FastQCFastQC Report
Sat 18 Jun 2016
SRR3552607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891922
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65270.3449930811101092No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC28350.14984761528223678Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC24930.13177076010533204RNA PCR Primer, Index 16 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC22920.12114664346627399RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT22390.1183452594768706RNA PCR Primer, Index 16 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60850.041.4872631
AATTGCG1150.041.0869561
TATCGCG900.040.01
TAACGCA3500.038.57142626
TACTACG659.094947E-1238.076921
CGTAGTG301.14020084E-437.49999628
GTTTACG552.752131E-936.8181841
TAGGGCG7900.036.7405055
CTAACGC3750.036.60000225
GTTAGCG800.036.56251
CGTAAGG2900.036.465522
AGGGCGA17300.036.1560676
CGAACGC250.00210745236.025
TATAGCG1250.036.01
TCGCGCG504.8818038E-836.01
AATCGGC250.00210745236.039
ATGACGC3450.035.86956427
CGCTACA1450.035.6896628
CGCTAGG4100.035.670732
GCGATCG702.1827873E-1135.3571439
Produced by FastQC (version 0.11.5)