FastQCFastQC Report
Sat 18 Jun 2016
SRR3552611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623205
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.27133928643062877No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14490.23250776229330636No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC9380.15051227124300992TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8370.13430572604520183No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC7120.11424812060237001TruSeq Adapter, Index 14 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC6580.10558323505106665No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG253.8887418E-545.0000041
CGTATGG253.8887418E-545.0000042
TGTTACG253.8887418E-545.0000041
CTTAACG207.0307986E-445.024
CGTGGTA207.0307986E-445.045
AACGTAA207.0307986E-445.026
TACGGGT351.2107375E-745.04
TACGCGT207.0307986E-445.015
CCGTAAT207.0307986E-445.014
TTAACGC207.0307986E-445.025
GACTCGT207.0307986E-445.010
GTAAGCG600.044.9999961
CGTAAGG850.042.352942
ATGGGTC603.6379788E-1241.2499965
GTAGTAG1050.040.7142871
CGTTAGG451.925946E-840.02
TATGGGA3150.038.571434
TATGGGC2800.038.5714264
GGATCGT700.038.5714268
ACTCGAG356.2436866E-638.5714261