##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552616_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1939791 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.67081917588029 31.0 31.0 34.0 30.0 34.0 2 31.819566644035362 33.0 31.0 34.0 30.0 34.0 3 31.86935138888674 33.0 31.0 34.0 30.0 34.0 4 35.511009175730784 37.0 35.0 37.0 33.0 37.0 5 35.33160634315759 37.0 35.0 37.0 33.0 37.0 6 35.33489896591952 37.0 35.0 37.0 32.0 37.0 7 35.77747808913435 37.0 35.0 37.0 35.0 37.0 8 35.621482932955146 37.0 35.0 37.0 35.0 37.0 9 37.32595985856208 39.0 37.0 39.0 34.0 39.0 10 36.750117409555976 39.0 37.0 39.0 32.0 39.0 11 36.70824640386516 39.0 37.0 39.0 32.0 39.0 12 36.67594653238416 39.0 35.0 39.0 32.0 39.0 13 36.54787500302868 39.0 35.0 39.0 32.0 39.0 14 37.68829064574483 40.0 37.0 41.0 33.0 41.0 15 37.800699147485474 40.0 37.0 41.0 33.0 41.0 16 37.84500031188927 40.0 37.0 41.0 33.0 41.0 17 37.81606059621887 40.0 37.0 41.0 33.0 41.0 18 37.791948720248726 40.0 36.0 41.0 33.0 41.0 19 37.75418382702054 40.0 36.0 41.0 33.0 41.0 20 37.68325195858729 39.0 36.0 41.0 33.0 41.0 21 37.60606941675675 39.0 36.0 41.0 32.0 41.0 22 37.637038732523244 39.0 36.0 41.0 33.0 41.0 23 37.54502882011516 39.0 36.0 41.0 32.0 41.0 24 37.54272444814931 39.0 36.0 41.0 32.0 41.0 25 37.38961104572606 39.0 36.0 41.0 32.0 41.0 26 37.39246032175631 39.0 36.0 41.0 32.0 41.0 27 37.18367958197558 39.0 35.0 41.0 32.0 41.0 28 37.20573711291577 39.0 35.0 41.0 32.0 41.0 29 37.203842578917005 39.0 35.0 41.0 32.0 41.0 30 37.08513082079461 39.0 35.0 41.0 31.0 41.0 31 37.099943241307955 39.0 35.0 41.0 31.0 41.0 32 37.00375298163565 39.0 35.0 41.0 31.0 41.0 33 36.92984605042502 39.0 35.0 41.0 31.0 41.0 34 36.854712698429886 39.0 35.0 41.0 31.0 41.0 35 36.73680257306071 39.0 35.0 41.0 31.0 41.0 36 36.69078318231191 39.0 35.0 41.0 31.0 41.0 37 36.64871318611129 39.0 35.0 41.0 30.0 41.0 38 36.57302049550699 39.0 35.0 41.0 30.0 41.0 39 36.54618255265645 39.0 35.0 40.0 30.0 41.0 40 36.4414697253467 39.0 35.0 40.0 30.0 41.0 41 36.36871961979409 39.0 35.0 40.0 30.0 41.0 42 36.307005754743685 39.0 35.0 40.0 30.0 41.0 43 36.24795506320011 38.0 35.0 40.0 30.0 41.0 44 36.197890391284425 38.0 35.0 40.0 30.0 41.0 45 36.190282355160946 38.0 35.0 40.0 30.0 41.0 46 36.11971083482705 38.0 35.0 40.0 30.0 41.0 47 36.04382173131023 38.0 35.0 40.0 30.0 41.0 48 35.98289557998774 38.0 35.0 40.0 30.0 41.0 49 35.93511878341533 38.0 35.0 40.0 30.0 41.0 50 35.846899485563135 38.0 35.0 40.0 29.0 41.0 51 35.181213336900726 37.0 34.0 40.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 13.0 9 48.0 10 93.0 11 81.0 12 64.0 13 74.0 14 77.0 15 103.0 16 201.0 17 356.0 18 666.0 19 1120.0 20 1702.0 21 2775.0 22 4210.0 23 6262.0 24 8752.0 25 11905.0 26 15544.0 27 19079.0 28 23407.0 29 28544.0 30 36887.0 31 46846.0 32 60820.0 33 82463.0 34 127262.0 35 160927.0 36 175387.0 37 246700.0 38 385015.0 39 492190.0 40 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68253641758314 19.034267093722985 23.225491818448482 15.057704670245403 2 30.482201433040984 24.847058265555415 25.87546802722561 18.79527227417799 3 31.50875532467158 24.60182566059952 27.28262993281235 16.606789081916556 4 28.252322028507194 26.23298076957775 26.300256058513522 19.21444114340153 5 24.556769260193494 30.582005999615426 24.8770614978624 19.984163242328684 6 23.944950770469603 36.335821745744774 24.553676143460816 15.165551340324809 7 82.06574832030873 5.799078354317553 7.826203957024235 4.3089693683494765 8 83.06379398605314 4.928675305741701 6.925230604740408 5.082300103464755 9 77.39864758626058 7.22577844726571 9.449007650824239 5.9265663156494695 10 37.555746985113345 27.38800210950561 18.45657599194965 16.599674913431393 11 28.562149221230538 26.43707492198902 24.733850193139364 20.266925663641082 12 24.79669201475829 22.741779913403043 30.90987637327939 21.551651698559276 13 23.75766255230589 24.37819332082683 31.374256298745586 20.489887828121688 14 21.3607032922619 27.50146794164938 29.175772029048492 21.96205673704023 15 20.482515899908805 26.481151835429696 32.4265861631485 20.609746101513 16 22.012113676164084 25.431657328031733 30.769191113888045 21.787037881916145 17 22.283586221402203 25.346957481501875 28.476985407190774 23.892470889905148 18 23.182652151700882 24.93397484574369 29.58050635351953 22.302866649035902 19 23.30519112626051 28.132102891497073 26.490637393409912 22.072068588832508 20 23.961241185261713 26.676585260989455 27.195610248732983 22.16656330501585 21 23.37782781753292 28.06529157007121 27.15905991934183 21.397820693054044 22 21.898647844020307 25.139048485120302 27.10554899986648 25.856754670992903 23 21.007160049716695 27.821863283209375 27.326861502089656 23.84411516498427 24 21.62207165617327 26.38366710640476 28.798463339607206 23.195797897814767 25 22.13573524158015 27.31979888555004 26.897949315158183 23.64651655771163 26 20.71800518715676 27.61086117009513 27.22695383162413 24.44417981112398 27 21.86848995587669 26.671223858652816 28.165508552210007 23.29477763326049 28 20.84307020704808 26.51723819731095 29.775269603787212 22.86442199185376 29 21.842404671431098 25.4444422105268 27.96718821769974 24.745964900342358 30 21.68197501689615 25.689417055754976 28.590966758789992 24.03764116855888 31 22.37524558058059 25.751640254027368 27.575908951015858 24.297205214376188 32 22.38493734634298 25.892222409527626 28.197522310393232 23.525317933736158 33 22.038456720337397 25.424904023165386 27.296342750327224 25.240296506169997 34 20.78564133971134 24.9565030459467 29.167523717761345 25.090331896580608 35 21.71744275543087 23.86721043658827 29.44291421086086 24.972432597119997 36 23.157391698384 25.160442542521334 28.291449955175587 23.390715803919083 37 21.930042978857 25.47573424147241 31.181142710735333 21.413080068935262 38 22.575370233184916 25.674724751274752 29.08266921539485 22.66723580014548 39 23.293076419057517 24.064396628296553 28.34444535519548 24.298081597450448 40 23.570168126360006 23.519286356107436 29.66608258312365 23.24446293440891 41 21.879728280005423 24.431652688356632 28.721444732963498 24.967174298674443 42 23.114088064126495 24.778236418253307 28.104935016195043 24.002740501425155 43 22.535314371496725 24.099245743484737 29.594167619088864 23.77127226592968 44 21.416379393450118 24.594041316822278 29.422448088479637 24.567131201247967 45 21.557837932024633 24.04970432381633 29.030653302340305 25.36180444181873 46 22.84586329145769 24.878556504283196 28.138340676907976 24.13723952735114 47 21.59933724818808 24.81999349414447 29.981064970401448 23.599604287266 48 21.857303183693503 24.339529361668344 29.33475822910819 24.468409225529967 49 22.42473544830345 23.592593222671926 29.741348423618835 24.241322905405788 50 21.203057442786363 23.332977624909077 30.346516712367468 25.117448219937096 51 21.284818828420175 22.79044494999719 28.99714453773628 26.92759168384635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2679.0 1 2955.5 2 3232.0 3 5788.5 4 8345.0 5 6038.5 6 3732.0 7 3635.5 8 3539.0 9 3747.5 10 3956.0 11 3997.0 12 4038.0 13 4079.0 14 4120.0 15 4433.5 16 4747.0 17 4479.0 18 4211.0 19 4195.0 20 4179.0 21 4926.5 22 5674.0 23 6653.0 24 7632.0 25 9872.5 26 14412.5 27 16712.0 28 19926.0 29 23140.0 30 25816.5 31 28493.0 32 32464.0 33 36435.0 34 43699.0 35 50963.0 36 55463.5 37 59964.0 38 64382.0 39 68800.0 40 77959.0 41 87118.0 42 95841.5 43 104565.0 44 118129.5 45 131694.0 46 150356.5 47 169019.0 48 181353.0 49 193687.0 50 189453.0 51 185219.0 52 165533.0 53 145847.0 54 129410.0 55 112973.0 56 103366.5 57 93760.0 58 87273.5 59 80787.0 60 75577.0 61 70367.0 62 63406.0 63 56445.0 64 49380.0 65 42315.0 66 37435.0 67 32555.0 68 27821.0 69 23087.0 70 20119.5 71 17152.0 72 15154.0 73 13156.0 74 11366.0 75 7699.5 76 5823.0 77 4903.0 78 3983.0 79 3007.0 80 2031.0 81 1567.5 82 1104.0 83 725.0 84 346.0 85 324.5 86 303.0 87 224.0 88 145.0 89 82.0 90 19.0 91 13.0 92 7.0 93 3.5 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 1.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1939791.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 26.04410949197681 #Duplication Level Percentage of deduplicated Percentage of total 1 73.5688376348938 19.160348625606385 2 10.444917367027957 5.440571430830525 3 4.212312610483332 3.2911779252558766 4 2.1993165216630204 2.2911696119082117 5 1.3007505778456452 1.6938445235582047 6 0.8084296814734491 1.2632898684515066 7 0.5839470460344949 1.0645866563107165 8 0.4335769133496256 0.9033699683576794 9 0.3550665753684075 0.8322653489252438 >10 4.417371896119116 28.44584448066478 >50 1.4921903897272293 26.73546615757948 >100 0.17609523956520734 6.402902698187075 >500 0.00399308136042791 0.7263097971589116 >1k 0.0031944650883423274 1.7488529072054462 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 4781 0.24646985164896631 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4360 0.22476648257466914 No Hit CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC 3896 0.20084637984195206 No Hit CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT 3301 0.17017297224288597 Illumina Single End Adapter 2 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2303 0.11872413058932638 No Hit GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC 2106 0.10856839731703055 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.0620778217859552E-4 0.0 0.0 0.1801224977330032 0.0 2 2.0620778217859552E-4 0.0 0.0 0.826893206536168 0.0 3 2.577597277232444E-4 0.0 0.0 1.1238324128733457 0.0 4 2.577597277232444E-4 0.0 0.0 1.4946971091215497 0.0 5 2.577597277232444E-4 0.0 0.0 2.571307939875997 0.0 6 2.577597277232444E-4 0.0 0.0 3.577498813016454 0.0 7 2.577597277232444E-4 0.0 0.0 4.460635192141834 0.0 8 2.577597277232444E-4 0.0 0.0 5.866456747144409 0.0 9 2.577597277232444E-4 0.0 0.0 6.519259033576297 0.0 10 2.577597277232444E-4 0.0 0.0 7.823574807801459 0.0 11 2.577597277232444E-4 0.0 0.0 9.152738619779141 0.0 12 2.577597277232444E-4 0.0 0.0 10.320699498038707 0.0 13 2.577597277232444E-4 0.0 0.0 10.790234618059369 0.0 14 2.577597277232444E-4 0.0 0.0 10.984946316381507 0.0 15 2.577597277232444E-4 0.0 0.0 11.27152358166421 0.0 16 2.577597277232444E-4 0.0 0.0 11.868185799397976 0.0 17 2.577597277232444E-4 0.0 0.0 12.59223287457257 0.0 18 3.093116732678933E-4 0.0 0.0 13.491041045143524 0.0 19 3.608636188125422E-4 0.0 0.0 13.973103287931535 0.0 20 4.1241556435719104E-4 2.0620778217859552E-4 0.0 14.446968771377948 0.0 21 4.1241556435719104E-4 2.0620778217859552E-4 0.0 15.01187499065621 0.0 22 4.1241556435719104E-4 2.0620778217859552E-4 0.0 15.61889914944445 0.0 23 4.6396750990183996E-4 2.0620778217859552E-4 0.0 16.2373678401436 0.0 24 4.6396750990183996E-4 2.0620778217859552E-4 0.0 16.71824438818409 0.0 25 5.155194554464888E-4 2.0620778217859552E-4 0.0 17.148703133481906 0.0 26 5.155194554464888E-4 2.0620778217859552E-4 0.0 17.54142585464104 0.0 27 5.155194554464888E-4 2.0620778217859552E-4 0.0 17.965079743126967 0.0 28 5.155194554464888E-4 2.0620778217859552E-4 0.0 18.410385448741643 0.0 29 5.155194554464888E-4 2.0620778217859552E-4 0.0 18.85445390766325 0.0 30 5.670714009911377E-4 2.0620778217859552E-4 0.0 19.3770875315949 0.0 31 5.670714009911377E-4 2.0620778217859552E-4 0.0 19.8448183335215 0.0 32 5.670714009911377E-4 2.0620778217859552E-4 0.0 20.305950486418382 0.0 33 6.186233465357866E-4 2.0620778217859552E-4 0.0 20.762752275889515 0.0 34 7.217272376250843E-4 2.0620778217859552E-4 0.0 21.185787541028905 0.0 35 7.217272376250843E-4 2.0620778217859552E-4 0.0 21.64784762894559 0.0 36 7.217272376250843E-4 2.0620778217859552E-4 0.0 22.07031582268399 0.0 37 7.217272376250843E-4 2.0620778217859552E-4 0.0 22.515827736080844 0.0 38 7.217272376250843E-4 2.0620778217859552E-4 0.0 22.96298931173513 0.0 39 7.217272376250843E-4 2.0620778217859552E-4 0.0 23.40288206306762 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGGTA 20 7.0344895E-4 45.0 4 TACGCGG 280 0.0 41.785713 2 TAATGCG 125 0.0 41.399998 1 CGTAAGG 305 0.0 40.573772 2 TAGTCCG 50 1.0822987E-9 40.5 1 GCGTAAG 190 0.0 39.078945 1 GTTAGCG 175 0.0 38.571426 1 CGTTTTT 3245 0.0 38.343605 1 TACGGGA 675 0.0 37.666668 4 ATTAGCG 150 0.0 37.5 1 CGACATA 30 1.14021146E-4 37.499996 17 CGCACTA 30 1.14021146E-4 37.499996 39 CGCGTAA 30 1.14021146E-4 37.499996 31 TATCGTC 30 1.14021146E-4 37.499996 39 AATCGTC 30 1.14021146E-4 37.499996 24 TCGGCGT 235 0.0 37.340427 4 ACGGGAC 665 0.0 37.218044 5 AATACGG 220 0.0 36.81818 2 AATGCGG 240 0.0 36.5625 2 CGAACAC 25 0.002107465 36.0 15 >>END_MODULE