##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552617_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	831485
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.735700583895078	31.0	31.0	34.0	30.0	34.0
2	31.874464361954814	33.0	31.0	34.0	30.0	34.0
3	31.9277822209661	33.0	31.0	34.0	30.0	34.0
4	35.57941875078925	37.0	35.0	37.0	33.0	37.0
5	35.3745611766899	37.0	35.0	37.0	33.0	37.0
6	35.37614027913913	37.0	35.0	37.0	33.0	37.0
7	35.71822943288213	37.0	35.0	37.0	35.0	37.0
8	35.46525553678058	37.0	35.0	37.0	33.0	37.0
9	37.21928717896294	39.0	37.0	39.0	34.0	39.0
10	36.77602843106009	39.0	37.0	39.0	32.0	39.0
11	36.71504116129575	39.0	35.0	39.0	32.0	39.0
12	36.58996253690686	39.0	35.0	39.0	32.0	39.0
13	36.38853015989465	39.0	35.0	39.0	32.0	39.0
14	37.517269704203926	39.0	36.0	41.0	32.0	41.0
15	37.64477771697625	40.0	36.0	41.0	33.0	41.0
16	37.732526744318896	40.0	36.0	41.0	33.0	41.0
17	37.63038539480568	40.0	36.0	41.0	33.0	41.0
18	37.63449009903967	39.0	36.0	41.0	33.0	41.0
19	37.59421035857532	39.0	36.0	41.0	33.0	41.0
20	37.449624467067956	39.0	35.0	41.0	32.0	41.0
21	37.41415659933733	39.0	35.0	41.0	32.0	41.0
22	37.44335255596914	39.0	35.0	41.0	32.0	41.0
23	37.258271646511965	39.0	35.0	41.0	32.0	41.0
24	37.28585362333656	39.0	35.0	41.0	32.0	41.0
25	37.17108426489955	39.0	35.0	41.0	32.0	41.0
26	37.11839299566439	39.0	35.0	41.0	32.0	41.0
27	36.84322266787735	39.0	35.0	41.0	31.0	41.0
28	36.89998015598598	39.0	35.0	41.0	31.0	41.0
29	36.864309037445054	39.0	35.0	41.0	31.0	41.0
30	36.75111036278466	39.0	35.0	41.0	31.0	41.0
31	36.73316536077019	39.0	35.0	41.0	31.0	41.0
32	36.55236594767194	39.0	35.0	40.0	30.0	41.0
33	36.43852865656025	39.0	35.0	40.0	30.0	41.0
34	36.301174404829915	39.0	35.0	40.0	30.0	41.0
35	36.17909763856233	38.0	35.0	40.0	30.0	41.0
36	36.15807140237046	38.0	35.0	40.0	30.0	41.0
37	36.06537219552968	38.0	35.0	40.0	30.0	41.0
38	35.948323782148805	38.0	35.0	40.0	29.0	41.0
39	35.911593113525804	38.0	35.0	40.0	29.0	41.0
40	35.8295002315135	38.0	35.0	40.0	29.0	41.0
41	35.75403164218236	38.0	35.0	40.0	29.0	41.0
42	35.693272879246166	38.0	35.0	40.0	29.0	41.0
43	35.642463784674405	38.0	35.0	40.0	29.0	41.0
44	35.59717733933865	38.0	35.0	40.0	29.0	41.0
45	35.51143917208368	38.0	34.0	40.0	29.0	41.0
46	35.36157838084872	37.0	34.0	40.0	28.0	41.0
47	35.25020776081348	37.0	34.0	40.0	28.0	41.0
48	35.202941724745486	37.0	34.0	40.0	28.0	41.0
49	35.170777584682824	37.0	34.0	40.0	28.0	41.0
50	35.07667366218272	37.0	34.0	40.0	28.0	41.0
51	34.39168114878801	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	26.0
10	41.0
11	35.0
12	37.0
13	40.0
14	52.0
15	67.0
16	124.0
17	194.0
18	340.0
19	627.0
20	1194.0
21	2053.0
22	3124.0
23	4630.0
24	5704.0
25	6804.0
26	8056.0
27	9111.0
28	10905.0
29	13091.0
30	16489.0
31	21654.0
32	27681.0
33	37611.0
34	60299.0
35	75670.0
36	77212.0
37	105533.0
38	155835.0
39	187143.0
40	97.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.867814813255805	18.929625910268978	21.896726940353705	13.305832336121517
2	29.275573221405075	27.770795624695577	24.81848740506443	18.135143748834917
3	31.65601303691588	27.520039447494543	24.658171825108088	16.16577569048149
4	27.60747337594785	25.785792888626975	27.319193972230405	19.287539763194765
5	26.952139846178824	29.60101505138397	23.084240846196867	20.362604256240342
6	24.146677330318646	37.70266450988292	22.672567755281214	15.47809040451722
7	79.91196473778841	5.945507134825042	9.719477801764313	4.4230503256222296
8	81.17332242914786	7.402178030872475	6.525313144554621	4.899186395425053
9	76.21688905993493	6.968135324148962	8.456436375881706	8.358539240034396
10	42.51309404258646	25.9386519299807	15.310077752454946	16.2381762749779
11	32.819233058924695	25.21224074998346	21.6268483496395	20.34167784145234
12	27.173069868969375	21.761667378244947	27.939409610516126	23.125853142269552
13	25.679717613667112	23.304449268477484	29.855018430879692	21.160814686975712
14	20.695863425076823	28.992224754505493	26.87204218957648	23.439869630841205
15	20.218765221260757	24.8421799551405	32.1251736351227	22.81388118847604
16	22.378756080987632	23.23156761697445	29.594520646794592	24.795155655243327
17	22.020721961310187	24.145234129298785	28.09491452040626	25.739129388984765
18	23.375647185457343	23.650817513244377	27.67530382388137	25.29823147741691
19	24.525036531025815	27.00686121818193	24.328520658821265	24.13958159197099
20	27.703325977017023	24.97375178145126	25.367144326115326	21.955777915416395
21	24.5537802846714	28.316325610203428	24.72191320348533	22.40798090163984
22	23.023145336356038	23.69026500778727	25.864808144464423	27.42178151139227
23	23.28881459076231	27.73315213142751	24.84939596023981	24.12863731757037
24	23.427602422172377	26.007925578934078	26.84029176713952	23.72418023175403
25	23.36109490850707	27.385942019399028	25.062749177676086	24.19021389441782
26	21.65198410073543	26.54672062634924	26.810104812474066	24.99119046044126
27	23.552319043638793	25.25866371612236	26.414667733031866	24.774349507206985
28	21.165745623793576	27.527255452593852	27.82275086141061	23.484248062201964
29	23.392364263937413	25.94755166960318	26.305104722274002	24.354979344185406
30	23.10973739754776	26.894291538632686	26.469990438793246	23.525980625026307
31	24.757632428726918	26.98208626734096	22.764692087049074	25.495589216883047
32	26.37293517020752	27.94975255115847	23.449009903967	22.22830237466701
33	24.8317167477465	25.795775029014358	23.231928417229415	26.14057980600973
34	23.48436832895362	27.351786261928957	24.475486629343884	24.688358779773537
35	24.708804127554917	25.158601778745254	24.48631063699285	25.646283456706975
36	24.142467994010715	29.452846413344798	22.897707114379696	23.50697847826479
37	24.602247785588435	26.735419159696207	25.807200370421597	22.855132684293764
38	23.278952717126586	28.37742112004426	23.730794903095067	24.61283125973409
39	26.205163051648555	25.293060007095736	23.49531260335424	25.006464337901463
40	24.623895800886366	24.251068870755336	26.15717661773814	24.967858710620156
41	20.95960841145661	25.184459130351115	25.51940203371077	28.3365304244815
42	22.567093814079627	25.257220515102496	25.48584760999898	26.6898380608189
43	22.84154254135673	24.72395773826347	26.028851993722075	26.405647726657726
44	23.374805318195758	24.50170478120471	26.711365809365173	25.412124091234357
45	24.204766171368096	24.891008256312503	25.70930323457429	25.19492233774512
46	24.67188223479678	26.037390933089593	25.052646770537052	24.23808006157658
47	22.422653445341766	26.320258332982554	27.63296992729875	23.624118294376927
48	23.42279175210617	25.067800381245604	25.437139575578634	26.072268291069594
49	22.68507549745335	24.154374402424576	27.92557893407578	25.23497116604629
50	22.88928844176383	24.263576612927473	27.692020902361435	25.155114042947258
51	22.594033566450385	24.30530917575182	25.378449400770908	27.722207857026888
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1757.0
1	1964.0
2	2171.0
3	2774.5
4	3378.0
5	2471.0
6	1564.0
7	1606.0
8	1648.0
9	1716.0
10	1784.0
11	1803.5
12	1823.0
13	1981.5
14	2140.0
15	2102.0
16	2064.0
17	1960.0
18	1856.0
19	1835.5
20	1815.0
21	2006.5
22	2198.0
23	2410.0
24	2622.0
25	3249.5
26	4526.5
27	5176.0
28	6710.5
29	8245.0
30	8865.5
31	9486.0
32	11291.0
33	13096.0
34	15100.0
35	17104.0
36	17756.5
37	18409.0
38	20743.0
39	23077.0
40	25758.5
41	28440.0
42	31208.0
43	33976.0
44	39881.5
45	45787.0
46	59613.5
47	73440.0
48	73697.0
49	73954.0
50	72670.5
51	71387.0
52	67246.0
53	63105.0
54	56303.0
55	49501.0
56	47856.5
57	46212.0
58	43355.5
59	40499.0
60	40372.5
61	40246.0
62	37592.0
63	34938.0
64	30665.5
65	26393.0
66	23614.0
67	20835.0
68	18574.5
69	16314.0
70	14134.5
71	11955.0
72	11156.0
73	10357.0
74	8807.5
75	5933.0
76	4608.0
77	3854.0
78	3100.0
79	2660.0
80	2220.0
81	1608.0
82	996.0
83	723.0
84	450.0
85	301.0
86	152.0
87	90.0
88	28.0
89	22.0
90	16.0
91	22.0
92	28.0
93	14.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	831485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.970757100396618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.60475481119684	18.87907968025439
2	9.489924206984655	4.739411845475757
3	3.40023241435134	2.5471913311098735
4	1.6700783462108497	1.6681248288745287
5	1.0154026278223889	1.267768618922865
6	0.7015733875737322	1.0511291189523648
7	0.49059885178996304	0.8575437333246455
8	0.4021928264016815	0.8034447500478699
9	0.3299937367309708	0.7416174100105171
>10	5.192946929337175	33.72392482684054
>50	1.5826885345644564	25.78598081398949
>100	0.10940243326457069	4.429015421307186
>500	0.00632102947750853	1.137304332786458
>1k	0.0038898642938514027	2.368463288103495
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	4147	0.4987462191139948	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	4115	0.4948976830610294	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	3676	0.4421005790844092	No Hit
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	2359	0.2837092671545488	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1549	0.18629319831386015	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1427	0.17162065461192927	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1188	0.14287690096634337	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	1046	0.12579902223130907	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	941	0.11317101330751607	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	927	0.11148727878434368	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGAGCCT	874	0.10511314094661961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11822221687703326	0.0
2	0.0	0.0	0.0	0.6639927358882001	0.0
3	0.0	0.0	0.0	0.8685664804536462	0.0
4	0.0	0.0	0.0	1.223233131084746	0.0
5	0.0	0.0	0.0	2.4488715972025954	0.0
6	0.0	0.0	0.0	3.2034251970871392	0.0
7	0.0	0.0	0.0	3.9125179648460287	0.0
8	0.0	0.0	0.0	5.016085678033879	0.0
9	0.0	0.0	0.0	5.526858572313391	0.0
10	0.0	0.0	0.0	7.005418017162065	0.0
11	0.0	0.0	0.0	8.0551062256084	0.0
12	0.0	0.0	0.0	9.453086946848108	0.0
13	0.0	0.0	0.0	9.82687601099238	0.0
14	0.0	0.0	0.0	9.993445462034794	0.0
15	0.0	0.0	0.0	10.304816082070031	0.0
16	0.0	0.0	0.0	10.78480068792582	0.0
17	0.0	0.0	0.0	11.370138968231538	0.0
18	0.0	0.0	0.0	12.083922139304978	0.0
19	0.0	0.0	0.0	12.556450206558146	0.0
20	0.0	0.0	0.0	12.928074469172625	0.0
21	0.0	0.0	0.0	13.345039297161103	0.0
22	0.0	0.0	0.0	13.763206792666134	0.0
23	0.0	0.0	0.0	14.20013590142937	0.0
24	1.2026675165517117E-4	0.0	0.0	14.54734601345785	0.0
25	1.2026675165517117E-4	0.0	0.0	14.850538494380537	0.0
26	1.2026675165517117E-4	0.0	0.0	15.129677624972189	0.0
27	1.2026675165517117E-4	0.0	0.0	15.439845577490875	0.0
28	1.2026675165517117E-4	0.0	0.0	15.734980186052665	0.0
29	1.2026675165517117E-4	0.0	0.0	16.04490760506804	0.0
30	2.4053350331034235E-4	0.0	0.0	16.414487332904383	0.0
31	2.4053350331034235E-4	0.0	0.0	16.71960408185355	0.0
32	2.4053350331034235E-4	0.0	0.0	17.04660937960396	0.0
33	2.4053350331034235E-4	0.0	0.0	17.350643727788235	0.0
34	2.4053350331034235E-4	0.0	0.0	17.65732394450892	0.0
35	2.4053350331034235E-4	0.0	0.0	18.000204453477814	0.0
36	3.6080025496551353E-4	0.0	0.0	18.314100675297812	0.0
37	3.6080025496551353E-4	0.0	0.0	18.63112383266084	0.0
38	3.6080025496551353E-4	0.0	0.0	18.95596432888146	0.0
39	3.6080025496551353E-4	0.0	0.0	19.278279223317316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACTCGA	30	2.1647738E-6	45.000004	45
AAGTACG	45	3.8380676E-10	45.0	1
ATCTACG	25	3.8898717E-5	45.0	1
GACGTAT	25	3.8898717E-5	45.0	19
CGTTGCC	20	7.0321606E-4	45.0	44
TATATCG	20	7.0321606E-4	45.0	28
GTTAGCG	25	3.8898717E-5	45.0	1
TAGCCGT	50	2.1827873E-11	45.0	44
GTCGTAT	20	7.0321606E-4	45.0	9
GTATCAC	20	7.0321606E-4	45.0	12
TATCGGC	20	7.0321606E-4	45.0	30
TCGAATA	20	7.0321606E-4	45.0	44
CCGTTCA	25	3.8898717E-5	45.0	38
GTTATCG	20	7.0321606E-4	45.0	1
GTACGAC	20	7.0321606E-4	45.0	1
CTCGAAT	135	0.0	41.666668	43
ACGCATT	55	6.002665E-11	40.909092	17
TCGTTTA	50	1.0804797E-9	40.5	38
CGTCATA	50	1.0804797E-9	40.5	38
TATAGCG	45	1.9272193E-8	40.0	1
>>END_MODULE