##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1891922
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649456478649753	31.0	31.0	34.0	30.0	34.0
2	31.781294894821244	31.0	31.0	34.0	30.0	34.0
3	31.79779187514073	33.0	31.0	34.0	30.0	34.0
4	35.49991701560635	37.0	35.0	37.0	33.0	37.0
5	35.40064600971922	37.0	35.0	37.0	33.0	37.0
6	35.33828931636717	37.0	35.0	37.0	33.0	37.0
7	35.76648244483652	37.0	35.0	37.0	35.0	37.0
8	35.63148057900907	37.0	35.0	37.0	35.0	37.0
9	37.433231919709165	39.0	37.0	39.0	35.0	39.0
10	36.76461767451301	39.0	37.0	39.0	32.0	39.0
11	36.71633291435905	39.0	37.0	39.0	32.0	39.0
12	36.70630660249207	39.0	35.0	39.0	32.0	39.0
13	36.628112046902565	39.0	35.0	39.0	32.0	39.0
14	37.75968829581769	40.0	37.0	41.0	33.0	41.0
15	37.87815248197336	40.0	37.0	41.0	33.0	41.0
16	37.91971127773767	40.0	37.0	41.0	33.0	41.0
17	37.87835280735675	40.0	37.0	41.0	33.0	41.0
18	37.84988334614218	39.0	37.0	41.0	33.0	41.0
19	37.7741148948001	39.0	37.0	41.0	33.0	41.0
20	37.68639880502473	39.0	36.0	41.0	33.0	41.0
21	37.54719962028033	39.0	36.0	41.0	32.0	41.0
22	37.63117559814834	39.0	36.0	41.0	33.0	41.0
23	33.57259125904768	39.0	35.0	41.0	0.0	41.0
24	35.27762931029926	39.0	35.0	41.0	18.0	41.0
25	36.32466190466626	39.0	34.0	41.0	29.0	41.0
26	36.921735674092275	39.0	35.0	40.0	31.0	41.0
27	30.6941311533985	38.0	31.0	40.0	0.0	41.0
28	33.320046492402966	38.0	31.0	40.0	16.0	41.0
29	35.24323254341353	38.0	32.0	40.0	25.0	41.0
30	36.224759794536986	38.0	35.0	40.0	30.0	41.0
31	36.58677049053819	38.0	35.0	40.0	30.0	41.0
32	35.03959941266078	38.0	35.0	40.0	25.0	41.0
33	35.690847719937715	39.0	35.0	40.0	25.0	41.0
34	36.06286147103316	39.0	35.0	40.0	29.0	41.0
35	36.27290818543259	39.0	35.0	40.0	30.0	41.0
36	36.30828279389954	39.0	35.0	40.0	30.0	41.0
37	36.353363405045236	39.0	35.0	40.0	30.0	41.0
38	36.33819258933508	39.0	35.0	40.0	30.0	41.0
39	36.30169954152444	39.0	35.0	40.0	30.0	41.0
40	36.24629768034834	39.0	35.0	40.0	30.0	41.0
41	36.17901953674623	39.0	35.0	40.0	30.0	41.0
42	36.15407136235003	39.0	35.0	40.0	30.0	41.0
43	36.12308329836008	39.0	35.0	40.0	30.0	41.0
44	36.069828988721525	39.0	35.0	40.0	30.0	41.0
45	36.0021745082514	38.0	35.0	40.0	29.0	41.0
46	35.897624214951776	38.0	35.0	40.0	29.0	41.0
47	35.81837359045458	38.0	35.0	40.0	29.0	41.0
48	35.79050087688604	38.0	35.0	40.0	29.0	41.0
49	35.770426053505375	38.0	35.0	40.0	29.0	41.0
50	35.68068821019048	38.0	35.0	40.0	28.0	41.0
51	34.943075877335325	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	12.0
9	58.0
10	109.0
11	91.0
12	53.0
13	74.0
14	74.0
15	103.0
16	169.0
17	395.0
18	706.0
19	1431.0
20	2482.0
21	4066.0
22	6255.0
23	8571.0
24	11828.0
25	16338.0
26	20946.0
27	24742.0
28	29078.0
29	35801.0
30	45943.0
31	59608.0
32	76560.0
33	103149.0
34	145060.0
35	163272.0
36	178064.0
37	227309.0
38	323097.0
39	406294.0
40	181.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34508610820108	19.16907779496195	22.97388581558859	15.511950281248382
2	32.20671888164522	24.44799521333332	25.228841358153243	18.11644454686821
3	30.979342700174744	24.151365648266683	27.57381118249061	17.295480469067964
4	26.84835844183851	26.18881750938992	28.281345636870864	18.681478411900702
5	24.00764936398012	30.00662818023153	27.192664391026693	18.793058064761656
6	23.09513817165824	36.389343746729516	26.32222681484755	14.1932912667647
7	81.00904794172276	5.49932819640556	9.44420541650237	4.0474184453693125
8	82.81937627449757	4.496009877785659	8.38491227439609	4.299701573320676
9	77.29520561629919	6.590335119523955	10.56592185090083	5.548537413276023
10	36.96711598046854	24.042640235696823	21.6673308941912	17.32291288964344
11	28.778512010537433	27.072522017292467	24.44677951839452	19.702186453775578
12	23.70023711336937	23.885392738178425	31.471012018465878	20.94335812998633
13	23.490820446086044	24.45861932997238	32.61260242229859	19.437957801642984
14	21.274291434847736	27.352396134724373	30.72483960755253	20.64847282287536
15	20.21790538933423	26.768281144782925	33.26141352550475	19.75239994037809
16	21.910998444967607	25.199294685510292	31.496488755878943	21.39321811364316
17	22.294048063292248	25.391903048857195	29.717609922607803	22.596438965242754
18	23.129336198849636	24.83802186348063	31.06042426696238	20.972217670707355
19	22.7446480351727	27.361381706011134	28.874129060288954	21.01984119852721
20	23.369779515223144	26.01132604832546	29.745835187708586	20.873059248742813
21	22.722818382576026	27.301019809484746	29.6971016775533	20.279060130385925
22	21.27518999197641	25.814330611938548	28.82227702833415	24.088202367750892
23	18.47475741600341	34.80624465490649	25.991927785606382	20.727070143483715
24	20.593502269121032	26.142832526922355	30.654487870007323	22.609177333949283
25	20.966139196013366	27.760605352651957	28.701976085694863	22.57127936563981
26	20.39211975969411	27.852311036078653	29.706774380761996	22.048794823465236
27	17.92161621885046	39.97559095988101	23.840253456537848	18.26253936473068
28	20.408822351027155	27.27792160564759	30.27503248019739	22.03822356312787
29	21.38972959773183	26.221799841642518	28.82090276449029	23.567567796135357
30	22.047209134414633	26.606012298604277	28.785224760851662	22.561553806129428
31	21.87051051787547	27.268724609154077	27.774770841504036	23.085994031466413
32	20.7271229997854	30.427311485357215	26.734505968005024	22.111059546852356
33	22.0252209129129	26.075493598573303	26.827955909387384	25.071329579126413
34	19.922702944413142	27.224113890530372	28.456881414772912	24.396301750283573
35	21.632762872887994	26.366943246074626	26.773196780839804	25.227097100197575
36	21.302252418440084	27.953530853808985	26.458701785803008	24.285514941947923
37	21.10425271232112	28.043492279280013	27.563979910376858	23.288275098022012
38	20.569188370345078	26.719970485041138	27.351444721294005	25.35939642331978
39	21.005728565976824	25.86264127168033	26.91934445500396	26.212285707338882
40	20.998804390455845	26.128402756561847	28.08477305089745	24.788019802084865
41	19.528236364924133	26.80586197528228	27.45810873809808	26.207792921695507
42	20.869835014339916	26.535766273662443	27.945602408555953	24.648796303441685
43	20.91867423709857	25.45797342596576	29.060500380036807	24.562851956898857
44	21.098861369549063	25.90038067108475	28.136572226550566	24.864185732815624
45	21.2056839552582	25.674789975485247	27.658064127379454	25.461461941877094
46	21.345277448013185	27.054709443624	27.057933678026895	24.54207943033592
47	20.586419524694993	26.48724418871391	28.89252305327598	24.033813233315115
48	20.681825149239767	25.738111824906102	28.342711803129305	25.237351222724826
49	21.2414676715002	25.005999190241457	28.776397758469958	24.976135379788385
50	20.32435798093156	24.54081087909544	29.25458871983094	25.880242420142057
51	20.04733810379075	24.47236196841096	27.864097991354825	27.616201936443467
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1565.0
1	2022.5
2	2480.0
3	8271.0
4	14062.0
5	9776.0
6	5490.0
7	5437.0
8	5384.0
9	5486.5
10	5589.0
11	5741.5
12	5894.0
13	5913.5
14	5933.0
15	5700.0
16	5467.0
17	5550.5
18	5634.0
19	5737.5
20	5841.0
21	6301.0
22	6761.0
23	7780.5
24	8800.0
25	10216.5
26	13622.0
27	15611.0
28	19434.5
29	23258.0
30	27770.0
31	32282.0
32	36794.5
33	41307.0
34	46338.5
35	51370.0
36	57611.0
37	63852.0
38	68695.0
39	73538.0
40	82208.5
41	90879.0
42	97958.0
43	105037.0
44	113522.5
45	122008.0
46	132731.5
47	143455.0
48	159322.5
49	175190.0
50	181818.5
51	188447.0
52	183043.5
53	177640.0
54	156715.0
55	135790.0
56	117356.5
57	98923.0
58	86702.0
59	74481.0
60	66613.5
61	58746.0
62	51884.5
63	45023.0
64	37472.5
65	29922.0
66	24359.5
67	18797.0
68	15925.0
69	13053.0
70	10817.0
71	8581.0
72	7267.0
73	5953.0
74	4921.5
75	2973.5
76	2057.0
77	1624.0
78	1191.0
79	896.0
80	601.0
81	384.0
82	167.0
83	184.0
84	201.0
85	137.5
86	74.0
87	52.5
88	31.0
89	21.5
90	12.0
91	10.0
92	8.0
93	10.5
94	13.0
95	7.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1891922.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.27682394532739
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.14224662458035	25.00495311782532
2	9.799875772180615	6.522174815138674
3	3.5455121446499107	3.539501503006057
4	1.8454094279464837	2.456374585632899
5	1.1361871894966669	1.8904350536908456
6	0.7905596578360929	1.5784388731253958
7	0.6811605382645785	1.5866801517234372
8	0.5283802744643101	1.4066253895626093
9	0.4711247218053256	1.410978098142645
>10	5.600064065692472	41.7450848140146
>50	0.41944226597391415	8.753039473157747
>100	0.035511387580925896	2.033340337928103
>500	0.0022629647641996056	0.5069715838628188
>1k	0.002088890551568867	1.1873167909547757
>5k	1.740742126307389E-4	0.3780854122340743
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6527	0.3449930811101092	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2835	0.14984761528223678	Illumina PCR Primer Index 7 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGC	2493	0.13177076010533204	RNA PCR Primer, Index 16 (96% over 25bp)
GAATCTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTC	2292	0.12114664346627399	RNA PCR Primer, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTCCACCTCGTATGCCGTCTTCTGCT	2239	0.1183452594768706	RNA PCR Primer, Index 16 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5856890506056804E-4	0.0	0.0	0.1449319792253592	0.0
2	1.5856890506056804E-4	0.0	0.0	0.6125516802489743	0.0
3	1.5856890506056804E-4	0.0	0.0	0.8950157564635328	0.0
4	1.5856890506056804E-4	0.0	0.0	1.191169614814987	0.0
5	1.5856890506056804E-4	0.0	0.0	2.097602332442881	0.0
6	1.5856890506056804E-4	0.0	0.0	2.948535933299576	0.0
7	1.5856890506056804E-4	0.0	0.0	3.6963997458669016	0.0
8	1.5856890506056804E-4	0.0	0.0	4.91494892495568	0.0
9	1.5856890506056804E-4	0.0	0.0	5.442719097298937	0.0
10	1.5856890506056804E-4	0.0	0.0	6.663646809963624	0.0
11	1.5856890506056804E-4	0.0	0.0	7.976015924546572	0.0
12	1.5856890506056804E-4	0.0	0.0	9.1453030304632	0.0
13	1.5856890506056804E-4	0.0	0.0	9.610808479419342	0.0
14	1.5856890506056804E-4	0.0	0.0	9.83914770270656	0.0
15	1.5856890506056804E-4	0.0	0.0	10.15686693214625	0.0
16	1.5856890506056804E-4	0.0	0.0	10.762018730159065	0.0
17	1.5856890506056804E-4	0.0	0.0	11.438420822845762	0.0
18	1.5856890506056804E-4	0.0	0.0	12.24474370507875	0.0
19	1.5856890506056804E-4	0.0	0.0	12.686463818275806	0.0
20	1.5856890506056804E-4	0.0	0.0	13.11058278301114	0.0
21	1.5856890506056804E-4	0.0	0.0	13.597547890452143	0.0
22	1.5856890506056804E-4	0.0	0.0	14.116491060413695	0.0
23	1.5856890506056804E-4	0.0	0.0	14.672486497857735	0.0
24	1.5856890506056804E-4	0.0	0.0	15.096975456704875	0.0
25	1.5856890506056804E-4	0.0	0.0	15.487636382472427	0.0
26	1.5856890506056804E-4	0.0	0.0	15.863021837052479	0.0
27	1.5856890506056804E-4	0.0	0.0	16.24427434111977	0.0
28	2.1142520674742406E-4	0.0	0.0	16.68271736361224	0.0
29	2.1142520674742406E-4	0.0	0.0	17.12893026245268	0.0
30	2.1142520674742406E-4	0.0	0.0	17.628686594901904	0.0
31	2.1142520674742406E-4	0.0	0.0	18.103230471446498	0.0
32	2.1142520674742406E-4	0.0	0.0	18.57544867071687	0.0
33	2.1142520674742406E-4	0.0	0.0	19.031492841671064	0.0
34	2.1142520674742406E-4	0.0	0.0	19.49842541077275	0.0
35	2.642815084342801E-4	0.0	0.0	19.968370789070587	0.0
36	3.171378101211361E-4	0.0	0.0	20.42637064318719	0.0
37	3.171378101211361E-4	0.0	0.0	20.88653760567296	0.0
38	3.6999411180799207E-4	0.0	0.0	21.353893025188142	0.0
39	3.6999411180799207E-4	0.0	0.0	21.8209313068932	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6085	0.0	41.487263	1
AATTGCG	115	0.0	41.086956	1
TATCGCG	90	0.0	40.0	1
TAACGCA	350	0.0	38.571426	26
TACTACG	65	9.094947E-12	38.07692	1
CGTAGTG	30	1.14020084E-4	37.499996	28
GTTTACG	55	2.752131E-9	36.818184	1
TAGGGCG	790	0.0	36.740505	5
CTAACGC	375	0.0	36.600002	25
GTTAGCG	80	0.0	36.5625	1
CGTAAGG	290	0.0	36.46552	2
AGGGCGA	1730	0.0	36.156067	6
CGAACGC	25	0.002107452	36.0	25
TATAGCG	125	0.0	36.0	1
TCGCGCG	50	4.8818038E-8	36.0	1
AATCGGC	25	0.002107452	36.0	39
ATGACGC	345	0.0	35.869564	27
CGCTACA	145	0.0	35.68966	28
CGCTAGG	410	0.0	35.67073	2
GCGATCG	70	2.1827873E-11	35.357143	9
>>END_MODULE