##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793585
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673522054978356	31.0	31.0	34.0	30.0	34.0
2	31.791285117536244	31.0	31.0	34.0	30.0	34.0
3	31.827752540685623	33.0	31.0	34.0	30.0	34.0
4	35.50653301158666	37.0	35.0	37.0	33.0	37.0
5	35.40452377502095	37.0	35.0	37.0	33.0	37.0
6	35.34339736764178	37.0	35.0	37.0	33.0	37.0
7	35.74705419079242	37.0	35.0	37.0	35.0	37.0
8	35.58333133816794	37.0	35.0	37.0	35.0	37.0
9	37.38235601731383	39.0	37.0	39.0	35.0	39.0
10	36.81433368826276	39.0	37.0	39.0	32.0	39.0
11	36.69308517676115	39.0	35.0	39.0	32.0	39.0
12	36.5468185512579	39.0	35.0	39.0	32.0	39.0
13	36.372562485430045	39.0	35.0	39.0	32.0	39.0
14	37.423087633964855	39.0	36.0	41.0	32.0	41.0
15	37.586382051072036	39.0	36.0	41.0	33.0	41.0
16	37.65547861917753	39.0	36.0	41.0	33.0	41.0
17	37.59933970526156	39.0	36.0	41.0	33.0	41.0
18	37.582134238928404	39.0	36.0	41.0	33.0	41.0
19	37.54580416716546	39.0	36.0	41.0	33.0	41.0
20	37.48478360856115	39.0	35.0	41.0	32.0	41.0
21	37.32959922377565	39.0	35.0	41.0	32.0	41.0
22	37.39219743316721	39.0	35.0	41.0	32.0	41.0
23	33.47174782789493	39.0	34.0	40.0	0.0	41.0
24	35.06676033443173	38.0	34.0	41.0	18.0	41.0
25	36.09714271313092	38.0	34.0	40.0	28.0	41.0
26	36.68237554893301	38.0	35.0	40.0	30.0	41.0
27	30.652188486425526	38.0	31.0	40.0	0.0	41.0
28	33.16074648588368	38.0	31.0	40.0	16.0	41.0
29	35.04966449718682	38.0	32.0	40.0	25.0	41.0
30	36.02873416206204	38.0	34.0	40.0	30.0	41.0
31	36.41718530466176	38.0	35.0	40.0	30.0	41.0
32	34.95207822728504	38.0	35.0	40.0	26.0	41.0
33	35.58668825645646	38.0	35.0	40.0	26.0	41.0
34	36.013968257968585	38.0	35.0	40.0	29.0	41.0
35	36.1822085850917	38.0	35.0	40.0	30.0	41.0
36	36.21969669285584	38.0	35.0	40.0	30.0	41.0
37	36.258236987846296	38.0	35.0	40.0	30.0	41.0
38	36.21583195246886	38.0	35.0	40.0	30.0	41.0
39	36.216096574406016	38.0	35.0	40.0	30.0	41.0
40	36.147051670583494	38.0	35.0	40.0	30.0	41.0
41	36.05516359306186	38.0	35.0	40.0	30.0	41.0
42	35.98026046359243	38.0	35.0	40.0	30.0	41.0
43	35.9375416622038	38.0	35.0	40.0	30.0	41.0
44	35.85840584184429	38.0	35.0	40.0	30.0	41.0
45	35.78114505692522	38.0	35.0	40.0	29.0	41.0
46	35.64059678547351	38.0	34.0	40.0	29.0	41.0
47	35.54576762413604	38.0	34.0	40.0	29.0	41.0
48	35.531568767050786	37.0	34.0	40.0	29.0	41.0
49	35.50721472810096	37.0	34.0	40.0	29.0	41.0
50	35.39328742352741	37.0	34.0	40.0	28.0	41.0
51	34.67275087104721	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	40.0
10	52.0
11	64.0
12	41.0
13	42.0
14	47.0
15	63.0
16	107.0
17	222.0
18	373.0
19	632.0
20	1075.0
21	1635.0
22	2374.0
23	3372.0
24	4659.0
25	6097.0
26	7985.0
27	10129.0
28	12724.0
29	16204.0
30	21221.0
31	26907.0
32	34286.0
33	46608.0
34	68855.0
35	77227.0
36	73576.0
37	93466.0
38	128930.0
39	154512.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.459471890219696	19.137962537094324	23.79707277733324	14.605492795352735
2	30.483691097992022	24.64739126873618	25.9013212195291	18.967596413742697
3	32.100909165369806	25.355443966304808	26.056314068436276	16.48733279988911
4	28.711984223492127	26.48122129324521	25.60985905731585	19.196935425946812
5	25.464821033663693	29.80487282395711	24.750845845120562	19.97946029725864
6	24.733078372197056	34.57323412110864	24.69615731144112	15.997530195253187
7	81.37086764492777	6.146663558408992	7.78895770459371	4.693511092069532
8	82.69536344562964	5.0349994014503805	7.12563871544951	5.143998437470467
9	77.22058758671095	7.488296779803045	9.716917532463441	5.574198101022574
10	41.99814764643989	25.691513826496216	16.691973764625086	15.618364762438805
11	31.544069003320374	25.700460568181104	23.319619196431386	19.43585123206714
12	27.24862491100512	23.2854703654933	29.092031729430367	20.37387299407121
13	24.923732177397508	24.92587435498403	30.661743858565877	19.48864960905259
14	20.948606639490414	28.36419539179798	29.924204716571	20.762993252140603
15	20.82662852750493	25.660893288053582	32.84827712217343	20.664201062268063
16	23.71781220663193	24.89311163895487	30.196009249166757	21.193066905246443
17	23.26278848516542	24.433551541422784	28.212100783154924	24.091559190256874
18	24.465054152989282	23.9739914438907	29.493627021680098	22.06732738143992
19	24.446404606941915	26.31690367131435	27.255933516888547	21.98075820485518
20	25.575080174146436	26.066394904137553	26.63734823616878	21.721176685547235
21	24.326442662096685	27.2783633763239	27.84503235318208	20.550161608397335
22	23.304875974218263	25.240648449756485	27.126772809465905	24.327702766559348
23	20.406005657869038	34.42277764826704	24.480175406541203	20.69104128732272
24	22.931255001039585	24.583629982925583	29.153650837654443	23.331464178380386
25	22.62895594044746	25.845750612725794	27.306589716287483	24.21870373053926
26	21.992981218142983	26.959304926378397	27.504678137817624	23.54303571766099
27	18.48170013294102	39.09789121518174	22.597957370666027	19.82245128121121
28	20.67869226358865	26.61050801111412	28.921035553847414	23.78976417144981
29	22.35752943919051	26.006539942161204	28.269057504867153	23.366873113781132
30	23.347215484163637	26.044217065594736	27.985407990322397	22.623159459919226
31	24.241511621313407	26.410403422443718	26.922257855176195	22.425827101066677
32	24.211269114209568	29.46477062948518	25.771656470321393	20.55230378598386
33	23.492001486923268	26.62399112886458	26.041318825330617	23.84268855888153
34	22.777018214810006	25.856965542443465	29.401891416798453	21.96412482594807
35	22.943604024773652	26.46357983076797	28.39342981533169	22.199386329126686
36	23.704581109774	28.565308064038508	26.640246476432893	21.089864349754595
37	23.455962499291193	27.118330109566084	28.236294788837995	21.18941260230473
38	22.577291657478405	28.28833710314585	26.908900747872	22.225470491503746
39	22.47522319600295	26.161406780622116	27.390512673500634	23.972857349874303
40	24.803896242998544	24.919951864009526	27.77522256595072	22.50092932704121
41	21.92077723243257	25.35645204987493	27.120598297598868	25.602172420093627
42	23.249809409200022	25.31045823698785	26.989295412589705	24.450436941222428
43	23.07654504558428	24.221727981249646	27.91698431799996	24.784742655166113
44	22.542134742970195	25.17247679832658	28.213360887617583	24.072027571085645
45	23.110189834737298	24.614628552707018	27.618843602134618	24.656338010421063
46	23.52552026563002	25.844238487370603	27.585324823427865	23.044916423571514
47	21.78859227429954	26.346642136633125	28.710976139922	23.153789449145336
48	22.634374389636903	24.478537270739746	28.364321402244247	24.52276693737911
49	22.8100329517317	24.342698009665	29.11269744261799	23.734571595985308
50	21.249393574727346	24.76445497331729	29.487578520259326	24.49857293169604
51	21.08973833930833	23.980669997542797	27.757707113919743	27.17188454922913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1829.0
1	2075.0
2	2321.0
3	3032.0
4	3743.0
5	2731.5
6	1720.0
7	1715.5
8	1711.0
9	1716.0
10	1721.0
11	1812.0
12	1903.0
13	1961.5
14	2020.0
15	2104.5
16	2189.0
17	2023.5
18	1858.0
19	2027.0
20	2196.0
21	2324.0
22	2452.0
23	2791.0
24	3130.0
25	3837.0
26	5375.5
27	6207.0
28	6659.0
29	7111.0
30	9512.0
31	11913.0
32	12646.5
33	13380.0
34	15811.0
35	18242.0
36	19578.0
37	20914.0
38	23972.0
39	27030.0
40	30818.0
41	34606.0
42	41225.5
43	47845.0
44	55951.0
45	64057.0
46	67189.5
47	70322.0
48	71887.5
49	73453.0
50	68435.0
51	63417.0
52	59025.5
53	54634.0
54	50890.5
55	47147.0
56	43757.0
57	40367.0
58	38553.0
59	36739.0
60	34430.0
61	32121.0
62	29425.5
63	26730.0
64	23301.5
65	19873.0
66	16919.0
67	13965.0
68	11560.0
69	9155.0
70	8094.0
71	7033.0
72	6246.5
73	5460.0
74	4517.0
75	2757.5
76	1941.0
77	1783.0
78	1625.0
79	1174.0
80	723.0
81	553.5
82	384.0
83	275.5
84	167.0
85	124.5
86	82.0
87	54.5
88	27.0
89	14.0
90	1.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	793585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.832374878316784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.43161621658535	23.375027353270305
2	10.715503140967408	6.821998259861109
3	4.255870333685044	4.0642337968610835
4	2.270971792309804	2.8916170172355455
5	1.3101448505162296	2.085251101326446
6	0.9134913928604916	1.7447160279390552
7	0.6130832065305348	1.3661126123316345
8	0.4666345023869726	1.1883267528911134
9	0.41566151898152925	1.1908343965239563
>10	4.86150162346961	37.527199392993715
>50	0.6979949346905753	14.13005170726824
>100	0.04390158546441337	2.287655492805496
>500	0.0016110673564922336	0.40373407761276164
>1k	0.002013834195615292	0.9232420110795481
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1626	0.2048929856285086	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1614	0.20338086027331664	No Hit
CCTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGC	1564	0.19708033796001687	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1349	0.16998809201282786	No Hit
CTGTCTCTTATACACATCTGACGCGATTATGCTCGTATGCCGTCTTCTGCT	1048	0.1320589476867632	Illumina Single End Adapter 2 (95% over 22bp)
ATTCCTCCAGATCTCTACGCATTTCACCGCTACACCTGGAATTCTACCCCC	999	0.12588443581972947	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	829	0.10446265995451022	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7803133879798633E-4	0.0	0.0	0.1576390682787603	0.0
2	3.7803133879798633E-4	0.0	0.0	0.7849190697908857	0.0
3	3.7803133879798633E-4	0.0	0.0	1.0634021560387357	0.0
4	3.7803133879798633E-4	0.0	0.0	1.3716237076053606	0.0
5	3.7803133879798633E-4	0.0	0.0	2.2583592179791703	0.0
6	3.7803133879798633E-4	0.0	0.0	2.9742245632162905	0.0
7	3.7803133879798633E-4	0.0	0.0	3.6797570518595992	0.0
8	3.7803133879798633E-4	0.0	0.0	4.842707460448471	0.0
9	3.7803133879798633E-4	0.0	0.0	5.334022190439588	0.0
10	3.7803133879798633E-4	0.0	0.0	6.368693964729676	0.0
11	3.7803133879798633E-4	0.0	0.0	7.632200709438813	0.0
12	5.040417850639818E-4	0.0	0.0	8.69018441628811	0.0
13	5.040417850639818E-4	0.0	0.0	9.085857217563335	0.0
14	5.040417850639818E-4	0.0	0.0	9.265926145277444	0.0
15	5.040417850639818E-4	0.0	0.0	9.52815388395698	0.0
16	5.040417850639818E-4	0.0	0.0	10.095704933939023	0.0
17	5.040417850639818E-4	0.0	0.0	10.698034867090481	0.0
18	5.040417850639818E-4	0.0	0.0	11.480559738402313	0.0
19	5.040417850639818E-4	0.0	0.0	11.888959594750405	0.0
20	5.040417850639818E-4	0.0	0.0	12.247081283038364	0.0
21	5.040417850639818E-4	0.0	0.0	12.669720319814513	0.0
22	5.040417850639818E-4	0.0	0.0	13.099037910242759	0.0
23	5.040417850639818E-4	0.0	0.0	13.537806284140956	0.0
24	5.040417850639818E-4	0.0	0.0	13.898826212693033	0.0
25	5.040417850639818E-4	0.0	0.0	14.221160934241448	0.0
26	5.040417850639818E-4	0.0	0.0	14.519931702338123	0.0
27	5.040417850639818E-4	0.0	0.0	14.84856694619984	0.0
28	5.040417850639818E-4	0.0	0.0	15.21424926126376	0.0
29	5.040417850639818E-4	0.0	0.0	15.597573038804917	0.0
30	5.040417850639818E-4	0.0	0.0	16.013533521928967	0.0
31	5.040417850639818E-4	0.0	0.0	16.414120730608566	0.0
32	5.040417850639818E-4	0.0	0.0	16.788371756018574	0.0
33	6.300522313299773E-4	0.0	0.0	17.193117309424952	0.0
34	7.560626775959727E-4	0.0	0.0	17.561067812521657	0.0
35	7.560626775959727E-4	0.0	0.0	17.966191397266833	0.0
36	7.560626775959727E-4	0.0	0.0	18.351909373287047	0.0
37	0.0010080835701279636	0.0	0.0	18.733973046365545	0.0
38	0.0010080835701279636	0.0	0.0	19.144641090746422	0.0
39	0.0010080835701279636	0.0	0.0	19.617180264243906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.031964E-4	45.000004	1
CACGGCA	20	7.031964E-4	45.000004	12
TGATTCG	20	7.031964E-4	45.000004	1
CTATTCG	20	7.031964E-4	45.000004	25
CGACTCG	20	7.031964E-4	45.000004	2
ATACCGG	40	6.8102963E-9	45.000004	2
ACGAACA	20	7.031964E-4	45.000004	35
TCGTTGC	45	3.8380676E-10	45.0	16
ACGATAG	25	3.8897102E-5	45.0	1
CGGTCTA	25	3.8897102E-5	45.0	31
ACGCATA	30	2.1646501E-6	44.999996	17
ACGCATT	165	0.0	40.909092	17
TAACGCA	50	1.0804797E-9	40.5	26
CGCTACA	195	0.0	40.384617	28
ACGACCA	410	0.0	40.060974	28
ACACGAC	355	0.0	39.929577	26
ACCGCTA	170	0.0	39.705883	26
CGACCAA	420	0.0	39.642857	29
CGACAAT	40	3.4564255E-7	39.375004	20
TACGAAG	40	3.4564255E-7	39.375004	1
>>END_MODULE