FastQCFastQC Report
Sat 18 Jun 2016
SRR3552612_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552612_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1942457
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187000.962698273372332No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT23790.12247375360175283No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG253.8918028E-545.0000041
CGTTTTT213150.043.5010531
GTAAGCG700.041.7857131
CGGCGAA1100.038.86363617
GCTATCG356.2505187E-638.5714261
CTAACGC3000.037.525
TTTACGG2650.037.358492
GTTTTTT251450.037.179362
GCGAAAG6850.036.7883221
TAACGCC2600.036.34615726
TAGCCCG250.002107465436.0000041
CGTTGAT751.8189894E-1236.025
CGAAAGG7350.035.8163262
AACGCCT2550.035.29411727
TATTGCG2050.035.1219521
TCTAACG3350.034.9253724
TTGCGCG2200.034.7727281
ACGTAGG1950.034.6153832
CTACGCG850.034.4117621
TCGCACG401.5611202E-533.751
Produced by FastQC (version 0.11.5)