##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552611_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623205
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.756850474562945	31.0	31.0	34.0	30.0	34.0
2	31.871074526038782	33.0	31.0	34.0	30.0	34.0
3	31.908208374451423	33.0	31.0	34.0	30.0	34.0
4	35.584079075103695	37.0	35.0	37.0	33.0	37.0
5	35.48877817090685	37.0	35.0	37.0	33.0	37.0
6	35.417422838391865	37.0	35.0	37.0	33.0	37.0
7	35.7803740342263	37.0	35.0	37.0	35.0	37.0
8	35.59209569884709	37.0	35.0	37.0	35.0	37.0
9	37.40627562359095	39.0	37.0	39.0	35.0	39.0
10	36.90813616707183	39.0	37.0	39.0	33.0	39.0
11	36.783779013326274	39.0	37.0	39.0	32.0	39.0
12	36.65233751333831	39.0	35.0	39.0	32.0	39.0
13	36.48410394653445	39.0	35.0	39.0	32.0	39.0
14	37.60274227581614	40.0	36.0	41.0	33.0	41.0
15	37.77252749897706	40.0	36.0	41.0	33.0	41.0
16	37.83636845018894	40.0	36.0	41.0	33.0	41.0
17	37.782922152421754	40.0	36.0	41.0	33.0	41.0
18	37.774807647563804	39.0	36.0	41.0	33.0	41.0
19	37.67834179764283	39.0	36.0	41.0	33.0	41.0
20	37.610829502330695	39.0	36.0	41.0	33.0	41.0
21	37.44863247246091	39.0	35.0	41.0	32.0	41.0
22	37.50781364077631	39.0	35.0	41.0	33.0	41.0
23	33.53725339174108	39.0	34.0	41.0	0.0	41.0
24	35.133187313965706	39.0	34.0	41.0	18.0	41.0
25	36.14307330653637	38.0	34.0	40.0	29.0	41.0
26	36.74190675620382	38.0	35.0	40.0	31.0	41.0
27	30.70188782182428	38.0	32.0	40.0	0.0	41.0
28	33.19280333116711	38.0	31.0	40.0	16.0	41.0
29	35.10412785520013	38.0	32.0	40.0	25.0	41.0
30	36.11276706701647	38.0	35.0	40.0	30.0	41.0
31	36.50310411501833	38.0	35.0	40.0	30.0	41.0
32	35.00575091663257	38.0	35.0	40.0	26.0	41.0
33	35.671129082725585	38.0	35.0	40.0	26.0	41.0
34	36.08137290297735	38.0	35.0	40.0	29.0	41.0
35	36.26042313524442	38.0	35.0	40.0	30.0	41.0
36	36.30244462095137	38.0	35.0	40.0	30.0	41.0
37	36.32851790341862	38.0	35.0	40.0	30.0	41.0
38	36.274511597307466	38.0	35.0	40.0	30.0	41.0
39	36.23838062916697	38.0	35.0	40.0	30.0	41.0
40	36.107804013125694	38.0	35.0	40.0	30.0	41.0
41	36.00246467855681	38.0	35.0	40.0	30.0	41.0
42	35.929304161551975	38.0	35.0	40.0	30.0	41.0
43	35.91287297117321	38.0	35.0	40.0	30.0	41.0
44	35.84217713272518	38.0	35.0	40.0	30.0	41.0
45	35.771245416837154	38.0	35.0	40.0	29.0	41.0
46	35.634577707175005	38.0	34.0	40.0	29.0	41.0
47	35.54035670445519	37.0	34.0	40.0	29.0	41.0
48	35.48674031819385	37.0	34.0	40.0	29.0	41.0
49	35.47046958865863	37.0	34.0	40.0	29.0	41.0
50	35.35186014232877	37.0	34.0	40.0	29.0	41.0
51	34.61401144085814	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	12.0
9	34.0
10	36.0
11	34.0
12	46.0
13	28.0
14	34.0
15	41.0
16	116.0
17	154.0
18	275.0
19	507.0
20	857.0
21	1298.0
22	1924.0
23	2725.0
24	3513.0
25	4805.0
26	6226.0
27	7869.0
28	9631.0
29	12172.0
30	16125.0
31	20065.0
32	26124.0
33	35599.0
34	52795.0
35	61584.0
36	58361.0
37	73502.0
38	100913.0
39	125748.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60188060108632	19.502410924174228	23.994512239150843	14.90119623558861
2	30.427066535088777	25.57104002695742	26.53877937436317	17.46311406359063
3	32.44774993782143	24.13652008568609	26.683033672708017	16.73269630378447
4	28.39739732511774	26.35874230790831	26.09927712389984	19.14458324307411
5	25.329065074895098	29.767091085597837	24.982469652842966	19.9213741866641
6	25.261831981450726	34.43120642485217	24.73856917065813	15.568392423038969
7	81.48666971542269	6.26551455781003	7.8232684269221195	4.424547299845155
8	82.97029067481809	5.337890421289945	7.095899423143267	4.59591948074871
9	77.74071132291942	7.607127670670165	9.425630410539068	5.226530595871343
10	41.80245665551464	26.86258935663225	15.582352516427179	15.752601471425937
11	32.024935615086534	26.315738801838883	22.42713071942619	19.232194863648395
12	27.637133848412642	23.32651374748277	28.77367800322526	20.262674400879323
13	24.3438354955432	26.3677281151467	28.88247045514718	20.405965934162914
14	20.154202870644493	28.931571473271234	28.410234192601152	22.50399146348312
15	19.94062948788922	26.079700900987635	32.955127125103296	21.024542486019847
16	22.17921871615279	24.41764748357282	31.028634237530188	22.3744995627442
17	22.074919167850066	24.497556983657063	28.273200632215723	25.154323216277145
18	23.80934042570262	24.32827079371956	28.67371089769819	23.18867788287963
19	24.624641971742843	25.93480475926862	27.477796230774786	21.962757038213752
20	25.926139873717318	25.262955207355525	27.02513619114096	21.785768727786202
21	24.414919649232598	26.505082597219214	26.810920965011515	22.269076788536676
22	24.049871230173057	24.856347429818438	25.700852849383427	25.392928490625078
23	19.845797129355507	34.94708803684181	24.02965316388668	21.177461669916
24	22.37867154467631	25.536380484752208	29.46686884732953	22.61807912324195
25	22.302613104837093	26.55851605811892	27.66553541771969	23.473335419324297
26	21.63333092642068	27.297277781789298	27.48469604704712	23.5846952447429
27	18.69320689018862	39.0083519869064	23.33501817219053	18.963422950714452
28	21.245978450108712	27.270320360074134	28.95435691305429	22.529344276762863
29	21.81657720974639	26.58370841055511	27.09349251049013	24.506221869208368
30	22.543304370151073	26.89243507353118	27.520799736844214	23.043460819473527
31	24.376569507625902	27.03877536284208	25.343827472501022	23.240827657030994
32	22.334705273545623	30.54211695990886	25.4317600147624	21.69141775178312
33	23.44493385001725	25.874631942940123	26.32055262714516	24.359881579897465
34	23.080848196018966	26.538458452676082	27.262778700427624	23.11791465087732
35	22.313203520510907	26.04520181962597	27.320705064946527	24.3208895949166
36	24.85763111656678	27.615632095377922	26.69827745284457	20.828459335210724
37	23.730714612366718	27.386975393329642	26.803058383677925	22.079251610625718
38	23.370800940300544	27.887131842652096	26.65944592870725	22.082621288340114
39	24.59896823677602	24.91507609855505	27.21688689917443	23.2690687654945
40	24.587575516884492	24.804518577354163	28.69553357241999	21.912372333341356
41	21.51779911906997	26.229571328856476	27.837709902840956	24.414919649232598
42	23.50045330188301	25.932237385771938	27.03572660681477	23.531582705530283
43	23.219807286526905	25.123193812629875	27.92002631557834	23.73697258526488
44	22.95408412962027	25.88297590680434	27.025457112828043	24.137482850747347
45	23.157869400919438	25.391163421346107	27.48180775186335	23.9691594258711
46	23.344806283646633	25.981659325583074	27.500421209714297	23.173113181055992
47	22.889739331359664	25.97957333461702	28.395471794995224	22.73521553902809
48	23.06399980744699	25.090138878860085	28.47650452098427	23.369356792708658
49	23.771150744939465	24.79168170987075	27.965436734300912	23.47173081088887
50	22.56608980993413	24.97043508957727	28.598615222920227	23.864859877568374
51	21.874984956795917	24.77916576407442	27.096541266517438	26.24930801261222
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1669.0
1	1957.0
2	2245.0
3	3147.5
4	4050.0
5	2873.0
6	1696.0
7	1680.5
8	1665.0
9	1725.5
10	1786.0
11	1786.5
12	1787.0
13	1853.5
14	1920.0
15	1842.5
16	1765.0
17	1758.0
18	1751.0
19	1935.0
20	2119.0
21	2004.0
22	1889.0
23	2337.5
24	2786.0
25	3408.5
26	5026.5
27	6022.0
28	6606.0
29	7190.0
30	8193.5
31	9197.0
32	10517.0
33	11837.0
34	13216.5
35	14596.0
36	16465.5
37	18335.0
38	19873.0
39	21411.0
40	23437.5
41	25464.0
42	29482.0
43	33500.0
44	36525.5
45	39551.0
46	43274.5
47	46998.0
48	49749.0
49	52500.0
50	52296.5
51	52093.0
52	49111.0
53	46129.0
54	42771.0
55	39413.0
56	36037.0
57	32661.0
58	31118.5
59	29576.0
60	27853.0
61	26130.0
62	23505.0
63	20880.0
64	18406.5
65	15933.0
66	13662.5
67	11392.0
68	10241.5
69	9091.0
70	8326.5
71	7562.0
72	6394.0
73	5226.0
74	4464.5
75	3069.5
76	2436.0
77	1964.0
78	1492.0
79	1159.5
80	827.0
81	692.0
82	557.0
83	369.5
84	182.0
85	130.0
86	78.0
87	54.5
88	31.0
89	41.0
90	51.0
91	26.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	623205.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.214974593879234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.878958111505	23.800087585844018
2	10.175690551535144	6.556192251857633
3	4.071729628744039	3.935119996294037
4	2.1219300635261753	2.7343169234593714
5	1.3300778371874316	2.1424211866437473
6	0.8436634363594068	1.6307157700861885
7	0.6211219493906034	1.400659947352332
8	0.45600388080589477	1.1752122747897789
9	0.391602922177207	1.135393036994483
>10	5.612541445787189	43.18310726061368
>50	0.4558298478219986	9.12704776550741
>100	0.03686492758334541	1.9922837819067771
>500	0.002989048182433412	0.6835135030724153
>1k	9.963493941444706E-4	0.5039287155781601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1691	0.27133928643062877	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1449	0.23250776229330636	No Hit
GCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	938	0.15051227124300992	TruSeq Adapter, Index 14 (95% over 21bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	837	0.13430572604520183	No Hit
CCTGTCTCTTATACACATCTGACGCATGCTGTGTCGTATGCCGTCTTCTGC	712	0.11424812060237001	TruSeq Adapter, Index 14 (95% over 21bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	658	0.10558323505106665	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1124830513234008	0.0
2	0.0	0.0	0.0	0.5401111993645751	0.0
3	0.0	0.0	0.0	0.7317014465545045	0.0
4	0.0	0.0	0.0	0.9488049678677161	0.0
5	0.0	0.0	0.0	1.5731581100921848	0.0
6	0.0	0.0	0.0	2.0989882943814635	0.0
7	0.0	0.0	0.0	2.634767050970387	0.0
8	0.0	0.0	0.0	3.502699753692605	0.0
9	0.0	0.0	0.0	3.9020867932702723	0.0
10	0.0	0.0	0.0	4.738248248971045	0.0
11	0.0	0.0	0.0	5.7481887982285125	0.0
12	0.0	0.0	0.0	6.573278455724842	0.0
13	0.0	0.0	0.0	6.890349082565127	0.0
14	0.0	0.0	0.0	7.039096284529168	0.0
15	0.0	0.0	0.0	7.232772522685152	0.0
16	0.0	0.0	0.0	7.648526568304169	0.0
17	0.0	0.0	0.0	8.141462279667204	0.0
18	0.0	0.0	0.0	8.744153208013415	0.0
19	0.0	0.0	0.0	9.032501343859565	0.0
20	0.0	0.0	0.0	9.31154275078024	0.0
21	1.604608435426545E-4	0.0	0.0	9.644819922818334	0.0
22	1.604608435426545E-4	0.0	0.0	9.992057188244639	0.0
23	1.604608435426545E-4	0.0	0.0	10.37234938744073	0.0
24	1.604608435426545E-4	0.0	0.0	10.666634574497959	0.0
25	1.604608435426545E-4	0.0	0.0	10.912621047648848	0.0
26	4.8138253062796353E-4	0.0	0.0	11.170000240691266	0.0
27	6.41843374170618E-4	0.0	0.0	11.429625885543281	0.0
28	6.41843374170618E-4	0.0	0.0	11.702730241252878	0.0
29	6.41843374170618E-4	0.0	0.0	11.985462247575036	0.0
30	6.41843374170618E-4	0.0	0.0	12.33446458228031	0.0
31	6.41843374170618E-4	0.0	0.0	12.654102582617277	0.0
32	6.41843374170618E-4	0.0	0.0	12.973259200423616	0.0
33	6.41843374170618E-4	0.0	0.0	13.295304113413724	0.0
34	6.41843374170618E-4	0.0	0.0	13.608042297478358	0.0
35	6.41843374170618E-4	0.0	0.0	13.972609334007268	0.0
36	8.023042177132725E-4	0.0	0.0	14.302195906643881	0.0
37	9.627650612559271E-4	0.0	0.0	14.654888840750635	0.0
38	9.627650612559271E-4	0.0	0.0	14.98399403085662	0.0
39	9.627650612559271E-4	0.0	0.0	15.311815534214263	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	25	3.8887418E-5	45.000004	1
CGTATGG	25	3.8887418E-5	45.000004	2
TGTTACG	25	3.8887418E-5	45.000004	1
CTTAACG	20	7.0307986E-4	45.0	24
CGTGGTA	20	7.0307986E-4	45.0	45
AACGTAA	20	7.0307986E-4	45.0	26
TACGGGT	35	1.2107375E-7	45.0	4
TACGCGT	20	7.0307986E-4	45.0	15
CCGTAAT	20	7.0307986E-4	45.0	14
TTAACGC	20	7.0307986E-4	45.0	25
GACTCGT	20	7.0307986E-4	45.0	10
GTAAGCG	60	0.0	44.999996	1
CGTAAGG	85	0.0	42.35294	2
ATGGGTC	60	3.6379788E-12	41.249996	5
GTAGTAG	105	0.0	40.714287	1
CGTTAGG	45	1.925946E-8	40.0	2
TATGGGA	315	0.0	38.57143	4
TATGGGC	280	0.0	38.571426	4
GGATCGT	70	0.0	38.571426	8
ACTCGAG	35	6.2436866E-6	38.571426	1
>>END_MODULE