0.12775155558810855 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCC 731 0.12451518284654313 No Hit AAAACTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCGTCTTC 676 0.1151467354367485 No Hit GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTACACCAC 676 0.1151467354367485 No Hit GAATGATCCGGCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGC 640 0.10901466076851929 No Hit GAATGATCCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATGCCG 625 0.10645962965675712 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACACCACTCGTATG 602 0.10254191528538846 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.703354074508114E-4 0.0 0.0 0.30030132333578047 0.0 2 1.703354074508114E-4 0.0 0.0 1.7047167577677205 0.0 3 1.703354074508114E-4 0.0 0.0 2.1658147057370667 0.0 4 1.703354074508114E-4 0.0 0.0 3.0801751729330222 0.0 5 1.703354074508114E-4 0.0 0.0 6.636267474283612 0.0 6 1.703354074508114E-4 0.0 0.0 7.893853787492953 0.0 7 1.703354074508114E-4 0.0 0.0 9.565014469992862 0.0 8 1.703354074508114E-4 0.0 0.0 11.608017346957896 0.0 9 1.703354074508114E-4 0.0 0.0 12.32104136254699 0.0 10 3.406708149016228E-4 0.0 0.0 16.25698162251289 0.0 11 3.406708149016228E-4 0.0 0.0 18.021997114518197 0.0 12 3.406708149016228E-4 0.0 0.0 21.863060552533994 0.0 13 3.406708149016228E-4 0.0 0.0 22.565012766638787 0.0 14 3.406708149016228E-4 0.0 0.0 22.874341866569463 0.0 15 3.406708149016228E-4 0.0 0.0 23.651582330767514 0.0 16 3.406708149016228E-4 0.0 0.0 24.579228959744633 0.0 17 3.406708149016228E-4 0.0 0.0 25.6954368847698 0.0 18 3.406708149016228E-4 0.0 0.0 27.04142727444611 0.0 19 3.406708149016228E-4 0.0 0.0 28.291859500542518 0.0 20 3.406708149016228E-4 0.0 0.0 29.085281828448398 0.0 21 3.406708149016228E-4 0.0 0.0 29.796261819148086 0.0 22 3.406708149016228E-4 0.0 0.0 30.640273763066855 0.0 23 3.406708149016228E-4 0.0 0.0 31.443745880012333 0.0 24 3.406708149016228E-4 0.0 0.0 32.01658385526941 0.0 25 3.406708149016228E-4 0.0 0.0 32.51447425124813 0.0 26 5.110062223524341E-4 0.0 0.0 33.00640290796608 0.0 27 6.813416298032456E-4 0.0 0.0 33.56357002573768 0.0 28 8.51677037254057E-4 0.0 0.0 34.10319259654185 0.0 29 8.51677037254057E-4 0.0 0.0 34.6068743963739 0.0 30 8.51677037254057E-4 0.0 0.0 35.18669612333646 0.0 31 0.0011923478521556799 0.0 0.0 35.71814259458299 0.0 32 0.0011923478521556799 0.0 0.0 36.21381863026485 0.0 33 0.0011923478521556799 0.0 0.0 36.6960381687581 0.0 34 0.0011923478521556799 0.0 0.0 37.16957060147136 0.0 35 0.0011923478521556799 0.0 0.0 37.66984569315439 0.0 36 0.0011923478521556799 0.0 0.0 38.14831785268372 0.0 37 0.0011923478521556799 0.0 0.0 38.57994777516407 0.0 38 0.0011923478521556799 0.0 0.0 39.05007349972831 0.0 39 0.0011923478521556799 0.0 0.0 39.547282554077235 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTACGC 35 1.2105738E-7 45.000004 8 TTTCGCA 35 1.2105738E-7 45.000004 13 CGCATAC 35 1.2105738E-7 45.000004 16 ACGGGTT 35 1.2105738E-7 45.000004 5 ATTTTCG 35 1.2105738E-7 45.000004 11 AGCGCAG 35 1.2105738E-7 45.000004 1 CGTCAGG 35 1.2105738E-7 45.000004 2 AACCGAT 25 3.8884657E-5 45.0 16 TCCATAC 20 7.030464E-4 45.0 18 GTCGAGC 20 7.030464E-4 45.0 16 AGTCACG 20 7.030464E-4 45.0 2 GAATGCG 25 3.8884657E-5 45.0 1 TCCGAGC 20 7.030464E-4 45.0 23 ACGCAAT 20 7.030464E-4 45.0 27 GACGTAG 20 7.030464E-4 45.0 1 GGCCGTA 25 3.8884657E-5 45.0 42 GACCGCA 20 7.030464E-4 45.0 21 TTACTCG 20 7.030464E-4 45.0 26 CGCGACT 75 0.0 45.0 24 CCCGTTG 20 7.030464E-4 45.0 26 >>END_MODULE