FastQCFastQC Report
Sat 18 Jun 2016
SRR3552616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939791
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC47810.24646985164896631No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.22476648257466914No Hit
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC38960.20084637984195206No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT33010.17017297224288597Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23030.11872413058932638No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC21060.10856839731703055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA207.0344895E-445.04
TACGCGG2800.041.7857132
TAATGCG1250.041.3999981
CGTAAGG3050.040.5737722
TAGTCCG501.0822987E-940.51
GCGTAAG1900.039.0789451
GTTAGCG1750.038.5714261
CGTTTTT32450.038.3436051
TACGGGA6750.037.6666684
ATTAGCG1500.037.51
CGACATA301.14021146E-437.49999617
CGCACTA301.14021146E-437.49999639
CGCGTAA301.14021146E-437.49999631
TATCGTC301.14021146E-437.49999639
AATCGTC301.14021146E-437.49999624
TCGGCGT2350.037.3404274
ACGGGAC6650.037.2180445
AATACGG2200.036.818182
AATGCGG2400.036.56252
CGAACAC250.00210746536.015
Produced by FastQC (version 0.11.5)