FastQCFastQC Report
Sat 18 Jun 2016
SRR3552608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793585
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC16260.2048929856285086Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16140.20338086027331664No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC15640.19708033796001687Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.16998809201282786No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT10480.1320589476867632Illumina Single End Adapter 2 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC9990.12588443581972947No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8290.10446265995451022No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG207.031964E-445.0000041
CACGGCA207.031964E-445.00000412
TGATTCG207.031964E-445.0000041
CTATTCG207.031964E-445.00000425
CGACTCG207.031964E-445.0000042
ATACCGG406.8102963E-945.0000042
ACGAACA207.031964E-445.00000435
TCGTTGC453.8380676E-1045.016
ACGATAG253.8897102E-545.01
CGGTCTA253.8897102E-545.031
ACGCATA302.1646501E-644.99999617
ACGCATT1650.040.90909217
TAACGCA501.0804797E-940.526
CGCTACA1950.040.38461728
ACGACCA4100.040.06097428
ACACGAC3550.039.92957726
ACCGCTA1700.039.70588326
CGACCAA4200.039.64285729
CGACAAT403.4564255E-739.37500420
TACGAAG403.4564255E-739.3750041