##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1942457
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.704698224980014	31.0	31.0	34.0	30.0	34.0
2	31.877196251963365	33.0	31.0	34.0	30.0	34.0
3	31.8261953803868	33.0	31.0	34.0	30.0	34.0
4	35.56313627534612	37.0	35.0	37.0	33.0	37.0
5	35.485275092318645	37.0	35.0	37.0	33.0	37.0
6	35.42591830861635	37.0	35.0	37.0	33.0	37.0
7	35.8365539108459	37.0	35.0	37.0	35.0	37.0
8	35.721005921881414	37.0	35.0	37.0	35.0	37.0
9	37.53711562212188	39.0	37.0	39.0	35.0	39.0
10	36.935666014743184	39.0	37.0	39.0	33.0	39.0
11	36.85833096948864	39.0	37.0	39.0	32.0	39.0
12	36.8667074740908	39.0	37.0	39.0	33.0	39.0
13	36.80321108781301	39.0	37.0	39.0	33.0	39.0
14	37.981220691114395	40.0	37.0	41.0	33.0	41.0
15	38.094922564566424	40.0	37.0	41.0	33.0	41.0
16	38.108364303559874	40.0	37.0	41.0	33.0	41.0
17	38.04702189031726	40.0	37.0	41.0	33.0	41.0
18	37.89474670481766	39.0	37.0	41.0	33.0	41.0
19	37.67836250686631	39.0	37.0	41.0	33.0	41.0
20	37.46779619831996	39.0	35.0	41.0	33.0	41.0
21	37.312122224584634	39.0	35.0	41.0	32.0	41.0
22	37.375275231317865	39.0	35.0	41.0	33.0	41.0
23	33.32803042744318	38.0	34.0	40.0	0.0	41.0
24	34.957920818839234	38.0	34.0	40.0	18.0	41.0
25	36.02570610314668	38.0	34.0	40.0	27.0	41.0
26	36.65221881359536	38.0	35.0	40.0	31.0	41.0
27	30.482118780492954	38.0	31.0	40.0	0.0	41.0
28	32.997828008547934	38.0	31.0	40.0	16.0	41.0
29	34.89390035403615	38.0	32.0	40.0	25.0	41.0
30	35.78567659412795	38.0	34.0	40.0	30.0	41.0
31	36.0248942447632	38.0	35.0	40.0	30.0	41.0
32	34.27868313172441	38.0	34.0	40.0	21.0	41.0
33	34.60148821827201	38.0	34.0	40.0	16.0	41.0
34	34.79000358823902	38.0	34.0	40.0	22.0	41.0
35	34.83925461413045	38.0	34.0	40.0	22.0	41.0
36	34.79663539527516	38.0	34.0	40.0	21.0	41.0
37	34.793713322868925	38.0	34.0	40.0	21.0	41.0
38	34.7170547404653	38.0	34.0	40.0	21.0	41.0
39	34.64948052904131	38.0	34.0	40.0	20.0	41.0
40	34.60212709985343	38.0	34.0	40.0	20.0	41.0
41	34.51704104646847	38.0	34.0	40.0	18.0	41.0
42	34.439689012420864	38.0	34.0	40.0	18.0	41.0
43	34.39380176755522	38.0	34.0	40.0	18.0	41.0
44	34.33510754678225	38.0	34.0	40.0	18.0	41.0
45	34.28967796970538	38.0	34.0	40.0	18.0	41.0
46	34.19631785928852	38.0	34.0	40.0	18.0	41.0
47	34.10653878052384	38.0	33.0	40.0	18.0	41.0
48	34.05577317799055	38.0	33.0	40.0	18.0	41.0
49	34.023510945158634	38.0	33.0	40.0	18.0	41.0
50	33.91107756825505	38.0	33.0	40.0	18.0	41.0
51	33.26862936991655	37.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	19.0
9	46.0
10	93.0
11	86.0
12	62.0
13	57.0
14	70.0
15	149.0
16	318.0
17	713.0
18	1454.0
19	2905.0
20	5202.0
21	8711.0
22	13425.0
23	18009.0
24	24691.0
25	34836.0
26	43666.0
27	44404.0
28	41871.0
29	43107.0
30	48779.0
31	58422.0
32	73458.0
33	96867.0
34	135307.0
35	153418.0
36	166878.0
37	210247.0
38	305695.0
39	409339.0
40	152.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.70414789104726	17.037236860326896	20.99197047862578	22.266644770000056
2	38.31688423476041	21.736234058205664	23.11634182893109	16.830539878102833
3	29.25990124877925	21.720635257305567	33.40990302488034	15.609560469034836
4	24.477813408482145	24.620210383035506	33.42889958439235	17.473076624090005
5	22.556380913451367	27.578834434945023	32.8095808555865	17.055203796017107
6	22.130528500759606	32.856119852331354	31.73624950256299	13.277102144346053
7	75.9841273191633	4.737247722858215	15.787427984248815	3.4911969737296626
8	77.98128864628664	3.945724409858236	14.859788402008384	3.2131985418467437
9	72.59609865237687	6.430412616598463	16.71980383606947	4.253684894955204
10	32.28431826290106	26.882860212607024	25.98281454879053	14.850006975701394
11	25.196954166810386	26.329643333160014	31.759827888081947	16.713574611947653
12	21.08849771191846	23.90853439741523	36.939504967162726	18.063462923503586
13	21.531596323625184	24.363216277117075	37.42564185462021	16.679545544637538
14	20.372960636966482	25.82198730782715	36.33022507061933	17.474826984587047
15	19.890427432885257	25.657504902296424	37.60649527891737	16.84557238590095
16	22.15534243486471	25.28951734838918	35.897834546659205	16.657305670086906
17	22.374446384141322	24.28228784472449	34.853590066601214	18.48967570453297
18	23.136110606309433	23.607163504777713	35.33524809043392	17.92147779847894
19	21.620761746592073	26.248148607665446	34.414455506608384	17.716634139134097
20	22.323016674242982	25.61343700272387	34.51654270853872	17.54700361449443
21	22.206205851661068	25.871563694846266	34.94337326386118	16.978857189631483
22	21.175243518904153	24.408262319320325	34.7679768458195	19.64851731595603
23	17.463346678974105	33.83400507707506	31.599927308558186	17.10272093539265
24	18.688599026902526	25.77076352269317	36.27199984349718	19.26863760690713
25	19.4616405923014	27.268814702204473	34.45419898612943	18.8153457193647
26	19.46380280232716	27.009452461495933	34.861209282882456	18.665535453294464
27	16.243911705638787	39.0766951340493	28.533295717743044	16.14609744256887
28	19.130770977169636	26.19239447771559	35.35805425808653	19.318780287028233
29	19.752766727912125	25.258371227780074	34.3724468546794	20.61641518962839
30	20.038693263222815	25.35814177611139	34.83999903215361	19.763165928512187
31	20.187937236191072	26.07748845920399	33.29288627753407	20.44168802707087
32	19.08073125943071	28.40742420552939	31.949433114864316	20.562411420175582
33	19.671220521226466	25.808705160526074	32.243493678367145	22.276580639880315
34	19.59785982392403	27.01655686586627	31.546695756971708	21.83888755323799
35	20.349227807874254	26.569133834108037	31.033737168956637	22.04790118906107
36	20.70861800286956	28.10672256837603	30.122468605482645	21.062190823271763
37	21.289634725504865	27.213060572254623	30.575245681114176	20.922059021126334
38	20.645296137829565	28.33164389224575	30.159432100684853	20.863627869239835
39	21.601250375169183	27.236690438964672	29.843440549777934	21.318618636088214
40	22.23462346914243	26.73711696063285	30.576378267318145	20.45188130290658
41	20.68792256405161	26.80002697614413	30.783487099070918	21.72856336073334
42	21.3026079856594	26.62076946877074	30.98915445747319	21.08746808809667
43	20.603750816620394	26.603317344991424	31.331761784173345	21.461170054214843
44	20.407247110232042	27.736109473723232	29.91798531447543	21.9386581015693
45	20.85039720312985	26.721724084497104	29.94877106674691	22.479107645626133
46	21.217252170833127	27.46459767191758	29.23745544946426	22.080694707785035
47	20.597727517262932	27.234579710130006	30.61982839259762	21.54786438000944
48	20.840718739205037	26.768829374343937	30.537767374001074	21.852684512449954
49	21.266004858794815	26.08001103756737	30.631874991312547	22.022109112325268
50	20.158798882034453	26.495412768467975	30.584512295510276	22.761276053987295
51	20.444879860918412	25.96649501121518	29.933635596566617	23.65498953129979
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1917.0
1	2268.5
2	2620.0
3	21050.5
4	39481.0
5	28561.5
6	17642.0
7	17803.0
8	17964.0
9	18038.0
10	18112.0
11	18164.0
12	18216.0
13	18038.0
14	17860.0
15	17463.0
16	17066.0
17	16591.0
18	16116.0
19	15593.0
20	15070.0
21	14983.0
22	14896.0
23	15741.0
24	16586.0
25	17587.0
26	20427.5
27	22267.0
28	26054.0
29	29841.0
30	34265.5
31	38690.0
32	43989.0
33	49288.0
34	54834.5
35	60381.0
36	68326.5
37	76272.0
38	82601.5
39	88931.0
40	95572.5
41	102214.0
42	106155.0
43	110096.0
44	116122.5
45	122149.0
46	129019.0
47	135889.0
48	145470.0
49	155051.0
50	157509.0
51	159967.0
52	154306.5
53	148646.0
54	132921.5
55	117197.0
56	100877.5
57	84558.0
58	73316.0
59	62074.0
60	54320.0
61	46566.0
62	39966.0
63	33366.0
64	27522.5
65	21679.0
66	18511.5
67	15344.0
68	12689.0
69	10034.0
70	7997.5
71	5961.0
72	5423.5
73	4886.0
74	4244.0
75	3136.0
76	2670.0
77	2008.5
78	1347.0
79	1087.5
80	828.0
81	555.0
82	282.0
83	226.0
84	170.0
85	113.5
86	57.0
87	36.0
88	15.0
89	9.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1942457.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.70229891322388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94905677854946	27.82960525111633
2	8.085661647359697	5.77353418090452
3	3.1077141901584486	3.328576228617132
4	1.7886610596383463	2.5543724722260803
5	1.11054705043528	1.9824541375919738
6	0.8452720919726338	1.8106894134367821
7	0.6429894287974456	1.6069340549478641
8	0.5404640924230504	1.5436648463641602
9	0.47927526072874704	1.5400105758225933
>10	4.98267400868985	38.446852711119206
>50	0.4398002997239129	9.950289814776502
>100	0.025221044874841683	1.501616206610038
>500	9.398988170443842E-4	0.2581617132367131
>1k	0.001566498028407307	0.8273926312010256
>5k	0.0	0.0
>10k+	1.5664980284073071E-4	1.0458457620291453
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18700	0.962698273372332	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTCCAGGTTCGTATGCCGTCT	2379	0.12247375360175283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0296238217885904E-4	0.0	0.0	0.06718295437170553	0.0
2	1.0296238217885904E-4	0.0	0.0	0.29133206037508164	0.0
3	1.0296238217885904E-4	0.0	0.0	0.46775810223855663	0.0
4	1.0296238217885904E-4	0.0	0.0	0.6519578039565355	0.0
5	1.0296238217885904E-4	0.0	0.0	1.1314021365723925	0.0
6	1.0296238217885904E-4	0.0	0.0	1.6965111711610605	0.0
7	1.0296238217885904E-4	0.0	0.0	2.227333732484168	0.0
8	1.0296238217885904E-4	0.0	0.0	3.0243140517396268	0.0
9	1.0296238217885904E-4	0.0	0.0	3.446253893908591	0.0
10	1.5444357326828856E-4	0.0	0.0	4.199732606693481	0.0
11	1.5444357326828856E-4	0.0	0.0	4.971693067079477	0.0
12	2.0592476435771809E-4	0.0	0.0	5.621797548156793	0.0
13	2.0592476435771809E-4	0.0	0.0	5.92980951444485	0.0
14	2.0592476435771809E-4	0.0	0.0	6.089967499924065	0.0
15	2.0592476435771809E-4	0.0	0.0	6.2691735261063695	0.0
16	2.0592476435771809E-4	0.0	0.0	6.627740022044246	0.0
17	2.0592476435771809E-4	0.0	0.0	7.008597873723845	0.0
18	2.0592476435771809E-4	0.0	0.0	7.486394808224841	0.0
19	2.0592476435771809E-4	0.0	0.0	7.7395278248115655	0.0
20	2.0592476435771809E-4	0.0	0.0	7.984012001295267	0.0
21	2.574059554471476E-4	0.0	0.0	8.27966848172186	0.0
22	2.574059554471476E-4	0.0	0.0	8.592416717590146	0.0
23	2.574059554471476E-4	0.0	0.0	8.941716599131924	0.0
24	2.574059554471476E-4	0.0	0.0	9.21647171597621	0.0
25	3.0888714653657713E-4	0.0	0.0	9.468369183976789	0.0
26	3.0888714653657713E-4	0.0	0.0	9.708477459217887	0.0
27	3.0888714653657713E-4	0.0	0.0	9.95996307768975	0.0
28	3.6036833762600665E-4	0.0	0.0	10.264371360601547	0.0
29	3.6036833762600665E-4	0.0	0.0	10.581495497712433	0.0
30	3.6036833762600665E-4	0.0	0.0	10.937899783624554	0.0
31	3.6036833762600665E-4	0.0	0.0	11.27489566049596	0.0
32	3.6036833762600665E-4	0.0	0.0	11.617245581240665	0.0
33	3.6036833762600665E-4	0.0	0.0	11.955013675978412	0.0
34	3.6036833762600665E-4	0.0	0.0	12.291082891410209	0.0
35	3.6036833762600665E-4	0.0	0.0	12.637757232206427	0.0
36	3.6036833762600665E-4	0.0	0.0	12.972539417860988	0.0
37	3.6036833762600665E-4	0.0	0.0	13.31797821007106	0.0
38	3.6036833762600665E-4	0.0	0.0	13.680148389385197	0.0
39	3.6036833762600665E-4	0.0	0.0	14.044017448005285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	25	3.8918028E-5	45.000004	1
CGTTTTT	21315	0.0	43.501053	1
GTAAGCG	70	0.0	41.785713	1
CGGCGAA	110	0.0	38.863636	17
GCTATCG	35	6.2505187E-6	38.571426	1
CTAACGC	300	0.0	37.5	25
TTTACGG	265	0.0	37.35849	2
GTTTTTT	25145	0.0	37.17936	2
GCGAAAG	685	0.0	36.788322	1
TAACGCC	260	0.0	36.346157	26
TAGCCCG	25	0.0021074654	36.000004	1
CGTTGAT	75	1.8189894E-12	36.0	25
CGAAAGG	735	0.0	35.816326	2
AACGCCT	255	0.0	35.294117	27
TATTGCG	205	0.0	35.121952	1
TCTAACG	335	0.0	34.92537	24
TTGCGCG	220	0.0	34.772728	1
ACGTAGG	195	0.0	34.615383	2
CTACGCG	85	0.0	34.411762	1
TCGCACG	40	1.5611202E-5	33.75	1
>>END_MODULE